Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,184,629 T→G A279A (GCA→GCC)  U712_10945 ← TPR repeat‑containing protein ypiA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,184,6290TG99.2% 383.4 / ‑4.7 119A279A (GCA→GCC) U712_10945TPR repeat‑containing protein ypiA
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (56/62);  total (56/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.09e-01

Only 100 of 119 total aligned reads displayed.

TGTTCATAATCCTCTTCTTTATGATACACGGCAAGAAGTGTATGAAGTGCCTCGACGAAGCCCGGATCAAGTGCAAGCGCCTCCTGAAGCAGCTGTTTCCCATCTTCTGACTTCCCGATTTTTAAAGCCATTTTTGCGGCATAGAGGAAAAGCTCTTTGTTATATTCATCATATCTGATACCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATCCCTTCAGCTTCATAGCTTTTCGAAAGCGGCATATAAAGTGAAGAATAAGA  >  CP006881/2184495‑2184760
                                                                                                                                      |                                                                                                                                   
tgatCATAATCCTCTTCTTTGTGATACACGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTAGGCgg                                                                                                                                 >  3:269219/4‑139 (MQ=255)
tgatCATAATCCTCTTCTTTGTGATACACGGCAAGAAGTGCATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCgg                                                                                                                                 >  6:281785/4‑139 (MQ=255)
  atCATAATCCTCTTCTTTGTGATACACGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCa                                                                                                                               >  2:331996/2‑139 (MQ=255)
  atCATAATCCTCTTCTTTGTGATACACGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCAGATTTTCAAGGCCATTTTGGCGGCa                                                                                                                               >  2:335661/2‑139 (MQ=255)
  aacATAAAACTCTTCTATGTGATACACGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAACCAGCTTCTTCCCCACTTCTGACTTCCCGATAATCAAGGCCATTTTGGcctc                                                                                                                                >  4:395378/3‑135 (MQ=255)
       aaTCCTCTTCTTTGTGATACACGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATaaag                                                                                                                          >  7:84518/1‑139 (MQ=255)
       aaTCCTCTTCTTTGTGATACACGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATaaag                                                                                                                          >  1:390073/1‑139 (MQ=255)
            tcttctTTGTGATACACGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGaa                                                                                                                       >  1:169791/1‑137 (MQ=255)
              ttcttTGTGATACACGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAgt                                                                                                                   >  1:173824/1‑138 (MQ=255)
                cttTGTGATACACGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAgtt                                                                                                                  <  1:155976/138‑3 (MQ=255)
                 tttGTGATACACGGCATGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGGTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGttct                                                                                                                <  7:424916/139‑1 (MQ=255)
                  ttgtGATAGACGGCAAGTAGTGTATGACGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGttct                                                                                                                <  4:269219/135‑1 (MQ=255)
                   tgtGATACACGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGttct                                                                                                                <  6:428500/135‑1 (MQ=255)
                   tgtGATACACGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGttct                                                                                                                <  1:331996/135‑1 (MQ=255)
                   tgtGATACACGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGttct                                                                                                                <  1:26531/135‑1 (MQ=255)
                   tgtGATACACGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGttct                                                                                                                <  4:61902/135‑1 (MQ=255)
                   tgtGATACACGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCttt                                                                                                              >  1:358401/3‑139 (MQ=255)
                   tgtGATACACGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCttt                                                                                                              >  8:389148/3‑139 (MQ=255)
                   tgtGATACACGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCttt                                                                                                              >  4:392644/3‑139 (MQ=255)
                   tgtGATACACGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCttt                                                                                                              >  3:279970/3‑139 (MQ=255)
                     tGATACACGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTg                                                                                                             <  5:279078/138‑1 (MQ=255)
                     tGATACACGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTg                                                                                                             <  2:358401/138‑1 (MQ=255)
                       aTACACGGCAAGAAGTGTATTAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAACCAGCATCTTCCCCTCATCTGACTTCCCGATTTTCAATGCCATTTTGGCGGCATAAATGAAAAGTTCtttttt                                                                                                           >  2:155976/1‑138 (MQ=255)
                       aTACACGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGtt                                                                                                           <  1:144671/138‑1 (MQ=255)
                       aTACACGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACGTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTta                                                                                                          >  2:335675/1‑139 (MQ=255)
                        tACACGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTta                                                                                                          >  5:432590/1‑138 (MQ=255)
                        tACACGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTta                                                                                                          >  7:325552/1‑138 (MQ=255)
                         acacGGCAAGAAGTGTAGGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTtata                                                                                                        <  6:161007/139‑1 (MQ=255)
                          ctcGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTtata                                                                                                        <  5:91966/136‑1 (MQ=255)
                          cacGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTtata                                                                                                        <  5:172557/138‑1 (MQ=255)
                          cacGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTtata                                                                                                        <  8:15591/138‑1 (MQ=255)
                          cacGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTtata                                                                                                        <  4:274149/138‑1 (MQ=255)
                          cacGGCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTatat                                                                                                       >  4:153469/1‑139 (MQ=255)
                             ggCAAGAGGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTACCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTAatatac                                                                                                     >  3:218665/1‑136 (MQ=255)
                             ggCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAgtt                                                                                                                  <  2:61778/125‑3 (MQ=255)
                             ggCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAgtt                                                                                                                  >  1:61778/1‑123 (MQ=255)
                              gCAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATtcat                                                                                                   <  6:394442/139‑1 (MQ=255)
                               cAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATtcat                                                                                                   >  8:419186/1‑138 (MQ=255)
                               cAAGAAGTGTATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATtcat                                                                                                   >  5:190837/1‑138 (MQ=255)
                                      tgtATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTg                                                                                           <  5:61217/139‑1 (MQ=255)
                                       gtATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTGCCGATTTTCAAGGCCCTTTTGGCGTCATAAAGGAAAAGTTCTTTGTAATATTCATCATATCTGa                                                                                          >  3:432506/1‑139 (MQ=255)
                                        tATGAAGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGAt                                                                                         >  5:35821/1‑139 (MQ=255)
                                          tGAAGTGCCTCGACGAAGCCCGGATCAAGTGCAAGCGCCTCCTGAAGCAGCTGTTTCCCATCTTCTGACTTCCCGATTTTTAAAGCCATTTTTGCGGCATAGAGGAAAAGCTCTTTGTTATATTCATCATATCTGATa                                                                                        <  7:390151/138‑1 (MQ=255)
                                            aaGCGCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATccc                                                                                      <  5:33500/138‑1 (MQ=255)
                                             agcgCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATtcat                                                                                                   <  6:441616/121‑1 (MQ=255)
                                             agcgCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATtcat                                                                                                   >  5:441616/4‑124 (MQ=255)
                                             agcgCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCct                                                                                    >  7:254009/4‑139 (MQ=255)
                                              gcgCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCctc                                                                                   >  7:216032/3‑139 (MQ=255)
                                                gCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGtt                                                                                                           >  7:363579/1‑113 (MQ=255)
                                                gCCTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGtt                                                                                                           <  8:363579/113‑1 (MQ=255)
                                                  cTCGACAAAGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCtttt                                                                               >  6:200098/1‑139 (MQ=255)
                                                          aGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCGTTTTGGCGGCATAAAGGAAAAGTTCTTTGTtata                                                                                                        <  7:224783/106‑1 (MQ=255)
                                                          aGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCGTTTTGGCGGCATAAAGGAAAAGTTCTTTGTtata                                                                                                        >  8:224783/1‑106 (MQ=255)
                                                          aGCCCGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGtttt                                                                       >  7:181719/1‑139 (MQ=255)
                                                            cccGGATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGttttt                                                                      >  4:209365/1‑138 (MQ=255)
                                                               ggATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATg                                                                  >  7:177759/1‑139 (MQ=255)
                                                               ggATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATg                                                                  <  6:414311/139‑1 (MQ=255)
                                                               ggATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCCTCCCCTCTTCTGACTTCCCGATTATCAAGGCCAATTTGGCGGCATATAGGAAAAGTTCTATGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATg                                                                  <  1:183455/139‑1 (MQ=255)
                                                                gATCAAGCGCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGc                                                                 <  4:215357/139‑1 (MQ=255)
                                                                     agcgCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGAtc                                                                                        <  7:323718/108‑2 (MQ=255)
                                                                      gcgCAAGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCtt                                                            >  3:75359/3‑138 (MQ=255)
                                                                           aGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACa                                                      <  6:35821/139‑1 (MQ=255)
                                                                           aGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACa                                                      <  8:254009/139‑1 (MQ=255)
                                                                           aGCGCCTCCTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACa                                                      <  3:392644/139‑1 (MQ=255)
                                                                           aGCGCCTACTGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCGATTTTGGCGGCATAAAGGAAAAGTTCTGTGTTATATTCATCATATCTGATCCACTCTTTTGCAGATTTTAATGCCTCTTCATAc                                                       >  6:138193/1‑138 (MQ=255)
                                                                                 tcctGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCt                                                 >  5:3604/1‑138 (MQ=255)
                                                                                 tcctGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCt                                                 >  3:252884/1‑138 (MQ=255)
                                                                                 tcctGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTAGATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCtt                                                >  5:279585/1‑139 (MQ=255)
                                                                                  cctGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTc                                               <  8:68250/139‑1 (MQ=255)
                                                                                   ctGAAGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCtt                                                <  8:400757/137‑1 (MQ=255)
                                                                                      aaGCAGCTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCt                                           >  5:200035/1‑139 (MQ=255)
                                                                                        gcagcTTCTTCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCtt                                          >  8:146108/1‑138 (MQ=255)
                                                                                           gcttcttCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCa                                        >  4:393684/6‑137 (MQ=255)
                                                                                            cttcttCCCCTCTTCTGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCATAg                                     >  1:78455/5‑139 (MQ=255)
                                                                                                    cctcttctGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCATAGCTTTTCGa                             <  5:329064/137‑1 (MQ=255)
                                                                                                      tcttctGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCATAGCTTTTCGa                             >  2:18381/1‑137 (MQ=255)
                                                                                                          ctGACTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCATAGCTTTTCGAGAGc                         <  8:216032/137‑1 (MQ=255)
                                                                                                             aCTTCCCGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATAATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCATAGCTTTTCGAGAGCGGc                      <  7:389148/137‑1 (MQ=255)
                                                                                                                  ccGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGc                                                                 >  8:2450/1‑89 (MQ=255)
                                                                                                                  ccGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCATAGCTTTTCTCGAGCGGCAtata                 >  3:404668/1‑137 (MQ=255)
                                                                                                                  ccGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCATAGCTTTTCGAGAGc                         >  7:198118/1‑129 (MQ=255)
                                                                                                                  ccGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCATAGCTTTTCGAGAGc                         <  8:198118/129‑1 (MQ=255)
                                                                                                                  ccGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCATAGCTTTTCGAGAGCGGCAtata                 >  5:111698/1‑137 (MQ=255)
                                                                                                                  ccGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCATAGCTTTTCGAGAGCGGCAtata                 >  4:214087/1‑137 (MQ=255)
                                                                                                                  ccGATTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCATAGCTTTTCGAGAGCGGCAtata                 >  2:31296/1‑137 (MQ=255)
                                                                                                                     aTTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCATAGCTTTTCGAGAGCGGCATATAAAGTg            <  1:335661/139‑1 (MQ=255)
                                                                                                                     aTTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCATAGCTTTTCGAGAGCGGCATATAAAGTg            <  2:169791/139‑1 (MQ=255)
                                                                                                                     aTTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCATAGCTTTTCGAGAGCGGCATATAAAGTg            <  1:335675/139‑1 (MQ=255)
                                                                                                                     aTTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCATAGCTTTTCGAGAGCGGCATATAAAGTg            <  6:333025/139‑1 (MQ=255)
                                                                                                                     aTTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCATAGCTTTTCGAGAGCGGCATATAAAGTg            <  3:371226/139‑1 (MQ=255)
                                                                                                                     aTTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCATAGCTTTTCGAGAGCGGCATATAAAGTg            <  3:393684/139‑1 (MQ=255)
                                                                                                                     aTTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCATAGCTTTTCGAGAGCGGCATATAAAGTg            <  7:419186/139‑1 (MQ=255)
                                                                                                                     aTTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCATAGCTTTTCGAGAGCGGCATATAAAGTg            <  4:118934/139‑1 (MQ=255)
                                                                                                                     aTTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCATAGCTTTTCGAGAGCGGCATATAAAGTg            <  4:75359/139‑1 (MQ=255)
                                                                                                                     aTTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCATAGCTTTTCGAGAGCGGCATATAAAGTg            <  5:138193/139‑1 (MQ=255)
                                                                                                                     aTTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCATAGCTTTTCGAGAGCGGCATATAAAGTg            <  5:200098/139‑1 (MQ=255)
                                                                                                                     aTTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCATAGCTTTTCGAGAGCGGCATATAAAGTg            <  6:432590/139‑1 (MQ=255)
                                                                                                                     aTTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCATAGCTTTTCGAGAGCGGCATATAAAGTg            <  5:343936/139‑1 (MQ=255)
                                                                                                                     aTTTTCAAGGCCATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCATAGCTTTTCGAGAGCGGCATATAAAGTg            <  6:200035/139‑1 (MQ=255)
                                                                                                                               ccATTTTGGCGGCATAAAGGAAAAGTTCTTTGTTATATTCATCATATCTGATCCCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATTCCTTCAGCTTCATAGCTTTTCGAGAGCGGCATATAAAGTGAAGTATAAGa  <  1:4699/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                   
TGTTCATAATCCTCTTCTTTATGATACACGGCAAGAAGTGTATGAAGTGCCTCGACGAAGCCCGGATCAAGTGCAAGCGCCTCCTGAAGCAGCTGTTTCCCATCTTCTGACTTCCCGATTTTTAAAGCCATTTTTGCGGCATAGAGGAAAAGCTCTTTGTTATATTCATCATATCTGATACCCTCTTTTGCCGTTTTTAATGCCTCTTCATACATCCCTTCAGCTTCATAGCTTTTCGAAAGCGGCATATAAAGTGAAGAATAAGA  >  CP006881/2184495‑2184760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑