Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,180,851	A→G	intergenic (‑4/+107)	U712_10915 ← / ← U712_10920	Uncharacterized protein ypjA/Menaquinol‑cytochrome c reductase cytochrome b/c subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,180,851	0	A	G	94.1%	152.8 / ‑5.7	52	intergenic (‑4/+107)	U712_10915/U712_10920	Uncharacterized protein ypjA/Menaquinol‑cytochrome c reductase cytochrome b/c subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (37/11);  minor base C (1/2);  total (38/14)
Fisher's exact test for biased strand distribution p-value = 1.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGTGTCTCCAGTAGCTGCGGCAAGTACCAATAGTATCCGTATACCGTTCCCAGAAAATTGATGGCTAAGACAAGAATAAGCATCGGCTGCCGTCCTAATACATATTGAAACCATTTCACATCAAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAAGTATATCCTTCCATTTTACAATTCACAACACGTTATTTTGCGGTTGTTTCAGAAA  >  CP006881/2180728‑2180980                                                                                                                            |                                                                                                                                  cGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTc                                                                                                                    <  6:123287/139‑1 (MQ=255) cGGTGTCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTc                                                                                                                    <  4:180053/139‑1 (MQ=255)     gtCTCCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTa                                                                                                                <  7:171985/139‑1 (MQ=255)       ctcCAAAAGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTaaa                                                                                                              <  5:187568/139‑1 (MQ=255)          caaaaGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATGAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTaaaa                                                                                                             <  8:189548/133‑1 (MQ=255)            aaaGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAaataa                                                                                                         <  2:98212/137‑1 (MQ=255)              aGCTGCGGCAAGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAataata                                                                                                       >  4:213549/1‑139 (MQ=255)                        aGTACCAATAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCatat                                                                                              >  1:411051/1‑138 (MQ=255)                               aTAGTAGCCGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGc                                                                                      >  6:198439/1‑139 (MQ=255)                                    agccGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACg                                                                                 >  8:15046/3‑139 (MQ=255)                                    agccGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACg                                                                                 >  7:22618/3‑139 (MQ=255)                                    agccGTAAACCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACg                                                                                 >  3:221755/3‑139 (MQ=255)                                          aaaCCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGt                                                                           >  8:265483/3‑139 (MQ=255)                                          aaaCCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGt                                                                           >  2:400597/3‑139 (MQ=255)                                           aaCCGTTCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGt                                                                           >  8:267452/2‑138 (MQ=255)                                                ttCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCtt                                                                     >  2:240578/1‑139 (MQ=255)                                                ttCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCtt                                                                     >  6:216266/1‑139 (MQ=255)                                                 tCCCAGAAAATTAATGGCTAAGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCttt                                                                    >  4:282989/1‑139 (MQ=255)                                                             aaTGGCAAAGAAAATAATAAGCATCGGCCGCTGCCCTAATAAATATTCAAACCATTTCACATCCAATAAAATCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATaaaa                                                         <  2:4007/137‑1 (MQ=255)                                                                ggCTAACACAAGAATAAGCTGCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCCAAACAACTCTCAGTCTGTAAAAAAAATATGTCAATAAAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAAGta                                                      <  8:22618/138‑1 (MQ=255)                                                                    aaGACAATAATACGCAGCGGCCGCTGCCCTAATACATATTCAAACAATTTCACATCGAATCAACTCTCAGTCTGAAAAAATAATATGTCAATATAAAAACGCTGACGCCGGGTCAGCTTTTGATAATAAAAGTATATcc                                                 <  1:240578/139‑1 (MQ=255)                                                                    aaGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAAGTATATcc                                                 >  5:81671/1‑139 (MQ=255)                                                                     aGACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTTTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAAGTATATcc                                                 <  7:267452/138‑1 (MQ=255)                                                                      gACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTc                                                                                                                    >  6:303079/1‑70 (MQ=255)                                                                      gACAAGAATAAGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTc                                                                                                                    <  5:303079/70‑1 (MQ=255)                                                                         aaGAATAACCATCGGCCGCTGCCATAAAAAATATTGAAACCATTTCACATCTAATCAACTCTCTGTCTTTAAAAATAATATGTCAATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAAGTATATCCTTCCa                                            <  6:81671/139‑1 (MQ=255)                                                                                aGCATCGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAAGTATATCCTTCCATTTGAc                                      >  3:160945/1‑138 (MQ=255)                                                                                     cGGCCGCTGCCCTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAAGTATATCCTTCCATTTGACAATTca                                >  5:154380/1‑139 (MQ=255)                                                                                            tgcccTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAAGTATATCCTTCCATTTGACAATTCACAATACg                         >  6:413054/4‑139 (MQ=255)                                                                                              cccTAATACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAAGTATATCCTTCCATTTGACAATTCACAATACg                         <  5:216266/136‑1 (MQ=255)                                                                                                  aaTACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAAGTATATCCTTCCATTTGACAATTCACAATACGTTAttt                   >  8:166452/1‑139 (MQ=255)                                                                                                   aTACATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAAGTATATACTTCACTTTGACAATTCACAATACGTTAtttt                  >  1:353289/1‑139 (MQ=255)                                                                                                      cATATTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAAGTATATCCTTCCATTTGACAATTCACAATACGTTATTTTGCg               >  1:19656/1‑139 (MQ=255)                                                                                                        tatTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAATTATATCCTTCCATTTGACAATTCACAATACGTTATTTTGCGgt             >  1:329247/1‑139 (MQ=255)                                                                                                        tatTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAAGTATATCCTTCCATTTGACAATTCACAATACGTTATTTTGCGgt             >  1:106181/1‑139 (MQ=255)                                                                                                        tatTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAAGTATATCCTTCCATTTGACAATTCACAATACGTTATTTTGCGgt             >  1:429794/1‑139 (MQ=255)                                                                                                        tatTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAAGTATATCCTTCCATTTGACAATTCACAATACGTTATTTTGCGgt             <  2:16812/139‑1 (MQ=255)                                                                                                        tatTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAAGTATATCCTTCCATTTGACAATTCACAATACGTTATTTTGCGgt             >  1:7883/1‑139 (MQ=255)                                                                                                         atTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAAGTATATCCTTCCATTTGACAATTCACAATACGTTATTTTGCGgtt            >  6:209285/1‑139 (MQ=255)                                                                                                         atTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAAGTATATCCTTCCATTTGACAATTCACAATACGTTATTTTGCGgtt            >  4:7530/1‑139 (MQ=255)                                                                                                         atTGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAAGTATATCCTTCCATTTGACAATTCACAATACGTTATTTTGCGgtt            >  3:307080/1‑139 (MQ=255)                                                                                                         atTGAAACCATTTCACATCGAATCAACTCTCAGTCTCTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAAGTATATCCTTCCATTTGACAATTCACAATACGTAATTTTGCgg              >  1:437835/1‑137 (MQ=255)                                                                                                         atTGAAACCATTTCACATCGAATAAACTCTCAGAATGTAAAAATAATATGTCCATATAAAAAACCTGAACCCGGCTCTCCATATGATAATAAAAGAAAATCATTCCATTTGACAATTAACAATACGTTATTTTGCGgtt            >  4:56618/1‑139 (MQ=255)                                                                                                           tGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAATGTATATCATTCCATTTGCCAAATCACAATACGTTATTTTGCGgttgt          >  5:55691/1‑139 (MQ=255)                                                                                                           tGAAACCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCAATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAAGTATATCCTTCCATTTGACAATTCACAATACGTTATTTTGCtgttgt          >  5:347407/1‑139 (MQ=255)                                                                                                             aaaCCATTTCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAAGTATATCCTTCCATTTGACAATTCACAATACGTTATTTTGCGgttgtt         >  3:340785/1‑138 (MQ=255)                                                                                                             aaaCCATTTCACATCCAATCATCTCTCAGTCTGTAAAAATAATATATCCATATAAAAAAGCTGACGCCGGGGCAGCTAGTGATAATAAACGTATTTCCTTCCAGTTGACAATTCACAATACGTTATTATGCgtttgttt        >  8:316444/1‑139 (MQ=255)                                                                                                                   tttCACATCGAATCAACTCTCAGTCTGTAAAACTAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAAGTATATCCTTCCATTTGCCAATTCACCATACGTTATTTTGCGGTTGTTTCAGaaa  >  8:51091/1‑139 (MQ=255)                                                                                                                   tttCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGCCGCCGGGTCAGCTTTTGATAATAAAAGTATATCCTTCCATTTGACAATTCACAATACGTTATTTTGCGGTTGTTTCAGaaa  >  4:128019/1‑139 (MQ=255)                                                                                                                   tttCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAAGTATATCCTTCCATTTGACAATTCACAATACGTTATTTTGCGGTTGTTTCAGaaa  >  2:164104/1‑139 (MQ=255)                                                                                                                   tttCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAAGTATATCCTTCCATTTGACAATTCACAATACGTTATTTTGCGGTTGTTTCAGaaa  >  4:300666/1‑139 (MQ=255)                                                                                                                    ttCACATCGAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCATTTGATAATAAAAGTATATCCTACCAATTGACAATTCACAATACGGTATTTTGCGGTTGTTTCAGaaa  >  6:216205/1‑138 (MQ=255)                                                                                                                            |                                                                                                                                  CGGTGTCTCCAGTAGCTGCGGCAAGTACCAATAGTATCCGTATACCGTTCCCAGAAAATTGATGGCTAAGACAAGAATAAGCATCGGCTGCCGTCCTAATACATATTGAAACCATTTCACATCAAATCAACTCTCAGTCTGTAAAAATAATATGTCCATATAAAAAAGCTGACGCCGGGTCAGCTTTTGATAATAAAAGTATATCCTTCCATTTTACAATTCACAACACGTTATTTTGCGGTTGTTTCAGAAA  >  CP006881/2180728‑2180980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑