Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,179,036 T→G A185A (GCT→GCG)  U712_10905 → UPF0750 membrane protein ypjC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,179,0360TG98.3% 192.3 / NA 60A185A (GCT→GCG) U712_10905UPF0750 membrane protein ypjC
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (40/19);  total (41/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTGGTCAATAAGTATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTATCTTTGCTTACTTATCTCTCCTACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGAGGCGTCACCATTTTGAAAGGACAGGGT  >  CP006881/2178900‑2179174
                                                                                                                                        |                                                                                                                                          
gTTTAGTGAATAAGTATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGc                                                                                                                                          <  2:307273/139‑1 (MQ=255)
  ttagtgaaTAAGTATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGc                                                                                                                                          >  7:444573/7‑137 (MQ=255)
        aaTAAGTATTTTGGGATTCCGATGGTCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTaa                                                                                                                                  >  8:345150/1‑139 (MQ=255)
         aTAAGTATTTTGGTATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAAt                                                                                                                                 <  7:389594/139‑1 (MQ=255)
          tAAGTATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAAtc                                                                                                                                >  8:435570/1‑138 (MQ=255)
            aGTATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGa                                                                                                                              <  6:130290/139‑1 (MQ=255)
              tATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGAtt                                                                                                                            <  1:336919/139‑1 (MQ=255)
                  ttGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTAt                                                                                                                        >  4:346229/1‑139 (MQ=255)
                       aTTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAgg                                                                                                                   <  7:435570/139‑1 (MQ=255)
                        ttCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAgg                                                                                                                   >  6:48058/1‑138 (MQ=255)
                          ccGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGaa                                                                                                                 <  5:271349/138‑1 (MQ=255)
                           cGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAgg                                                                                                               <  5:124409/139‑1 (MQ=255)
                               gggCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCgg                                                                                                            <  7:158176/138‑1 (MQ=255)
                                  cAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATAc                                                                                                        <  8:124453/139‑1 (MQ=255)
                                   aGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACg                                                                                                       <  7:427034/139‑1 (MQ=255)
                                     aaCGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACgct                                                                                                     >  4:15381/1‑139 (MQ=255)
                                     aaCGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACgct                                                                                                     >  4:268130/1‑139 (MQ=255)
                                       cGATGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTTTTCAGGAAGGCGGATACgctgc                                                                                                   >  6:45162/1‑139 (MQ=255)
                                          tGTTTGCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTAAGGAATGCGGATACGCTGCTa                                                                                                 >  2:27091/1‑138 (MQ=255)
                                               gCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTAtt                                                                                                                       <  6:109424/111‑1 (MQ=255)
                                               gCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTAtt                                                                                                                       >  5:109424/1‑111 (MQ=255)
                                               gCTTTCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAACGgcg                                                                                           >  8:381734/1‑139 (MQ=255)
                                                   tCGATGCCTGCGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCt                                                                                         <  7:147532/137‑1 (MQ=255)
                                                             cGTCATCATTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATctct                                                                             >  5:275531/1‑139 (MQ=255)
                                                                   catTTTGTCTTTGCTTACTTATCTCTCCTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGaa                                                                        >  4:262794/1‑138 (MQ=255)
                                                                       ttATCTTTGCTTACTTATCTCTCCTACAAAGCAGCGCTGTATACCCTGGTGGCTGTATTTGTTGCTGCAAGATTAATTGATTTTATTCAGGTAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACg                                                                   >  4:338585/1‑139 (MQ=255)
                                                                                cttacttaTATCTCATATAAAGAAGCCATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTATTCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAACACGACCTGAtt                                                           <  2:271870/138‑1 (MQ=255)
                                                                                              cTATAAAGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCtt                                            >  4:105656/1‑139 (MQ=255)
                                                                                                 taaaGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACg                                                                                                       <  5:102927/76‑1 (MQ=255)
                                                                                                 taaaGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACg                                                                                                       >  6:102927/2‑77 (MQ=255)
                                                                                                  aaaGAAGCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTg                                           >  4:358385/1‑136 (MQ=255)
                                                                                                   aagaagCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTgaag                                        >  7:137769/1‑138 (MQ=255)
                                                                                                      aatcGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATAc                                                                                                        >  2:399954/4‑71 (MQ=255)
                                                                                                      aagCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATAc                                                                                                        <  1:399954/71‑1 (MQ=255)
                                                                                                      aagCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAAt                                    >  1:299656/1‑139 (MQ=255)
                                                                                                      aagCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAAt                                    >  7:283046/1‑139 (MQ=255)
                                                                                                      aagCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAAt                                    >  2:261583/1‑139 (MQ=255)
                                                                                                        gCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATg                                   >  8:47271/1‑138 (MQ=255)
                                                                                                        gCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATg                                   >  5:416155/1‑138 (MQ=255)
                                                                                                        gCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATg                                   >  8:195773/1‑138 (MQ=255)
                                                                                                        gCGATGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATACTTGATGaaaag                                   >  3:23239/1‑136 (MQ=255)
                                                                                                           aTGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGCCCTGATTCAGAAAAAGATTCTTGAAGAAATGGacc                               >  8:379973/1‑137 (MQ=255)
                                                                                                           aTGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATGGAAc                               >  5:448103/1‑139 (MQ=255)
                                                                                                           aTGTATACGCTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATGGAAc                               >  8:385273/1‑139 (MQ=255)
                                                                                                                   gcTGGTAGCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGGGGCGTc                       <  8:137769/138‑1 (MQ=255)
                                                                                                                      ggtagatGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGGGGCGTCAc                     >  2:313918/7‑138 (MQ=255)
                                                                                                                      ggtagCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGa                                                                                                           >  4:321300/1‑52 (MQ=255)
                                                                                                                      ggtagCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGa                                                                                                           <  3:321300/52‑1 (MQ=255)
                                                                                                                      ggtagCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGGGGCGTcccc                    >  1:88757/1‑139 (MQ=255)
                                                                                                                      ggtagCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGGGGCGTCAc                     >  8:61466/1‑138 (MQ=255)
                                                                                                                       gtagCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGGGGCGTCACCa                   >  6:237624/4‑139 (MQ=255)
                                                                                                                       gtagCTGTATTTGTAGCGGCAAGATTAATCGATATTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTCAAGAAATGGAACGGGGCCTCACCa                   >  1:278668/4‑139 (MQ=255)
                                                                                                                        tagCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGGGGCGTCACCAt                  >  5:420035/3‑139 (MQ=255)
                                                                                                                        tagCTGTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGGGGCGTCACCAt                  >  3:427561/3‑139 (MQ=255)
                                                                                                                             gTATTTGTAGCGGCAATATTAAGCGATTTTATTCAGGTAGGCGGATAGGCTGCTAACGGCCCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGGGGCGTCACCATTTTGa             <  8:83617/139‑1 (MQ=255)
                                                                                                                             gTATTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGGGGCGTCACCATTTTg              >  1:391321/1‑138 (MQ=255)
                                                                                                                              tATTTGTAGCGGAAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGGGGCGTCACCATTTTGa             <  3:346229/138‑1 (MQ=255)
                                                                                                                               aTTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATGGATCGGGGCGTCACCATTTTGa             >  5:89200/1‑137 (MQ=255)
                                                                                                                               aTTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGGGGCGTCACCATTTTGa             >  2:56706/1‑137 (MQ=255)
                                                                                                                               aTTTGTAGCGGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGGGGCGTCACCATTTTGa             >  8:341977/1‑137 (MQ=255)
                                                                                                                                        tGCAAGATTAATCGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATCATCTCTTCGAAAAACGACCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGGGGCGTCACCATTTTGAAAGGACAAGGt  >  8:318544/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                          
GTTTGGTCAATAAGTATTTTGGGATTCCGATGGGCAGAACGATGTTTGCTTTCGATGCCTGCGTCATCATTTTATCTTTGCTTACTTATCTCTCCTACAAAGAAGCGATGTATACCCTGGTGGCTGTATTTGTAGCTGCAAGATTAATTGATTTTATTCAGGAAGGCGGATACGCTGCTAAAGGCGCTACGATTATCTCTTCGAAAAACGATCTGATTCAGAAAAAGATTCTTGAAGAAATGGAACGAGGCGTCACCATTTTGAAAGGACAGGGT  >  CP006881/2178900‑2179174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑