| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,179,490 | G→A | I232I (ATC→ATT) | U712_10910 ← | Uncharacterized protein ypjB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,179,490 | 0 | G | A | 98.3% | 181.8 / ‑4.5 | 60 | I232I (ATC→ATT) | U712_10910 | Uncharacterized protein ypjB |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (35/24); total (35/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01 | |||||||||||
ATAGAAAAAAGGCGGCAG‑CAGCCGCCCCT‑‑‑‑CTTATTTAGGGTAGTCCCGTTTTTTCAGCTTGTTTTTCTCCGCTTTGTACTTTCTGTAGCCGACATAGGTCAGAGCTGTAATGATGATACTGCCGGTTGTGATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAAAACATTTTTCAGATCATGCTGAAGAAGAGAAAGCCGTTCAAGCTTTGTGCTCTCAGTCATTTGCTGAAATTCCTCTTG > CP006881/2179361‑2179621 | aTAGAAAAAAGGCGGCTGACCGCCGCCCCTCATCCTTATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGtaatga < 1:110579/139‑1 (MQ=255) ccT‑‑‑‑CTTATTTAGGGTAGTCCCGTTTTTTCAGCTTGTTTTTCTCCGCTTTGAACTTTCTGTAGCCGACATAGGGCAGAGCTGTAATGATGATACTGCCGGTTGTGATGATCACCCAGAGTAAAGACGGATCGGCatcatc < 3:338585/139‑1 (MQ=255) tATTTAGGGTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCAtcctcct > 8:250836/1‑139 (MQ=255) ggTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCt > 4:91417/1‑139 (MQ=255) ggTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCt > 7:33672/1‑139 (MQ=255) ggTAGTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCt > 6:333539/1‑139 (MQ=255) gTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCtctc > 3:105017/1‑138 (MQ=255) gTCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTc < 8:24574/138‑1 (MQ=255) tCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCa > 5:321274/1‑138 (MQ=255) tCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCa > 3:349851/1‑138 (MQ=255) tCCCTTTTTTTCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCa > 6:73420/1‑138 (MQ=255) cttttattCATCTTGTTTTTATCCGCCTTGTTCTTTCTGAACCCGAAATAGGTAAGACCAGTAATGATGATACTGCCGGTTGTATTGATCACCAAGATTAAAGACGGATCGGCATCATCCTCCTCCACCGAGTCaaaca < 2:286956/137‑3 (MQ=255) tttttCAGCTTGTTTTTTTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAAcaca < 5:361408/138‑1 (MQ=255) ttttttTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCAt > 3:381844/1‑139 (MQ=255) ttttttTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAAAACATTTTTCAGGTCAt > 5:251189/1‑139 (MQ=255) ttttgccGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTATTGATCACCCAGAGTACCGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATg < 5:348531/133‑1 (MQ=255) ttttTCCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCATTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATg < 8:34112/138‑1 (MQ=255) cttttcCGCCTTGTCCTTTCTCTAACCCACATACGCACGAGCAGTAATGATGATACTGCCGGTGGTAATGAACACCCAGACAAACCACGGATCGGCATCATCCTCCTCCACCCTGCCAAACACATTTTTCAGGTCATg < 4:349851/134‑1 (MQ=255) ttcCGCGTTGTACTTTCGGTAACCGACTTAGGTAAGAGCAGTATTGATGATACTGCCGGTTGTAATTCTCACCCAGAGTAATGACGGATCGGCATGATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTg < 7:221497/137‑1 (MQ=255) ttcCGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGTTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTg < 3:153208/137‑1 (MQ=255) cggccttGTACTTGCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTgaa < 3:131981/133‑1 (MQ=255) ccGCCTTGTACTTTCTTAAACCGAAATTGGTTAGAGCAGTAATGTTGATACTGCCGGTTTTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTgaa < 3:297912/138‑1 (MQ=255) ccGCCTTGTACTTTCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTgaa < 5:35846/138‑1 (MQ=255) aCTTTCTGAAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGGaaa < 5:385839/138‑1 (MQ=255) tCTGTAACCGACATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGGAAAGCCGt > 7:182881/1‑139 (MQ=255) aCATAGGTAAGAGCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGGAAAGCCGTTCAAGCttcg > 8:139183/1‑137 (MQ=255) gtaagagCAGTAATGATTATACTGCCGTTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGGCATGCTGAAGAAGGGAAAGCCGTTCAAGCTTCGTGctct < 6:208171/136‑1 (MQ=255) gtaagagCAGTAAAGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGAAGGATCGGCATCATCCTCCTCCACCCTGTCAAACAAATTTTTCAGGTCATGCTGAATAAGGGAAAGCCGTACACGCATCGAGctc > 1:381281/4‑138 (MQ=255) agCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGAt < 3:118470/53‑1 (MQ=255) agCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGAt > 4:118470/1‑53 (MQ=255) agCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCGTCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTGATGAAGGGAAAGCCGGTCAAGCTTCGTGCTCTCTGTc > 6:187002/1‑139 (MQ=255) agCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATg < 3:136329/99‑1 (MQ=255) agCAGTAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATg > 4:136329/1‑99 (MQ=255) tAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCatcatc > 8:409680/1‑58 (MQ=255) tAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCatcatc < 7:409680/58‑1 (MQ=255) tAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGGAAAGCCGTTCAAGCTTCGTGCTCTCTGTCATTTg > 5:134990/1‑139 (MQ=255) tAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGGAAAGCCGTTCAAGCTTCGTGCTCTCTGTCATTTg > 8:186169/1‑139 (MQ=255) tAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGGAAAGCCGTTCAAGCTTCGTGCTCTCTGTCATTTg > 5:310221/1‑139 (MQ=255) tAATGATGATACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGGAAAGCCGTTCAAGCTTCGTGCTCTCTGTCATTTg > 6:187498/1‑139 (MQ=255) tAATGATGATACTGCCGCTTGTAATCATCACACAGAGTAAAGACGGAACGGCATCATCCTCATCCACCCTGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGGAAAGCCGTTCAAGCTACGTGCTCTCTGTCAttt > 3:158570/1‑138 (MQ=255) gatgatACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGGAAAGCCGTTCAAGCTTCGTGCTCTCTGTCATTTGCTg > 5:232807/1‑138 (MQ=255) gatgatACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCACCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGTAAAGCCGTTCAAGCTTCGTGCTCTCTGTCATTTGCTg > 1:410595/1‑138 (MQ=255) gatgatACTGCCGGTTGTAATGATCACCAAGAGTAAAGACGGATCGGCATCATCCTCCTGCACCCTGTCAAACACATTTTTCATGTCATGCTGAAGAAGGGAAAGCCGTTCAAACTTCGTGCTCTCTGTCAtaggatc > 7:168211/1‑132 (MQ=255) atgatACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGGAAAGCCGTTCAAGCTTCGTGCTCTCTGTCATTTGCTGa > 3:83045/1‑138 (MQ=255) atgatACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGGAAAGCCGTTCAAGCTTCGTGCTCTCTGTCATTTGCTGa > 8:178507/1‑138 (MQ=255) atgatACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCATCCTCCACCATGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGGAAAGCCGATCAAGCTTCGTGCTCTCTGTCATTTGCTGa > 7:75020/1‑138 (MQ=255) gatACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGGAAAGCCGTTCAAGCTTCGTGct > 6:397683/1‑120 (MQ=255) gatACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGGAAAGCCGTTCAAGCTTCGTGct < 5:397683/120‑1 (MQ=255) atACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGGAAAGCCGTTCAAGCTTCGTGCTCTCTGTCATTTGCTGAAAtt > 4:442280/1‑139 (MQ=255) atACTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGGAAAGCCGTTCAAGCTTCGTGCTCTCTGTCATTTGCTGAAAtt > 5:174281/1‑139 (MQ=255) aCTGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGGTAAGCCGTTCAAGCTTCGTGCTCTCTGTCATTTGCTGAAATTcc > 7:316164/1‑139 (MQ=255) tGCCGGTTGTAATGATCACCCAGAGTAAAGACGGAt > 2:388666/1‑36 (MQ=255) tGCCGGTTGTAATGATCACCCAGAGTAAAGACGGAt < 1:388666/36‑1 (MQ=255) tGCCGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGGAAAGCCGTTCAAGCTTCGTGCTCTCTGTCATTTGCTGAAATTCctc > 4:267068/1‑139 (MQ=255) ccGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCCTGCTGAAGAAGGGAAAGCCGTTCAAGCTTCGTGCTCTCTGTCATTTGCTGAAATTCctct < 4:105017/138‑1 (MQ=255) ccGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGGAAAGCCGTTCAAGCTTCGTGCTCTCTGTCATTTGGTGAACTTCCTCtt > 8:224549/1‑139 (MQ=255) ccGGGTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTACAACCTGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGGAAAGCCGTTCAAGCTTCGTGCTCTCTGTCATTTGCTGAAATTCctc < 5:303360/137‑1 (MQ=255) cGGTTTTAATGATCACCCAGTTTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAATGACATTTTTCAGGTCATGCTGAAGAAGGGAATGCCGTTCAAGCTTCGTGCTCTCTGTCATTTGCTGAAATTCCTCTTg < 4:158570/139‑1 (MQ=255) cGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGGAAAGCCGTTCAAGCTTCGTGCTCTCTGTCATTTGCTGAAATTCCTCTTg < 2:281487/139‑1 (MQ=255) cGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGGAAAGCCGTTCAAGCTTCGTGCTCTCTGTCATTTGCTGAAATTCCTCTTg < 2:156919/139‑1 (MQ=255) cGGTTGTAATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAACACATTTTTCAGGTCATGCTGAAGAAGGGAAAGCCGTTCAAGCTTCGTGCTCTCTGTCATTTGCTGAAATTCCTCTTg < 8:369165/139‑1 (MQ=255) | ATAGAAAAAAGGCGGCAG‑CAGCCGCCCCT‑‑‑‑CTTATTTAGGGTAGTCCCGTTTTTTCAGCTTGTTTTTCTCCGCTTTGTACTTTCTGTAGCCGACATAGGTCAGAGCTGTAATGATGATACTGCCGGTTGTGATGATCACCCAGAGTAAAGACGGATCGGCATCATCCTCCTCCACCCTGTCAAAAACATTTTTCAGATCATGCTGAAGAAGAGAAAGCCGTTCAAGCTTTGTGCTCTCAGTCATTTGCTGAAATTCCTCTTG > CP006881/2179361‑2179621 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |