Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,179,460 C→A L242L (CTG→CTT)  U712_10910 ← Uncharacterized protein ypjB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,179,4600CA97.6% 122.6 / ‑3.7 42L242L (CTG→CTT) U712_10910Uncharacterized protein ypjB
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (14/26);  total (14/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

AAAATAAAAATCCGCTTCCGCGCTGACATTTGATAGAAAAAAGGCGGCAG‑CAGCCGCCCCT‑‑‑‑CTTATTTAGGGTAGTCCCGTTTTTTCAGCTTGTTTTTCTCCGCTTTGTACTTTCTGTAGCCGACATAGGTCAGAGCTGTAATGATGATACTGCCGGTTGTGATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAAAACATTTTTCAGATCATGCTGAAGAAGAGAAAGCCGTTCAAGCTTTG  >  CP006881/2179329‑2179590
                                                                                                                                        |                                                                                                                                  
aaaaaaaaaaTCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCCTCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAag                                                                                                                                  <  2:691/139‑1 (MQ=255)
  aaTAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCCTCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAagag                                                                                                                                <  8:5353/139‑1 (MQ=255)
  aaTAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCCTCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAagag                                                                                                                                <  8:249270/139‑1 (MQ=255)
  aaTAAAAATCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCCTCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAagag                                                                                                                                <  7:91238/139‑1 (MQ=255)
      aaaaTCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCCTCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGt                                                                                                                            <  7:105686/139‑1 (MQ=255)
      aaaaTCCGCTTCCGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCCTCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGt                                                                                                                            <  3:319030/139‑1 (MQ=255)
           ccGCTTCCGCGCGGACATGTAAAAGAAAAAAGGCGGCTGACCGACGCCCCTCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAatga                                                                                                                       <  8:81891/139‑1 (MQ=255)
                 ccGCGCTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCCTCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATgatgat                                                                                                                   <  5:205428/137‑1 (MQ=255)
                  cgcgCTGACATTTAATAGAAAAAAGGCGGCTGACCGCCGCCCCTCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACt                                                                                                                <  5:129171/139‑1 (MQ=255)
                    cgcTTACATGTAAAAGAAAAAACGCGGCTGACCGCCGCCCCTCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGc                                                                                                              <  1:345318/139‑1 (MQ=255)
                    cgcTGACATGTAATAGAAAAAAGGCGGCTGACCGCCGCCCCTCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGc                                                                                                              <  8:376607/139‑1 (MQ=255)
                           atgtaaTAGAAAAAAGGCGGCTGACCGCCGCCCCTCATGCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTtgt                                                                                                       <  6:197308/134‑1 (MQ=255)
                                aTAGAAAAAAGGCGGCTGACCGCCGCCCCTCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGtaatga                                                                                                  <  1:110579/139‑1 (MQ=255)
                                    aaaaaaGGCGGCTGACCGCCGCCCCTCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAag                                                                                                                                  >  1:24129/1‑103 (MQ=255)
                                    aaaaaaGGCGGCTGACCGCCGCCCCTCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAag                                                                                                                                  <  2:24129/103‑1 (MQ=255)
                                                           ccT‑‑‑‑CTTATTTAGGGTAGTCCCGTTTTTTCAGCTTGTTTTTCTCCGCTTTGAACTTTCTGTAGCCGACATAGGGCAGAGCTGTAATGATGATACTGCCGGTTGTGATGATCACCCAGAGTAAAGACGGATCGGCatcatc                                                                   <  3:338585/139‑1 (MQ=255)
                                                                    tATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCAtcctcct                                                              >  8:250836/1‑139 (MQ=255)
                                                                           ggTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCt                                                       >  4:91417/1‑139 (MQ=255)
                                                                           ggTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCt                                                       >  7:33672/1‑139 (MQ=255)
                                                                           ggTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCt                                                       >  6:333539/1‑139 (MQ=255)
                                                                               gTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCtctc                                                    >  3:105017/1‑138 (MQ=255)
                                                                               gTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTc                                                    <  8:24574/138‑1 (MQ=255)
                                                                                tCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCgg                                                                                                           >  7:209672/1‑82 (MQ=255)
                                                                                tCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCgg                                                                                                           <  8:209672/82‑1 (MQ=255)
                                                                                tCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCa                                                   >  5:321274/1‑138 (MQ=255)
                                                                                tCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCa                                                   >  6:73420/1‑138 (MQ=255)
                                                                                tCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCa                                                   >  3:349851/1‑138 (MQ=255)
                                                                                   cttttattCATCTTGTTTTTATCCGCCTTGTTCTTTCTGAACCCGAAATAGGTAAGACCAGTAATGATGATACTGCCGGTTGTATTGATCACCAAGATTAAAGACGGATCGGCATCATCCTCCTCCACCGAGTCaaaca                                               <  2:286956/137‑3 (MQ=255)
                                                                                      tttttCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAAcaca                                             <  5:361408/138‑1 (MQ=255)
                                                                                                  ttttttTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCAt                                >  3:381844/1‑139 (MQ=255)
                                                                                                  ttttttTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAAAACATTTTTCAGGTCAt                                >  5:251189/1‑139 (MQ=255)
                                                                                                    ttttgccGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTATTGATCACCCAGAGTACCGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATg                               <  5:348531/133‑1 (MQ=255)
                                                                                                    ttttTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCATTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATg                               <  8:34112/138‑1 (MQ=255)
                                                                                                    cttttcCGCCTTGTCCTTTCTCTAACCCACATACGCACGAGCAGTAATGATGATACTGCCGGTGGTAATGAACACCCAGACAAACCACGGATCGGCATCATCCTCCTCCACCCTGCCAAACACATTTTTCAGGTCATg                               <  4:349851/134‑1 (MQ=255)
                                                                                                       ttcCGCGTTGTACTTTCGGTAACCGACTTAGGTAAGAGCAGTATTGATGATACTGCCGGTTGTAATTCTCACCCAGAGTAATGACGGATCGGCATGATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTg                            <  7:221497/137‑1 (MQ=255)
                                                                                                       ttcCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGTTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTg                            <  3:153208/137‑1 (MQ=255)
                                                                                                         cggccttGTACTTGCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTgaa                          <  3:131981/133‑1 (MQ=255)
                                                                                                         ccGCCTTGTACTTTCTTAAACCGAAATTGGTTAGAGCAGTAATGTTGATACTGCCGGTTTTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTgaa                          <  3:297912/138‑1 (MQ=255)
                                                                                                         ccGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTgaa                          <  5:35846/138‑1 (MQ=255)
                                                                                                                  aCTTTCTGAAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGGaaa                 <  5:385839/138‑1 (MQ=255)
                                                                                                                      tCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGGAAAGCCGt            >  7:182881/1‑139 (MQ=255)
                                                                                                                                aCATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGGAAAGCCGTTCAAGCttcg  >  8:139183/1‑137 (MQ=255)
                                                                                                                                        |                                                                                                                                  
AAAATAAAAATCCGCTTCCGCGCTGACATTTGATAGAAAAAAGGCGGCAG‑CAGCCGCCCCT‑‑‑‑CTTATTTAGGGTAGTCCCGTTTTTTCAGCTTGTTTTTCTCCGCTTTGTACTTTCTGTAGCCGACATAGGTCAGAGCTGTAATGATGATACTGCCGGTTGTGATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAAAACATTTTTCAGATCATGCTGAAGAAGAGAAAGCCGTTCAAGCTTTG  >  CP006881/2179329‑2179590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑