Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,181,567 C→G L53L (CTG→CTC)  U712_10920 ← Menaquinol‑cytochrome c reductase cytochrome b/c subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,181,5670CG98.9% 264.9 / NA 87L53L (CTG→CTC) U712_10920Menaquinol‑cytochrome c reductase cytochrome b/c subunit
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (48/38);  total (48/39)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCTGTATCAGTCGGATCCGCCATCCGCTCCAAAGGCGGCTGATGCACAATCGTCAAAACAAGAAAACCGATCAGAAAGACAGCTCCAACCATCCATTCCTTCAACAAGAAATTCGGCCAGAAAGCCTCTGTTTTACCGGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGCCGGTATCCTTGAGTCGCCTACA  >  CP006881/2181434‑2181699
                                                                                                                                     |                                                                                                                                    
cgTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATTACCAGTATCAGTCGGGTCCGCCCTCCGCTCTAAAGGCGGCGGATGCACAATCGTCAAAACGAGAAACCCCATgaga                                                                                                                                   >  8:11878/1‑137 (MQ=255)
  tATTCATACTTCAACAGCTGGTATAAGAAAAGAAAAAACCAATCCGGCCGCGGAATAAACCCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGa                                                                                                                               <  3:258531/139‑1 (MQ=255)
      cATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAg                                                                                                                            <  2:320250/138‑1 (MQ=255)
      aaTACTTCAACAGCTGGTATAATAAAAGAAAATACCAATCCAGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGTTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAg                                                                                                                            <  5:258858/137‑1 (MQ=255)
            tCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAAc                                                                                                                     >  7:343238/1‑139 (MQ=255)
             cAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCaa                                                                                                                      <  4:365888/137‑1 (MQ=255)
             cAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAAcc                                                                                                                    >  1:353104/1‑139 (MQ=255)
             cAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAAcc                                                                                                                    >  8:133232/1‑139 (MQ=255)
             cAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAAc                                                                                                                     >  5:287703/1‑138 (MQ=255)
                   cTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGCTGCACAATCGTCCAAACGAGAAAACCGATGAGAAAGACAGCTCCAAacatccat                                                                                                              >  8:53542/1‑139 (MQ=255)
                   cTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGCTGAGAAAGACAGCTCCCAccctgtct                                                                                                              >  3:244916/1‑133 (MQ=255)
                     ggTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAACACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTc                                                                                                            >  3:26066/1‑139 (MQ=255)
                     ggTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTc                                                                                                            >  7:118832/1‑139 (MQ=255)
                     ggTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTc                                                                                                            >  5:426233/1‑139 (MQ=255)
                     ggTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTc                                                                                                            >  2:92714/1‑139 (MQ=255)
                     ggTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTc                                                                                                            >  6:361200/1‑139 (MQ=255)
                              aaagaaaaTACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTtaacaa                                                                                                   <  3:368174/139‑1 (MQ=255)
                                agaaaaTACCAATCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCCTTCCTTTAACAAg                                                                                                  >  4:243134/1‑138 (MQ=255)
                                          aaTCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGc                                                                                         >  6:126812/1‑137 (MQ=255)
                                           aTCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGc                                                                                         >  6:135768/1‑136 (MQ=255)
                                            tCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAg                                                                                      >  7:245216/1‑138 (MQ=255)
                                            tCCGGCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAg                                                                                      >  3:258371/1‑138 (MQ=255)
                                                gCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACCGCTCCACCCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGc                                                                                 >  5:367771/1‑139 (MQ=255)
                                                gCAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGc                                                                                 >  6:107008/1‑139 (MQ=255)
                                                 cAGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGcc                                                                                >  8:135068/1‑139 (MQ=255)
                                                  agcttaaTATAACCAGTATCAGTCGGGTCCGCGATCCGCTCTATAGGCGGCTGATGCAAATTCGTCAAAACGATAAAACCTATGAGAAAGACAGCTCCAACCATCCATTCCTTTAAGAAGAAATTTGGCCAGAAAGCct                                                                               <  7:263170/134‑1 (MQ=255)
                                                  aGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCct                                                                               <  5:107008/139‑1 (MQ=255)
                                                  aGCGGAATATAACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCct                                                                               <  2:239785/139‑1 (MQ=255)
                                                      taaTATAACCAGTATCTGGCGGGTCCGCCATCCGCTCTAACGGCGGGTGATGCACAATCGTCAAAAGGCGAAAACCTAGGACAATGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGt                                                                           <  8:343238/138‑1 (MQ=255)
                                                        ataaAACCTGTATCAGTCGGGCCCGCAAGCCGCTCTAAAGCCGGGTTAGTAACAATCGACACAACCATAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGttt                                                                         <  2:82807/139‑1 (MQ=255)
                                                           tacccagTAGCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTa                                                                       <  5:72474/132‑1 (MQ=255)
                                                           taACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAACCGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTa                                                                       <  2:252692/138‑1 (MQ=255)
                                                           taACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCct                                                                               >  3:213344/1‑130 (MQ=255)
                                                           taACCAGTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCct                                                                               <  4:213344/130‑1 (MQ=255)
                                                                 gTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAg                                                                                                                                    >  7:29450/1‑71 (MQ=255)
                                                                 gTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAg                                                                                                                                    <  8:29450/71‑1 (MQ=255)
                                                                 gTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGa                                                                 >  6:186655/1‑138 (MQ=255)
                                                                 gTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGa                                                                 >  7:166799/1‑138 (MQ=255)
                                                                 gTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGa                                                                 >  6:163380/1‑138 (MQ=255)
                                                                 gTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGa                                                                 >  2:59463/1‑138 (MQ=255)
                                                                 gTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGa                                                                 >  3:199983/1‑138 (MQ=255)
                                                                 gTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGa                                                                 >  1:169226/1‑138 (MQ=255)
                                                                 gTATCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTAAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTAcccctgg                                                                >  1:39099/1‑134 (MQ=255)
                                                                    tccgTCGGGTCCGCCATCCTCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACt                                                             <  8:329010/136‑1 (MQ=255)
                                                                    tCAGTCGGGTCCGCCATCCGCTCTAACGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACt                                                             <  3:120155/139‑1 (MQ=255)
                                                                    tCAGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACt                                                             <  8:118832/139‑1 (MQ=255)
                                                                      aGTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCATTAACAAGAAATTTGGCCAGAAAGCCTCTGATTTACCCGGATACTcc                                                           >  1:21828/1‑139 (MQ=255)
                                                                       gTCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTcc                                                           >  1:353544/1‑138 (MQ=255)
                                                                        tCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAg                                                                                                                                    >  6:318987/1‑64 (MQ=255)
                                                                        tCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAg                                                                                                                                    <  5:318987/64‑1 (MQ=255)
                                                                        tCGGGTCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCg                                                          >  1:357410/1‑138 (MQ=255)
                                                                         cGGGTCCGCCATCCGCTCTAATGGCGGCTTCTGCACAATCGTCAAAACGAGAAAACCTATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGaa                                                        <  6:287703/139‑1 (MQ=255)
                                                                          gggtCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGaat                                                       <  5:186655/136‑1 (MQ=255)
                                                                           ggtCCGCCATCCGCTCTAATGGCGGATGATGCACAATCGTAAAAACCAGAAAACCGATCAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGaata                                                      <  5:361200/137‑1 (MQ=255)
                                                                           ggtCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGTCCAGAAAGCCTCTGTTTTACCCGGATACTCCGaat                                                       >  3:448445/3‑138 (MQ=255)
                                                                           ggtCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGaata                                                      <  5:163380/137‑1 (MQ=255)
                                                                           ggtCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGaata                                                      <  2:357410/137‑1 (MQ=255)
                                                                            gtCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGaataa                                                     >  3:16970/2‑139 (MQ=255)
                                                                            gtCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGaataa                                                     >  7:145223/2‑139 (MQ=255)
                                                                            gtCCGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGaata                                                      >  1:358779/2‑138 (MQ=255)
                                                                               cGCCATCCGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATcc                                                  <  4:258371/139‑1 (MQ=255)
                                                                                      cGCTCTAAAGGCGGCTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGta                                           >  4:237986/1‑139 (MQ=255)
                                                                                               ggcggcTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCt                                  <  4:199983/139‑1 (MQ=255)
                                                                                                  ggcTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACACGGATACTCCGAATAATCCTTCGGTATATTCGGCtttt                               >  6:111881/1‑139 (MQ=255)
                                                                                                   gcTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCttttt                              >  7:299947/1‑139 (MQ=255)
                                                                                                   gcTGATGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCttttt                              >  3:447879/1‑139 (MQ=255)
                                                                                                       aTGCACAATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTc                           <  2:353544/138‑1 (MQ=255)
                                                                                                         gCACAATCGTCAAAACCAGAAAACCGATGAGAAAGACAGCTCCAACCATGCATTCCTTTAACAAGAAATTTGGCCAGATAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGc                        <  8:444184/139‑1 (MQ=255)
                                                                                                          cacaATCGTCAAAACGAGAAAACCGATGAGTAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGc                        <  6:261814/138‑1 (MQ=255)
                                                                                                          cacaATCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGc                        <  8:245216/138‑1 (MQ=255)
                                                                                                          cacaATCGTCAAAACGACAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGc                        <  5:266005/138‑1 (MQ=255)
                                                                                                           acaATCGTCAAAACGAGAAAACCGAGGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGCCg                      <  5:135768/139‑1 (MQ=255)
                                                                                                            caATCGTCAAAACGACAAAACCGATGCGAATGACAGCTCCAACCATCCATTCCTTTATCAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGCCgg                     <  4:173210/139‑1 (MQ=255)
                                                                                                             aaTCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGTCTTTTTTTCAGCCgg                     >  6:443893/1‑138 (MQ=255)
                                                                                                              aTCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGaa                                                                                    <  1:386879/74‑1 (MQ=255)
                                                                                                              aTCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGaa                                                                                    >  2:386879/1‑74 (MQ=255)
                                                                                                              aTCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGCCGGTa                   >  6:175059/1‑139 (MQ=255)
                                                                                                               tCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGCCGGTAt                  >  3:446561/1‑139 (MQ=255)
                                                                                                               tCGTCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCATCTGTTTTACACGGATACTCAGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGCCTGTAt                  >  3:439696/1‑139 (MQ=255)
                                                                                                                  tCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTc                           >  3:225042/1‑127 (MQ=255)
                                                                                                                  tCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTc                           <  4:225042/127‑1 (MQ=255)
                                                                                                                  tCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGc                        <  4:220204/130‑1 (MQ=255)
                                                                                                                  tCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGc                        >  3:220204/1‑130 (MQ=255)
                                                                                                                  tCAAAACGAGAAAACCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGAATATTCGGCTTTTTTTCAGCCGGTATCCt               >  1:81430/1‑139 (MQ=255)
                                                                                                                               aCCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGCCGGTATCCTTGAGTCGCCTACa  <  5:175059/139‑1 (MQ=255)
                                                                                                                               aCCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGCCGGTATCCTTGAGTCGCCTACa  <  4:447879/139‑1 (MQ=255)
                                                                                                                               aCCGATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGCCGGTATCCTTGAGTCGCCTACa  <  3:293912/139‑1 (MQ=255)
                                                                                                                               aCCCATGAGAAAGACAGCTCCAACCATCCATTCCTTTAACAAGAAATTTGGCCAGAAAGCCTCTGTTTTACCCGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGCCGGTATCCTTGAGTCGCCTACa  <  7:133232/139‑1 (MQ=255)
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CGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCTGTATCAGTCGGATCCGCCATCCGCTCCAAAGGCGGCTGATGCACAATCGTCAAAACAAGAAAACCGATCAGAAAGACAGCTCCAACCATCCATTCCTTCAACAAGAAATTCGGCCAGAAAGCCTCTGTTTTACCGGGATACTCCGAATAATCCTTCGGTATATTCGGCTTTTTTTCAGCCGGTATCCTTGAGTCGCCTACA  >  CP006881/2181434‑2181699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑