Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,181,555 A→G L57L (CTT→CTC)  U712_10920 ← Menaquinol‑cytochrome c reductase cytochrome b/c subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,181,5550AG93.5% 260.4 / ‑4.6 92L57L (CTT→CTC) U712_10920Menaquinol‑cytochrome c reductase cytochrome b/c subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (53/33);  minor base C (3/3);  total (56/36)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCTGTATCAGTCGGATCCGCCATCCGCTCCAAAGGCGGCTGATGCACAATCGTCAAAACAAGAAAACCGATCAGAAAGACAGCTCCAACCATCCATTCCTTCAACAAGAAATTCGGCCAGAAAGCCTCTGTTTTACCGGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGCCGGTATCCT  >  CP006881/2181422‑2181686
                                                                                                                                     |                                                                                                                                   
aGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGaa                                                                                                                                 >  5:261814/1‑138 (MQ=255)
  cTTCCGGCGGCGTATTGATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAAc                                                                                                                              >  4:368174/1‑139 (MQ=255)
     ccGGCGGCGTTTTGATACATGCACAGCTGGGTTAAGATAACAAAATACCAATCCGGCAGGGGAATTTAACGCGTATCAGTGCGGTCCGCCATCCGGTCGTAAGGCGGGTGATGCGCCATCGTCCAAACCAGAAAAcc                                                                                                                             >  4:279026/1‑137 (MQ=255)
     ccGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCg                                                                                                                            <  5:234985/138‑1 (MQ=255)
     ccGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGa                                                                                                                           >  1:239785/1‑139 (MQ=255)
     ccGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGGATCAGTCGGGTCCGCCATCCGCTCTACAGGCGGCTGATGCACAATCGTCAAAACGAGAAAAcc                                                                                                                             >  4:323948/1‑137 (MQ=255)
     ccGGCGGCGTATTAATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATACGCACAAAAGGCGGCTGCAGCACAAAAGTCAAAACCCGAAAACCgc                                                                                                                           >  1:156006/1‑138 (MQ=255)
     ccGGCGGCGTATTAATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAAAACGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGCTGCACAATCGTCAAAACCAGAAAACCg                                                                                                                            >  6:266005/1‑138 (MQ=255)
            cgTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATTACCAGTATCAGTCGGGTCCGCCCTCCGCTCTAAAGGCGGCGGATGCACAATCGTCAAAACGAGAAACCCCATgaga                                                                                                                      >  8:11878/1‑137 (MQ=255)
              tATTCATACTTCAACAGCTGGTATAAGAAAAGAAAAAACCAATCCGGCCGCGGAATAAACCCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGa                                                                                                                  <  3:258531/139‑1 (MQ=255)
                  cATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAg                                                                                                               <  2:320250/138‑1 (MQ=255)
                  aaTACTTCAACAGCTGGTATAATAAAAGAAAATACCAATCCAGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGTTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAg                                                                                                               <  5:258858/137‑1 (MQ=255)
                        tCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAAc                                                                                                        >  7:343238/1‑139 (MQ=255)
                         cAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCaa                                                                                                         <  4:365888/137‑1 (MQ=255)
                         cAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAAcc                                                                                                       >  1:353104/1‑139 (MQ=255)
                         cAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAAcc                                                                                                       >  8:133232/1‑139 (MQ=255)
                         cAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAAc                                                                                                        >  5:287703/1‑138 (MQ=255)
                               cTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGCTGCACAATCGTCCAAACGAGAAAACCGATGAGAAAGACAGCTCCAAacatccat                                                                                                 >  8:53542/1‑139 (MQ=255)
                               cTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGCTGAGAAAGACAGCTCCCAccctgtct                                                                                                 >  3:244916/1‑133 (MQ=255)
                                 ggTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAACACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTc                                                                                               >  3:26066/1‑139 (MQ=255)
                                 ggTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTc                                                                                               >  2:92714/1‑139 (MQ=255)
                                 ggTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTc                                                                                               >  5:426233/1‑139 (MQ=255)
                                 ggTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTc                                                                                               >  6:361200/1‑139 (MQ=255)
                                 ggTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTc                                                                                               >  7:118832/1‑139 (MQ=255)
                                          aaagaaaaTACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTtaacaa                                                                                      <  3:368174/139‑1 (MQ=255)
                                            agaaaaTACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCCTTCCTTTAACAAg                                                                                     >  4:243134/1‑138 (MQ=255)
                                                      aaTCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGc                                                                            >  6:126812/1‑137 (MQ=255)
                                                       aTCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGc                                                                            >  6:135768/1‑136 (MQ=255)
                                                        tCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAg                                                                         >  3:258371/1‑138 (MQ=255)
                                                        tCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAg                                                                         >  7:245216/1‑138 (MQ=255)
                                                            gCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACCGCTCCACCCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGc                                                                    >  5:367771/1‑139 (MQ=255)
                                                            gCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGc                                                                    >  6:107008/1‑139 (MQ=255)
                                                             cAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGcc                                                                   >  8:135068/1‑139 (MQ=255)
                                                              agcttaaTATAACCAGTATCAGTCGGGTCCGCGATCCGCTCTATAGGCGGCTGATGCAAATTCGTCAAAACGATAAAACCTATGAGAAAGACAGCTCCAACCATCCATTCCTTTAAGAAGAAATTTGGCCAGAAAGCct                                                                  <  7:263170/134‑1 (MQ=255)
                                                              aGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCct                                                                  <  5:107008/139‑1 (MQ=255)
                                                              aGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCct                                                                  <  2:239785/139‑1 (MQ=255)
                                                                  taaTATAACCAGTATCTGGCGGGTCCGCCATCCGCTCTAACGGCGGGTGATGCACAATCGTCAAAAGGCGAAAACCTAGGACAATGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGt                                                              <  8:343238/138‑1 (MQ=255)
                                                                    ataaAACCTGTATCAGTCGGGCCCGCAAGCCGCTCTAAAGCCGGGTTAGTAACAATCGACACAACCATAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGttt                                                            <  2:82807/139‑1 (MQ=255)
                                                                       tacccagTAGCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTa                                                          <  5:72474/132‑1 (MQ=255)
                                                                       taACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAACCGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTa                                                          <  2:252692/138‑1 (MQ=255)
                                                                       taACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCct                                                                  <  4:213344/130‑1 (MQ=255)
                                                                       taACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCct                                                                  >  3:213344/1‑130 (MQ=255)
                                                                             gTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAg                                                                                                                       >  7:29450/1‑71 (MQ=255)
                                                                             gTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAg                                                                                                                       <  8:29450/71‑1 (MQ=255)
                                                                             gTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGa                                                    >  1:169226/1‑138 (MQ=255)
                                                                             gTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGa                                                    >  6:163380/1‑138 (MQ=255)
                                                                             gTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGa                                                    >  6:186655/1‑138 (MQ=255)
                                                                             gTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGa                                                    >  7:166799/1‑138 (MQ=255)
                                                                             gTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGa                                                    >  3:199983/1‑138 (MQ=255)
                                                                             gTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGa                                                    >  2:59463/1‑138 (MQ=255)
                                                                             gTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTAAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTAcccctgg                                                   >  1:39099/1‑134 (MQ=255)
                                                                                tccgTCGGGTCCGCCATCCTCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACt                                                <  8:329010/136‑1 (MQ=255)
                                                                                tCAGTCGGGTCCGCCATCCGCTCTAACGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACt                                                <  3:120155/139‑1 (MQ=255)
                                                                                tCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACt                                                <  8:118832/139‑1 (MQ=255)
                                                                                  aGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCATTAACAAGAAATTTGGCCAGAAAGCCTCTGATTTACCCGGATACTcc                                              >  1:21828/1‑139 (MQ=255)
                                                                                   gTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTcc                                              >  1:353544/1‑138 (MQ=255)
                                                                                    tCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAg                                                                                                                       <  5:318987/64‑1 (MQ=255)
                                                                                    tCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAg                                                                                                                       >  6:318987/1‑64 (MQ=255)
                                                                                    tCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCg                                             >  1:357410/1‑138 (MQ=255)
                                                                                     cGGGTCCGCCATCCGCTCTAATGGCGGCTTCTGCACAATCGTCAAAACGAGAAAACCTATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGaa                                           <  6:287703/139‑1 (MQ=255)
                                                                                      gggtCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGaat                                          <  5:186655/136‑1 (MQ=255)
                                                                                       ggtCCGCCATCCGCTCTAATGGCGGATGATGCACAATCGTAAAAACCAGAAAACCGATCAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGaata                                         <  5:361200/137‑1 (MQ=255)
                                                                                       ggtCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGTCCAGAAAGCCTCTGTTTTACCCGGATACTCCGaat                                          >  3:448445/3‑138 (MQ=255)
                                                                                       ggtCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGaata                                         <  5:163380/137‑1 (MQ=255)
                                                                                       ggtCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGaata                                         <  2:357410/137‑1 (MQ=255)
                                                                                        gtCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGaataa                                        >  3:16970/2‑139 (MQ=255)
                                                                                        gtCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGaataa                                        >  7:145223/2‑139 (MQ=255)
                                                                                        gtCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGaata                                         >  1:358779/2‑138 (MQ=255)
                                                                                           cGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATcc                                     <  4:258371/139‑1 (MQ=255)
                                                                                                  cGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGta                              >  4:237986/1‑139 (MQ=255)
                                                                                                           ggcggcTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCt                     <  4:199983/139‑1 (MQ=255)
                                                                                                              ggcTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACACGGATACTCCGAATAATCCTTCGGTATATTCGGCtttt                  >  6:111881/1‑139 (MQ=255)
                                                                                                               gcTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCttttt                 >  3:447879/1‑139 (MQ=255)
                                                                                                               gcTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCttttt                 >  7:299947/1‑139 (MQ=255)
                                                                                                                   aTGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTc              <  2:353544/138‑1 (MQ=255)
                                                                                                                     gCACAATCGTCAAAACCAGAAAACCGATGAGAAAGACAGCTCCAACCATGCATTCCTTTAACAAGAAATTTGGCCAGATAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGc           <  8:444184/139‑1 (MQ=255)
                                                                                                                      cacaATCGTCAAAACGAGAAAACCGATGAGTAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGc           <  6:261814/138‑1 (MQ=255)
                                                                                                                      cacaATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGc           <  8:245216/138‑1 (MQ=255)
                                                                                                                      cacaATCGTCAAAACGACAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGc           <  5:266005/138‑1 (MQ=255)
                                                                                                                       acaATCGTCAAAACGAGAAAACCGAGGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGCCg         <  5:135768/139‑1 (MQ=255)
                                                                                                                        caATCGTCAAAACGACAAAACCGATGCGAATGACAGCTCCAACCATCCATTCCTTTATCAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGCCgg        <  4:173210/139‑1 (MQ=255)
                                                                                                                         aaTCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGTCTTTTTTTCAGCCgg        >  6:443893/1‑138 (MQ=255)
                                                                                                                          aTCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGaa                                                                       <  1:386879/74‑1 (MQ=255)
                                                                                                                          aTCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGaa                                                                       >  2:386879/1‑74 (MQ=255)
                                                                                                                          aTCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGCCGGTa      >  6:175059/1‑139 (MQ=255)
                                                                                                                           tCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGCCGGTAt     >  3:446561/1‑139 (MQ=255)
                                                                                                                           tCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCATCTGTTTTACACGGATACTCAGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGCCTGTAt     >  3:439696/1‑139 (MQ=255)
                                                                                                                              tCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTc              >  3:225042/1‑127 (MQ=255)
                                                                                                                              tCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTc              <  4:225042/127‑1 (MQ=255)
                                                                                                                              tCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGc           >  3:220204/1‑130 (MQ=255)
                                                                                                                              tCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGc           <  4:220204/130‑1 (MQ=255)
                                                                                                                              tCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGAATATTCGGCTTTTTTTCAGCCGGTATCCt  >  1:81430/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                   
AGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCTGTATCAGTCGGATCCGCCATCCGCTCCAAAGGCGGCTGATGCACAATCGTCAAAACAAGAAAACCGATCAGAAAGACAGCTCCAACCATCCATTCCTTCAACAAGAAATTCGGCCAGAAAGCCTCTGTTTTACCGGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGCCGGTATCCT  >  CP006881/2181422‑2181686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑