Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,178,658	T→G	V59V (GTT→GTG)	U712_10905 →	UPF0750 membrane protein ypjC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,178,658	0	T	G	100.0%	317.0 / NA	96	V59V (GTT→GTG)	U712_10905	UPF0750 membrane protein ypjC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (51/45);  total (51/45)

CAATTTTTTCATTTGGCTTGGTTCACTTCAATATGCAAAACAATCTGGCTGAGGGCGGTTTTACAGGCATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATCTCGAACCTGGTTTTGAACATTCCGATCTTTTTTATCGGCTGGCGTTTGCTTGGCAGAACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCCTGT‑CTTTGTTCCTCAGCATTTTTCAGCGATATGAAATACATATGCCTCTTCAG  >  CP006881/2178534‑2178793                                                                                                                             |                                                                                                                                         caATTTTTTCATTTGGCTTGGTTCATTTCAATATGCAGAACAATCTGGCTGAGGGCGGTTTTACAGGTATTACACTTTTGCTTTATGGCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGAt                                                                                                                            >  7:201572/1‑139 (MQ=255)          cATTTGGCTTGGTTCATTTCAATATGCAGAACAATCTGGCTGAGGGCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTa                                                                                                                    <  3:11788/138‑1 (MQ=255)              tGGCTTGGTTCATTTCAATATGCAGAACAATCTGGCTGAGGGCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGc                                                                                                               >  3:44379/1‑139 (MQ=255)                       tCATTTCAATATGCAGAACAATCTGGCTGAGGGCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTg                                                                                                      <  3:120746/139‑1 (MQ=255)                        catttCAATATGCAGAACAATCTGGCTGAGGGCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTg                                                                                                      <  6:336550/135‑1 (MQ=255)                             cAATATGCAGAACAATCTGGCTGAGGGCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCgg                                                                                                 <  7:344537/138‑1 (MQ=255)                                  tGCAGAACAATCTGGCTGAGGGCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAgga                                                                                            <  1:432214/138‑3 (MQ=255)                                  tGCAGAACAATCTGGCTGAGGGCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAgga                                                                                            <  7:411819/138‑3 (MQ=255)                                     agaaCAATCTGGCTGAGGGCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAAt                                                                                        >  7:186711/3‑139 (MQ=255)                                       aaCAATCTGGCTGAGGGCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAAtgt                                                                                      >  3:9181/1‑139 (MQ=255)                                       aaCAATCTGGCTGAGGGCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAAtgt                                                                                      >  1:302841/1‑139 (MQ=255)                                       aaCAATCTGGCTGAGGGCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAAtgt                                                                                      <  2:204121/139‑1 (MQ=255)                                       aaCAATCTGGCTGAGGGCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGGTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAAtgt                                                                                      <  7:5964/139‑1 (MQ=255)                                       aaCAATCTGGCTGAGGGCGGATTTACAGGTATTACACTTTTGCTTTATGCGCTTTTCCACATCAGCCCATCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTTCTCGGCAGGACAAtgt                                                                                      <  5:366613/139‑1 (MQ=255)                                        aCAATCTGGCTGAGGGCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAAtgtt                                                                                     <  1:71702/139‑1 (MQ=255)                                            tCTGGCTGAGGGCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCgg                                                                                                 <  7:204583/123‑1 (MQ=255)                                            tCTGGCTGAGGGCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCgg                                                                                                 >  8:204583/1‑123 (MQ=255)                                            tCTGGCTGAGGGCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAAtgtt                                                                                     <  7:27095/135‑1 (MQ=255)                                            tCTGGCTGAGGGCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAAtgtt                                                                                     >  8:27095/1‑135 (MQ=255)                                              tGGCTGAGGGCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTta                                                                               <  4:9181/139‑1 (MQ=255)                                                gCTGAGGGCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCCCATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTta                                                                               <  2:119282/137‑1 (MQ=255)                                                gCTGAGGGCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTta                                                                               <  4:44379/137‑1 (MQ=255)                                                    aGGGCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTg                                                                                                      <  1:56801/110‑1 (MQ=255)                                                    aGGGCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTg                                                                                                      >  2:56801/1‑110 (MQ=255)                                                    aGGGCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCt                                                                         >  3:199588/1‑139 (MQ=255)                                                      ggCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCt                                                                                                              <  3:175481/100‑1 (MQ=255)                                                      ggCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCt                                                                                                              >  4:175481/1‑100 (MQ=255)                                                      ggCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTg                                                                       >  1:52024/1‑139 (MQ=255)                                                       gCGGTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGt                                                                      <  2:106471/139‑1 (MQ=255)                                                          gTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTTGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCgg                                                                   <  6:305018/139‑1 (MQ=255)                                                          gTTTTACAGGTATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCgg                                                                   <  3:54902/139‑1 (MQ=255)                                                             ttACAGGTATTACTCTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCa                                                                 <  2:124905/138‑1 (MQ=255)                                                                    taTTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATAACCTTGTCGGCACTGTAGcc                                                         >  1:157441/2‑139 (MQ=255)                                                                     aTTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGCCAATGTTTGTTTATACACTTGTCGGCACTGTTGcgt                                                        >  5:37690/1‑137 (MQ=255)                                                                     aTTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAAtgt                                                                                      >  1:318612/1‑109 (MQ=255)                                                                     aTTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAAtgt                                                                                      <  2:318612/109‑1 (MQ=255)                                                                     aTTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGcct                                                        >  4:184457/1‑138 (MQ=255)                                                                     aTTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGcct                                                        >  7:42640/1‑138 (MQ=255)                                                                     aTTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTAGGCAGGACAATGTTTGTTTTTACCCTTGTCGGCACTGTAGcct                                                        >  6:367357/1‑138 (MQ=255)                                                                      ttACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTATATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGcctt                                                       <  6:282040/139‑3 (MQ=255)                                                                           cTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGGTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑Ctt                                                 <  4:126504/139‑1 (MQ=255)                                                                              ttGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCt                                                                                                    >  8:415655/1‑86 (MQ=255)                                                                              ttGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCt                                                                                                    <  7:415655/86‑1 (MQ=255)                                                                              ttGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAAtgtt                                                                                     >  5:431668/1‑101 (MQ=255)                                                                              ttGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAAtgtt                                                                                     <  6:431668/101‑1 (MQ=255)                                                                              ttGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAAtgtct                                                                                    >  8:404323/1‑100 (MQ=255)                                                                              ttGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAAtgtct                                                                                    <  7:404323/102‑3 (MQ=255)                                                                              ttGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATCCCCTTGTCGGCCCTGTAGCCTTGT‑CTTTGt                                              >  6:301424/1‑139 (MQ=255)                                                                              ttGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCgg                                                                   >  4:111391/1‑119 (MQ=255)                                                                              ttGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCgg                                                                   <  3:111391/119‑1 (MQ=255)                                                                              ttGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑GTTTGt                                              >  2:191260/1‑139 (MQ=255)                                                                              ttGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGt                                              >  5:21917/1‑139 (MQ=255)                                                                              ttGCTTTATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGt                                              >  4:218492/1‑139 (MQ=255)                                                                                    tATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCt                                          <  4:446816/137‑1 (MQ=255)                                                                                    tATGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCt                                          >  7:46255/1‑137 (MQ=255)                                                                                      tGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCATGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAg                                       <  8:42640/138‑1 (MQ=255)                                                                                      tGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAg                                       >  5:220027/1‑138 (MQ=255)                                                                                      tGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAg                                       >  5:134489/1‑138 (MQ=255)                                                                                      tGCCCTTTTCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAg                                       <  8:186711/138‑1 (MQ=255)                                                                                           ttttCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCAtctt                                 >  3:165710/1‑139 (MQ=255)                                                                                           ttttCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCAtctt                                 >  3:263733/1‑139 (MQ=255)                                                                                           ttttCCACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCAtctt                                 >  3:197525/1‑139 (MQ=255)                                                                                               ccACATCAGCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTTTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCATCTTTCa                              <  7:442495/138‑1 (MQ=255)                                                                                                       gCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCATCTTTCAGCg                           >  4:394265/1‑133 (MQ=255)                                                                                                       gCCCCTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCATCTTTCAGCg                           <  3:394265/133‑1 (MQ=255)                                                                                                        ccccTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCATCTTTCAGCGATATGaa                    <  7:162323/139‑1 (MQ=255)                                                                                                        ccccTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCATCTTTCAGCGATATGa                     >  1:270824/1‑138 (MQ=255)                                                                                                         cccTCTATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCATCTTTCAGCGATATGaaa                   <  3:184457/139‑1 (MQ=255)                                                                                                             ctATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTACTCAGCATCTTTAATCGATATTAAAtcc                >  8:162323/1‑136 (MQ=255)                                                                                                             ctATATCGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTCGTGCTTTTTTCCTCAGCATCTTTAAGCGATATTAAAtc                 >  2:74057/1‑137 (MQ=255)                                                                                                                tatcGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGCCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCATCTTTCAGCGATATGAAATCCa               <  7:246650/134‑1 (MQ=255)                                                                                                                  tcGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCATCTTTCAGCGATATGAAATCCATATg           <  4:199588/138‑1 (MQ=255)                                                                                                                  tcGAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCATCTTTCAGCGAGATGAAATCCAGATGc          >  3:422461/1‑139 (MQ=255)                                                                                                                    gAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCATCTTTCAGCg                           <  8:418388/120‑1 (MQ=255)                                                                                                                    gAACCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCATCTTTCAGCg                           >  7:418388/1‑120 (MQ=255)                                                                                                                     aaCCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGTCACTGTAGCCTTGT‑CTTTGTTCCGCAGCATCTTTCAGCGATATGAAATCCATATGcccc       >  7:428715/1‑137 (MQ=255)                                                                                                                     aaCCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGTGCTTTGTGCCTCGGCATCTTACACCGATATTAAATCAATATGccc        >  2:60543/1‑138 (MQ=255)                                                                                                                     aaCCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCATCTTTCAGCGATATGaa                    <  7:335352/126‑1 (MQ=255)                                                                                                                     aaCCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCATCTTTCAGCGATATGaa                    >  8:335352/1‑126 (MQ=255)                                                                                                                     aaCCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCATCTTTCAGCGATATGAAAt                  >  3:341298/1‑128 (MQ=255)                                                                                                                     aaCCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCATCTTTCAGCGATATGAAAt                  <  4:341298/128‑1 (MQ=255)                                                                                                                     aaCCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCATCTTTCAGCGATATGAAATCCGTATGcccc       >  5:265426/1‑137 (MQ=255)                                                                                                                     aaCCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCATCTTTCAGCGATATGAAATCCATATGcccc       >  7:89704/1‑137 (MQ=255)                                                                                                                     aaCCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCATCTTTCAGCGATATGAAATCCATATGcccc       >  5:212977/1‑137 (MQ=255)                                                                                                                     aaCCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCATCTTTCAGCGATATGAAATCCATATGcccc       >  3:220557/1‑137 (MQ=255)                                                                                                                     aaCCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCATCTTTCAGAGATATTAAATCCATATGccc        >  7:418448/1‑137 (MQ=255)                                                                                                                     aaCCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGAATCTTTCAGCGATATGAAATCCATATGccc        >  2:356263/1‑137 (MQ=255)                                                                                                                     aaCCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTCGT‑CTTTGTTCCTCAGCATCTTTCAGCGATATGAAATCCATATGcccc       <  1:74057/139‑3 (MQ=255)                                                                                                                     aaCCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCGCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCATCTTTCAGCGATATGAAATCCATATGcccc       <  5:367357/139‑3 (MQ=255)                                                                                                                     aaCCTGGTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCGCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CGTTGTTCCGCAGCCTAGTTCAGCGATATGAAATCCATATGcccc       >  6:286272/1‑137 (MQ=255)                                                                                                                          ggTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCgg                                                                   >  1:123457/1‑75 (MQ=255)                                                                                                                          ggTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCgg                                                                   <  2:123457/75‑1 (MQ=255)                                                                                                                          ggTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCATCTTTCAGCGATATGAAATCCATATGc          >  5:213851/1‑131 (MQ=255)                                                                                                                          ggTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCATCTTTCAGCGATATGAAATCCATATGc          <  6:213851/131‑1 (MQ=255)                                                                                                                          ggTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCATCTTTCAGCGATATGAAATCCATATGCCCCTTCAg  >  4:301475/1‑139 (MQ=255)                                                                                                                          ggTGTTGAACATTCCGATTTTTTTTATTGGCTGGCGTTTGCTCGGCAGGACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCTTGT‑CTTTGTTCCTCAGCATCTTTCAGCGATATGAAATCCATATGCCCCTTCAg  <  8:46255/139‑1 (MQ=255)                                                                                                                             |                                                                                                                                         CAATTTTTTCATTTGGCTTGGTTCACTTCAATATGCAAAACAATCTGGCTGAGGGCGGTTTTACAGGCATTACACTTTTGCTTTATGCCCTTTTCCACATCAGCCCCTCTATCTCGAACCTGGTTTTGAACATTCCGATCTTTTTTATCGGCTGGCGTTTGCTTGGCAGAACAATGTTTGTTTATACCCTTGTCGGCACTGTAGCCCTGT‑CTTTGTTCCTCAGCATTTTTCAGCGATATGAAATACATATGCCTCTTCAG  >  CP006881/2178534‑2178793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑