| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,179,021 | G→A | V180V (GTG→GTA) | U712_10905 → | UPF0750 membrane protein ypjC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,179,021 | 0 | G | A | 98.3% | 173.5 / ‑4.8 | 58 | V180V (GTG→GTA) | U712_10905 | UPF0750 membrane protein ypjC |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (37/20); total (38/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
TTGATATCATCGCCCGTTTGGTCAATAAGTATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTATCTTTGCTTACTTATCTCTCCTACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGAGGCGTCACC > CP006881/2178885‑2179156 | ttGATATCATCGGACGTTTAGTGAATAAGTATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGc < 5:147347/139‑1 (MQ=255)ttGATATCATCGCACGTTTAGTGAATAAGTATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGc < 4:390694/139‑1 (MQ=255)ttGATATCATCGCACGTTTAGTGAATAAGTATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGc < 8:47679/139‑1 (MQ=255) tatCATCGCACGTTTAGTGAATAAGTATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTa > 2:336919/1‑139 (MQ=255) catcGCACGTTTAGTGAATAAGTATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTAttt > 7:124453/1‑139 (MQ=255) atcGCACGTTTAGTGAATAAGTATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTg > 5:130290/1‑139 (MQ=255) atcGCACGTTTAGTGAATAAGTATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTg > 8:389594/1‑139 (MQ=255) atcGCACGTTTAGTGAATAAGTATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTg > 6:13557/1‑139 (MQ=255) atcGCACGTTTAGTGAATAAGTATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTg > 8:287636/1‑139 (MQ=255) atcGCACGTTTAGTGAATAAGTATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTAACTCTCCTATTAAGAAGCGATGTATACGATGGTAGCTGTATTTg > 8:158176/1‑139 (MQ=255) cacGTTTAGTGAATAAGTATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGc > 6:95964/3‑139 (MQ=255) gTTTAGTGAATAAGTATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGc < 2:307273/139‑1 (MQ=255) ttagtgaaTAAGTATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGc > 7:444573/7‑137 (MQ=255) aaTAAGTATTTTGGGATTCCGATGGTCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTaa > 8:345150/1‑139 (MQ=255) aTAAGTATTTTGGTATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAAt < 7:389594/139‑1 (MQ=255) tAAGTATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAAtc > 8:435570/1‑138 (MQ=255) aGTATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGa < 6:130290/139‑1 (MQ=255) tATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGAtt < 1:336919/139‑1 (MQ=255) ttttGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTAt < 7:363604/113‑1 (MQ=255) ttttGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTAt > 8:363604/1‑113 (MQ=255) ttGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTAt > 4:346229/1‑139 (MQ=255) aTTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAgg < 7:435570/139‑1 (MQ=255) ttCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAgg > 6:48058/1‑138 (MQ=255) ccGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGaa < 5:271349/138‑1 (MQ=255) cGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAgg < 5:124409/139‑1 (MQ=255) gggCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCgg < 7:158176/138‑1 (MQ=255) cAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATAc < 8:124453/139‑1 (MQ=255) aGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACg < 7:427034/139‑1 (MQ=255) aaCGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACgct > 4:268130/1‑139 (MQ=255) aaCGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACgct > 4:15381/1‑139 (MQ=255) cGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTTTTCAGGAAGGCGGATACgctgc > 6:45162/1‑139 (MQ=255) tGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTAAGGAATGCGGATACGCTGCTa > 2:27091/1‑138 (MQ=255) gCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTAtt < 6:109424/111‑1 (MQ=255) gCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTAtt > 5:109424/1‑111 (MQ=255) gCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAACGgcg > 8:381734/1‑139 (MQ=255) tCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCt < 7:147532/137‑1 (MQ=255) cGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATctct > 5:275531/1‑139 (MQ=255) catTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGaa > 4:262794/1‑138 (MQ=255) ttATCTTTGCTTACTTATCTCTCCTACAAAGCAGCGCTGTATACCCTGGTGGCTGTATTTGTTGCTGCAAGATTAATTGATTTTATTCAGGTAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACg > 4:338585/1‑139 (MQ=255) cttacttaTATCTCATATAAAGAAGCCATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTATTCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAACACGACCTGAtt < 2:271870/138‑1 (MQ=255) cTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCtt > 4:105656/1‑139 (MQ=255) taaaGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACg > 6:102927/2‑77 (MQ=255) taaaGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACg < 5:102927/76‑1 (MQ=255) aaaGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTg > 4:358385/1‑136 (MQ=255) aagaagCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTgaag > 7:137769/1‑138 (MQ=255) aatcGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATAc > 2:399954/4‑71 (MQ=255) aagCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATAc < 1:399954/71‑1 (MQ=255) aagCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAAt > 7:283046/1‑139 (MQ=255) aagCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAAt > 1:299656/1‑139 (MQ=255) aagCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAAt > 2:261583/1‑139 (MQ=255) gCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATg > 5:416155/1‑138 (MQ=255) gCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATg > 8:195773/1‑138 (MQ=255) gCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATg > 8:47271/1‑138 (MQ=255) gCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATACTTGATGaaaag > 3:23239/1‑136 (MQ=255) aTGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGCCCTGATTCAGAAAAAGATTCTTGAAGAAATGGacc > 8:379973/1‑137 (MQ=255) aTGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATGGAAc > 5:448103/1‑139 (MQ=255) aTGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATGGAAc > 8:385273/1‑139 (MQ=255) gcTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGGGGCGTc < 8:137769/138‑1 (MQ=255) ggtagCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGa > 4:321300/1‑52 (MQ=255) ggtagCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGa < 3:321300/52‑1 (MQ=255) ggtagCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGGGGCGTcccc > 1:88757/1‑139 (MQ=255) ggtagCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGGGGCGTCAc > 8:61466/1‑138 (MQ=255) | TTGATATCATCGCCCGTTTGGTCAATAAGTATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTATCTTTGCTTACTTATCTCTCCTACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGAGGCGTCACC > CP006881/2178885‑2179156 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |