Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,180,788	G→A	I20I (ATC→ATT)	U712_10915 ←	Uncharacterized protein ypjA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,180,788	0	G	A	100.0%	159.3 / NA	51	I20I (ATC→ATT)	U712_10915	Uncharacterized protein ypjA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/26);  total (25/26)

AAAGGCGAGCAGCACAAACAGAAAGAAAAACGTTGCTGTCGGACTATCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAGTAGCTGCGGCAAGTACCAATAGTATCCGTATACCGTTCCCAGAAAATTGATGGCTAAGACAAGAATAAGCATCGGCTGCCGTCCTAATACATATTGAAACCATTTCACATCAAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTT  >  CP006881/2180656‑2180914                                                                                                                                     |                                                                                                                               aaaGGCAAGCAGCACAAACAGAAAGAAAAACGTTGCTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGc                                                                                                                           >  2:95195/1‑138 (MQ=255)      caagcagcACAAACAGAAAGAAAAACGTTGCTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGCCTAAGa                                                                                                                      >  3:46134/3‑138 (MQ=255)          cagcaCAAACAGAAAGAAAAACGTTGCTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAg                                                                                                                  >  1:98212/1‑138 (MQ=255)                  cctaaaagaatAAGGTGGGTGGCGGACTGTCCGGAACAAAAAGACGGAAACGGGCCGGTGTCTCCAAATGCTGCGGCAAGTACAAATAGTAGCCGTAAACCGTTCCCAGAAATTTAATGGCTAAGACAAGAATAAGCAt                                                                                                         <  2:293988/135‑1 (MQ=255)                       aataaaacCGTTGCTGTCGCACTGGCCGTAACAAAAATCTGCAACCGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGcc                                                                                                    <  4:112741/136‑2 (MQ=255)                       aagaaaAACGTTGCTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGcc                                                                                                    >  1:406238/1‑138 (MQ=255)                         taaaacCGTTGCTGGCGGCCTGTCCGCAACAAAAAGCAGGAACCGGGCCGGTGGCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGc                                                                                                  <  8:440211/138‑1 (MQ=255)                         gaaaAACGTTGCTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGc                                                                                                  <  7:153073/139‑1 (MQ=255)                         caaacccGTGGCGGTGGGACTGTCCGGAAAAAAAAGCAGCCAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGc                                                                                                  <  7:183128/133‑1 (MQ=255)                         caaaaaCTTTGCGGGCGTCCTGGCCGTAAAAAAAAGCCGCACACGGGCCGGGGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGc                                                                                                  <  3:43811/138‑1 (MQ=255)                         caaaaaCGTTGCTGTGGGACTGTCCGGAACAAAAATCACGAACCGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCTATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGc                                                                                                  <  3:163374/138‑1 (MQ=255)                                   gCTGGCGGACGGTCCGGAACAAAAATCAGGAACCGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATa                                                                                        <  4:182062/139‑1 (MQ=255)                                    cTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATa                                                                                        <  5:197631/138‑1 (MQ=255)                                    cTGTCGGACTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATa                                                                                        >  5:123287/1‑138 (MQ=255)                                           aCTGTCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGa                                                                                <  3:317391/139‑1 (MQ=255)                                            cttaccGAAACAAAAAACAGCAAACGGGCCTGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTA‑ACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGaaa                                                                              <  5:435297/135‑1 (MQ=255)                                               tCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATGAGCATCGGCCGCTGCCCTAATACATATTGAAAc                                                                             >  7:189548/1‑138 (MQ=255)                                                 cGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTATCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCAt                                                                          >  3:384012/1‑139 (MQ=255)                                                  ggAAAAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCAtt                                                                         <  4:367734/139‑1 (MQ=255)                                                   taaCAAAAATCTGCAACCGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCTTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCAttt                                                                        <  7:52047/138‑1 (MQ=255)                                                    aaCAAAAATCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCAttt                                                                        >  1:4007/1‑138 (MQ=255)                                                        aaacccAGTAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCAGTAAACCGTTCCCATAATATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACat                                                                   <  8:233786/134‑1 (MQ=255)                                                        aaacTCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACat                                                                   <  1:95195/139‑1 (MQ=255)                                                         aaaTCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGa                                                                                                                      <  1:100580/87‑1 (MQ=255)                                                         aaaTCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGa                                                                                                                      >  2:100580/1‑87 (MQ=255)                                                         aaaTCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGc                                                                                                  <  6:223284/107‑1 (MQ=255)                                                         aaaTCAGGAAACGGGCCGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGc                                                                                                  >  5:223284/1‑107 (MQ=255)                                                         aaaTCAGGAAACGGGCCAGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGc                                                                                                  >  1:54769/1‑107 (MQ=255)                                                         aaaTCAGGAAACGGGCCAGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGc                                                                                                  <  2:54769/107‑1 (MQ=255)                                                                         cGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTc                                                  <  4:180053/139‑1 (MQ=255)                                                                         cGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTc                                                  <  6:123287/139‑1 (MQ=255)                                                                             gtCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTa                                              <  7:171985/139‑1 (MQ=255)                                                                               ctcCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTaaa                                            <  5:187568/139‑1 (MQ=255)                                                                                  caaaaGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATGAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTaaaa                                           <  8:189548/133‑1 (MQ=255)                                                                                    aaaGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAaataa                                       <  2:98212/137‑1 (MQ=255)                                                                                      aGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAataata                                     >  4:213549/1‑139 (MQ=255)                                                                                          gCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGAc                                                                                                                     >  4:24779/1‑55 (MQ=255)                                                                                          gCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGAc                                                                                                                     <  3:24779/55‑1 (MQ=255)                                                                                                aGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCatat                            >  1:411051/1‑138 (MQ=255)                                                                                                       aTAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGc                    >  6:198439/1‑139 (MQ=255)                                                                                                            agccGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACg               >  3:221755/3‑139 (MQ=255)                                                                                                            agccGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACg               >  7:22618/3‑139 (MQ=255)                                                                                                            agccGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACg               >  8:15046/3‑139 (MQ=255)                                                                                                            agccGTAAACCGCTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGc                                                                                                  <  1:242481/54‑1 (MQ=255)                                                                                                            agccGTAAACCGCTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGc                                                                                                  >  2:242481/3‑56 (MQ=255)                                                                                                                  aaaCCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGt         >  2:400597/3‑139 (MQ=255)                                                                                                                  aaaCCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGt         >  8:265483/3‑139 (MQ=255)                                                                                                                   aaCCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGt         >  8:267452/2‑138 (MQ=255)                                                                                                                        ttCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCtt   >  2:240578/1‑139 (MQ=255)                                                                                                                        ttCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCtt   >  6:216266/1‑139 (MQ=255)                                                                                                                         tCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCttt  >  4:282989/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                               AAAGGCGAGCAGCACAAACAGAAAGAAAAACGTTGCTGTCGGACTATCCGGAACAAAAATCAGGAAACGGGCCGGTGTCTCCAGTAGCTGCGGCAAGTACCAATAGTATCCGTATACCGTTCCCAGAAAATTGATGGCTAAGACAAGAATAAGCATCGGCTGCCGTCCTAATACATATTGAAACCATTTCACATCAAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTT  >  CP006881/2180656‑2180914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑