Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,183,293	C→T	R79K (AGG→AAG)	U712_10935 ←	Uncharacterized protein ypiF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,183,293	0	C	T	100.0%	281.0 / NA	89	R79K (AGG→AAG)	U712_10935	Uncharacterized protein ypiF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/58);  total (31/58)

GGAGTCATTTAAATGCTCTAACGGAACAGACGGTGTAACAATGATTTTGCCTAACGCATCCTCGCTTTTCCAATTTTCATCAGACGTGAGCAGCACAACATGCGGAAATTCTGATATAAGCTCTTCCCGCAAATCCCTAAGTCCTTGAACGGTTATTTCATTTCTATCAACATATGTATATGGGGGCAGTAAATATACCCGTCCCTTTAACTGCCTTTCCAGTTCTTCTGACAGAAGCTGCGTGAATTCGCCTTTCTCCGCCGCCATTT  >  CP006881/2183157‑2183425                                                                                                                                         |                                                                                                                                     agAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTa                                                                                                                                    <  5:176230/138‑1 (MQ=255) agAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTa                                                                                                                                    <  7:360552/138‑1 (MQ=255) agAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTa                                                                                                                                    <  6:336794/138‑1 (MQ=255) agAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTa                                                                                                                                    <  2:133236/138‑1 (MQ=255)  gAGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTaa                                                                                                                                   <  5:48506/139‑1 (MQ=255)   aGTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAg                                                                                                                                  <  2:402990/139‑1 (MQ=255)    gTCATTTAAATGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAg                                                                                                                                  <  3:76220/138‑1 (MQ=255)          tAAAAGCTCTAATGGAACAGACGATGTAACAATCAATTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTg                                                                                                                            <  2:337926/138‑1 (MQ=255)           aaaTTCTCTACTGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGTACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGa                                                                                                                           <  8:430105/138‑1 (MQ=255)           aaaTGCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGa                                                                                                                           <  8:430100/138‑1 (MQ=255)               gCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCATATGTGAGCAGGACAACATGCGGAAATTCCTTTATCAGCTCTACCCGCAAATCCTTAAGCCCTTTAACGGt                                                                                                                      >  6:290677/1‑139 (MQ=255)               gCTCTAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGt                                                                                                                      >  2:192723/1‑139 (MQ=255)                ctctAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAACTTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGt                                                                                                                      >  2:106618/1‑138 (MQ=255)                   tAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTa                                                                                                                                    >  1:423440/1‑121 (MQ=255)                   tAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTa                                                                                                                                    <  2:423440/121‑1 (MQ=255)                   tAATGGAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTatt                                                                                                                  >  3:193593/1‑139 (MQ=255)                      tggAACAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTatttca                                                                                                               <  8:276332/138‑1 (MQ=255)                         aaCAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTatttcattt                                                                                                            <  5:387505/139‑1 (MQ=255)                           cAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGTAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCt                                                                                                          >  6:40057/1‑139 (MQ=255)                           cAGACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTatttcatttc                                                                                                           >  7:25190/1‑138 (MQ=255)                             gACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTAt                                                                                                        <  2:304286/139‑1 (MQ=255)                             gACGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTAt                                                                                                        <  8:307295/139‑1 (MQ=255)                              aCGATGTAACAATCCTTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGGTATTTCATTTCTATc                                                                                                       <  4:230456/139‑1 (MQ=255)                              aCGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATc                                                                                                       <  4:382316/139‑1 (MQ=255)                              aCGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATc                                                                                                       <  8:25190/139‑1 (MQ=255)                              aCGATGTAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATc                                                                                                       <  6:164124/139‑1 (MQ=255)                                   gtAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACa                                                                                                   >  7:272689/1‑138 (MQ=255)                                    tAACAATCATTTTGCCTAACTCATCCTCAGCTCTACAACTTTCGTCAGATGTGAGCAGGACTACATGCTGAAAATCAGTTATAAGCGCTTCCCGATAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAAGat                                                                                                  <  6:42062/138‑1 (MQ=255)                                    tAACAATCATTTTGCCTAACGGATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCACGAAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACat                                                                                                  <  8:88489/138‑1 (MQ=255)                                    tAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACat                                                                                                  <  4:397006/138‑1 (MQ=255)                                    tAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACat                                                                                                  <  7:121180/138‑1 (MQ=255)                                    tAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACat                                                                                                  <  6:51353/138‑1 (MQ=255)                                    tAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACat                                                                                                  <  3:156393/138‑1 (MQ=255)                                    tAACAATCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACat                                                                                                  <  5:224643/138‑1 (MQ=255)                                        aaTCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGCACGGTTATTTCATTTCTATAAACATATGta                                                                                             >  4:250285/1‑139 (MQ=255)                                        aaTCATTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGta                                                                                             <  7:416924/139‑1 (MQ=255)                                          tcaTTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTatttca                                                                                                               <  5:97013/117‑1 (MQ=255)                                          tcaTTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTatttca                                                                                                               >  6:97013/3‑119 (MQ=255)                                          tcaTTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATc                                                                                                       <  2:425987/125‑1 (MQ=255)                                          tcaTTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATc                                                                                                       >  1:425987/3‑127 (MQ=255)                                            aTTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTatttcattt                                                                                                            >  4:68068/1‑120 (MQ=255)                                            aTTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTatttcattt                                                                                                            <  3:68068/120‑1 (MQ=255)                                            aTTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGtat                                                                                            >  1:331435/1‑136 (MQ=255)                                            aTTTTGCCTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGtat                                                                                            <  2:331435/136‑1 (MQ=255)                                             ttttGCCTAACGCATCCTCAGCTCTACAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAgg                                                                                        <  5:212798/139‑1 (MQ=255)                                                  ccTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAggcggaa                                                                                   <  2:420980/139‑3 (MQ=255)                                                  ccTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAggcggaa                                                                                   <  7:105845/139‑3 (MQ=255)                                                  ccTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAggcggaa                                                                                   <  2:37657/139‑3 (MQ=255)                                                  ccTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAggcggaa                                                                                   <  5:58953/139‑3 (MQ=255)                                                  ccTAACGCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAggcggaa                                                                                   <  5:17379/139‑3 (MQ=255)                                                  ccTAACGCATCCTCAGCTCACCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAggcggaa                                                                                   <  5:434094/139‑3 (MQ=255)                                                        gCATCCTCAGCTCTCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAat                                                                             <  5:40057/139‑1 (MQ=255)                                                                agctctCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATAccc                                                                       <  8:260783/132‑1 (MQ=255)                                                                agctctCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATAccc                                                                       <  1:192723/132‑1 (MQ=255)                                                                agctctCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGt                                                                     <  2:151090/134‑1 (MQ=255)                                                                agctctCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGt                                                                     <  6:119896/134‑1 (MQ=255)                                                                agctctCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGt                                                                     >  7:10656/6‑139 (MQ=255)                                                                agctctCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCAGt                                                                     >  2:374840/6‑139 (MQ=255)                                                                 gctctCCAACTTTCGTGAGATGTGAGCATGACAACATTCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTc                                                                    <  2:275337/135‑1 (MQ=255)                                                                  ctctCCAACTTTCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTcc                                                                   >  7:273481/4‑139 (MQ=255)                                                                         actttCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAAc                                                            <  5:28070/137‑1 (MQ=255)                                                                          ctttCGTCAGATGTGAGCAGGACAACATGCTGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACt                                                           <  6:78290/138‑1 (MQ=255)                                                                            ttCGTCAGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCTTCCCTTTAACTGc                                                         >  1:275337/1‑139 (MQ=255)                                                                               gtcaGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACa                                                                                                   <  4:39384/93‑1 (MQ=255)                                                                               gtcaGATGTGAGCAGGACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACa                                                                                                   >  3:39384/2‑94 (MQ=255)                                                                               gtcaGATGAGAGCAGGACTACATGCGGAAATTCCGTTATCAGCTCTTACCGCAAATCCTTATGCCCTTGAACGGTTAATTCATTTCTATCAACATATGTATAAGGCGGAAGAAAATATACCCGTCCCTTTAACTGCCta                                                      <  2:35025/138‑2 (MQ=255)                                                                                         agcaggACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTcaa                                                  <  4:20181/133‑3 (MQ=255)                                                                                         agcaggACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTcaa                                                  >  3:20181/1‑131 (MQ=255)                                                                                         agcaggACAACATGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGttctt                                            >  2:337727/1‑139 (MQ=255)                                                                                             ggacaacaTGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTATCTGCCTTTCAAGTtcttct                                          >  7:161498/3‑137 (MQ=255)                                                                                              gacaacaTGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACat                                                                                                  >  4:199102/2‑80 (MQ=255)                                                                                              gacaacaTGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACat                                                                                                  <  3:199102/79‑1 (MQ=255)                                                                                              gacaacaTGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTATATCAACATATGTATAAGGCGGAAGTAAATATACACGTCCCTTTAACTGCCTTTCAAGTTCTTCTGa                                        >  1:423232/2‑138 (MQ=255)                                                                                                caacaTGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTatttcat                                                                                                              <  5:246126/66‑1 (MQ=255)                                                                                                caacaTGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTatttcat                                                                                                              >  6:246126/1‑66 (MQ=255)                                                                                                   caTGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACat                                                                                                  <  7:240606/75‑1 (MQ=255)                                                                                                   caTGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACat                                                                                                  >  8:240606/1‑75 (MQ=255)                                                                                                   caTGCGGAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAg                                  <  5:20836/139‑1 (MQ=255)                                                                                                        ggAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACat                                                                                                  <  4:129213/70‑1 (MQ=255)                                                                                                        ggAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACat                                                                                                  >  3:129213/1‑70 (MQ=255)                                                                                                        ggAAATTCCGTTATCAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCg                             >  2:52079/1‑139 (MQ=255)                                                                                                        ggAAATTCCGTTATCAGCTCTGCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCGTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCg                             >  3:228603/1‑139 (MQ=255)                                                                                                               ccgttatcAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTaaact                       <  3:183574/134‑3 (MQ=255)                                                                                                               ccgttatcAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTaaact                       <  8:318327/134‑3 (MQ=255)                                                                                                                              cccGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCcgccgc       >  1:41089/1‑139 (MQ=255)                                                                                                                               ccGCATATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCcgccgc       <  3:361388/138‑1 (MQ=255)                                                                                                                                cGCAAATCCTTCAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCa     <  4:165665/139‑1 (MQ=255)                                                                                                                                  cAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCAtt   >  8:380737/1‑139 (MQ=255)                                                                                                                                   aaaTCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCAttt  >  5:14161/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                     GGAGTCATTTAAATGCTCTAACGGAACAGACGGTGTAACAATGATTTTGCCTAACGCATCCTCGCTTTTCCAATTTTCATCAGACGTGAGCAGCACAACATGCGGAAATTCTGATATAAGCTCTTCCCGCAAATCCCTAAGTCCTTGAACGGTTATTTCATTTCTATCAACATATGTATATGGGGGCAGTAAATATACCCGTCCCTTTAACTGCCTTTCCAGTTCTTCTGACAGAAGCTGCGTGAATTCGCCTTTCTCCGCCGCCATTT  >  CP006881/2183157‑2183425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑