Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,183,157 G→A S124S (TCC→TCT)  U712_10935 ← Uncharacterized protein ypiF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,183,1570GA98.1% 319.4 / ‑3.0 107S124S (TCC→TCT) U712_10935Uncharacterized protein ypiF
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (53/51);  total (55/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01

Only 100 of 111 total aligned reads displayed.

TGAATCTCTCAGTAAGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATTAGGTGCTCCACAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCTAATATTTTTCTTTTTAAGGAGTCATTTAAATGCTCTAACGGAACAGACGGTGTAACAATGATTTTGCCTAACGCATCCTCGCTTTTCCAATTTTCATCAGACGTGAGCAGCACAACATGCGGAAATTCTGATATAAGCTCTTCCCGCAA  >  CP006881/2183022‑2183288
                                                                                                                                       |                                                                                                                                   
tGAATCTCTCAGCAAGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAagag                                                                                                                                  >  8:208627/1‑139 (MQ=255)
tGAATCTCTCAGCAAGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGa                                                                                                                                   <  2:255757/138‑1 (MQ=255)
tGAATCTCTCAGCAAGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGa                                                                                                                                   <  6:418295/138‑1 (MQ=255)
tGAATCTCTCAGCAAGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAAAATTTTTCTTTTTAAagag                                                                                                                                  >  8:416924/1‑139 (MQ=255)
 gAATCTCTCAGCAAGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGt                                                                                                                                 >  8:409477/1‑139 (MQ=255)
  aaTCTCTCAGCAAGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGt                                                                                                                                 >  8:105845/1‑138 (MQ=255)
    tctctcAGCAAGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCAt                                                                                                                              >  7:260153/1‑139 (MQ=255)
    tctatcAGCAAGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGATCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCAt                                                                                                                              >  5:78290/1‑139 (MQ=255)
      tctcAGCAAGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCAtt                                                                                                                             >  5:170030/1‑138 (MQ=255)
      tctcAGCAAGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCAtt                                                                                                                             >  1:153970/1‑138 (MQ=255)
      tctcAGCAAGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTATTTAAAGAGTCAtt                                                                                                                             >  1:337926/1‑138 (MQ=255)
       ctcAGCAAGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCAttt                                                                                                                            >  8:395259/1‑138 (MQ=255)
       ctcAGCAAGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGAGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAAGATTTTTCTTTTTAAAGAGTCAttt                                                                                                                            >  6:212798/1‑138 (MQ=255)
        tcAGCAAGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTa                                                                                                                           >  3:277170/1‑138 (MQ=255)
        tcAGCAAGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTa                                                                                                                           >  4:411194/1‑138 (MQ=255)
        tcAGCAAGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTa                                                                                                                           >  2:139651/1‑138 (MQ=255)
        tcAGCAAGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTa                                                                                                                           >  6:387505/1‑138 (MQ=255)
        tcAGCAAGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTa                                                                                                                           >  8:68669/1‑138 (MQ=255)
          agcaaGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTaaa                                                                                                                         <  6:170030/135‑1 (MQ=255)
          agcaaGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTaaa                                                                                                                         <  7:395259/135‑1 (MQ=255)
          agcaaGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTaaa                                                                                                                         <  5:388065/135‑1 (MQ=255)
          agcaaGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTaaa                                                                                                                         <  8:429565/135‑1 (MQ=255)
          agcaaGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTaaa                                                                                                                         <  5:118705/135‑1 (MQ=255)
          agcaaGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTaaa                                                                                                                         <  1:139651/135‑1 (MQ=255)
          agcaaGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTaaa                                                                                                                         <  1:407305/135‑1 (MQ=255)
          agcaaGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTaa                                                                                                                          <  2:65667/134‑1 (MQ=255)
          agcaaGGTCAATATAGCCAAAATATTGATTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTaa                                                                                                                          <  6:28184/134‑1 (MQ=255)
               ggTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAACACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGACATTTACATGctc                                                                                                                    >  2:354288/1‑138 (MQ=255)
               ggTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGctc                                                                                                                    >  1:234672/1‑138 (MQ=255)
               ggTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATATAAATGctc                                                                                                                    >  3:246366/1‑138 (MQ=255)
                  cAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTaat                                                                                                                >  6:189907/1‑138 (MQ=255)
                         gCCAAAATATTGTTTATAATGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACa                                                                                                          <  7:73292/138‑1 (MQ=255)
                         gCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACa                                                                                                          <  1:38916/138‑1 (MQ=255)
                          ccAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATTAGGTGCTCCACAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCTAATATTTTTCTTTTTAAGGAGTCATTTAAATGCTCTAACGGAACAGa                                                                                                        >  1:425726/1‑139 (MQ=255)
                          ccAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAATGAGTCATTTATATGCTCTAATGGAACAGa                                                                                                        >  3:230456/1‑139 (MQ=255)
                          ccAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTaa                                                                                                                 >  8:235621/1‑130 (MQ=255)
                          ccAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTaa                                                                                                                 <  7:235621/130‑1 (MQ=255)
                          ccAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGa                                                                                                        >  5:164124/1‑139 (MQ=255)
                          ccAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGa                                                                                                        <  5:296337/139‑1 (MQ=255)
                          ccAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGa                                                                                                        >  4:438127/1‑139 (MQ=255)
                          ccAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGa                                                                                                        <  2:234672/139‑1 (MQ=255)
                          ccAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGa                                                                                                        >  7:337489/1‑139 (MQ=255)
                          ccAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGa                                                                                                        >  1:300444/1‑139 (MQ=255)
                                     tttATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACaa                                                                                             <  2:145653/139‑1 (MQ=255)
                                     tttATAAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACa                                                                                              <  7:170879/138‑1 (MQ=255)
                                       tataAGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAAt                                                                                            >  6:122176/1‑138 (MQ=255)
                                           aGGAACTGTAAGTGTTTTTATAAAGTGCTCCATAACAGTAACAAAACGTTCAAAATATCAGCATTGCGTTCATACAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCAta                                                                                        <  4:286419/138‑2 (MQ=255)
                                           aGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCAtt                                                                                        <  6:42157/138‑1 (MQ=255)
                                           aGGAAATGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCAtt                                                                                        <  3:446553/138‑1 (MQ=255)
                                               aaTGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCAACCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGcc                                                                                   >  7:260783/1‑139 (MQ=255)
                                                aTGTAAGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCt                                                                                  <  7:409477/139‑1 (MQ=255)
                                                   tAAGTGTTTTTATTAGGTGCTCCACAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCTAATATTTTTCTTTTTAAGGAGTCATTTAAATGCTCTAACGGAACAGACGGTGTAACAATGATTTTGCCTaa                                                                                <  2:425726/138‑1 (MQ=255)
                                                     aGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGc                                                                             <  2:408681/139‑1 (MQ=255)
                                                     aGTGTTTTTATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGc                                                                             <  2:75293/139‑1 (MQ=255)
                                                         tttttATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATc                                                                          <  4:873/138‑1 (MQ=255)
                                                           tttATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCt                                                                        >  3:397006/1‑138 (MQ=255)
                                                           tttATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCt                                                                        >  5:336794/1‑138 (MQ=255)
                                                           tttATAAAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAATGCGTTCATCCAATATTTTTCTTTTTAAagag                                                                                                                                  <  8:389248/80‑1 (MQ=255)
                                                            ttataaAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTaaa                                                                                                                         >  2:286165/1‑88 (MQ=255)
                                                            ttataaAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTaaa                                                                                                                         <  1:286165/88‑1 (MQ=255)
                                                            ttataaAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTc                                                                       <  4:277170/138‑1 (MQ=255)
                                                            ttataaAGTGCTCCATAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTc                                                                       <  2:300444/138‑1 (MQ=255)
                                                                           taaCTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTaa                                                                                                                 >  3:182454/2‑81 (MQ=255)
                                                                           taaCTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTaa                                                                                                                 <  4:182454/80‑1 (MQ=255)
                                                                             aCTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTtcgtc                                                     <  3:438127/139‑1 (MQ=255)
                                                                             aCTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTtcgt                                                      <  7:277437/138‑3 (MQ=255)
                                                                                gTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTtcgt                                                      >  1:304286/1‑133 (MQ=255)
                                                                                 tAACAAAACGTTCAAAATTTCATCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTATCGTCAGat                                                 <  2:361792/139‑2 (MQ=255)
                                                                                 tAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCAtt                                                                                        >  1:270700/1‑100 (MQ=255)
                                                                                 tAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGat                                                 >  1:226690/1‑138 (MQ=255)
                                                                                 tAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGat                                                 >  8:360552/1‑138 (MQ=255)
                                                                                 tAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGat                                                 <  8:337489/139‑2 (MQ=255)
                                                                                 tAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGat                                                 <  5:331648/139‑2 (MQ=255)
                                                                                 tAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGat                                                 <  7:68669/139‑2 (MQ=255)
                                                                                 tAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGat                                                 >  7:318327/1‑138 (MQ=255)
                                                                                  aaCAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAAc                                                                               <  6:241492/108‑1 (MQ=255)
                                                                                  aaCAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAAc                                                                               <  8:295193/108‑1 (MQ=255)
                                                                                  aaCAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAAc                                                                               >  5:241492/1‑108 (MQ=255)
                                                                                  aaCAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAAc                                                                               >  7:295193/1‑108 (MQ=255)
                                                                                   aCAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGt                                               >  1:133236/1‑139 (MQ=255)
                                                                                   aCAAAACGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTAATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGt                                               >  5:58197/1‑139 (MQ=255)
                                                                                   aCAAAACGTTCAAAATTTCAGCAGTGCGGTCATCCAAGATTTTTCTTTTTAAAGAGTCATTTAATTGCTCTAATGGATCAGACGAGGTACGCATCATTTTGCCTATCGCATCCTCCGGTCTCCACGTTTCGTCAGATGt                                               >  2:404460/1‑139 (MQ=255)
                                                                                      aaaCGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGag                                            <  5:189907/139‑1 (MQ=255)
                                                                                      aaaCGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGag                                            <  7:257877/139‑1 (MQ=255)
                                                                                      aaaCGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGag                                            <  6:161753/139‑1 (MQ=255)
                                                                                      aaaCGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGag                                            <  7:189483/139‑1 (MQ=255)
                                                                                       aaCGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCACCAACTTTCGTCAGATGTGag                                            >  6:434094/1‑138 (MQ=255)
                                                                                         cGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCAtt                                                                                        >  7:238216/1‑92 (MQ=255)
                                                                                         cGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCAtt                                                                                        <  8:238216/92‑1 (MQ=255)
                                                                                         cGTTCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGagcag                                         >  7:430100/1‑139 (MQ=255)
                                                                                           ttCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGagcagga                                       >  4:76220/1‑137 (MQ=255)
                                                                                           ttCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGagcagga                                       >  3:382316/1‑137 (MQ=255)
                                                                                           ttCAAAATTTCAGCAGTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTAATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGagcagga                                       <  6:58197/139‑3 (MQ=255)
                                                                                                     cagcagTGCGTTCATCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCg                              >  6:17379/1‑138 (MQ=255)
                                                                                                                  aTCCAATATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGttat                 <  2:153970/138‑1 (MQ=255)
                                                                                                                     caaTATTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGc             >  3:165665/2‑139 (MQ=255)
                                                                                                                       atatTTTTCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCAGTTATCAGctc           >  6:176230/1‑139 (MQ=255)
                                                                                                                            tttCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTccc      >  4:198141/1‑139 (MQ=255)
                                                                                                                            tttCTTTTTAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTccc      >  1:151090/1‑139 (MQ=255)
                                                                                                                                 ttttAAAGAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATGCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCaa  <  5:416667/138‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                   
TGAATCTCTCAGTAAGGTCAATATAGCCAAAATATTGTTTATAAGGAAATGTAAGTGTTTTTATTAGGTGCTCCACAACTGTAACAAAACGTTCAAAATTTCAGCAGTGCGTTCATCTAATATTTTTCTTTTTAAGGAGTCATTTAAATGCTCTAACGGAACAGACGGTGTAACAATGATTTTGCCTAACGCATCCTCGCTTTTCCAATTTTCATCAGACGTGAGCAGCACAACATGCGGAAATTCTGATATAAGCTCTTCCCGCAA  >  CP006881/2183022‑2183288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑