Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,179,880 C→T K102K (AAG→AAA)  U712_10910 ← Uncharacterized protein ypjB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,179,8800CT98.6% 215.3 / NA 70K102K (AAG→AAA) U712_10910Uncharacterized protein ypjB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (27/42);  minor base G (0/1);  total (27/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCATTCTTTTGGACATCCTGCTTCAGTTCAGCAAAGGCTTCCATAATCGGTCTTTCCAGTGAGCCCCAAAGCGGATCAGATCGATTGTCAACTGCGTCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGCTTTTGTGTATCTGATGTATCAGCCTGCTTTAAACTCCGTACCATATCATTATAGCCCAGTGTGATTTGACGGATTTGGGCTCCCGTTAGCATGGTGTCTTGCTTTTTTTTCTCGGCGGATTTTAATGTTT  >  CP006881/2179749‑2180010
                                                                                                                                   |                                                                                                                                  
ccATTCTTTTGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTtgt                                                                                                                              <  4:120593/137‑1 (MQ=255)
  aTTCTTTTGGACAACTCGAGTCAGATCAGTAAAAGCTTCCATAATCGGGTTTTCCATGGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCAGCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTgtgtg                                                                                                                           <  4:228714/139‑2 (MQ=255)
     cTTTTGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTc                                                                                                                         <  8:86971/138‑1 (MQ=255)
        ttGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCt                                                                                                                        <  2:251282/136‑1 (MQ=255)
        ttGGACATCTCGCTTCAGTTCAGTAAAAGCTTCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTg                                                                                                                       <  3:307776/137‑1 (MQ=255)
                ctcgCTTCAGTTCAGTAAAAGCTTCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGc                                                                                                             >  2:163923/4‑139 (MQ=255)
                   gCTTCAGTTCAGTAAAAGCTTCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGc                                                                                                             <  8:239734/136‑1 (MQ=255)
                   gCTTCAGTTCAGTAAAAGCTTCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGc                                                                                                             <  4:96381/136‑1 (MQ=255)
                   gCTTCAGTTCAGTAAAAGCTTCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGc                                                                                                             <  3:344162/136‑1 (MQ=255)
                   gCTTCAGTTCAGTAAAAGCTTCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGc                                                                                                             <  3:299747/136‑1 (MQ=255)
                   gCTTCAGTTCAGTAAAAGCTTCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGc                                                                                                             <  3:251744/136‑1 (MQ=255)
                        agttcagTAAAAGCTTCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTa                                                                                                     <  1:100249/139‑1 (MQ=255)
                               taaaaGCTTCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTcc                                                                                               >  3:259374/2‑138 (MQ=255)
                               taaaaGCTTCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCg                                                                                              <  4:397853/138‑1 (MQ=255)
                                  aagCTTCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAAc                                                                                           <  7:315822/137‑1 (MQ=255)
                                    gCTTCCATAATCGGTTTTTCCAGTGAGCCCCATAGCGGGTCAGACCGATTGTCAAGCGCATCCATCAGCATTCTGAACTGCGCGTCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCa                                                                                         <  4:128944/139‑1 (MQ=255)
                                    gCTTCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCa                                                                                         >  2:308261/1‑139 (MQ=255)
                                       tCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                      >  2:430312/1‑139 (MQ=255)
                                       tCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCat                                                                                        >  3:111352/1‑137 (MQ=255)
                                       tCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCat                                                                                        <  4:111352/137‑1 (MQ=255)
                                       tCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTGTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                      <  5:187002/139‑1 (MQ=255)
                                        ccATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                      <  5:127784/138‑1 (MQ=255)
                                        ccATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                      <  5:270455/138‑1 (MQ=255)
                                        ccATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                      <  1:276380/138‑1 (MQ=255)
                                        ccATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                      <  6:45655/138‑1 (MQ=255)
                                        ccATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                      <  7:137397/138‑1 (MQ=255)
                                        ccATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTGTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                      <  8:393750/138‑1 (MQ=255)
                                        ccATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGGTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                      <  3:416510/138‑1 (MQ=255)
                                        ccATAATCGGTTTTTCCAGTGAGCACCAAAGCGTGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                      <  3:227994/138‑1 (MQ=255)
                                         cATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGctt                                                                                                           <  8:101962/116‑3 (MQ=255)
                                         cATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGctt                                                                                                           >  7:101962/1‑114 (MQ=255)
                                         cATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATAAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCa                                                                                    >  6:184007/1‑139 (MQ=255)
                                             aTCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATta                                                                                 <  3:113687/138‑1 (MQ=255)
                                             aTCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATta                                                                                 <  1:163923/138‑1 (MQ=255)
                                                  tttttCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGcc                                                                            >  3:168307/3‑138 (MQ=255)
                                                  tttttCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGcc                                                                            >  8:116599/3‑138 (MQ=255)
                                                            gAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGAttt                                                                 >  5:428582/1‑139 (MQ=255)
                                                                          gtCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCt                                                   >  2:238863/2‑139 (MQ=255)
                                                                          gtCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCt                                                   <  2:55382/138‑1 (MQ=255)
                                                                          gtCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGAGATTTGACGAATTTGGGCt                                                   >  3:730/2‑139 (MQ=255)
                                                                              gaccGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCg                                               <  1:430312/136‑1 (MQ=255)
                                                                                    ttGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTtg                                                                                                                               <  2:98656/53‑1 (MQ=255)
                                                                                    ttGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTtg                                                                                                                               >  1:98656/1‑53 (MQ=255)
                                                                                    ttGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCa                                         <  1:371256/139‑1 (MQ=255)
                                                                                         aaCCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                      <  8:384221/89‑1 (MQ=255)
                                                                                         aaCCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                      >  7:384221/1‑89 (MQ=255)
                                                                                         aaCCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATgggg                                    >  1:236179/1‑137 (MQ=255)
                                                                                              catCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCGCGt                                              <  3:196149/122‑1 (MQ=255)
                                                                                              catCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGt                                              >  4:196149/3‑124 (MQ=255)
                                                                                               atCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGc                              <  4:168307/138‑1 (MQ=255)
                                                                                               atCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGc                              <  7:116599/138‑1 (MQ=255)
                                                                                                          ttCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTtct                    >  2:9673/1‑138 (MQ=255)
                                                                                                           tCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                      <  2:309005/71‑1 (MQ=255)
                                                                                                           tCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                      >  1:309005/1‑71 (MQ=255)
                                                                                                               aaCTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTtctcag                 <  2:236179/136‑3 (MQ=255)
                                                                                                               aaCTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTcagc                <  4:259374/137‑1 (MQ=255)
                                                                                                                 cTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                      <  7:233946/65‑1 (MQ=255)
                                                                                                                 cTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                      >  8:233946/1‑65 (MQ=255)
                                                                                                                  tGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGa                                                               >  7:227461/1‑87 (MQ=255)
                                                                                                                  tGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGa                                                               <  8:227461/87‑1 (MQ=255)
                                                                                                                  tGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCGGTTTTTTGTCAGCGGCtt           >  6:297602/1‑139 (MQ=255)
                                                                                                                  tGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTGGCTGTTTTTTCTCAGCGGAtt           >  1:403778/1‑139 (MQ=255)
                                                                                                                    cgcgGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGa                                                               >  7:150899/1‑85 (MQ=255)
                                                                                                                    cgcgGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGa                                                               >  3:407283/1‑85 (MQ=255)
                                                                                                                    cgcgGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGa                                                               <  8:150899/85‑1 (MQ=255)
                                                                                                                    cgcgGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGa                                                               <  4:407283/85‑1 (MQ=255)
                                                                                                                    cgcgGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTCAGCGGAtttt         >  6:268382/1‑139 (MQ=255)
                                                                                                                    cgcgGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCACAGGGGAtttt         >  2:208294/1‑139 (MQ=255)
                                                                                                                    cgcgGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATCGGGTCTTGCTGTGTTTTCTCAGAGGAttt          >  5:53142/1‑138 (MQ=255)
                                                                                                                            ctctGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTCAGCGGATTTTAATGttt  <  2:34445/138‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                  
TCATTCTTTTGGACATCCTGCTTCAGTTCAGCAAAGGCTTCCATAATCGGTCTTTCCAGTGAGCCCCAAAGCGGATCAGATCGATTGTCAACTGCGTCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGCTTTTGTGTATCTGATGTATCAGCCTGCTTTAAACTCCGTACCATATCATTATAGCCCAGTGTGATTTGACGGATTTGGGCTCCCGTTAGCATGGTGTCTTGCTTTTTTTTCTCGGCGGATTTTAATGTTT  >  CP006881/2179749‑2180010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑