Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,181,393 C→T P111P (CCG→CCA)  U712_10920 ← Menaquinol‑cytochrome c reductase cytochrome b/c subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,181,3930CT96.0% 146.9 / NA 52P111P (CCG→CCA) U712_10920Menaquinol‑cytochrome c reductase cytochrome b/c subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (21/27);  minor base G (2/0);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 2.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCCACGTCAAAAATACAGCAGCGGAAATCGCCAAAAGCATCATACCAACAGCAACAGGACGCTTCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCTGTATCAGTCGGATCCGCCATC  >  CP006881/2181262‑2181519
                                                                                                                                   |                                                                                                                              
tgCCACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCGTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGCCCGGGCCTaa                                                                                                                         >  5:116052/1‑139 (MQ=255)
  ccACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTGAGTCCCGCAGTCTAGGAATGGAGCCAAAAGCAGGGGCCCAAAAGCGAGCCCTGGCATAAt                                                                                                                        >  3:151480/1‑138 (MQ=255)
   cACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCAAGGAATGGAGCCAAAAGCAGGGCCCCAAAAACGACACCAGGCaaaaaaa                                                                                                                      >  2:63968/1‑136 (MQ=255)
   cACGTCAAAAATACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCCGCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGCCCGGTCAAAATCa                                                                                                                      >  8:105743/1‑139 (MQ=255)
          aaaaTACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGCCCGGCCGCTTCCACGGCCTTCTTTCTGTCCCGCTGTCGTGGGATTGAGGCACAAGCAGGGCCCCACAAGCGCGACCAGGCATAATAATGGCGcc                                                                                                               >  8:40223/1‑139 (MQ=255)
             aTACAGCCGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGAc                                                                                                            <  1:63968/139‑1 (MQ=255)
             aTACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGAc                                                                                                            <  7:105743/139‑1 (MQ=255)
             aTACAGCAGCGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGAATTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAATCAGGGCCCCAAAAGCGATACCTGGCATAATCATGGCGCCGAc                                                                                                            <  8:66600/139‑1 (MQ=255)
                    agcGGAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTa                                                                                                     <  3:431748/139‑1 (MQ=255)
                        gAAATGGCCAGAAGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTTTGCCACTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAACCAGACCTGGCATAATAATGGCGCCGCCCCCTGTaaacc                                                                                                 >  2:108882/1‑137 (MQ=255)
                                 agaaGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCcggcgg                                                                                        <  4:236374/137‑1 (MQ=255)
                                  gaaccatcatACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCggcggc                                                                                       <  4:151480/135‑1 (MQ=255)
                                                acagcgaccgGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTc                                                                          >  7:59849/1‑138 (MQ=255)
                                                acagcgaccgGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCa                                                                         <  4:402801/139‑1 (MQ=255)
                                                      accggACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAg                                                                     >  7:44192/4‑137 (MQ=255)
                                                       ccggACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGc                                                                    <  8:44192/135‑1 (MQ=255)
                                                                 ccACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATaagaa                                                         <  5:356716/138‑1 (MQ=255)
                                                                                 gtcccTCTGTCTAGGAATGGAGCCAAATGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACa                                                                      <  3:49500/106‑1 (MQ=255)
                                                                                 gtcccTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACa                                                                      <  5:126411/106‑1 (MQ=255)
                                                                                 gtcccTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACa                                                                      >  4:49500/4‑109 (MQ=255)
                                                                                 gtcccTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGACGACCACTGTAAAGCTTCCGGCGGCGAATTCATACTTCAACAGCTCGTATAACAAAAGAAAATACCAATCCg                                        >  3:173210/4‑139 (MQ=255)
                                                                                 gtcccTCTGTCTAGGAATGCAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACa                                                                      >  6:126411/4‑109 (MQ=255)
                                                                                     ctctGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAg                                    <  1:108882/139‑1 (MQ=255)
                                                                                     ctctGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAg                                    <  7:40223/139‑1 (MQ=255)
                                                                                     ctctGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAg                                    <  3:125329/139‑1 (MQ=255)
                                                                                     ctctGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAg                                    <  3:445888/139‑1 (MQ=255)
                                                                                     ctctGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACATAAAAAGCTGGTATAACTAAAGAAAAAACCAAACCGGcct                                    >  1:82807/1‑137 (MQ=255)
                                                                                      tctGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAg                                    >  8:265434/1‑138 (MQ=255)
                                                                                      tctGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAg                                    <  7:265434/138‑1 (MQ=255)
                                                                                      tctGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGc                                   >  5:223178/1‑139 (MQ=255)
                                                                                           ctaGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAat                              <  4:448112/137‑1 (MQ=255)
                                                                                              ggAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAata                             <  6:78749/137‑1 (MQ=255)
                                                                                               gAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTAATAAATAAACAGCAGGGATAAGAAAAGAAAATAACAAAACGGCAACGGCAtata                           >  6:234985/1‑138 (MQ=255)
                                                                                                aaTGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAAc                         <  1:101529/139‑1 (MQ=255)
                                                                                                  tGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAAtata                           <  2:396374/135‑1 (MQ=255)
                                                                                                  tGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAAtata                           >  1:396374/1‑135 (MQ=255)
                                                                                                  tGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAAc                         >  4:120155/1‑137 (MQ=255)
                                                                                                    gagcCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGCCCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGGATAAGAAAAGAAAATACCAATCCGGCCGCGCAATATAAccag                      >  7:444184/3‑136 (MQ=255)
                                                                                                    gagcCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCggcggc                                                                                       >  3:107657/3‑73 (MQ=255)
                                                                                                    gagcCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCggcggc                                                                                       <  4:107657/71‑1 (MQ=255)
                                                                                                    gagcCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACCGCGGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAAccag                      >  4:293912/3‑136 (MQ=255)
                                                                                                    gagcCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGc                                                                    <  2:256968/90‑1 (MQ=255)
                                                                                                    gagcCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGc                                                                    >  1:256968/3‑92 (MQ=255)
                                                                                                    gagcCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAAccag                      >  6:72474/3‑136 (MQ=255)
                                                                                                    gagcCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAAccag                      >  1:320250/3‑136 (MQ=255)
                                                                                                          aaaGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGt               <  4:188128/139‑1 (MQ=255)
                                                                                                                 ggCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCCATCCGGCAGCGGAATATACCCAGTATCAGTCGGGTcc        >  4:258531/1‑139 (MQ=255)
                                                                                                                 ggCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTAATACTTAAACAGCTGGTATAAGAAAAGAAAATACCAATCCAGCACCGGAAAATAACCAGTAACAGGCGGGTcc        >  6:258858/1‑139 (MQ=255)
                                                                                                                       aaaaGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATc  <  7:292661/139‑1 (MQ=255)
                                                                                                                       aaaaGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATc  <  4:20341/139‑1 (MQ=255)
                                                                                                                       aaaaGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATc  <  6:223178/139‑1 (MQ=255)
                                                                                                                       aaaaGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATc  <  8:59849/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                              
TGCCACGTCAAAAATACAGCAGCGGAAATCGCCAAAAGCATCATACCAACAGCAACAGGACGCTTCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCTGTATCAGTCGGATCCGCCATC  >  CP006881/2181262‑2181519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑