Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,172,511	G→C	L5L (CTC→CTG)	U712_10860 ←	3‑methyl‑2‑oxobutanoate hydroxymethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,172,511	0	G	C	100.0%	268.5 / NA	83	L5L (CTC→CTG)	U712_10860	3‑methyl‑2‑oxobutanoate hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (37/46);  total (37/46)

AGGACGACCATTCCGAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGCTTAGCTGCCGGATAATCATAAGCGGTCAGCATCACAATCGGTTCTTCATTCTCCTTCATTTTTAGAAAATCGAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGAGCTGCCGTTTTCTTATACAAGCCAAAAAACCTTCC‑GTT‑TAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCT  >  CP006881/2172377‑2172642                                                                                                                                       |                                                                                                                                      aGGACGCCCATTCCAAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCTGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAgtt                                                                                                                                   >  1:34129/1‑139 (MQ=255) aGGACGACCATTCCAAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAgtt                                                                                                                                   <  7:114888/139‑1 (MQ=255) aGGACGACCATTCCAAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAgtt                                                                                                                                   <  3:442632/139‑1 (MQ=255) aGGACGACCATTCCAAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAgtt                                                                                                                                   <  1:407310/139‑1 (MQ=255)       acCATTCCAAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAgttttgttt                                                                                                                             >  1:243406/1‑139 (MQ=255)         cATTGCAAGTGAATCAGCGACTAAAATGATGTCATCTCCCGCTTGTTCAGCAAGTTTTGCTGCCGGATAATCATAAGAGGTCAGCATGTCAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTc                                                                                                                           <  3:179200/139‑1 (MQ=255)         cATTCCAAGTGACTCACCGACTAAACTCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTc                                                                                                                           <  6:181797/139‑1 (MQ=255)           ttCCAAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATTATCATAATCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCAt                                                                                                                         <  3:348644/139‑1 (MQ=255)           ttCCAAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCAt                                                                                                                         >  8:272513/1‑139 (MQ=255)           ttCCAAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCAt                                                                                                                         >  6:404802/1‑139 (MQ=255)                aGTGAATCACCGACTAAAATCATTTCAACTCCCGCTTGTTCAGCAAGTTTAGCTTCCGGAGAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATAATTAGAAAATCCAGTTTTTTTTACATgctg                                                                                                                     >  4:72592/1‑135 (MQ=255)                aGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGtttt                                                                                                                    >  2:288103/1‑139 (MQ=255)                 gTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTtc                                                                                                                   <  6:98821/139‑1 (MQ=255)                 gTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTtc                                                                                                                   <  6:384989/139‑1 (MQ=255)                    aaTCACCGACTAAACTCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGTTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTctcc                                                                                                                <  8:7503/139‑1 (MQ=255)                    aaTCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTtctc                                                                                                                 <  7:195157/138‑1 (MQ=255)                    aaTCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTtctc                                                                                                                 <  5:23278/138‑1 (MQ=255)                    aaTCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTctcc                                                                                                                >  3:219208/1‑139 (MQ=255)                     aTCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTctcct                                                                                                               >  4:216972/1‑139 (MQ=255)                      tCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTctcc                                                                                                                <  4:81393/137‑1 (MQ=255)                      tCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTctcc                                                                                                                >  3:81393/1‑137 (MQ=255)                      tCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGGCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTctcctc                                                                                                              <  1:312644/139‑1 (MQ=255)                        aCCGACTAAAATCCTGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTctcctcct                                                                                                            <  3:404433/139‑1 (MQ=255)                        aCCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTctcctcct                                                                                                            <  2:391919/139‑1 (MQ=255)                         ccGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTctcctcctc                                                                                                           <  7:12228/139‑1 (MQ=255)                         ccGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTctcctcctc                                                                                                           <  4:344569/139‑1 (MQ=255)                         ccGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTctcctcctc                                                                                                           <  5:135143/139‑1 (MQ=255)                         ccGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTctcctcctc                                                                                                           <  8:58710/139‑1 (MQ=255)                         ccGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTctcctcctc                                                                                                           <  3:216972/139‑1 (MQ=255)                         ccGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTctcctcctc                                                                                                           <  2:243406/139‑1 (MQ=255)                          cGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGCCTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCa                                                                                                          >  7:40115/1‑139 (MQ=255)                                aaaTCATGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGtt                                                                                                      <  5:217183/137‑1 (MQ=255)                                 aaTCATGTCAACTCCCTCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGATCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTAag                                                                                                   <  1:376102/139‑1 (MQ=255)                                      tGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTagag                                                                                                 >  7:207883/1‑136 (MQ=255)                                      tGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAgaggtg                                                                                              >  2:358564/1‑137 (MQ=255)                                      tGTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAgaggag                                                                                              <  2:53891/139‑1 (MQ=255)                                       gTCAACTCCCGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTagag                                                                                                 >  1:46289/1‑135 (MQ=255)                                       gTCAACTCCCGCTTGTTCAGCAAGATTAGCTGCCGGATAATCATAAGCTGTCAGAATTACAATCGGTTCTTCAGACTCCTAACTTTTTAGAAAATCCAGTTTTGTATTCATCTTTTCTCCTCCTCATGTAAagag                                                                                                 >  6:287427/1‑135 (MQ=255)                                              cccGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTg                                                                                      <  1:275005/139‑1 (MQ=255)                                               ccGCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTATAGGAGATGATCTGc                                                                                     >  7:312686/1‑139 (MQ=255)                                                 gCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCg                                                                                   <  8:359121/139‑1 (MQ=255)                                                 gCTTGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCg                                                                                   <  6:201438/139‑1 (MQ=255)                                                    tGTTCAGCAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGttt                                                                                >  1:53540/1‑139 (MQ=255)                                                    tGTTCAGCAAGTTTAGCTGACGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGttt                                                                                >  1:18863/1‑139 (MQ=255)                                                           cAAGTTTAGCTGCCGGATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCttctg                                                                         >  6:222287/1‑136 (MQ=255)                                                                         ggATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAAcc                                                           >  7:114228/1‑139 (MQ=255)                                                                         ggATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAAcc                                                           <  8:207883/139‑1 (MQ=255)                                                                         ggATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAAc                                                            >  7:404334/1‑138 (MQ=255)                                                                           aTAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCtt                                                         <  2:34129/139‑1 (MQ=255)                                                                               tCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑Gt                                                    >  7:279671/1‑139 (MQ=255)                                                                                    aGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGAGGATCTGCCGTTTTCTTCTGCCAGCCAAAAAACCTTCC‑Gttacaa                                               >  8:327136/1‑135 (MQ=255)                                                                                     gCGGACAGCATGACAATCGGATCTACAGACTCCATCATTTTTAGAAAATCCAGTTTTGTTTTCATGTAATCTCCTCCTCCTGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑Gttacaac                                              <  6:329422/139‑6 (MQ=255)                                                                                        gTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑Gttacaac                                              >  1:320077/1‑131 (MQ=255)                                                                                         tCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTtc                                                                                                                   >  8:302855/1‑67 (MQ=255)                                                                                         tCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTtc                                                                                                                   <  7:302855/67‑1 (MQ=255)                                                                                         tCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGat                                                                                         >  8:343298/1‑92 (MQ=255)                                                                                         tCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGat                                                                                         <  7:343298/93‑2 (MQ=255)                                                                                         tCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAATCCAAAAAACCTTCC‑Gt                                                    >  7:137393/1‑129 (MQ=255)                                                                                         tCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑Gt                                                    <  8:137393/129‑1 (MQ=255)                                                                                          cAGCATGACAATCGGTTCTTCAGACTCCTTCAATTTTAGAAAATCCAGTTTTGTATTCATGTTTTCTCCTCCTCATGTTTAGAGTAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTACC‑GTTACAACgaga                                          >  8:91638/1‑138 (MQ=255)                                                                                           aGCATGACAATCGGTTCTTCAGTCTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGaa                                         <  8:94083/138‑1 (MQ=255)                                                                                           aGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAg                                        >  8:366351/1‑139 (MQ=255)                                                                                                gacaATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGAttc                                   >  8:75287/2‑139 (MQ=255)                                                                                                   aaTCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGAttcttc                                <  1:288103/139‑1 (MQ=255)                                                                                                            ttcagactcCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTATTCTGCAAGCCAAAAAACCATCC‑GTTACAACGAGAAGGATTATTCACTTTCTa                        >  7:343145/7‑138 (MQ=255)                                                                                                                   tcCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCgg                >  4:154231/1‑139 (MQ=255)                                                                                                                     cTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCg              >  8:56943/1‑139 (MQ=255)                                                                                                                       tCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAg            >  3:23296/1‑139 (MQ=255)                                                                                                                        cATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCgg                <  7:170073/134‑1 (MQ=255)                                                                                                                        cATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCgg                >  8:170073/1‑134 (MQ=255)                                                                                                                        cATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAg            <  8:404334/138‑1 (MQ=255)                                                                                                                        cATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAg            <  8:114228/138‑1 (MQ=255)                                                                                                                           ttttAGAAAATCCAGTTTTGTCTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGttt         >  6:193220/1‑138 (MQ=255)                                                                                                                             ttAGACAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCCTGGTTCGAGGAGTTGATCTGCAGTTTTCTTCTGCAAGGCAAATATACTGCC‑GTTACAACGAGAAGGTTTCTTCACTTTCTAAAGTTCGGCGAGTTTCa       <  5:287427/138‑1 (MQ=255)                                                                                                                             ttAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCa       <  8:312686/138‑1 (MQ=255)                                                                                                                             ttAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCa       <  8:279671/138‑1 (MQ=255)                                                                                                                             ttAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCa       <  7:387543/138‑1 (MQ=255)                                                                                                                              tAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCAt      <  8:339511/138‑1 (MQ=255)                                                                                                                              tAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCAt      <  8:40115/138‑1 (MQ=255)                                                                                                                               aGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCCGGTA‑CAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATcc    >  1:45250/1‑139 (MQ=255)                                                                                                                               aGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATcc    >  7:174708/1‑139 (MQ=255)                                                                                                                                gAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATcc    <  5:222287/138‑1 (MQ=255)                                                                                                                                  aaaTCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAATGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCct  <  8:106499/138‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      AGGACGACCATTCCGAGTGAATCACCGACTAAAATCATGTCAACTCCCGCTTGTTCAGCAAGCTTAGCTGCCGGATAATCATAAGCGGTCAGCATCACAATCGGTTCTTCATTCTCCTTCATTTTTAGAAAATCGAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGAGCTGCCGTTTTCTTATACAAGCCAAAAAACCTTCC‑GTT‑TAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCT  >  CP006881/2172377‑2172642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑