Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,179,904	C→T	Q94Q (CAG→CAA)	U712_10910 ←	Uncharacterized protein ypjB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,179,904	0	C	T	100.0%	196.6 / NA	63	Q94Q (CAG→CAA)	U712_10910	Uncharacterized protein ypjB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/36);  total (27/36)

AGTTCAGCAAAGGCTTCCATAATCGGTCTTTCCAGTGAGCCCCAAAGCGGATCAGATCGATTGTCAACTGCGTCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGCTTTTGTGTATCTGATGTATCAGCCTGCTTTAAACTCCGTACCATATCATTATAGCCCAGTGTGATTTGACGGATTTGGGCTCCCGTTAGCATGGTGTCTTGCTTTTTTTTCTCGGCGGATTTTAATGTTTTTTCAAAATAAGCAAGGACTTGCAGCGCC  >  CP006881/2179773‑2180039                                                                                                                                    |                                                                                                                                        agttcagTAAAAGCTTCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTa                                                                                                                                  <  1:100249/139‑1 (MQ=255)        taaaaGCTTCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTcc                                                                                                                            >  3:259374/2‑138 (MQ=255)        taaaaGCTTCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCg                                                                                                                           <  4:397853/138‑1 (MQ=255)           aagCTTCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAAc                                                                                                                        <  7:315822/137‑1 (MQ=255)             gCTTCCATAATCGGTTTTTCCAGTGAGCCCCATAGCGGGTCAGACCGATTGTCAAGCGCATCCATCAGCATTCTGAACTGCGCGTCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCa                                                                                                                      <  4:128944/139‑1 (MQ=255)             gCTTCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCa                                                                                                                      >  2:308261/1‑139 (MQ=255)                tCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                                                   >  2:430312/1‑139 (MQ=255)                tCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCat                                                                                                                     >  3:111352/1‑137 (MQ=255)                tCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCat                                                                                                                     <  4:111352/137‑1 (MQ=255)                tCCATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTGTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                                                   <  5:187002/139‑1 (MQ=255)                 ccATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                                                   <  5:127784/138‑1 (MQ=255)                 ccATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                                                   <  5:270455/138‑1 (MQ=255)                 ccATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                                                   <  6:45655/138‑1 (MQ=255)                 ccATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                                                   <  7:137397/138‑1 (MQ=255)                 ccATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                                                   <  1:276380/138‑1 (MQ=255)                 ccATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTGTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                                                   <  8:393750/138‑1 (MQ=255)                 ccATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGGTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                                                   <  3:416510/138‑1 (MQ=255)                 ccATAATCGGTTTTTCCAGTGAGCACCAAAGCGTGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                                                   <  3:227994/138‑1 (MQ=255)                  cATAATCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATAAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCa                                                                                                                 >  6:184007/1‑139 (MQ=255)                      aTCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATta                                                                                                              <  1:163923/138‑1 (MQ=255)                      aTCGGTTTTTCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATta                                                                                                              <  3:113687/138‑1 (MQ=255)                           tttttCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGcc                                                                                                         >  3:168307/3‑138 (MQ=255)                           tttttCCAGTGAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGcc                                                                                                         >  8:116599/3‑138 (MQ=255)                                     gAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGAttt                                                                                              >  5:428582/1‑139 (MQ=255)                                                   gtCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCt                                                                                <  2:55382/138‑1 (MQ=255)                                                   gtCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCt                                                                                >  2:238863/2‑139 (MQ=255)                                                   gtCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGAGATTTGACGAATTTGGGCt                                                                                >  3:730/2‑139 (MQ=255)                                                       gaccGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCg                                                                            <  1:430312/136‑1 (MQ=255)                                                             ttGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCa                                                                      <  1:371256/139‑1 (MQ=255)                                                                  aaCCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                                                   <  8:384221/89‑1 (MQ=255)                                                                  aaCCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                                                   >  7:384221/1‑89 (MQ=255)                                                                  aaCCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATgggg                                                                 >  1:236179/1‑137 (MQ=255)                                                                       catCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCGCGt                                                                           <  3:196149/122‑1 (MQ=255)                                                                       catCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGt                                                                           >  4:196149/3‑124 (MQ=255)                                                                        atCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGc                                                           <  4:168307/138‑1 (MQ=255)                                                                        atCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGc                                                           <  7:116599/138‑1 (MQ=255)                                                                                   ttCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTtct                                                 >  2:9673/1‑138 (MQ=255)                                                                                    tCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                                                   >  1:309005/1‑71 (MQ=255)                                                                                    tCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                                                   <  2:309005/71‑1 (MQ=255)                                                                                        aaCTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTtctcag                                              <  2:236179/136‑3 (MQ=255)                                                                                        aaCTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTcagc                                             <  4:259374/137‑1 (MQ=255)                                                                                          cTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                                                   >  8:233946/1‑65 (MQ=255)                                                                                          cTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCatat                                                                                                                   <  7:233946/65‑1 (MQ=255)                                                                                           tGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGa                                                                                            <  8:227461/87‑1 (MQ=255)                                                                                           tGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGa                                                                                            >  7:227461/1‑87 (MQ=255)                                                                                           tGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCGGTTTTTTGTCAGCGGCtt                                        >  6:297602/1‑139 (MQ=255)                                                                                           tGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTGGCTGTTTTTTCTCAGCGGAtt                                        >  1:403778/1‑139 (MQ=255)                                                                                             cgcgGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGa                                                                                            <  4:407283/85‑1 (MQ=255)                                                                                             cgcgGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGa                                                                                            <  8:150899/85‑1 (MQ=255)                                                                                             cgcgGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGa                                                                                            >  3:407283/1‑85 (MQ=255)                                                                                             cgcgGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGa                                                                                            >  7:150899/1‑85 (MQ=255)                                                                                             cgcgGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTCAGCGGAtttt                                      >  6:268382/1‑139 (MQ=255)                                                                                             cgcgGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCACAGGGGAtttt                                      >  2:208294/1‑139 (MQ=255)                                                                                             cgcgGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATCGGGTCTTGCTGTGTTTTCTCAGAGGAttt                                       >  5:53142/1‑138 (MQ=255)                                                                                                     ctctGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTCAGCGGATTTTAATGttt                               <  2:34445/138‑1 (MQ=255)                                                                                                          agtttttGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGCATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTGTTTTCTGAGCGGATTTTAATGTTTTTTcc                          >  5:405256/4‑137 (MQ=255)                                                                                                               ttGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTcagcg                                            <  6:124920/115‑1 (MQ=255)                                                                                                               ttGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTcagcg                                            >  5:124920/1‑115 (MQ=255)                                                                                                                  tgtgTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTCAGCGGATTTTAATGTTTTTTCAAAATactcc                 <  1:308261/139‑5 (MQ=255)                                                                                                                  tgtgTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTCAGCGGATTTTAATGTTTTTTCAAAATactcc                 <  6:428582/139‑5 (MQ=255)                                                                                                                              atcatcTTGCTTTAAACTCCGAACCATATCATTATCGCCAAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTGGCTGTTTTTTCTCAGCGGATTTTAATGTTTTTTCAAAATACTCCAAGACTTGAAg      <  2:259802/138‑1 (MQ=255)                                                                                                                                cAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTCAGCGGATTTTAATGTTTTTTCAAAATACTCCAAGACTTGAAgcgc   >  6:419772/1‑139 (MQ=255)                                                                                                                                 aGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTCAGCGGATTTTAATGTTTTTTCAAAATACTCCAAGACTTGAAGCGcc  <  7:52651/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        AGTTCAGCAAAGGCTTCCATAATCGGTCTTTCCAGTGAGCCCCAAAGCGGATCAGATCGATTGTCAACTGCGTCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGCTTTTGTGTATCTGATGTATCAGCCTGCTTTAAACTCCGTACCATATCATTATAGCCCAGTGTGATTTGACGGATTTGGGCTCCCGTTAGCATGGTGTCTTGCTTTTTTTTCTCGGCGGATTTTAATGTTTTTTCAAAATAAGCAAGGACTTGCAGCGCC  >  CP006881/2179773‑2180039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑