Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,180,425 C→T T141T (ACG→ACA)  U712_10915 ← Uncharacterized protein ypjA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,180,4250CT98.9% 263.9 / ‑5.0 87T141T (ACG→ACA) U712_10915Uncharacterized protein ypjA
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (47/39);  total (48/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

GAGAAAGTAAGCAAGCCCAATTGAAAAAATACTCAGCCAAAATGTTCCATACCCTATATCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATGTCAAACAAGTAATCAATGACATCGTTGTGCAGCGTCCACACTGCAGCAATTGCAAGATGCCAAAAAGAAAAACGAAAATACGGACTGTACAACACGCCTTGGACTGCCATGGCAAAATGCGAGGCAATCAGCATATAGCCCTCCCACGGCAAGTTACCTTC  >  CP006881/2180293‑2180553
                                                                                                                                    |                                                                                                                                
aagaAAGTAAGCAAGCGCGATTGAAAAGATACTTAGCCAAAATGTTCCGTATCCTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTc                                                                                                                               >  6:108570/2‑136 (MQ=255)
       tAAGCAAGCGCGATTGAAAAGATACTTAGCCAAAATGTTCCGTATCCTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTgcag                                                                                                                     >  2:88286/1‑139 (MQ=255)
        aagcaagcGCGATTGAAAAGATACTTAGCCAAAATGTTCCGTATCCTATTTCAGTCATATAATCAGAATGCATAGAGTATTGCGGCATCATATCAAACTAGTAGTCAATGAGATCGTTATGCAGTGTCCAGACTgcag                                                                                                                     >  2:380953/1‑138 (MQ=255)
         agcaagcGCGATTGAAAAGATACTTAGCCAAAATGTTCCGTATCCTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTgcagc                                                                                                                    <  4:333456/138‑1 (MQ=255)
         agcaagcGCGATTGAAAAGATACTTAGCCAAAATGTTCCGTATCCTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTgcagc                                                                                                                    >  5:394451/1‑138 (MQ=255)
          gcaagcGCGATTGAAAAGATACTTAGCCAAAATGTTCCGTATCCTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTc                                                                                                                               >  1:145168/1‑126 (MQ=255)
          gcaagcGCGATTGAAAAGATACTTAGCCAAAATGTTCCGTATCCTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTc                                                                                                                               <  2:145168/126‑1 (MQ=255)
          gcaagcGCGATTGAAAAGATACTTAGCCAAAATGTTCCGTATCCTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCaa                                                                                                                  >  4:116011/1‑139 (MQ=255)
          gcaagcGCGATTGAAAAGATACTTAGCCAAAATGTTCCGTATCCTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCaa                                                                                                                  >  7:59244/1‑139 (MQ=255)
              gcgcgaTTGAAAAGATACTTAGCCAAAATGTTCCGTATCCTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCGGTGTCCAGACTGCAGCAATTg                                                                                                               >  1:289971/6‑138 (MQ=255)
               cgcgaTTGAAAAGATACTTAGCCAAAATGTTCCGTATCCTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGc                                                                                                              >  1:322403/5‑138 (MQ=255)
                 cgaTTGAAAAGATACTTAGCCAAAATGTTCCGTATCCTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGc                                                                                                              >  8:194600/3‑136 (MQ=255)
                              aCTTAGCCAAAATGTTCCGTATCCTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGa                                                                                               >  5:387086/1‑138 (MQ=255)
                                 taGCCAAAATGTTCCGTATCCTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGaaaaa                                                                                           >  1:51703/2‑139 (MQ=255)
                                   gCCAAAATGTTCCGTATCCTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAAc                                                                                          >  8:300497/1‑138 (MQ=255)
                                    ccAAAATGTTCCGTATCCTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACg                                                                                         >  8:346870/1‑138 (MQ=255)
                                            ttCCGTATCCTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACgg                                                                                <  7:292259/139‑1 (MQ=255)
                                            ttCCGTATCCTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACg                                                                                 >  6:185042/1‑138 (MQ=255)
                                              ccgtatccTATTTGAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGa                                                                               <  5:219300/132‑1 (MQ=255)
                                              ccgtatccTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCGGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGa                                                                               <  2:289971/132‑1 (MQ=255)
                                              ccgtatccTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGa                                                                               <  2:322403/132‑1 (MQ=255)
                                              ccgtatccTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGa                                                                               <  6:387086/132‑1 (MQ=255)
                                              ccgtatccTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGa                                                                               <  7:194600/132‑1 (MQ=255)
                                              ccgtatccTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGa                                                                               <  7:346870/132‑1 (MQ=255)
                                              ccgtatccTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGa                                                                               <  5:429551/132‑1 (MQ=255)
                                              ccgtatccTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGa                                                                               <  5:108570/132‑1 (MQ=255)
                                              ccgtatccTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGGCCAGCCTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGa                                                                               <  2:283403/132‑1 (MQ=255)
                                              ccgtatccTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTAAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGa                                                                               <  1:149323/132‑1 (MQ=255)
                                              ccgtatccTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAAAAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGa                                                                               <  2:369618/132‑1 (MQ=255)
                                              ccgtatccTATTTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCAAATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGa                                                                               <  5:103826/132‑1 (MQ=255)
                                              ccgtatccTATTTCAGTAATATAATCAGAAAGCATATAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGAAGCAATTGCAAGATGCCAAAATTAAAAACGAAAATACGGa                                                                               <  6:285325/132‑1 (MQ=255)
                                                      tattTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACg                                                                                         >  3:126981/1‑120 (MQ=255)
                                                      tattTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACg                                                                                         <  4:126981/120‑1 (MQ=255)
                                                      tattTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACg                                                                                 >  7:443918/1‑128 (MQ=255)
                                                      tattTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACg                                                                                 <  8:443918/128‑1 (MQ=255)
                                                      tattTCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTataat                                                                      >  7:40816/1‑138 (MQ=255)
                                                        tttCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACg                                                                                         >  3:138202/3‑118 (MQ=255)
                                                        tttCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACg                                                                                         <  4:138202/116‑1 (MQ=255)
                                                        tttCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTataat                                                                      <  8:59244/135‑2 (MQ=255)
                                                         ttCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACg                                                                   >  8:110651/2‑139 (MQ=255)
                                                         ttCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACg                                                                   >  2:26202/2‑139 (MQ=255)
                                                                  ataATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTGAACt                                                          >  4:197706/1‑139 (MQ=255)
                                                                  ataATCAGAAAGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTGAACt                                                          >  7:116174/1‑139 (MQ=255)
                                                                          aaaGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTGAACTGCCAttg                                                   >  3:402931/1‑136 (MQ=255)
                                                                          aaaGCATAGAGTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTGAACTGCCAttg                                                   >  6:382934/1‑136 (MQ=255)
                                                                                  gagTATTGCGGCATCATATCAAACAAGTAGTCAATGACCTCGTTCTGCAGTGTCCAGACTGCAGCAATTGCAATATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTGAACTGCCATTGCAAAATGCg                                          <  7:98128/139‑1 (MQ=255)
                                                                                    gTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGa                                                                               <  4:262305/100‑1 (MQ=255)
                                                                                    gTATTGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGa                                                                               >  3:262305/1‑100 (MQ=255)
                                                                                       ttGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGa                                                                               <  4:18448/97‑1 (MQ=255)
                                                                                       ttGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGa                                                                               >  3:18448/1‑97 (MQ=255)
                                                                                        tGCGGCATCATGTCAAACAAGTAATCAATGACATCGTTGTGCAGCGTCCACACTGCAGCAATTGCAAGATGCCAAAAAGAAAAACGAAAATACGGACTGTACAACACGCCTTGGACTGCCATGGCAAAATGCGAGGCaa                                    >  5:214630/1‑139 (MQ=255)
                                                                                        tGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGa                                                                               >  2:47305/1‑96 (MQ=255)
                                                                                        tGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGa                                                                               >  1:311742/1‑96 (MQ=255)
                                                                                        tGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGa                                                                               <  1:47305/96‑1 (MQ=255)
                                                                                        tGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGa                                                                               <  2:311742/96‑1 (MQ=255)
                                                                                        tGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACt                                                                             >  2:15617/1‑98 (MQ=255)
                                                                                        tGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACt                                                                             <  1:15617/98‑1 (MQ=255)
                                                                                        tGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTGAACTGCCATTGCAAATTGCGaagaaa                                    >  5:335802/1‑134 (MQ=255)
                                                                                        tGCGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTGAACTGCCATTGCAAAATGCGAAGCaa                                    >  1:244616/1‑139 (MQ=255)
                                                                                        tGCGGCATCATATCAAACAAGAAGTCAATGACATCGGTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTGAACTGCCATTGCAAAATGCGAAGCaa                                    >  2:97810/1‑139 (MQ=255)
                                                                                          cGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTGAACTGCCATTGCAAAATGCGAAGCAAtt                                  <  5:382934/139‑2 (MQ=255)
                                                                                          cGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTGAACTGCCATTGCAAAATGCGAAGCAAt                                   <  3:143547/138‑1 (MQ=255)
                                                                                          cGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTGAACTGCCATTGCAAAATGCGAAGCAAt                                   <  5:398520/138‑1 (MQ=255)
                                                                                          cGGCATCATATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTGAACTGCCATTGCAAAATGCGAAGCAAt                                   <  1:97810/138‑1 (MQ=255)
                                                                                             catcatATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGa                                                                               >  4:282378/1‑91 (MQ=255)
                                                                                             catcatATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGa                                                                               <  3:282378/91‑1 (MQ=255)
                                                                                             catcatATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTGAACTGCCATTGCAAAATGCGAAGCAATTAg                                >  5:287637/1‑138 (MQ=255)
                                                                                             catcatATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTGAACTGCCATTGCAAAATGCGAAGCAATTAg                                >  3:109635/1‑138 (MQ=255)
                                                                                               tcatATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTga                                                             <  3:293348/107‑2 (MQ=255)
                                                                                               tcatATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTga                                                             >  4:293348/1‑106 (MQ=255)
                                                                                               tcatATCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCAAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTGAACTGCCATTGCAAAATGCGAAGCAATTAGCat                             >  2:176275/1‑139 (MQ=255)
                                                                                                  tatCAAACAAGTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTGAACTGCCATTGCAAAATGCGAAGCAATTAGCatg                            >  6:187568/3‑136 (MQ=255)
                                                                                                            gTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACg                                                                                 <  2:101804/74‑1 (MQ=255)
                                                                                                            gTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACg                                                                                 >  1:101804/1‑74 (MQ=255)
                                                                                                            gTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGa                                                                               >  4:73615/1‑76 (MQ=255)
                                                                                                            gTAGTCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGa                                                                               <  3:73615/76‑1 (MQ=255)
                                                                                                                tCAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTGAACTGCCATTGCAAAATGCGAAGCAATTAGCATGTAGCCCTCCCACGGc             >  1:399659/1‑138 (MQ=255)
                                                                                                                 cAATGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTGAACTGCCATTGCAAAATGCGAAGCAATTAGCATGTAGCCCTCCCACGGCa            <  8:116174/138‑1 (MQ=255)
                                                                                                                    tGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGa                                                                               >  2:292904/1‑68 (MQ=255)
                                                                                                                    tGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTGAACTGCCATTGCAAAATGCGAAGCAATTAGCATGTAGCCCTCCCACGGCAAggc        >  7:143580/1‑137 (MQ=255)
                                                                                                                    tGACATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCAAAAATGAAAAACGAAAATACGGa                                                                               <  1:292904/68‑1 (MQ=255)
                                                                                                                      aCATCGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTGAACTGCCATTGCAAAATGCGAAGCAATTAGCATGTAGCCCTCCCACGGCAAGTCAc      <  3:197706/139‑1 (MQ=255)
                                                                                                                      aCATAGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTGAACTGCCATTGCAAAATGCGAAGCAATTAGCATGTAGCCCTCCCACGGCAAGtca       <  8:289332/138‑3 (MQ=255)
                                                                                                                          cGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTGAACTGCCATTGCAAAATGCGAAGCAATTAGCATGTAGCCCTCCCACGGCa            >  6:270501/1‑129 (MQ=255)
                                                                                                                          cGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTGAACTGCCATTGCAAAATGCGAAGCAATTAGCATGTAGCCCTCCCACGGCa            <  5:270501/129‑1 (MQ=255)
                                                                                                                          cGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTGAACTGCCATTGCAAAATGCGAAGCAATTAGCATGTAGCCCTCCCACGGCAAGTCAccagt  >  5:389113/1‑136 (MQ=255)
                                                                                                                          cGTTATGCAGTGTCCAGACTGCAGCAATTGCAAGATGCCAAAATGAAAAACGAAAATACGGACTGTATAATACGCCTTGAACTGCCATTGCAAAATGCGAAGCAATTAGCAGGTAGCCCTCCCACGGCAAGTCAccagt  >  4:43811/1‑136 (MQ=255)
                                                                                                                                    |                                                                                                                                
GAGAAAGTAAGCAAGCCCAATTGAAAAAATACTCAGCCAAAATGTTCCATACCCTATATCAGTCATATAATCAGAAAGCATAGAGTATTGCGGCATCATGTCAAACAAGTAATCAATGACATCGTTGTGCAGCGTCCACACTGCAGCAATTGCAAGATGCCAAAAAGAAAAACGAAAATACGGACTGTACAACACGCCTTGGACTGCCATGGCAAAATGCGAGGCAATCAGCATATAGCCCTCCCACGGCAAGTTACCTTC  >  CP006881/2180293‑2180553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑