Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,181,189	T→G	T179T (ACA→ACC)	U712_10920 ←	Menaquinol‑cytochrome c reductase cytochrome b/c subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,181,189	0	T	G	100.0%	180.1 / NA	55	T179T (ACA→ACC)	U712_10920	Menaquinol‑cytochrome c reductase cytochrome b/c subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (30/25);  total (30/25)

CAGCAATTTTTTTGATTTCATCCGGCTTCAGCCCGCTGTCAACCAACGAAGGCCCGGCTGCCCCTCCTTGAAGGTTATCTCCATGGCAAGAAATACAGCCCTGCTCCTTAAAAACCTTATATCCTTCTGCATTTGTGTCAATGTCGGCTTCTTTTGTGATTTTTCCCTGTTCTTCTGCCTTGGCCCAGTCGTGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATCGCCAAAAGCATCATACCAACAGCAACAGGA  >  CP006881/2181056‑2181321                                                                                                                                      |                                                                                                                                     cAGCGATTTTTTTGATTTCATCCGGTTTCAGCCCGCTGTCAACCAATGAAGGCCCGGCCGCTCCTCCCTGAAGGTTATCCCCATGGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTggtg                                                                                                                                   <  7:80471/137‑1 (MQ=255)  aGCGATTTTTTTGATTTCATCCGGTTTCAGCCCGCTGTCAACCAATGAAGGCCCGGCCGCTCCTCCCTGAAGGTTATCCCCATGGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTc                                                                                                                                 >  2:347947/1‑138 (MQ=255)    cgaTTTTTTTGATTTCATCCGGTTTCAGCCCGCTGTCAACCAATGAAGGCCCGGCCGCTCCTCCCTGAAGGTTATCCCCATGGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTATTCATTGGTTTCAAt                                                                                                                              >  6:70160/3‑139 (MQ=255)    cgaTTTTTTTGATTTCATCCGGTTTCAGCCCGCTGTCAACCAATGAAGGCCCGGCCGCTCCTCCCTGAAGGTTATCCCCATCGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAAt                                                                                                                              >  4:360115/3‑139 (MQ=255)         tttttGATTTCATCCGGTTTCAGCCCGCTGTCAACCAATGAAGGCCCGGCCGCTCCTCCCTGAAGGTTATCCCCCTGGCAAGAAATACAGCCCTGTTCCTTAATAACCTTATATCCTTCTGCATTGGTGTCAATGTCgg                                                                                                                         >  4:421697/1‑139 (MQ=255)           tttGATTTCATCCGGTTTCAGCCCGCTGTCAACCAATGAAGGCCCGGCCGCTCCTCCCTGAAGGATATCACCATGGCAAGAAATACAGCCCTTTTACTTAAAAACCTTATATCCTACTGCTATGGTGTCAATGTCGGct                                                                                                                       >  6:442284/1‑139 (MQ=255)           tttGATTTCATCCGGTTGCAGCCCGCTGTCAACCAAGGAAGGCCCGGCCGCTCCTCCCTGAAGGTTATCCCCATGGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGc                                                                                                                        <  8:324427/138‑1 (MQ=255)                             cagccagcTGTCAACCAATGAAGGCCCGGCCGCTCCTCCCTGAAGGTTATCCCCATGGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTccc                                                                                                      <  5:442284/138‑1 (MQ=255)                             cAGCCCGCTGTCAACCAATGAAGGCCCGGCCGCTCCTCCCTGAAGGTTATCCCCATGGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTccc                                                                                                      <  4:399970/138‑1 (MQ=255)                             cAGCCCGCTGTCAACCAATGAAGGCCCGGCCGCTCCTCCCTGAAGGTTATCCCCATGGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTccc                                                                                                      <  3:360115/138‑1 (MQ=255)                                    cTGTGAACCAATGAAGGCCCGGCCGCTCCTCCCTGAAGGTTATCCCCATGGCAAGAACTACAGCCCTGTTCCTTAAAAACCTTTTATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGttctt                                                                                              <  1:300250/139‑1 (MQ=255)                                    cTGTCAACCAATGAAGGCCCGGCCGCTCCTCCCTGAAGGTTATCCCCATGGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGttctt                                                                                              <  3:167479/139‑1 (MQ=255)                                            caatGAAGGCCCGGCCGCTCCTCCCTGAAGGTTATCCCCATGGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGTTCTTCTGCCTTg                                                                                      <  3:202458/139‑1 (MQ=255)                                               tgAAGGCCCGGCCGCTCCTCCCTGAAGGTTATCCCCATGGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGTTCTTCTGcc                                                                                         >  8:26477/2‑133 (MQ=255)                                               tgAAGGCCCGGCCGCTCCTCCCTGAAGGTTATCCCCATGGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGTTCTTCTGcc                                                                                         <  7:26477/132‑1 (MQ=255)                                               tgAAGGCCCGGCCGCTCCTCCCTGAAGGTTATCCCCATGGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGTTCTTCTGCCTTGGcc                                                                                   <  1:334295/138‑1 (MQ=255)                                               tgAAGGCCCGGCCGCTCCTCCCTGAAGGTTATCCCCATGGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGTTCTTCTGCCTTGGcc                                                                                   <  1:347947/138‑1 (MQ=255)                                               tgAAGGCCCGGCCGCTCCTCCCTGAAGGTTATCCCCATGGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGTTCTTCTGCCTTGGcc                                                                                   <  5:70160/138‑1 (MQ=255)                                                      ccGGCCGCTCCTCCCTGAAGGTTATCCCCATGGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTGTTTTGTAATTTTACCCTGTTCTTCTGCCTTGGCCCAggcat                                                                            >  1:341614/1‑135 (MQ=255)                                                       cGGCCGCTCCTCCCTGAAGGTTATCCCCATGGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTGCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTGCCCTGTTGTTGTGGCTTGGCCCAGTCATg                                                                           >  7:407767/1‑139 (MQ=255)                                                                  ccctGAAGGTTATCCCCATGGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTccc                                                                                                      >  3:96999/4‑101 (MQ=255)                                                                  ccctGAAGGTTATCCCCATGGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTccc                                                                                                      <  4:96999/98‑1 (MQ=255)                                                                        aGGTTATCCCCATGGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTATGTAATTTTCCCCTGTTCTTCTGCCTTGGCCCAGTCATGAGTAGCAACTGACTGcc                                                          <  7:51091/139‑1 (MQ=255)                                                                             aTCCCCATGGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTTATTTTCCCCGGTTATTCAGCCTTGGCCCCGTCAGGAGTTGCGACTGACTGCCagct                                                      >  1:381493/1‑135 (MQ=255)                                                                             aTCCCCATGGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTcccc                                                                                                     >  2:250504/1‑91 (MQ=255)                                                                             aTCCCCATGGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTcccc                                                                                                     <  1:250504/91‑1 (MQ=255)                                                                             aTCCCCATGGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGTTCTTCTGCCTTGGCCCAGTCATGAGTAGCAACTGACTGCCACGt                                                      >  3:216733/1‑138 (MQ=255)                                                                             aTCCCCATGGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCATCGTTTGTAATTTTCCCCTGTTCTTCTGCCTTGGCCAAGTCAAGAGTACAAAATGACTGCCAcct                                                      >  5:142013/1‑136 (MQ=255)                                                                                   aTGGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTGTTTTGTAATTTTCCCCTGTTATTCTGCCTTGGCCCAGTCATGAGTAGCAACTGACTGCCCCGTCaaaaa                                                >  4:431748/1‑138 (MQ=255)                                                                                   aTGGCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGTTCTTCTGCCTTGGCCCAGTCATGAGTAGCAACTGACTGCCACGTCaaaaa                                                >  2:5271/1‑138 (MQ=255)                                                                                     ggCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAAtt                                                                                                           >  6:94998/1‑77 (MQ=255)                                                                                     ggCAAGAAATACAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAAtt                                                                                                           <  5:94998/77‑1 (MQ=255)                                                                                                cAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTtg                                                                                                                >  4:72252/1‑61 (MQ=255)                                                                                                cAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTtg                                                                                                                <  3:72252/61‑1 (MQ=255)                                                                                                cAGCCCTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGTTCTTCTGCCTTGGCCCAGTCATGAGTATCAACTGACTGCCACGTCAAAAATACAGCAGCGGaaa                                  >  6:179350/1‑139 (MQ=255)                                                                                                    ccTGTTCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGTTCTTCTGCCTTGGCCCAGTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGc                              <  4:159922/139‑1 (MQ=255)                                                                                                      tgttCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTTATTTTCCCCAGTTATTATGGCGTGGCCCAGTAATGAATAGCAACTGACTGACACGTCAAAAAAACAGCACCGGAAATGGc                              >  1:368710/4‑137 (MQ=255)                                                                                                      tgttCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGTTCTTATGCCTTGGCCCACTCATGAGTAGCATCTTACTGCCACCTCAAAAATACAGCAGCCGAAATGGc                              >  4:330205/4‑137 (MQ=255)                                                                                                        ttCCTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGTTCTTCTGCCTTGGCCCAGTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCag                           <  6:318223/137‑2 (MQ=255)                                                                                                           cTTAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGTTCTTCTGCCTTGGCCCAGTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGc                       <  1:444095/139‑1 (MQ=255)                                                                                                             tAAAAACCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCACCTGTTATTCTGCCTTGGCCCAGTCATGAGTAGCAACTGACTGCCACCTCAAAAATACAGCAGCGGAAATTGCCAGAAGca                      >  6:236650/1‑138 (MQ=255)                                                                                                                  aCCTTATATCCTTCTGCATTGGTGTCAATGTCGGCTTCGTTTGTAATTTTCCCCGGTTCTTATGCCTTGGCCCAGGCATGAGTAGCAACAGACTGCCCCCGCAAAAATACCGCCGCGGAAAAGGCCAGAAGCATCATAc                >  4:445888/1‑139 (MQ=255)                                                                                                                     ttATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGTTCTTCTGCCTTGGCCCAGTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCag                           >  6:320030/1‑124 (MQ=255)                                                                                                                     ttATATCCTTCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGTTCTTCTGCCTTGGCCCAGTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCag                           <  5:320030/125‑2 (MQ=255)                                                                                                                             ttCTGCATTGGTGTCAATGTCGGCTTCTTTTGTTATTTTCCCCTGTTCTTCGGCCGTGGCCCCGTCATGAGTAGCAACTGACTGCCCCGGCAAAAATACAGCAGCCGAAATTGCCCGAAGCATCATACcgacagcgccc     >  6:356716/1‑135 (MQ=255)                                                                                                                              tCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGTTCTTCTGCCTTGGCCCAGTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCCGCGGAAATGGCCAGAAGCATCATACcgac            <  8:193321/131‑1 (MQ=255)                                                                                                                              tCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGTTCTTCTGCCTTGGCCCAGTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGCAATGGCCAGAAGCATCCTACcgacagcgcccg    >  2:277214/1‑134 (MQ=255)                                                                                                                              tCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGTTCTTCTGCCTTGGCCCAGTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCag                           >  1:93837/1‑115 (MQ=255)                                                                                                                              tCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGTTCTTCTGCCTTGGCCCAGTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCag                           <  2:93837/116‑2 (MQ=255)                                                                                                                              tCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGTTCTTCTGCCTTGGCCCAGTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACcgacagcgaccg    >  2:53846/1‑137 (MQ=255)                                                                                                                              tCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGTTCTTCTGCCTTGGCCCAGTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACcgac            >  7:193321/1‑131 (MQ=255)                                                                                                                              tCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGTTCTTCTGCCTTGGCCCAGTCATGAGTAGCAACTGACTGCCACGTCAAAAAAACAGCAGCGGAAATGGCCAGAAGCATCATACcgacagcgaccg    >  7:335403/1‑137 (MQ=255)                                                                                                                              tCTGCATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGTTATTCAGCCTTGGCCCAGTCATGAGTAGCAACTTACTGCCACGTCAAAAATACAGCAGCCGAAATGGCCAGAAGCATCATACcgacagcgaccg    >  7:66600/1‑137 (MQ=255)                                                                                                                               cTGCCTTGGGGTCAATGTCGGCTTCTTTTTTAATTTTCCCATGTTCTTCTGCCTGGGCCCAGGCAGGAGTAGCAACTGACTGCCACGTCAAAAAAACCGCAGCGGAAATGGCCAGACGCATCATACcgacagcgaccg    <  1:370465/138‑3 (MQ=255)                                                                                                                                  cATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGTTCTTCTGCCTTGGCCCAGTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGa  <  4:216733/137‑1 (MQ=255)                                                                                                                                  cATTGGTGTCAATGTCGGCTTCTTTTGTAATTTTCCCCTGTTCTTCTGCCTTGGCCCAGTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGa  <  5:179350/137‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     CAGCAATTTTTTTGATTTCATCCGGCTTCAGCCCGCTGTCAACCAACGAAGGCCCGGCTGCCCCTCCTTGAAGGTTATCTCCATGGCAAGAAATACAGCCCTGCTCCTTAAAAACCTTATATCCTTCTGCATTTGTGTCAATGTCGGCTTCTTTTGTGATTTTTCCCTGTTCTTCTGCCTTGGCCCAGTCGTGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATCGCCAAAAGCATCATACCAACAGCAACAGGA  >  CP006881/2181056‑2181321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑