Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,179,939 G→A L83L (CTG→TTG)  U712_10910 ← Uncharacterized protein ypjB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,179,9390GA98.3% 174.0 / NA 58L83L (CTG→TTG) U712_10910Uncharacterized protein ypjB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (29/28);  minor base T (0/1);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGCCCCAAAGCGGATCAGATCGATTGTCAACTGCGTCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGCTTTTGTGTATCTGATGTATCAGCCTGCTTTAAACTCCGTACCATATCATTATAGCCCAGTGTGATTTGACGGATTTGGGCTCCCGTTAGCATGGTGTCTTGCTTTTTTTTCTCGGCGGATTTTAATGTTTTTTCAAAATAAGCAAGGACTTGCAGCGCCTCTTCATATTTTGACTGGCGGGTCATTTGAAAGAC  >  CP006881/2179809‑2180074
                                                                                                                                  |                                                                                                                                       
gAGCCCCAAAGCGGGTCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGAttt                                                                                                                                 >  5:428582/1‑139 (MQ=255)
              gtCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCt                                                                                                                   <  2:55382/138‑1 (MQ=255)
              gtCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCt                                                                                                                   >  2:238863/2‑139 (MQ=255)
              gtCAGACCGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGAGATTTGACGAATTTGGGCt                                                                                                                   >  3:730/2‑139 (MQ=255)
                  gaccGATTGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCg                                                                                                               <  1:430312/136‑1 (MQ=255)
                        ttGTCAACCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCa                                                                                                         <  1:371256/139‑1 (MQ=255)
                             aaCCGCATCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATgggg                                                                                                    >  1:236179/1‑137 (MQ=255)
                                  catCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCGCGt                                                                                                              <  3:196149/122‑1 (MQ=255)
                                  catCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGt                                                                                                              >  4:196149/3‑124 (MQ=255)
                                   atCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGc                                                                                              <  4:168307/138‑1 (MQ=255)
                                   atCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGc                                                                                              <  7:116599/138‑1 (MQ=255)
                                              ttCTGAACTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTtct                                                                                    >  2:9673/1‑138 (MQ=255)
                                                   aaCTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTtctcag                                                                                 <  2:236179/136‑3 (MQ=255)
                                                   aaCTGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTcagc                                                                                <  4:259374/137‑1 (MQ=255)
                                                      tGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGa                                                                                                                               >  7:227461/1‑87 (MQ=255)
                                                      tGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGa                                                                                                                               <  8:227461/87‑1 (MQ=255)
                                                      tGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCGGTTTTTTGTCAGCGGCtt                                                                           >  6:297602/1‑139 (MQ=255)
                                                      tGCGCGGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTGGCTGTTTTTTCTCAGCGGAtt                                                                           >  1:403778/1‑139 (MQ=255)
                                                        cgcgGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGa                                                                                                                               <  8:150899/85‑1 (MQ=255)
                                                        cgcgGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGa                                                                                                                               >  7:150899/1‑85 (MQ=255)
                                                        cgcgGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGa                                                                                                                               <  4:407283/85‑1 (MQ=255)
                                                        cgcgGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGa                                                                                                                               >  3:407283/1‑85 (MQ=255)
                                                        cgcgGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTCAGCGGAtttt                                                                         >  6:268382/1‑139 (MQ=255)
                                                        cgcgGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCACAGGGGAtttt                                                                         >  2:208294/1‑139 (MQ=255)
                                                        cgcgGCTGCTCTGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATCGGGTCTTGCTGTGTTTTCTCAGAGGAttt                                                                          >  5:53142/1‑138 (MQ=255)
                                                                ctctGAGTTTTTGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTCAGCGGATTTTAATGttt                                                                  <  2:34445/138‑1 (MQ=255)
                                                                     agtttttGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGCATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTGTTTTCTGAGCGGATTTTAATGTTTTTTcc                                                             >  5:405256/4‑137 (MQ=255)
                                                                          ttGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTcagcg                                                                               >  5:124920/1‑115 (MQ=255)
                                                                          ttGTGTGTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTcagcg                                                                               <  6:124920/115‑1 (MQ=255)
                                                                             tgtgTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTCAGCGGATTTTAATGTTTTTTCAAAATactcc                                                    <  6:428582/139‑5 (MQ=255)
                                                                             tgtgTCTGATGTATCAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTCAGCGGATTTTAATGTTTTTTCAAAATactcc                                                    <  1:308261/139‑5 (MQ=255)
                                                                                         atcatcTTGCTTTAAACTCCGAACCATATCATTATCGCCAAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTGGCTGTTTTTTCTCAGCGGATTTTAATGTTTTTTCAAAATACTCCAAGACTTGAAg                                         <  2:259802/138‑1 (MQ=255)
                                                                                           cAGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTCAGCGGATTTTAATGTTTTTTCAAAATACTCCAAGACTTGAAgcgc                                      >  6:419772/1‑139 (MQ=255)
                                                                                            aGCTTGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTCAGCGGATTTTAATGTTTTTTCAAAATACTCCAAGACTTGAAGCGcc                                     <  7:52651/139‑1 (MQ=255)
                                                                                               ttGCTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTCAGCGGATTTTAATGTTTTTTCAAAATACTCCAAGACTTGAAGCGCctct                                  >  5:87875/2‑139 (MQ=255)
                                                                                                  ctatAAACTACGAACCATAAAATTATAGCCCAATGTGATTTTACGAAATAGGGCTCCCGTTAGCATGGGGTCTAGGTGTTTTTTCTAAGCGGATTTTAATGTTTTTTAAAAATACTACAAGACATCAAGCGCCTCTTc                                >  6:155073/4‑138 (MQ=255)
                                                                                                  cTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTggg                                                                                                                     >  7:415683/1‑53 (MQ=255)
                                                                                                  cTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTggg                                                                                                                     <  8:415683/53‑1 (MQ=255)
                                                                                                  cTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGc                                                                                              <  4:353814/76‑1 (MQ=255)
                                                                                                  cTTTAAACTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGc                                                                                              >  3:353814/1‑76 (MQ=255)
                                                                                                       aaCTCCGAACCATATCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTCAGCGGATTTTAATGTTTTTTCAAAATACTCCAAGACTTGAAGCGCCTCTTCATAttt                          >  7:139053/1‑139 (MQ=255)
                                                                                                       aaCTCCGAACCATATCATAAAAGCCCAATGTGATTTGACCAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTCAGCGGATTTTAATGTTTTTTCAAAATACTCCAAGACTTGAAGCGCCTCTTCATAttt                          <  2:57031/139‑1 (MQ=255)
                                                                                                                   tatCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTCAGCGGATTTTAATGTTTTTTCAAAATACTCCAAGACTTGAAGCGCCTCTTCATATTTCGCCTGTCGGGt              >  4:341467/1‑139 (MQ=255)
                                                                                                                    atCATTATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTCAGCGGATTTTAATGTTTTTTCAAAATACTCCAAGACTTGAAGCGCCTCTTCATATTTCGCCTGTCGGGt              >  2:388782/1‑138 (MQ=255)
                                                                                                                     tCATTATAGCCCATTGGTATTTGACGATTTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTATCTGGGGTTTTTAATGTTTTTTCAAAATACTCCAATACTTCAAGCGCCTCTTCATATTTCGCCTGTCGGGTc             <  4:20994/138‑1 (MQ=255)
                                                                                                                        ttATAGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTCAGCGGATTTTAATGTTTTTTCAAAATACTCCAAGACTTGAAGCGCCTCTTCATATTTCGCCTGTCgggggagtt         >  1:218537/1‑133 (MQ=255)
                                                                                                                          ataGCCCTATGTGTATTGCCTAATTGGGGCACCCTTTAGCTTGGGGTATTGCTGTTTTTTCTCAGCGTTTTTAAATGTTTTTTCAAAAAACTCCAAGACTTGCAGCGCCTCTTCATATTTCGCCTGTCGGGTCATTTg        <  1:208294/138‑1 (MQ=255)
                                                                                                                           tcgCCCAATGTTATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTCAGCGGATTTTAATGTTTTTAAAAAATACTCCACGACTTGACGCGCCTCTTCATATTTCGCCTGTCGGGTCATTTGa       <  5:297602/136‑1 (MQ=255)
                                                                                                                           taGCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTCAGCGGATTTTAATGTTTTTTCAAAATACTCCAAGACTTGAAGCGCCTCTTCATATTTCGCCTGTCGGGTCATTTGaa      <  8:320139/139‑1 (MQ=255)
                                                                                                                             gCCCAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTCAGCGGATTTTAATGTTTTTTCAAAATACTCCAAGACTTGAAGCGCCTCTTCATATTTCGCCTGTCGGGTCATTTGa       >  6:147956/1‑136 (MQ=255)
                                                                                                                              cccAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGc                                                                                              >  5:248442/1‑48 (MQ=255)
                                                                                                                              cccAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGc                                                                                              <  6:248442/48‑1 (MQ=255)
                                                                                                                              cccAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTCAGCGGATTTTAATGTTTTTTCAAAATACTCCAAGACTTGAAGCGCCTCTTCATATTTCGCCTGTCGGGTc             >  1:314215/1‑129 (MQ=255)
                                                                                                                              cccAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTTTTTTCTCAGCGGATTTTAATGTTTTTTCAAAATACTCCAAGACTTGAAGCGCCTCTTCATATTTCGCCTGTCGGGTCATTTGAAAGa   >  2:23871/1‑139 (MQ=255)
                                                                                                                              cccAATGTGATTTGACGAATTTGGGCTCCCGTTAGCATGGGGTCTTGCTGTGTTTTCTCAGCGGATTTTAATGTTTTTTCAAAATACTCCAAGACTTGAAGCGCCTCTTCATATTTCGCCTGTCGGGGCATTTGAAAGa   <  1:238863/139‑1 (MQ=255)
                                                                                                                              cccAATGTGATTTGACGAATTTGGGCTCCCGTTAGCAGGGGGTCTTGCTGTTTTTTCTCAGCGGATTTTAATGTTTTTTCAAAATACTCCAAGACTTGAAGCGCCTCTTCATATTTCGCCTGTCGGGTc             <  2:314215/129‑1 (MQ=255)
                                                                                                                              cccAATGTGATTTGACGAATTTGGGCTCCCGTTAGCAGGGGGGCTTGCTGTTTTTTCTCAGCGGATTTTAATGTTTTTTCAAAATACTCCAAGACTTGAAGCGCCTCTTCATATTTCGCCTGTCGGGTCATTTGAAAGa   <  6:205336/139‑1 (MQ=255)
                                                                                                                               ccAATGTGATTTGCCGAATTTGGGCTCCCGTTAGCATGGGGTCTTGGTGTTTTTTCTCCGCGGTTTTTAATGTTTTTTCAAAATACTCCAAGACTTGAAGCGCCTCTTCATATTTCGCCTGTCGGGTCATTTGAAAGAc  <  5:268382/139‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                       
GAGCCCCAAAGCGGATCAGATCGATTGTCAACTGCGTCCATCAGCATTCTGAACTGCGCGGCTGCTCTGAGCTTTTGTGTATCTGATGTATCAGCCTGCTTTAAACTCCGTACCATATCATTATAGCCCAGTGTGATTTGACGGATTTGGGCTCCCGTTAGCATGGTGTCTTGCTTTTTTTTCTCGGCGGATTTTAATGTTTTTTCAAAATAAGCAAGGACTTGCAGCGCCTCTTCATATTTTGACTGGCGGGTCATTTGAAAGAC  >  CP006881/2179809‑2180074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑