Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,172,319 A→G A69A (GCT→GCC)  U712_10860 ← 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,172,3190AG95.7% 208.5 / ‑0.7 71A69A (GCT→GCC) U712_108603‑methyl‑2‑oxobutanoate hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base G (43/24);  total (45/26)
Fisher's exact test for biased strand distribution p-value = 5.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

CTCAAGCTTCAGTGCGTCAGCTCCGCTTTCCTGAACGATAACTGCTGCATTTTTCAGCGTATCCTCCTTGGACAGGTGATAAGACATAAACGGCATGTCTGTCACAATAAAAGTATTCTGCGCGCCCCTTTTAACAGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCGAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGCTTAGCTGCCGGA  >  CP006881/2172184‑2172451
                                                                                                                                       |                                                                                                                                    
cTCAAGCTTCAGTGCGTCAGCTCCGCTTTCCTGAACGATAGCCGCTGCATTTTTCAGCGTATCTTCCTTAGACAGGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCt                                                                                                                                   >  3:76920/1‑139 (MQ=255)
   aaGCTTCAGTGCGTCAGCTCCGCTTTCCTGAACGATAGCCGCTGCATTTTTCAGCGTATCTTCCTTAGACAGGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCtttt                                                                                                                                <  6:171692/139‑1 (MQ=255)
         cAGTGCGTCAGCTCCGCTTTCCTGAACGATAGCCGCTGCATTTTTCAGCGTATCTTCCTTAGACAGGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTatga                                                                                                                          >  3:204112/1‑139 (MQ=255)
          aGTGCGTCAGCTCCGCTTTCCTGAACGATAGCCGCTGCATTTTTCAGCGTATCTTCCTTAGACAGGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTatga                                                                                                                          <  3:104008/138‑1 (MQ=255)
           gTGCGTCAGCTCCGCTTTCCTGAACGATAGCCGCTGCATTTTTCAGCGTATCTTCCTTAGACAGGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCtttt                                                                                                                                >  1:38903/1‑131 (MQ=255)
           gTGCGTCAGCTCCGCTTTCCTGAACGATAGCCGCTGCATTTTTCAGCGTATCTTCCTTAGACAGGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCtttt                                                                                                                                <  2:38903/131‑1 (MQ=255)
               ggcAGCTCCGCTTTCCTGAACGATAGCGGCTGCATTTTTCAGCGTATCTTCCTTAGACAGGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATc                                                                                                                    <  3:66028/137‑1 (MQ=255)
               gTCAGCTCCGCTTTCCTGAATGATAGCCGCTGCATTTTTCAGCGTATCTTCCTTAGACAGGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATc                                                                                                                    <  3:409621/139‑1 (MQ=255)
                tCAGCTCCGCTTTCCTGAACGATAGCCGCTGCATTTTTCAGCGTATCTTCCTTAGACAGGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCa                                                                                                                   >  2:312644/1‑139 (MQ=255)
                      ccGCTTTCCTGAACGATAGCCGCTGCATTTTTCAGCGTATCTTCCTTAGACATGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCg                                                                                                             >  2:142799/1‑139 (MQ=255)
                       cGCTTTCCTGAACGATAGCCGCTGCATTTTTCAGCGTATCTTCCTTAGACAGGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGc                                                                                                            <  7:368801/139‑1 (MQ=255)
                       cGCTTTCCTGAACGATAGCCGCTGCATTTTTCAGCGTATCTTCCTTAGACAGGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGc                                                                                                            <  4:295495/139‑1 (MQ=255)
                       cGCTTTCCTGAACGATAGCCGCTGCATTTTTCAGCGTATCTTCCTTAGACAGGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGc                                                                                                            <  8:319107/139‑1 (MQ=255)
                         cTTTCCTGAACGATAGCCGCTGCATTTTTCAGCGTATCTTCCTTAGACAGGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCaa                                                                                                          >  8:376015/1‑139 (MQ=255)
                              cTGAACGATAGCCGCTGCATTTTTCAGCGTATCTTCCTTAGACAGGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGt                                                                                                      >  6:23278/1‑138 (MQ=255)
                              cTGAACGATAGCCGCTGCATTTTTCAGCGTATCTTCCTTAGACAGGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGt                                                                                                      <  4:76920/138‑1 (MQ=255)
                                     atagccgctgcATTTTTCAGCGTATCTTCCTTAGACAGGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGa                                                                                              <  6:174789/133‑1 (MQ=255)
                                      tagccgctgcATTTTTCAGCGTATCTTCCTTAGACAGGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGa                                                                                              >  6:32817/6‑138 (MQ=255)
                                      tagccgctgcATTTTTCAGCGTATCTTCCTTAGACAGGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGa                                                                                              >  4:113887/6‑138 (MQ=255)
                                      tagccgctgcATTTTTCAGCGTATCTTCCTTAGACAGGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGa                                                                                              >  5:63110/6‑138 (MQ=255)
                                        gccgctgcATTTTTCAGCGTATCTTCCTTAGACAGGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACCCCGACAg                                                                                           >  7:58710/4‑139 (MQ=255)
                                         ccgctgcATTTTTCAGCGTATCTTCCTTAGACAGTTGATAAGACAAAAACGTCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGt                                                                                          <  8:436503/137‑1 (MQ=255)
                                         ccgctgcATTTTTCAGCGTATCTTCCTTAGACAGGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGt                                                                                          <  3:113887/137‑1 (MQ=255)
                                                        gCGTATCTTCCTTAGACAGGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAg                                                                           <  5:32817/139‑1 (MQ=255)
                                                             acatcctTAGACAGGTCATAAGACATACACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGgacga                                                                      <  6:328960/136‑1 (MQ=255)
                                                                   ttagACAGGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAGCGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGCCAGTTGCATCAAGGCCGAGGCCGACCATTc                                                                >  4:348644/4‑139 (MQ=255)
                                                                     agACAGGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTACCGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTcc                                                               >  8:63980/2‑138 (MQ=255)
                                                                     agACAGGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTcc                                                               >  8:70844/2‑138 (MQ=255)
                                                                       aCAGGTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAg                                                            >  5:329422/1‑139 (MQ=255)
                                                                           gagATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACACCGTTTAACGGCTTTTGTATGAGGGAGCATGTCCGCAACTGTAACACCGACAGATGAATCAATGACGAGGACGACCCTTCCAAGTGaa                                                        >  8:339179/3‑139 (MQ=255)
                                                                           gTGATAAGACATAAACGGCATATCTGTCACAATATAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAGTGaa                                                        >  8:203182/1‑139 (MQ=255)
                                                                           gTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAATGCCGAGGACGACCATTCCAAGTGaa                                                        >  7:7503/1‑139 (MQ=255)
                                                                           gTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAGTgca                                                        >  7:106499/1‑137 (MQ=255)
                                                                           gTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAGTGaa                                                        >  3:154241/1‑139 (MQ=255)
                                                                           gTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAGTGaa                                                        >  7:121210/1‑139 (MQ=255)
                                                                           gTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAGTGaa                                                        >  7:393081/1‑139 (MQ=255)
                                                                           gTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAGTGaa                                                        >  8:387543/1‑139 (MQ=255)
                                                                           gTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGACCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAGTGaa                                                        >  1:391919/1‑139 (MQ=255)
                                                                           gTGATAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACCGCTTTTGTATGATGGATCATGTCCGCAACTGACACACCGACAGTTGAATCAAGTCCGAGGACGACCATTCCAAGTGa                                                         >  4:404433/1‑138 (MQ=255)
                                                                               tAAGACATAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAGTGAATCa                                                     <  8:393081/138‑1 (MQ=255)
                                                                                     aTAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCCCCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAGTGAATCACCGACt                                               >  2:407310/1‑138 (MQ=255)
                                                                                     aTAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGc                                                                                                            <  4:91949/77‑1 (MQ=255)
                                                                                     aTAAACGGCATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGc                                                                                                            >  3:91949/1‑77 (MQ=255)
                                                                                             cATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAGTCAAGGCCGAGGACGACCATTCCAAGTGAATCACCGACTAAAATCATg                                      >  7:94083/1‑139 (MQ=255)
                                                                                             cATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAGTGAATCACCGACTAAAATCATg                                      >  8:80135/1‑139 (MQ=255)
                                                                                             cATATCTGTCACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAGTGAATCACCGACTAAAATCATg                                      >  6:217183/1‑139 (MQ=255)
                                                                                                     tcACAATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAGTGAATCACCGACTAAAATCATGTCAACTcc                              >  7:12747/1‑139 (MQ=255)
                                                                                                       acaATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAgg                                                                                <  4:277019/87‑1 (MQ=255)
                                                                                                       acaATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAgg                                                                                >  3:277019/1‑87 (MQ=255)
                                                                                                       acaATAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAGTGAATCACCGACTAAAATCATGTCAACTCCCg                            >  3:379848/1‑139 (MQ=255)
                                                                                                           tAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGt                                                                                                      >  1:132657/1‑61 (MQ=255)
                                                                                                           tAAAGGTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGt                                                                                                      <  2:132657/61‑1 (MQ=255)
                                                                                                               ggTATTCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCa                    <  4:154241/138‑1 (MQ=255)
                                                                                                                   ttCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGCCCATTCCAAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGc                  >  5:201438/1‑137 (MQ=255)
                                                                                                                   ttCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGc                  >  5:89906/1‑137 (MQ=255)
                                                                                                                   ttCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGc                  >  8:436878/1‑137 (MQ=255)
                                                                                                                   ttCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGc                  >  4:442632/1‑137 (MQ=255)
                                                                                                                   ttCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGc                  >  2:259269/1‑137 (MQ=255)
                                                                                                                   ttCGGCGCACCCCTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAGGGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGc                  >  3:55270/1‑137 (MQ=255)
                                                                                                                   ttCGGCGCACCCCTTTAAACGGCTTTTGTATTATGGATCATTTAAGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCAAAGTGAATCCCCGACTAAAATCATGTCAACTCCCGCTTGTTCAgaa                 >  4:179200/1‑136 (MQ=255)
                                                                                                                   ttCGGCGCACCCCTTATAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGCCCATTCCAAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGc                  >  2:135781/1‑137 (MQ=255)
                                                                                                                           accccTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCt        >  5:98821/2‑139 (MQ=255)
                                                                                                                            ccccTTTTAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTg       <  7:80135/139‑1 (MQ=255)
                                                                                                                             cccTTTTAACAGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCGAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGCTTAg          >  6:86674/1‑135 (MQ=255)
                                                                                                                             cccTTTTAACAGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCGAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGCTTAg          <  5:86674/135‑1 (MQ=255)
                                                                                                                             cccTCTTAACGGCTTATGTATGATGCATCATGGCCGCAACTGTCCCACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGc      <  4:401696/139‑1 (MQ=255)
                                                                                                                              ccTTTTAACAGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCGAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGCTTAGCTGcc     <  2:270325/139‑1 (MQ=255)
                                                                                                                                  ttAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCgg   <  1:259269/137‑1 (MQ=255)
                                                                                                                                  ttAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGa  >  2:275005/1‑138 (MQ=255)
                                                                                                                                  ttAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGa  <  4:204112/138‑1 (MQ=255)
                                                                                                                                  ttAACGGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCAAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGa  <  6:89906/138‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                    
CTCAAGCTTCAGTGCGTCAGCTCCGCTTTCCTGAACGATAACTGCTGCATTTTTCAGCGTATCCTCCTTGGACAGGTGATAAGACATAAACGGCATGTCTGTCACAATAAAAGTATTCTGCGCGCCCCTTTTAACAGCTTTTGTATGATGGATCATGTCCGCAACTGTCACACCGACAGTTGAATCAAGGCCGAGGACGACCATTCCGAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGCTTAGCTGCCGGA  >  CP006881/2172184‑2172451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑