| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,179,327 | C→T | D282D (GAC→GAT) | U712_10905 → | UPF0750 membrane protein ypjC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,179,327 | 0 | C | T | 98.4% | 172.5 / NA | 61 | D282D (GAC→GAT) | U712_10905 | UPF0750 membrane protein ypjC |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (49/11); minor base G (0/1); total (49/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATAGATGTCCTTTATTGTGTTGTGCCAAAAAACGAATTGGTGATGCTGAAAAGTGTGATTAACTCCGTTGATCCTCATGCCTTTGTGGCGGTAAGCGATGTCCACGACGTGCTTGGAGAAGGATTTACGCTTGACGAAAATAAAAATCCGCTTCCGCGCTGACATTTGATAGAAAAAAGGCGGCAG‑CAGCCGCCCC‑‑‑T‑‑‑‑C‑T‑‑‑TATTTAGGGTAGTCCCGTTTTTTCAGCTTGTTTTTCTCCGCTTTGTACTTTCTGTAGCCGACA > CP006881/2179193‑2179454 | ataGATGTACTTTATTGTGTGGTGCCGAAAAATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGaa > 3:7073/1‑138 (MQ=255)ataGATGTACTTTATTGTGTGGTGCCGAAAAATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGaa > 7:307731/1‑138 (MQ=255) tacTTTATTGTGTGGTGCCGAAAAATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATaaaaa > 1:90257/3‑139 (MQ=255) tacTTTATTGTGTGGTGCCGAAAAATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATaaaaa > 7:24574/3‑139 (MQ=255) tacTTTATTGTGTGGTGCCGAAAAATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATaaaaa > 1:691/3‑139 (MQ=255) tacTTTATTGTGTGGTGCCGAAAAATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATaaaaa > 4:72924/3‑139 (MQ=255) tacTTTATTGTGTGGTGCCGAAAAATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATaaaaa > 1:427080/3‑139 (MQ=255) tacTTTATTGTGTGGTGCCGAAAAATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATaaaaa > 1:297913/3‑139 (MQ=255) cTTTATTGTGTGGTGCCGAAAAATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATc > 7:81891/1‑139 (MQ=255) cTTTATTGTGTGGTGCCGAAAAATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATc > 5:135663/1‑139 (MQ=255) cTTTATTGTGTGGTGCCGAAAAATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATc > 5:415305/1‑139 (MQ=255) cTTTATTGTGTGGTGCCGAAAAATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATc > 5:273041/1‑139 (MQ=255) tATTGTGTGGTGCCGAAACATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGAAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATcctc > 5:125770/1‑137 (MQ=255) tATTGTGTGGTGCCGAAAAATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGc > 1:314907/1‑139 (MQ=255) tATTGTGTGGTGCCGAAAAATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGc > 5:403779/1‑139 (MQ=255) ttgtttggtgCCGAAAAATGAATTGGTGTTGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGTTGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCtt < 7:195773/139‑1 (MQ=255) tgtggtgCCGAAAAATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCcg > 3:242753/1‑139 (MQ=255) tgtggtgCCGAAAAATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCcg > 6:106700/1‑139 (MQ=255) gtggtgCCGAAAAATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCcgc > 5:197308/4‑139 (MQ=255) tggtgCCGAAAAATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCcgcg > 3:413193/3‑139 (MQ=255) tggtgCCGAAAAATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCcgcg > 7:320611/3‑139 (MQ=255) tggtgCCGAAAAATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCcgcg > 8:334409/3‑139 (MQ=255) tggtgCCGAAAAATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCcgcg > 1:210690/3‑139 (MQ=255) gtgCCGAAAAATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCt < 5:106700/139‑1 (MQ=255) tgCCGAAAAATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCt > 1:309999/1‑138 (MQ=255) tgCCGAAAAATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTg > 8:419592/1‑139 (MQ=255) tgCCGAAAAATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGATCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCt > 6:129171/1‑138 (MQ=255) gCCGAAAAATGAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGa > 5:65399/1‑139 (MQ=255) tgAATTGGTGATGCTGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACAtgtaa > 8:91238/2‑133 (MQ=255) tGAAAAGCGTGATTAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAggcggct > 4:269919/1‑138 (MQ=255) ttAACTCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGCCCGCCGcccc > 2:353829/1‑139 (MQ=255) aaCTCCATTGATCCCCATGCCTTTGTAGCGGTACGCGATGTTCACGATGTGCTCGGAGAAGTATTTACGCTTTATGAAAAAAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCc‑‑‑t < 3:72924/138‑1 (MQ=255) tCCATTGATCCCCATGCCTTTGTAGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCc‑‑‑t‑‑‑‑catc > 8:148931/1‑136 (MQ=255) tgtaGCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCC‑‑‑TCATCC‑T‑‑‑TATTTAGGGTAGTccctt > 7:421086/1‑137 (MQ=255) tagCGGTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCC‑‑‑TCATCC‑T‑‑‑TATTTAGGGTAGTCCCtttt > 5:139205/3‑139 (MQ=255) gTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTATTAGAAAAAAGGCGGCTGACCGCCGCCCC‑‑‑TACTCC‑T‑‑‑TATTTAGGGTAGTCCGTtttttt > 6:385839/1‑137 (MQ=255) gTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAACAGGCGGCTGACCGCCGCCCCGCATC‑‑‑C‑T‑‑‑TATTTAGGGTAGTGCCTtttttt > 1:281487/1‑137 (MQ=255) gTAAGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGATTCCGCGATGACATGTAATAGAAAAACGGCGGCTGACCGCCGCCCC‑‑‑TCATCC‑T‑‑‑TATTTAGGGTAGTCCCTtttttt > 7:369165/1‑137 (MQ=255) gTAAGCGATGTTCACGATGTGATCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAATTCGGCTGCCCGCCGCCCC‑‑‑TCATCC‑T‑‑‑TATTTAGGGTAGTCCCTtttttt > 5:208171/1‑137 (MQ=255) aGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGCCCGCCGCCCC‑‑‑TCATCC‑T‑‑‑GATTTAGGGTAGTCCCTTTTTTTAAGCt > 6:348531/1‑139 (MQ=255) aGCGATGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCC‑‑‑T‑‑‑‑CATCCGTATTTAGGGTAGTCCCTtttttttggcg > 1:274187/1‑134 (MQ=255) tGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAACAAGGCGGCTGCCAGCCGCCCCGCATC‑‑‑C‑T‑‑‑TATTTAGGGTAGTCCCTTTTTTTCAGCTTGttt > 3:289088/1‑139 (MQ=255) tGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCC‑‑‑TCATCC‑T‑‑‑TATTTAGGGTAGTCCCTTTTTTTCAGCTTGttt > 6:303356/1‑139 (MQ=255) tGTTCACGATGTGCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCC‑‑‑TCATCC‑T‑‑‑TATTTAGGGTAGTCCCTTTTTTTAAGCTTGttt > 6:303360/1‑139 (MQ=255) acgatgTGCTCGGAGAAGGATTTACGCTTGAGGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCC‑‑‑TCATCC‑T‑‑‑TATTTAGGGTAGTCCCTTTTTTTCAGCTTGttttttt < 7:61466/138‑3 (MQ=255) gCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCC‑‑‑TCATCC‑T‑‑‑TATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGcct > 2:45648/1‑135 (MQ=255) gCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCC‑‑‑TCATCC‑T‑‑‑TATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGcct < 1:45648/137‑3 (MQ=255) gCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCC‑‑‑TCATCC‑T‑‑‑TATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTg > 8:105686/1‑139 (MQ=255) tcggAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCC‑‑‑TCATCC‑T‑‑‑TATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTa > 2:110579/3‑139 (MQ=255) ggAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGAAATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCC‑‑‑TCATCC‑T‑‑‑TATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACt < 6:415305/139‑1 (MQ=255) gagaAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCC‑‑‑TCATCC‑T‑‑‑TATTTAGGGTAGTCCCGTTTTTTGAGCTTGTTTTTTTCCGGCTTGTACtt > 6:327739/1‑139 (MQ=255) aGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCAGACCGCCGCCCCAAATC‑‑‑C‑T‑‑‑TATTTATGGTAGTCCCGTTTTTTTAGCTTGTTTTTTTGAGCATTGTACattatt > 4:251429/1‑136 (MQ=255) taTTTCCGCTTCATTAAAAAAAAAACCCGCTTCCGCGCTGACATGTAATAGAAAAACGGCGGCTGACCGCCGCCCC‑‑‑TCATCC‑T‑‑‑TATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTa < 8:177603/138‑1 (MQ=255) gATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGCCCGCCGCCCC‑‑‑TCATCC‑T‑‑‑TATTTAGGGTAGTCCCTTTTTTTCAGCTGGTTTTTTTACTCATTGTACTTTCTGt > 8:197539/1‑138 (MQ=255) ttACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCC‑‑‑TCATCC‑T‑‑‑TATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTaac < 4:413193/138‑3 (MQ=255) ttACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCC‑‑‑TCATCC‑T‑‑‑TATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAAcc > 6:318750/1‑139 (MQ=255) ttACGATTGATGAAAATAAAAACCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCC‑‑‑TCATCC‑T‑‑‑TATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTaac < 6:135663/138‑3 (MQ=255) tACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCC‑‑‑TCATCC‑T‑‑‑TATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAAcc > 2:321057/1‑138 (MQ=255) gCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCC‑‑‑TCATCC‑T‑‑‑TATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACa < 5:84532/139‑1 (MQ=255) gCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCC‑‑‑TCATCC‑T‑‑‑TATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACa < 8:307731/139‑1 (MQ=255) gCTTGATGAAAAAAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCC‑‑‑TCATCC‑T‑‑‑TATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACa < 2:314907/139‑1 (MQ=255) | ATAGATGTCCTTTATTGTGTTGTGCCAAAAAACGAATTGGTGATGCTGAAAAGTGTGATTAACTCCGTTGATCCTCATGCCTTTGTGGCGGTAAGCGATGTCCACGACGTGCTTGGAGAAGGATTTACGCTTGACGAAAATAAAAATCCGCTTCCGCGCTGACATTTGATAGAAAAAAGGCGGCAG‑CAGCCGCCCC‑‑‑T‑‑‑‑C‑T‑‑‑TATTTAGGGTAGTCCCGTTTTTTCAGCTTGTTTTTCTCCGCTTTGTACTTTCTGTAGCCGACA > CP006881/2179193‑2179454 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |