Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,181,339	C→T	E129E (GAG→GAA)	U712_10920 ←	Menaquinol‑cytochrome c reductase cytochrome b/c subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,181,339	0	C	T	100.0%	122.8 / NA	42	E129E (GAG→GAA)	U712_10920	Menaquinol‑cytochrome c reductase cytochrome b/c subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/23);  total (19/23)

GCTTCTTTTGTGATTTTTCCCTGTTCTTCTGCCTTGGCCCAGTCGTGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATCGCCAAAAGCATCATACCAACAGCAACAGGACGCTTCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAA  >  CP006881/2181202‑2181464                                                                                                                                          |                                                                                                                              gCTTCTTTTGTGATTTTTCGCTGTTCTTCTGCCTTGGCCCAGTCGTGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATCGCCAAAAGCATCATCCCAACAGCAACAGGACGCTTCCACGGCCTtctct                                                                                                                              <  1:377092/139‑1 (MQ=255)     cttTTGTAATTTTCCCCTGTTCTTCTGCCTTGGCCCCGTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCttctttct                                                                                                                            <  2:341614/137‑1 (MQ=255)          gtaaTTTTCCCCTGTTCTTCTGCCTTGGCCCAGTGATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCct                                                                                                                      <  6:142013/135‑1 (MQ=255)          gtaaTTTTCCCCTGTTCTTCTGCCTTGGCCCAGTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCct                                                                                                                      <  8:335403/135‑1 (MQ=255)          gtaaTTTTCCCCTGTTCTTCTGCCTTGGCCCAGTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCct                                                                                                                      <  1:5271/135‑1 (MQ=255)          gtaaTTTTCCCCTGTTCTTCTGCCTTGGCCCAGTAATGAGTAGCAACTGACTGCCACGTCAAAAATACACCAGCGGAAATGGCCAGAAGCATCATCCCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCct                                                                                                                      <  5:236650/135‑1 (MQ=255)          gtaaTTTTCCCCTGTGCTTCTGCCTTGGCCCAGTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGCCAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCct                                                                                                                      <  2:381493/135‑1 (MQ=255)          gtaaTTTTCCCCTGATCTCCTCCCTTGGCCCAGTCATGAGTCGCACCTCACTCCCACGTAAAAAAAACCCCCCCGGAAATGGCCATACGATTCATACCGACCGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCct                                                                                                                      <  6:256933/135‑1 (MQ=255)             aTTTTCCCCTGTTCTTCTGGCTTGGCCCAGTCATGAGTAGCAACTGACTGCCACGTCAAAAATACCGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCCTCTg                                                                                                                   <  3:337231/138‑1 (MQ=255)             aTTTTCCCCTGTTCTTCTGCCTTGGCCCAGTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCCTCTg                                                                                                                   <  3:330205/138‑1 (MQ=255)                ttccccTGTTCTTCTGCCTTGGCCCAGTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCct                                                                                                                      <  7:282070/129‑1 (MQ=255)                ttccccTGTTCTTCTGCCTTGGCCCAGTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCct                                                                                                                      >  8:282070/4‑132 (MQ=255)                                  ttGGCCCAGTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCGTGCCCTCGGTCTAGGAATGGAGCCAAAAg                                                                                               >  2:101529/1‑137 (MQ=255)                                  ttGGCCCAGTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCGGTCCCTCTGTCTAGGAATGGCGCCAAAAg                                                                                               >  5:78749/1‑137 (MQ=255)                                    ggCCCAGTCATGAGTAGCAACGTACTGCCACGTCAAAAATACAGCATCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAg                                                                                            <  6:341453/138‑1 (MQ=255)                                         aGTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGcccc                                                                                      <  1:53846/139‑1 (MQ=255)                                          gTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCct                                                                                                                      >  8:47414/1‑106 (MQ=255)                                          gTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCct                                                                                                                      <  7:47414/106‑1 (MQ=255)                                          gTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGcccc                                                                                      >  4:125329/1‑138 (MQ=255)                                          gTCATGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTATGTCCCTCTGTCTAGGAATTGAGCCAAAAGCCGGGcccc                                                                                      >  5:341453/1‑138 (MQ=255)                                                aGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCg                                                                               >  3:188128/1‑139 (MQ=255)                                                     cAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCct                                                                                                                      >  8:103471/1‑95 (MQ=255)                                                     cAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCct                                                                                                                      <  7:103471/95‑1 (MQ=255)                                                     cAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTGTAGGAATGGCGCCCAAAGCACGGCCCCAAAAGCgcgacc                                                                          >  3:448112/1‑134 (MQ=255)                                                     cAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGAcc                                                                          >  3:402801/1‑139 (MQ=255)                                                             tgCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCGTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGCCCGGGCCTaa                                                                  >  5:116052/1‑139 (MQ=255)                                                               ccACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTGAGTCCCGCAGTCTAGGAATGGAGCCAAAAGCAGGGGCCCAAAAGCGAGCCCTGGCATAAt                                                                 >  3:151480/1‑138 (MQ=255)                                                                cACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCAAGGAATGGAGCCAAAAGCAGGGCCCCAAAAACGACACCAGGCaaaaaaa                                                               >  2:63968/1‑136 (MQ=255)                                                                cACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCCGCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGCCCGGTCAAAATCa                                                               >  8:105743/1‑139 (MQ=255)                                                                       aaaaTACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGCCCGGCCGCTTCCACGGCCTTCTTTCTGTCCCGCTGTCGTGGGATTGAGGCACAAGCAGGGCCCCACAAGCGCGACCAGGCATAATAATGGCGcc                                                        >  8:40223/1‑139 (MQ=255)                                                                          aTACAGCCGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGAc                                                     <  1:63968/139‑1 (MQ=255)                                                                          aTACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGAc                                                     <  7:105743/139‑1 (MQ=255)                                                                          aTACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGAATTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAATCAGGGCCCCAAAAGCGATACCTGGCATAATCATGGCGCCGAc                                                     <  8:66600/139‑1 (MQ=255)                                                                                 agcGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTa                                              <  3:431748/139‑1 (MQ=255)                                                                                     gAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTTTGCCACTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAACCAGACCTGGCATAATAATGGCGCCGCCCCCTGTaaacc                                          >  2:108882/1‑137 (MQ=255)                                                                                              agaaGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCcggcgg                                 <  4:236374/137‑1 (MQ=255)                                                                                               gaaccatcatACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCggcggc                                <  4:151480/135‑1 (MQ=255)                                                                                                             acagcgaccgGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAgac                                                                           >  2:396970/1‑80 (MQ=255)                                                                                                             acagcgaccgGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAgac                                                                           <  1:396970/82‑3 (MQ=255)                                                                                                             acagcgaccgGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTc                   >  7:59849/1‑138 (MQ=255)                                                                                                             acagcgaccgGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCa                  <  4:402801/139‑1 (MQ=255)                                                                                                                   accggACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAg              >  7:44192/4‑137 (MQ=255)                                                                                                                    ccggACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGc             <  8:44192/135‑1 (MQ=255)                                                                                                                              ccACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATaagaa  <  5:356716/138‑1 (MQ=255)                                                                                                                                          |                                                                                                                              GCTTCTTTTGTGATTTTTCCCTGTTCTTCTGCCTTGGCCCAGTCGTGAGTAGCAACTGACTGCCACGTCAAAAATACAGCAGCGGAAATCGCCAAAAGCATCATACCAACAGCAACAGGACGCTTCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAA  >  CP006881/2181202‑2181464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑