Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,172,830 A→C L306L (CTT→CTG)  U712_10870 ← Bifunctional protein BirA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,172,8300AC98.9% 296.5 / NA 91L306L (CTT→CTG) U712_10870Bifunctional protein BirA
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (39/51);  total (39/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTATAATAGGTGCAGTTCGCAGGCGATACTGCCCAATGGAAGTATACCAAAATCAACGGGCTTATACCAACACATTAACCCAATTCAATATCGGCAGAATAGATTTTTTTAATGCCTTCCTGCGTTTCTAAAAGAAGAACGCCTTCATCATCTATACCTAACGCCTTCCCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCGATTCCCAACGCATAACTTTCCCATAAAAGCTTAATCGGCGTAAAACCGTGTG  >  CP006881/2172695‑2172960
                                                                                                                                       |                                                                                                                                  
ttaataataGGTGCAGTTCGCAGGCGATACTGCCCAATGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAaagcag                                                                                                                                  >  6:7687/4‑138 (MQ=255)
ttaataataGGTGCAGTTCGCAGGCGATACTGCCCAATGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAaagcag                                                                                                                                  >  6:46398/4‑138 (MQ=255)
ttaataataGGTGCAGTTCGCAGGCGATACTGCCCAATGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAagcaga                                                                                                                                 >  5:327899/4‑139 (MQ=255)
 taataataGGTGCAGTTCGCAGGCGATACTGCCCAATGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAagcaga                                                                                                                                 <  7:327136/136‑1 (MQ=255)
 taataataGGTGCAGTTCGCAGGCGATACTGCCCAATGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAagcaga                                                                                                                                 <  2:344800/136‑1 (MQ=255)
 taataataGGTGCAGTTCGCAGGCGATACTGCCCAATGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAagcaga                                                                                                                                 <  8:68532/136‑1 (MQ=255)
  aattataGGTGCAGTTCGCAGGCGATACTGACCAATGGAAGTATACCAAAATCAAAGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAgcagaa                                                                                                                                <  3:278703/134‑1 (MQ=255)
     aataGGTGCAGTTCGCAGGCGATACTGCCCAATGGAAGTATACCAAACTCCACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGcc                                                                                                                            <  8:348065/139‑1 (MQ=255)
        aGGTGCAGTTCGCAGGCGATACTGCCCAATGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCtt                                                                                                                          >  2:258321/1‑138 (MQ=255)
         ggTGCAGTTCGCAGGCGATACTGCCCAATGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTtc                                                                                                                         <  6:148821/138‑1 (MQ=255)
         ggTGCAGTTCGCAGGCGATACTGCCCAATGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTtc                                                                                                                         <  8:348032/138‑1 (MQ=255)
         ggTGCAGTTCGCAGGCGATACTGCCCAATGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTtc                                                                                                                         <  6:416625/138‑1 (MQ=255)
          gTGCAGTTCGCAGGCGATACTGCCCAATGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTtcat                                                                                                                       <  5:295181/139‑1 (MQ=255)
          gTGCAGTTCGCAGGCGATACTGCCCAATGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTtcat                                                                                                                       <  5:7687/139‑1 (MQ=255)
          gTGCAGTTCGCAGGCGATACTGCCCAATGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTtcat                                                                                                                       <  6:107475/139‑1 (MQ=255)
             cAGTTCGCAGGCGATACTGCCCAATGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTcatcat                                                                                                                    <  7:128845/139‑1 (MQ=255)
                ttCGCAGGCGATACTGCCCAATGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCta                                                                                                                 <  2:442336/139‑1 (MQ=255)
                     aGGCGATACTGCCCAATGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATAcc                                                                                                             >  6:199225/1‑138 (MQ=255)
                     aGGCGATACTGCCCAATGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATAcc                                                                                                             >  1:224885/1‑138 (MQ=255)
                     aGGCGATACTGCCCAATGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATAcc                                                                                                             >  4:212416/1‑138 (MQ=255)
                      ggCGATACTGCCCAATGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTa                                                                                                           <  6:54665/139‑1 (MQ=255)
                          aTACTGCCCAATGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGc                                                                                                       >  5:401223/1‑139 (MQ=255)
                                 ccAATGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCg                                                                                                >  4:300328/1‑139 (MQ=255)
                                   aaTGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGt                                                                                               >  6:184384/1‑138 (MQ=255)
                                     tGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTtca                                                                                                                        >  4:193578/1‑111 (MQ=255)
                                     tGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTtca                                                                                                                        <  3:193578/111‑1 (MQ=255)
                                     tGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTaa                                                                                             >  2:133899/1‑138 (MQ=255)
                                     tGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTaa                                                                                             >  3:381133/1‑138 (MQ=255)
                                     tGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTaa                                                                                             >  4:366947/1‑138 (MQ=255)
                                     tGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTaa                                                                                             >  1:46896/1‑138 (MQ=255)
                                     tGGAAGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTaa                                                                                             >  1:116984/1‑138 (MQ=255)
                                         aGTATACCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGt                                                                                        >  1:261282/1‑139 (MQ=255)
                                             taCCAAAATCAACGGGCTTGTACCAACACATTAGCCGAATTCGATATCGGCAGAATAGATTTTTTTTATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTACCGCCTTACCGTAAAAGGTTCCg                                                                                    >  1:70715/1‑139 (MQ=255)
                                             taCCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCg                                                                                    >  6:415100/1‑139 (MQ=255)
                                             taCCAAAATCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCg                                                                                    >  1:80615/1‑139 (MQ=255)
                                                  aaaTCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTaa                                                                               >  6:435752/1‑139 (MQ=255)
                                                  aaaTCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTaa                                                                               <  8:258908/139‑1 (MQ=255)
                                                  aaaTCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTaa                                                                               >  8:31948/1‑139 (MQ=255)
                                                   aaTCAACGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGACTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAAc                                                                              >  4:362792/1‑139 (MQ=255)
                                                         cGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTg                                                                        >  5:85116/1‑139 (MQ=255)
                                                         cGGGCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTg                                                                        <  3:300328/139‑1 (MQ=255)
                                                           ggCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGc                                                                      <  8:11247/139‑1 (MQ=255)
                                                           ggCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGc                                                                      <  6:85116/139‑1 (MQ=255)
                                                           ggCTTGTACCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGCTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGc                                                                      <  3:277883/139‑1 (MQ=255)
                                                                gtaCCAACACATTAGCCCAATTCGATCTCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGctct                                                                   <  2:133228/136‑1 (MQ=255)
                                                                gtaCCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGctct                                                                   <  8:365520/136‑1 (MQ=255)
                                                                 taCCAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCa                                                                 >  3:345951/1‑138 (MQ=255)
                                                                    cAACACATTATCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATAtt                                                             <  6:23366/139‑1 (MQ=255)
                                                                    cAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATAtt                                                             <  4:381133/139‑1 (MQ=255)
                                                                    cAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATAtt                                                             <  6:6514/139‑1 (MQ=255)
                                                                    cAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATAtt                                                             <  5:46398/139‑1 (MQ=255)
                                                                    cAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATAtt                                                             <  3:418560/139‑1 (MQ=255)
                                                                    cAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATAtt                                                             <  5:435752/139‑1 (MQ=255)
                                                                    cAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATAtt                                                             <  2:69177/139‑1 (MQ=255)
                                                                    cAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATAtt                                                             <  3:299093/139‑1 (MQ=255)
                                                                    cAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATAtt                                                             <  1:258321/139‑1 (MQ=255)
                                                                    cAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATAtt                                                             <  3:366947/139‑1 (MQ=255)
                                                                    cAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATAtt                                                             <  4:388515/139‑1 (MQ=255)
                                                                    cAACACATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGGTCCGTTTAACGTTCTGGCTCTCATAtt                                                             <  2:86384/139‑1 (MQ=255)
                                                                      acacATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTtc                                                                                                                         <  5:106402/77‑1 (MQ=255)
                                                                      acacATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTtc                                                                                                                         >  6:106402/1‑77 (MQ=255)
                                                                      acacATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCt                                                                                                     >  5:436746/1‑97 (MQ=255)
                                                                      acacATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAg                                                           >  3:426717/1‑139 (MQ=255)
                                                                      acacATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAg                                                           <  7:31948/139‑1 (MQ=255)
                                                                      acacATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACATAACGCCt                                                                                                     <  6:436746/97‑1 (MQ=255)
                                                                      acacATTAGCCCAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGAAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAg                                                           <  4:326242/139‑1 (MQ=255)
                                                                               cccAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTtca                                                                                                                        >  2:147804/1‑69 (MQ=255)
                                                                               cccAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTtca                                                                                                                        <  1:147804/69‑1 (MQ=255)
                                                                               cccAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAAtac                                                  >  2:32325/1‑137 (MQ=255)
                                                                                ccAATTCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATAcc                                                 <  6:327899/139‑1 (MQ=255)
                                                                                    ttCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGtt                                                                                       <  7:381808/97‑1 (MQ=255)
                                                                                    ttCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGtt                                                                                       >  8:381808/1‑97 (MQ=255)
                                                                                    ttCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTgg                                                                       <  8:206004/113‑1 (MQ=255)
                                                                                    ttCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTgg                                                                       >  7:206004/1‑113 (MQ=255)
                                                                                    ttCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATAtt                                                             <  6:287163/123‑1 (MQ=255)
                                                                                    ttCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATAtt                                                             >  5:287163/1‑123 (MQ=255)
                                                                                    ttCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATAtt                                                             <  4:81323/123‑1 (MQ=255)
                                                                                    ttCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATAtt                                                             >  3:81323/1‑123 (MQ=255)
                                                                                    ttCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATAccgagc                                             >  2:285347/1‑135 (MQ=255)
                                                                                    ttCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATAccgagc                                             >  1:365564/1‑135 (MQ=255)
                                                                                    ttCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATAccgagc                                             >  1:332920/1‑135 (MQ=255)
                                                                                    ttCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATAccgagc                                             >  7:94125/1‑135 (MQ=255)
                                                                                    ttCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATAcc                                                 >  3:11944/1‑135 (MQ=255)
                                                                                    ttCGATATCGGCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATAcc                                                 <  4:11944/135‑1 (MQ=255)
                                                                                              gCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCttt                                   >  1:247455/1‑139 (MQ=255)
                                                                                                    tAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATa                             <  8:94125/139‑1 (MQ=255)
                                                                                                    tAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATa                             <  6:401223/139‑1 (MQ=255)
                                                                                                    tAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATa                             <  5:184384/139‑1 (MQ=255)
                                                                                                    tAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATa                             <  1:133899/139‑1 (MQ=255)
                                                                                                       aTTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCt                                                                                                     <  7:180347/64‑1 (MQ=255)
                                                                                                       aTTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCt                                                                                                     >  8:180347/1‑64 (MQ=255)
                                                                                                               aaTGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAAt                   <  4:426717/138‑1 (MQ=255)
                                                                                                               aaTGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAAt                   <  4:345951/138‑1 (MQ=255)
                                                                                                               aaTGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAAt                   <  1:285347/138‑1 (MQ=255)
                                                                                                                             tttCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAAAACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCgtg    >  5:25980/1‑139 (MQ=255)
                                                                                                                               tCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCgtgc   <  2:247455/138‑2 (MQ=255)
                                                                                                                                cTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCgtgcg  <  6:25980/138‑3 (MQ=255)
                                                                                                                                cTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCgtgcg  <  3:362792/138‑3 (MQ=255)
                                                                                                                                       |                                                                                                                                  
TTTATAATAGGTGCAGTTCGCAGGCGATACTGCCCAATGGAAGTATACCAAAATCAACGGGCTTATACCAACACATTAACCCAATTCAATATCGGCAGAATAGATTTTTTTAATGCCTTCCTGCGTTTCTAAAAGAAGAACGCCTTCATCATCTATACCTAACGCCTTCCCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCGATTCCCAACGCATAACTTTCCCATAAAAGCTTAATCGGCGTAAAACCGTGTG  >  CP006881/2172695‑2172960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑