Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,179,688	A→G	P166P (CCT→CCC)	U712_10910 ←	Uncharacterized protein ypjB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,179,688	0	A	G	100.0%	207.2 / NA	64	P166P (CCT→CCC)	U712_10910	Uncharacterized protein ypjB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/28);  total (36/28)

ATGCTGAAGAAGAGAAAGCCGTTCAAGCTTTGTGCTCTCAGTCATTTGCTGAAATTCCTCTTGCTCAATGACGTCTATATGTTTTCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTAGGGTAAATCAAATCATATAAGCTGATAAATTCATTCCATTTTTCATGAAAGCTCGTACTTTCATTCTTTTGGACATCCTGCTTCAGTTCAGCAAAGGCTTCCATAATCGGTCTTTCCAGTGAGCCCCAAAG  >  CP006881/2179559‑2179819                                                                                                                                  |                                                                                                                                    aTGCTGAAGAAGGGAAAGCCGTTCAAGCTTCGTGCTCTCTGTCATTTGCTGAAATTCCTCTTGCTCAATAACATCTATATGTTTTCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTaaat                                                                                                                             >  6:337699/1‑138 (MQ=255)    cTGAAGAAGGGAAAGCCGTTCAAGCTTCGTGCTCTCTGTCATTTGCTGAAATTCCTCTTGCTCAATAACATCTATATGTTTTCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTaaatcaa                                                                                                                          <  3:335294/138‑1 (MQ=255)    cTGAAGAAGGGAAAGCCGTTCAAGCTTCGTGCTCTCTGTCATTTGCTGAAATTCCTCTTGCTCAATAACATCTATATGTTTTCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTaaatcaa                                                                                                                          <  2:359642/138‑1 (MQ=255)         gaaggGAAAGCCGTTCAAGCTTCGTGCTCTCTGTCATTTGCTGAAATTCCTCTTGCTCAATAACATCTATATGTTTTCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCata                                                                                                                    >  7:28200/1‑139 (MQ=255)             ggaAAGCCGTTCAAGCTTCGTGCTCTCTGTCATTTGCTGAAATTCCTCTTGCTCAATAACATCTATATGTTTTCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATaaa                                                                                                                <  8:33672/138‑2 (MQ=255)                      ttaaaGCTTCGTGCTCTCTGTCATTTGCTGAAATTCCTCTTGCTCAATAACATCTATATGTTTTCCGACTGTTTCAAGCTGATCCTCTGATACAACGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAat                                                                                                       >  8:414258/4‑139 (MQ=255)                        cAAGCTTCGTGCTCTCTGTCATTTGCTGAAATTCCTCTTGCTCAATAACATCTATATGTTTTCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAatt                                                                                                      <  7:219971/138‑1 (MQ=255)                                 tgCTCTCTGTCATTTGCTGAAATTCCTCTTGCTCAATAACATCTATATGTTTTCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCAttt                                                                                            <  1:101506/139‑1 (MQ=255)                                          tCATTTGCTGAAATTCCTCTTGCTCAATAACATCTATATGTTTTCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATg                                                                                      <  5:299072/136‑1 (MQ=255)                                          tCATTTGCTGAAATTCCTCTTGCTCAATAACATCTATATGTTTTCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATg                                                                                      <  5:337699/136‑1 (MQ=255)                                          tCATTTGCTGAAATTCCTCTTGCTCAATAACATCTATATGTTTTCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGaa                                                                                    <  6:232807/138‑1 (MQ=255)                                          gcATTTGCTGAAATTCCTCTTGCTCAATAACATCTATATGTTTTCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATg                                                                                      <  8:316164/135‑1 (MQ=255)                                             tttGCTGAAATTCCTCTTGCTCAATAACATCTATATGTTTTCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCt                                                                                <  7:414258/139‑1 (MQ=255)                                                gCTGAAATTCCTCTTGCTCAATAACATCTATATGTTTTCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCttg                                                                              <  2:337953/138‑3 (MQ=255)                                                gCTGAAATTCCTCTTGCTCAATAACATCTATATGTTTTCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGt                                                                             >  7:186680/1‑139 (MQ=255)                                                  tGAAATTCCTCTTGCTCAATAACATCTATATGTTTTCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGtgc                                                                           >  8:160178/1‑137 (MQ=255)                                                  tGAAATTCCTCTTGCTCAATAACATCTATATGTTTTCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGtgc                                                                           >  1:251282/1‑137 (MQ=255)                                                       ttCCTCTTGCTCAATAACATCTATATGTTTTCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCa                                                                      <  4:446597/139‑1 (MQ=255)                                                                     taacatCTATATGTTTTCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATc                                                        <  6:174281/134‑1 (MQ=255)                                                                       acatCTATATGTTTTCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATct                                                       <  8:28200/135‑2 (MQ=255)                                                                       acatCTATATGTTATCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATct                                                       <  8:200880/135‑2 (MQ=255)                                                                          tCTATATGTTTTCCGCCTGTTTCAAGCAGATCCTCTCATACATCGAATGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCAATTCTTTTGGACATctcgct                                                   >  7:140833/1‑134 (MQ=255)                                                                             atatGTTTTCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCttca                                                >  6:247474/1‑139 (MQ=255)                                                                                   tttCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGa                                                                                     <  8:279970/96‑1 (MQ=255)                                                                                   tttCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGa                                                                                     >  7:279970/1‑96 (MQ=255)                                                                                   tttCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCttcagttcag                                          >  8:137397/1‑139 (MQ=255)                                                                                    ttCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTaaatcaa                                                                                                                          >  6:170701/1‑58 (MQ=255)                                                                                    ttCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTaaatcaa                                                                                                                          <  5:170701/58‑1 (MQ=255)                                                                                    ttCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTATGGACATCTCGCttcagttcag                                          >  5:309327/1‑138 (MQ=255)                                                                                     tCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCttcagttcagt                                         >  4:113687/1‑137 (MQ=255)                                                                                     tCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCttcagttcagt                                         >  5:433450/1‑137 (MQ=255)                                                                                     tCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCttcagttcagt                                         >  6:127784/1‑137 (MQ=255)                                                                                     tCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCttcagttcagt                                         >  8:315822/1‑137 (MQ=255)                                                                                     tCCGACTGTTTCAAGCT‑ATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGTACATCTCGCttcagttcagta                                        >  4:251744/1‑136 (MQ=255)                                                                                         aCTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCtt                                                  >  2:385099/1‑125 (MQ=255)                                                                                         aCTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCtt                                                  <  1:385099/125‑1 (MQ=255)                                                                                                       aTCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATAATCGGt                      >  4:344162/1‑139 (MQ=255)                                                                                                       aTCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATAATCGGt                      >  1:84851/1‑139 (MQ=255)                                                                                                       aTCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATAATCGGt                      >  6:255110/1‑139 (MQ=255)                                                                                                       aTCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATAATCGGt                      >  5:40201/1‑139 (MQ=255)                                                                                                       aTCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATAATCGGt                      >  1:34445/1‑139 (MQ=255)                                                                                                       aTCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATATCGCTTCAGTTCAGTAAAAGCTTCCATAATCGGt                      >  3:386896/1‑139 (MQ=255)                                                                                                       aTCCTCTGATACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCACTCTTTTGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATAATCGGt                      >  5:11441/1‑139 (MQ=255)                                                                                                               aTACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATAATCGGTTTTTCCAg              >  7:239734/1‑139 (MQ=255)                                                                                                                tACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATAATCGGTTTTTCCAGt             <  6:40201/139‑1 (MQ=255)                                                                                                                tACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATAATCGGTTTTTCCAGt             <  5:247474/139‑1 (MQ=255)                                                                                                                tACATCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTATGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATAATCGGTTTTTCCAGt             <  6:309327/139‑1 (MQ=255)                                                                                                                    tCGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATATATCAAGAAAGCTAGTGCTACCATTCTATTGGACATCTCGCTTCAGTACAGAAAAAGCTTCCATAATCGTTTTTTCCAGTGAGc         >  3:270005/1‑139 (MQ=255)                                                                                                                     cGATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATAATCGGTTTTTCCAGTGAGcc        >  6:270455/1‑139 (MQ=255)                                                                                                                      gATTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCACTCTTTTGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATAATCGGTTTTTCCAGTGAGccc       <  6:11441/139‑1 (MQ=255)                                                                                                                       aTTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATaa                           <  7:240403/118‑1 (MQ=255)                                                                                                                       aTTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATaa                           >  8:240403/1‑118 (MQ=255)                                                                                                                       aTTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATAATCGGTTTTTCCAGt             <  5:300640/132‑1 (MQ=255)                                                                                                                       aTTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATAATCGGTTTTTCCAGt             >  6:300640/1‑132 (MQ=255)                                                                                                                       aTTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATAATCGGTTTTTCCAGTGAGccc       >  3:96381/1‑138 (MQ=255)                                                                                                                       aTTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATAATCGGTTTTTCCAGTGAGccc       >  2:371256/1‑138 (MQ=255)                                                                                                                       aTTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATAATCGGTTTTTCCAGTGAGccc       >  2:142404/1‑138 (MQ=255)                                                                                                                       aTTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCTTCAGTTCAGAAAAAGCTTCCATAATCGGTTTTTCCAGTg            >  8:418693/1‑133 (MQ=255)                                                                                                                       aTTGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCTTCAGTTCAGAAAAAGCTTCCATAATCGGTTTTTCCAGTg            <  7:418693/133‑1 (MQ=255)                                                                                                                         tGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATAATCGGTTTTTCCAGTGAGCCCCa     >  7:86971/1‑138 (MQ=255)                                                                                                                         tGTAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATAATCGGTTTTTCCAGTGAGCCCCa     >  3:128944/1‑138 (MQ=255)                                                                                                                           tAAGACTGGGGTAAATCAAGTCATATAATCTGATAAATTGATTGCATTTTTGATGAAAGCTTGTGCTTCGATTCTTTTGGACATCTCGCTTCAGTTGAGTAAAAGCTTCCATAATCGGTTTTTCCAGTGAGCCCCAAAg  <  4:270005/139‑1 (MQ=255)                                                                                                                           tAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATAATCGGTTTTTCCAGTGAGCCCCaaa   <  1:142404/138‑1 (MQ=255)                                                                                                                           tAAGACTGGGGTAAATCAAGTCATATAAACTGATAAATTCATTCCATTTTTCATGAAAGCTTGTGCTTCCATTCTTTTGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATAATCGGTTTTTCCAGTGAGCCCCAAAg  <  6:433450/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                    ATGCTGAAGAAGAGAAAGCCGTTCAAGCTTTGTGCTCTCAGTCATTTGCTGAAATTCCTCTTGCTCAATGACGTCTATATGTTTTCCGACTGTTTCAAGCTGATCCTCTGATACATCGATTGTAAGACTAGGGTAAATCAAATCATATAAGCTGATAAATTCATTCCATTTTTCATGAAAGCTCGTACTTTCATTCTTTTGGACATCCTGCTTCAGTTCAGCAAAGGCTTCCATAATCGGTCTTTCCAGTGAGCCCCAAAG  >  CP006881/2179559‑2179819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑