Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,179,433 T→C K251K (AAA→AAG)  U712_10910 ← Uncharacterized protein ypjB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,179,4330TC96.5% 173.6 / NA 59K251K (AAA→AAG) U712_10910Uncharacterized protein ypjB
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base C (19/36);  minor base A (2/0);  total (21/38)
Fisher's exact test for biased strand distribution p-value = 1.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTGGAGAAGGATTTACGCTTGACGAAAATAAAAATCCGCTTCCGCGCTGACATTTGATAGAAAAAAGGCGGCAG‑CA‑GCCGCCCC‑‑‑T‑‑‑‑CTTATTTAGGGTAGTCCCGTTTTTTCAGCTTGTTTTTCTCCGCTTTGTACTTTCTGTAGCCGACATAGGTCAGAGCTGTAATGATGATACTGCCGGTTGTGATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAAAACATTTTTCAGATCATGCTGAA  >  CP006881/2179303‑2179566
                                                                                                                                           |                                                                                                                                     
gCTCGGAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTg                                                                                                                                    >  8:105686/1‑139 (MQ=255)
  tcggAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTa                                                                                                                                  >  2:110579/3‑139 (MQ=255)
    ggAGAAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGAAATGTAATAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACt                                                                                                                                <  6:415305/139‑1 (MQ=255)
     gagaAGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCGTTTTTTGAGCTTGTTTTTTTCCGGCTTGTACtt                                                                                                                               >  6:327739/1‑139 (MQ=255)
         aGGATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCAGACC‑GCCGCCCCAAATC‑‑‑CTTATTTATGGTAGTCCCGTTTTTTTAGCTTGTTTTTTTGAGCATTGTACattatt                                                                                                                           >  4:251429/1‑136 (MQ=255)
           taTTTCCGCTTCATTAAAAAAAAAACCCGCTTCCGCGCTGACATGTAATAGAAAAACGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTa                                                                                                                         <  8:177603/138‑1 (MQ=255)
           gATTTACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGCCC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTGGTTTTTTTACTCATTGTACTTTCTGt                                                                                                                          >  8:197539/1‑138 (MQ=255)
              ttACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTaac                                                                                                                       <  4:413193/138‑3 (MQ=255)
              ttACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAAcc                                                                                                                      >  6:318750/1‑139 (MQ=255)
              ttACGATTGATGAAAATAAAAACCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTaac                                                                                                                       <  6:135663/138‑3 (MQ=255)
               tACGCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAAcc                                                                                                                      >  2:321057/1‑138 (MQ=255)
                  gCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACa                                                                                                                  <  5:84532/139‑1 (MQ=255)
                  gCTTGATGAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACa                                                                                                                  <  8:307731/139‑1 (MQ=255)
                  gCTTGATGAAAAAAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACa                                                                                                                  <  2:314907/139‑1 (MQ=255)
                      gatgAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAgg                                                                                                              >  4:338108/4‑139 (MQ=255)
                        tgAAAATAAAATACCGATTACGCGCTTTCATGAAATAGAAAAAAGGCGGCTG‑AACGCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGt                                                                                                             <  6:199138/137‑1 (MQ=255)
                        tgAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGt                                                                                                             <  1:321057/137‑1 (MQ=255)
                        tgAAAATAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGt                                                                                                             <  5:318750/137‑1 (MQ=255)
                          aaaaaaaaaaTCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAag                                                                                                          <  2:691/139‑1 (MQ=255)
                            aaTAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAagag                                                                                                        <  8:249270/139‑1 (MQ=255)
                            aaTAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAagag                                                                                                        <  8:5353/139‑1 (MQ=255)
                            aaTAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAagag                                                                                                        <  7:91238/139‑1 (MQ=255)
                                aaaaTCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGt                                                                                                    <  3:319030/139‑1 (MQ=255)
                                aaaaTCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGt                                                                                                    <  7:105686/139‑1 (MQ=255)
                                     ccGCTTCCGCGCGGACATGTAAAAGAAAAAAGGCGGCTGACC‑GACGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAatga                                                                                               <  8:81891/139‑1 (MQ=255)
                                        cTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTa                                                                                                                                  <  7:351912/101‑1 (MQ=255)
                                        cTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTa                                                                                                                                  >  8:351912/1‑101 (MQ=255)
                                           ccGCGCTGACATGTAATAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATgatgat                                                                                           <  5:205428/137‑1 (MQ=255)
                                            cgcgCTGACATTTAATAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACt                                                                                        <  5:129171/139‑1 (MQ=255)
                                              cgcTTACATGTAAAAGAAAAAACGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGc                                                                                      <  1:345318/139‑1 (MQ=255)
                                              cgcTGACATGTAATAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGc                                                                                      <  8:376607/139‑1 (MQ=255)
                                                     atgtaaTAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATGCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTtgt                                                                               <  6:197308/134‑1 (MQ=255)
                                                          aTAGAAAAAAGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGtaatga                                                                          <  1:110579/139‑1 (MQ=255)
                                                              aaaaaaGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAag                                                                                                          >  1:24129/1‑103 (MQ=255)
                                                              aaaaaaGGCGGCTGACC‑GCCGCCCC‑‑‑TCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAag                                                                                                          <  2:24129/103‑1 (MQ=255)
                                                                                      cc‑‑‑T‑‑‑‑CTTATTTAGGGTAGTCCCGTTTTTTCAGCTTGTTTTTCTCCGCTTTGAACTTTCTGTAGCCGACATAGGGCAGAGCTGTAATGATGATACTGCCGGTTGTGATGATCACCCAGAGTAAAGACGGATCGGCatcatc                                           <  3:338585/139‑1 (MQ=255)
                                                                                                  tATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCAtcctcct                                      >  8:250836/1‑139 (MQ=255)
                                                                                                         ggTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCt                               >  6:333539/1‑139 (MQ=255)
                                                                                                         ggTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCt                               >  4:91417/1‑139 (MQ=255)
                                                                                                         ggTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCt                               >  7:33672/1‑139 (MQ=255)
                                                                                                             gTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCtctc                            >  3:105017/1‑138 (MQ=255)
                                                                                                             gTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTc                            <  8:24574/138‑1 (MQ=255)
                                                                                                              tCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACa                                                                                                                  <  3:48732/51‑1 (MQ=255)
                                                                                                              tCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCgg                                                                                   >  7:209672/1‑82 (MQ=255)
                                                                                                              tCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCgg                                                                                   <  8:209672/82‑1 (MQ=255)
                                                                                                              tCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCa                           >  3:349851/1‑138 (MQ=255)
                                                                                                              tCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCa                           >  6:73420/1‑138 (MQ=255)
                                                                                                              tCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCa                           >  5:321274/1‑138 (MQ=255)
                                                                                                                 cttttattCATCTTGTTTTTATCCGCCTTGTTCTTTCTGAACCCGAAATAGGTAAGACCAGTAATGATGATACTGCCGGTTGTATTGATCACCAAGATTAAAGACGGATCGGCATCATCCTCCTCCACCGAGTCaaaca                       <  2:286956/137‑3 (MQ=255)
                                                                                                                    tttttCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAAcaca                     <  5:361408/138‑1 (MQ=255)
                                                                                                                                ttttttTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCAt        >  3:381844/1‑139 (MQ=255)
                                                                                                                                ttttttTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAAAACATTTTTCAGGTCAt        >  5:251189/1‑139 (MQ=255)
                                                                                                                                  ttttgccGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTATTGATCACCCAGAGTACCGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATg       <  5:348531/133‑1 (MQ=255)
                                                                                                                                  ttttTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCATTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATg       <  8:34112/138‑1 (MQ=255)
                                                                                                                                  cttttcCGCCTTGTCCTTTCTCTAACCCACATACGCACGAGCAGTAATGATGATACTGCCGGTGGTAATGAACACCCAGACAAACCACGGATCGGCATCATCCTCCTCCACCCTGCCAAACACATTTTTCAGGTCATg       <  4:349851/134‑1 (MQ=255)
                                                                                                                                     ttcCGCGTTGTACTTTCGGTAACCGACTTAGGTAAGAGCAGTATTGATGATACTGCCGGTTGTAATTCTCACCCAGAGTAATGACGGATCGGCATGATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTg    <  7:221497/137‑1 (MQ=255)
                                                                                                                                     ttcCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGTTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTg    <  3:153208/137‑1 (MQ=255)
                                                                                                                                       ccGCCTTGTACTTTCTTAAACCGAAATTGGTTAGAGCAGTAATGTTGATACTGCCGGTTTTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTgaa  <  3:297912/138‑1 (MQ=255)
                                                                                                                                       ccGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTgaa  <  5:35846/138‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                     
GCTTGGAGAAGGATTTACGCTTGACGAAAATAAAAATCCGCTTCCGCGCTGACATTTGATAGAAAAAAGGCGGCAG‑CA‑GCCGCCCC‑‑‑T‑‑‑‑CTTATTTAGGGTAGTCCCGTTTTTTCAGCTTGTTTTTCTCCGCTTTGTACTTTCTGTAGCCGACATAGGTCAGAGCTGTAATGATGATACTGCCGGTTGTGATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAAAACATTTTTCAGATCATGCTGAA  >  CP006881/2179303‑2179566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑