Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,172,571	A→C	Y11S (TAT→TCT)	U712_10865 →	Hypothetical Protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,172,571	0	A	C	98.5%	205.6 / ‑6.4	66	Y11S (TAT→TCT)	U712_10865	Hypothetical Protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (30/35);  total (30/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

GGATAATCATAAGCGGTCAGCATCACAATCGGTTCTTCATTCTCCTTCATTTTTAGAAAATCGAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGAGCTGCCGTTTTCTTATACAAGCCAAAAAACCTTCC‑GTT‑TAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTTTTTTGGATCAAGGCAGACTGCTGCAATGTCTATCTATTTTTAT  >  CP006881/2172449‑2172699                                                                                                                           |                                                                                                                                   ggATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAAcc                                                                                                                    >  7:114228/1‑139 (MQ=255) ggATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAAcc                                                                                                                    <  8:207883/139‑1 (MQ=255) ggATAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAAc                                                                                                                     >  7:404334/1‑138 (MQ=255)   aTAATCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCtt                                                                                                                  <  2:34129/139‑1 (MQ=255)       tCATAAGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑Gt                                                                                                             >  7:279671/1‑139 (MQ=255)            aGCGGTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGAGGATCTGCCGTTTTCTTCTGCCAGCCAAAAAACCTTCC‑Gttacaa                                                                                                        >  8:327136/1‑135 (MQ=255)             gCGGACAGCATGACAATCGGATCTACAGACTCCATCATTTTTAGAAAATCCAGTTTTGTTTTCATGTAATCTCCTCCTCCTGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑Gttacaac                                                                                                       <  6:329422/139‑6 (MQ=255)                gTCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑Gttacaac                                                                                                       >  1:320077/1‑131 (MQ=255)                 tCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAATCCAAAAAACCTTCC‑Gt                                                                                                             >  7:137393/1‑129 (MQ=255)                 tCAGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑Gt                                                                                                             <  8:137393/129‑1 (MQ=255)                  cAGCATGACAATCGGTTCTTCAGACTCCTTCAATTTTAGAAAATCCAGTTTTGTATTCATGTTTTCTCCTCCTCATGTTTAGAGTAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTACC‑GTTACAACgaga                                                                                                   >  8:91638/1‑138 (MQ=255)                   aGCATGACAATCGGTTCTTCAGTCTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGaa                                                                                                  <  8:94083/138‑1 (MQ=255)                   aGCATGACAATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAg                                                                                                 >  8:366351/1‑139 (MQ=255)                        gacaATCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGAttc                                                                                            >  8:75287/2‑139 (MQ=255)                           aaTCGGTTCTTCAGACTCCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGAttcttc                                                                                         <  1:288103/139‑1 (MQ=255)                                    ttcagactcCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTATTCTGCAAGCCAAAAAACCATCC‑GTTACAACGAGAAGGATTATTCACTTTCTa                                                                                 >  7:343145/7‑138 (MQ=255)                                           tcCTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCgg                                                                         >  4:154231/1‑139 (MQ=255)                                             cTTCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCg                                                                       >  8:56943/1‑139 (MQ=255)                                               tCATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAg                                                                     >  3:23296/1‑139 (MQ=255)                                                cATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCgg                                                                         <  7:170073/134‑1 (MQ=255)                                                cATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCgg                                                                         >  8:170073/1‑134 (MQ=255)                                                cATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAg                                                                     <  8:404334/138‑1 (MQ=255)                                                cATTTTTAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAg                                                                     <  8:114228/138‑1 (MQ=255)                                                   ttttAGAAAATCCAGTTTTGTCTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGttt                                                                  >  6:193220/1‑138 (MQ=255)                                                     ttAGACAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCCTGGTTCGAGGAGTTGATCTGCAGTTTTCTTCTGCAAGGCAAATATACTGCC‑GTTACAACGAGAAGGTTTCTTCACTTTCTAAAGTTCGGCGAGTTTCa                                                                <  5:287427/138‑1 (MQ=255)                                                     ttAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCa                                                                <  8:312686/138‑1 (MQ=255)                                                     ttAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCa                                                                <  7:387543/138‑1 (MQ=255)                                                     ttAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCa                                                                <  8:279671/138‑1 (MQ=255)                                                      tAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCAt                                                               <  8:40115/138‑1 (MQ=255)                                                      tAGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCAt                                                               <  8:339511/138‑1 (MQ=255)                                                       aGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCCGGTA‑CAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATcc                                                             >  1:45250/1‑139 (MQ=255)                                                       aGAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATcc                                                             >  7:174708/1‑139 (MQ=255)                                                        gAAAATCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATcc                                                             <  5:222287/138‑1 (MQ=255)                                                          aaaTCCAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAATGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCct                                                           <  8:106499/138‑1 (MQ=255)                                                               caGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCTTTTTCTTATGCAAGCCAAAAAACCTTCC‑GTTACATAGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTTTcc                                                     <  7:91638/138‑1 (MQ=255)                                                                 gttttgttttCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCa                                                   <  7:294615/139‑1 (MQ=255)                                                                  ttttgttttCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAg                                                  <  5:414481/139‑1 (MQ=255)                                                                    ttgttttCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGt                                                 <  2:150847/138‑1 (MQ=255)                                                                     tgttttCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTc                                                <  1:143355/138‑1 (MQ=255)                                                                           cctGTTTTCTCCTCCTCCTGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCttttt                                          <  1:358564/136‑1 (MQ=255)                                                                            aTGTTTTCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTTTTTTg                                        >  7:165031/1‑139 (MQ=255)                                                                               ttttCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTTTTTTGGAt                                     <  4:219208/139‑1 (MQ=255)                                                                               tattCTCCTCCTCATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTTTTTTGGa                                      <  4:55270/136‑1 (MQ=255)                                                                                      ctcctcATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTTTTTTGGATCAAGGCa                              <  7:272513/139‑1 (MQ=255)                                                                                      ctcctcATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTTTTTTGGATCAAGGCa                              <  5:404802/139‑1 (MQ=255)                                                                                        cctcATGTTTAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTTTTTTGGATCAAGGCAGa                            <  4:379848/139‑1 (MQ=255)                                                                                               tttAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTTTTTTGGATCAAGGCAGActgctg                      <  3:336101/138‑1 (MQ=255)                                                                                               tttAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTTTTTTGGATCAAGGCAGActgctg                      <  3:329946/138‑1 (MQ=255)                                                                                               tttAGAGGAGATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTTTTTTGGATCAAGGCAGACtgctgc                     <  8:174708/139‑1 (MQ=255)                                                                                                 tAGAGGAGATGATCTGCCGTTTTCTTCAGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTTTTTTGGATCAAGGCAGACTGCTGCa                    <  7:366351/138‑1 (MQ=255)                                                                                                   gaggagATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTTTTTTGGATCAAGGCAGACTGCTGCAAt                  <  2:46289/138‑1 (MQ=255)                                                                                                   gaggagATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTTTTTTGGATCAAGGCAGACTGCTGCAATg                 >  5:375386/1‑139 (MQ=255)                                                                                                   gaggagATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTTTTTTGGATCAAGGCAGACTGCTGCAATg                 >  1:41605/1‑139 (MQ=255)                                                                                                    aggagATGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTTTTTTGGATCAAGGCAGACTGCTGCAATGt                >  6:405318/1‑139 (MQ=255)                                                                                                        gatgatCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTTTTTTGGATCAAGGCAGACTGCTGCAATGtctat            >  5:142828/1‑139 (MQ=255)                                                                                                        gatgatCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTTTTTTGGATCAAGGCAGACTGCTGCAATGtctat            <  6:383773/139‑1 (MQ=255)                                                                                                         aTGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTTTTTTGGATCAAGGCAGACTGCTGCAATGtctatc           >  1:225266/1‑139 (MQ=255)                                                                                                         aTGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTTTTTTGGATCAAGGCAGACTGCTGCAATGtctatc           >  4:201288/1‑139 (MQ=255)                                                                                                         aTGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTTTTTTGGATCAAGGCAGACTGCTGCAATGtctatc           >  3:222990/1‑139 (MQ=255)                                                                                                         aTGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTTTTTTGGATCAAGGCAGACTGCTGCAATGtctatc           >  1:69177/1‑139 (MQ=255)                                                                                                         aTGATCTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTGTTTTGGATCAAGGCAGACTGCTGCAATGtctatc           >  7:348065/1‑139 (MQ=255)                                                                                                              cTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTTTTTTGGATCAAGGCa                              >  4:316748/1‑115 (MQ=255)                                                                                                              cTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTTTTTTGGATCAAGGCa                              <  3:316748/115‑1 (MQ=255)                                                                                                              cTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTTTTTTGGATCAAGGCAGACTGCTGCAATGTCTTTCTAttt      >  1:442336/1‑139 (MQ=255)                                                                                                              cTGCCGTTTTCTTCTGCAAGCCAAAAAACCTTAC‑GTTACAACGAGAATGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCACACTGTCACAGTCCTTTTTTGGATCAATTCAGACTGCTGCAATGTATATCTAttt      >  6:388832/1‑139 (MQ=255)                                                                                                                  cGTTTTCTTCTGCAAGCCAAAAAACCTTCC‑GTTACAACTAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTTTTTTGGATCAAGGCAGACTGCTGCAATGTCTATCTATTTTaat  <  5:388832/139‑1 (MQ=255)                                                                                                                           |                                                                                                                                   GGATAATCATAAGCGGTCAGCATCACAATCGGTTCTTCATTCTCCTTCATTTTTAGAAAATCGAGTTTTGTTTTCATGTTTTCTCCTCCTCATGTTTAGAGGAGATGAGCTGCCGTTTTCTTATACAAGCCAAAAAACCTTCC‑GTT‑TAACGAGAAGGATTCTTCACTTTCTAAAGTTCGGCGAGTTTCATCCCTCTGTCCCAGTCCTTTTTTGGATCAAGGCAGACTGCTGCAATGTCTATCTATTTTTAT  >  CP006881/2172449‑2172699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑