Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,137,474	T→C	E135G (GAG→GGG)	U712_10680 ←	Xanthine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,137,474	0	T	C	100.0%	167.6 / NA	52	E135G (GAG→GGG)	U712_10680	Xanthine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/26);  total (26/26)

TGAATTCTTGCCAAAGATTCCACTCGGTAGCCCAGTTTTACAAGTTCATCTCTTCCCGGCTGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCTCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGCACATGATCCTGATCCGACAGGTGGGTCCCAGACACTGCGATTTGGCTTTCTGTTTGCTTCGTAAAGGAATAAACAGACGCTGTCAGCAAGT  >  CP006881/2137342‑2137609                                                                                                                                     |                                                                                                                                        tGAATTCTTGCCAAAGATTCCACTCGGTAGCCCAGTTTTACAAGTTCATCTCTTCCCGGCTGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTgcg                                                                                                                                   >  4:429424/1‑139 (MQ=255) tGAATTCTTGCCAAAGATTCCACTCGGTAGCCCAGTTTTACAAGTTCATCTCTTCCCGGCTGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTgcg                                                                                                                                   >  4:389856/1‑139 (MQ=255) tGAATTCTTGCCAAAGATTCCACTCGGTAGCCCAGGTTTACAAGTTCATCTCTTCCCGGCTGAAAGGACTTTTCAATAACAAAGCCGATACCCGCAATAGAACCTACCGCTTGCTTCACAATCGACACACGCCCGTgcg                                                                                                                                   >  1:280510/1‑139 (MQ=255)   aaTTCTTGCCAAAGATTCCACTCGGTAGCCCAGTTTTACAAGTTCATCTCTTCCCGGCTGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTgcg                                                                                                                                   <  3:155391/137‑1 (MQ=255)   aaTTCTTGCCAAAGATTCCACTCGGTAGCCCAGTTTTACAAGTTCATCTCTTCCCGGCTGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCt                                                                                                                                 >  6:161307/1‑139 (MQ=255)   aaTTCTTGCCAAAGATTCCACTCGGTAGCCCAGTTTTACAAGTTCATCTCTTCCCGGCTGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCt                                                                                                                                 >  1:72390/1‑139 (MQ=255)   aaTTCTTGCCAAAGATTCCACTCGGTAGCCCAGTTTTACAAGTTCATCTCTTCCCGGCTGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCt                                                                                                                                 >  7:217296/1‑139 (MQ=255)   aaTTATTGCCAAAGATTCCACTCGGTAGCCCAGTTTTACAAGTTCATCTCTTCCCGGCTGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTgcg                                                                                                                                   <  1:358530/137‑1 (MQ=255)          gCCAAAGATTCCACTCGGTAGCCCAGTTTTACAAGTTCATCTCTTCCCGGCTGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTgcg                                                                                                                                   >  6:333278/1‑130 (MQ=255)          gCCAAAGATTCCACTCGGTAGCCCAGTTTTACAAGTTCATCTCTTCCCGGCTGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTgcg                                                                                                                                   <  5:333278/130‑1 (MQ=255)             aaaGATTCCACTCGGTAGCCCAGTTTTACAAGTTCATCTCTTCCCGGCTGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCAt                                                                                                                       <  7:288610/139‑1 (MQ=255)                     cACTCGGTAGCCCAGTTTTACAAGTTCATCTCTTCCCGGCTGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCaaa                                                                                                               <  3:207556/139‑1 (MQ=255)                          ggTAGCCCAGTTTTACAAGTTCATCTCTTCCCGGCTGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAat                                                                                                           >  5:139064/1‑138 (MQ=255)                           gTAGCCCAGTTTTACAAGTTCATCTCTTCCCGGCTGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAat                                                                                                           >  5:196471/1‑137 (MQ=255)                           gTAGCCCAGTTTTACAAGTTCATCTCTTCCCGGCTGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAat                                                                                                           <  6:196471/137‑1 (MQ=255)                              gCCCAGTTTTACAAGTTCATCTCTTCCCGGCTGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCg                                                                                                      <  8:217296/139‑1 (MQ=255)                                  aGTTTTAAAAGTTCATCTCTTCCCGGCTGAAATGACTATTCAATAACAATGCCGATTACCGCAATAGAAGCTCCCGCATGCTTCACAATCTACACAAGCCCCTGCGCTGCATGTCCATTAGCCAAAAAATCAACGaaa                                                                                                   >  1:438939/1‑136 (MQ=255)                                      ttACAAGTCCATCTCTTCCCGGCGGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCa                                                                                               <  1:180599/138‑1 (MQ=255)                                       tACAAGTTCATCTCTTCCCGGCTGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAg                                                                                              <  3:429424/138‑1 (MQ=255)                                          aaGTTCATCTCTTCCCGGCTGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGcaca                                                                                          <  5:54833/139‑1 (MQ=255)                                          aaGTTCATCTCTTCCCGGCTGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGcaca                                                                                          <  7:159094/139‑1 (MQ=255)                                          aaGTTCATCTCTTCCCGGCTGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGcaca                                                                                          <  6:139064/139‑1 (MQ=255)                                          aaGTTCATCTCTTCCCGGCTGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGcaca                                                                                          <  5:161307/139‑1 (MQ=255)                                          aaGTTCATCTCTTCCCGGCTGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGcaca                                                                                          <  7:415212/139‑1 (MQ=255)                                              tCATCTCTTCCCGGCTGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGGCTGTCCATTTGCCAAAAAATCATCGATAATCAGCACAtgat                                                                                      >  3:2216/1‑139 (MQ=255)                                               cATCTCTTCCCGGCTGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGCACAtgatc                                                                                     >  6:350868/1‑139 (MQ=255)                                                aTCTCTTCCCGGCTGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGCACAtgatc                                                                                     <  1:234373/138‑1 (MQ=255)                                                       cccGGCTGCAAAGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGCACAtgatcgtgatcct                                                                             >  2:30435/1‑138 (MQ=255)                                                             tGAAATGACTTTTAAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGCACATGATCCTGATCCGACAGGt                                                                       <  2:165021/139‑1 (MQ=255)                                                               aaaTGACTTTTCAATAACAATGCCGCTTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCATGTCCATTTGCCAAAAAATCATCGATAATCAGCACATGATCCTGATACGACAGGTgg                                                                     >  8:322353/1‑139 (MQ=255)                                                               aaaTGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGCACATGATCCTGATCCGACAGGt                                                                       <  2:234867/137‑1 (MQ=255)                                                                attGAGTTTTTAATAACAATGCCGATTCCCAATATAGTATCTCACGTTTGCTTCACATTCTACACTAGCCCGTGGGATTTCTGTTGATTTGTCATAAAATCATCTATTATAAGAACATTATCCTGATCCTTCAGGTgg                                                                     <  8:385808/136‑1 (MQ=255)                                                                           aaTAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGGGCGCTGCCTGTCCATTTTACAAAAAATCATCGATAATCCGCACATGATCCTGATCCGACAGGTGGGGCCCAGACACt                                                         <  7:143773/139‑1 (MQ=255)                                                                                   tGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGCACATGATCCTGATCCGACAGGTGGGTCCCAGACACTGCGATTTg                                                 >  3:12294/1‑139 (MQ=255)                                                                                     ccGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGACAAAAAATCATCGATATTCAGCACATACTCCTGATCCGACAGGTGGGTCCCAGCCACTGCGATTTGGc                                               >  4:56847/1‑139 (MQ=255)                                                                                        aTTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGCACATGATCCTGATCCGACAGGTgg                                                                     >  7:274720/1‑114 (MQ=255)                                                                                        aTTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGCACATGATCCTGATCCGACAGGTgg                                                                     <  8:274720/114‑1 (MQ=255)                                                                                          tCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGCACATGATCCTGATCCGACAGGTGGGTCCCAGACACTGCGATTTGGCTTTCt                                          >  8:426301/1‑139 (MQ=255)                                                                                             cGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGCACATGATCCTGATCCGACAGGTGGGTCCCAGACACTGCGATTTGGCTTTCTGtt                                       >  2:98199/1‑139 (MQ=255)                                                                                             cGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGCACATGATCCTGATCCGACAGGTGGGTCCCAGACACAGCGATTTGGCTTTCTGtt                                       >  4:167794/1‑139 (MQ=255)                                                                                                      aGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGCACATGATCCTGATCCGACAGGTGGGTCCCAGACACTGCGATTTGGCTTTCTGTTTGCTTCGt                               >  5:331129/1‑138 (MQ=255)                                                                                                      aGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGCACATGATCCTGATCCGACAGGTGGGTCCCAGACACTGCGATTTGGCTTTCTGTTTGCTTCGTa                              <  5:430263/139‑1 (MQ=255)                                                                                                      aGCTCCCGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGCACATGATCCTGATCCGACAGGTGGGTCCCAGACACTGCGATTTGGCTTTCTGTTTGCTTCGTa                              <  1:229252/139‑1 (MQ=255)                                                                                                      aGCTCCCGCTTGCTTCACAATCGACACAAGCCCGGGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGCACATGATCCTGATCCGACAGGTGGGTCCCAGACACTGCGATTTGGCTTTCTGTTTGCTTCGTa                              <  5:96629/139‑1 (MQ=255)                                                                                                      aGCTCCCGCTTGCTTCACAATCGACACAACCCCGAGCGCTGCCTGTCCATTTGCCAAAAAATAATCGATAATCAGCAGATGATCCTGATCCGACAGGGGGGTCCCAGACACTGCGAATTGGCTTTCTGTTTGCTTCGTa                              <  5:56234/139‑1 (MQ=255)                                                                                                          cccGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGCACATGATCCTGATCCGACAGGTGGGTCCCAGACACTGCGATTTGGCTTTCTGTTTGCTTCGTAAAg                           >  7:350498/1‑138 (MQ=255)                                                                                                          cccGCTTGCTTCACAATCGACACAAGCCCGTGCGCTGACTGTCCATTTGCCAAAAAATCATCGATAATCAGAACATGATCCTGATCCGACAGGTGGGTCCCAGACACTGCGATTTGGCTTTCTGTTTGCTTCGTAAAg                           >  2:404164/1‑138 (MQ=255)                                                                                                                 gcttCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAGAAAATCATCGATAATCAGCACATGATCCTGATCCGACAGGTGGGTCCCAGACACTGCGATTTGGCTTTCTGTTTGCTTCGTAAAGGAATaaa                    <  4:212165/138‑1 (MQ=255)                                                                                                                  cttCACAATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGCACATGATCCTGATCCGACAGGTGGGTCCCAGACACTGCGATTTGGCTTTCTGTTTGCTTCGTAAAGGAATaaa                    >  7:71348/1‑137 (MQ=255)                                                                                                                     cacaATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGCACATGATCCTGATCCGACAGGTGGGTCCCAGACACTGCGATTTGGCTTTCTGTTTGCTTCGTAAAGGAATAAACAGAc               >  7:36339/1‑139 (MQ=255)                                                                                                                      acaATCGACACAAGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGCACATGATCCTGATCCGACAGGTGGGTCCCAGACACTGCGATTTGGCTTTCTGTTTGCTTCGTAAAGGAATAAACAGACg              <  1:22376/139‑1 (MQ=255)                                                                                                                                  aGCCCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGCACATGATCCTGATCCGACAGGTGGGTCCCAGACACTGCGATTTGGCTTTCTGTTTGCTTCGTAAAGGAATAAACAGACGCTGTCAGCAAGt  >  1:64093/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                        TGAATTCTTGCCAAAGATTCCACTCGGTAGCCCAGTTTTACAAGTTCATCTCTTCCCGGCTGAAATGACTTTTCAATAACAATGCCGATTCCCGCAATAGAAGCTCCCGCTTGCTTCACAATCGACACAAGCTCGTGCGCTGCCTGTCCATTTGCCAAAAAATCATCGATAATCAGCACATGATCCTGATCCGACAGGTGGGTCCCAGACACTGCGATTTGGCTTTCTGTTTGCTTCGTAAAGGAATAAACAGACGCTGTCAGCAAGT  >  CP006881/2137342‑2137609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑