Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,185,016 A→G A150A (GCT→GCC)  U712_10945 ← TPR repeat‑containing protein ypiA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,185,0160AG96.2% 242.5 / NA 80A150A (GCT→GCC) U712_10945TPR repeat‑containing protein ypiA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (32/45);  minor base T (2/1);  total (34/46)
Fisher's exact test for biased strand distribution p-value = 5.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACCACGGAATGGCATCCTCAAATTCACCTGAGGCACTTAACGATTCGGCCAGTCTTTGATGGACATTAACGCCTCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAAGCTCCCTGAGTAAAGTAAAGTTCCCCCAGTGCAAAATCAATAATGGGTTCATTGTCCAGAATGGACTTTGCTTGTAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGGTAAAGATCCGCCATAAGC  >  CP006881/2184885‑2185151
                                                                                                                                   |                                                                                                                                       
tACCATGGAATGGCATCCTCAAATTCACCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCt                                                                                                                                  <  5:381688/139‑1 (MQ=255)
   catggAATGGCATCCTCAAATTCACCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGag                                                                                                                               <  7:360586/136‑1 (MQ=255)
   catggAATGGCATCCTCAAATTCACCTGAGGCACTTAACGATTCTGCCAGTATTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGag                                                                                                                               <  2:383988/136‑1 (MQ=255)
     tggAATGGCATCCTCAAATTCACCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGATGTTTTTAAATATTTAACCGCTTTTGCATGGGCGCACTGagg                                                                                                                              >  1:322315/2‑137 (MQ=255)
     tggAATGGCATCCTCAAATTCACCTGAGGCACTTAACGATTCTGCCAGTCTTTGATCGACATTAACGCCCCCGATCTCACTATGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGagc                                                                                                                              >  8:35294/2‑137 (MQ=255)
       gAATGGCATCCTCAAATTCACCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGagcaa                                                                                                                            >  4:89200/1‑138 (MQ=255)
         aTGGCATCCTCAAATTCACCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGagcaaagt                                                                                                                         <  6:271266/139‑1 (MQ=255)
             cATCCTCAAATTCACCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTTAAATATTGAACCGCTTTTGCATAGGCTCCCGGagcaaagtaacg                                                                                                                     >  5:10987/1‑137 (MQ=255)
             cATCCTCAAATTCACCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGcaaagtaaag                                                                                                                     >  5:109523/1‑139 (MQ=255)
             cATCCTCAAATTCACCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGcaaagtaaag                                                                                                                     >  6:318129/1‑139 (MQ=255)
                  tCAAATTCACCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGag                                                                                                                               >  8:327510/1‑124 (MQ=255)
                  tCAAATTCACCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGag                                                                                                                               <  7:327510/124‑1 (MQ=255)
                  tCAAATTCACCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTctc                                                                                                                >  4:63904/1‑137 (MQ=255)
                  tCAAATTCACCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTctc                                                                                                                >  1:42406/1‑137 (MQ=255)
                  tCAAATTCACCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTctc                                                                                                                >  2:53605/1‑137 (MQ=255)
                    aaaTTCACCTGAGGAACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAATATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTctcc                                                                                                               <  3:323868/138‑1 (MQ=255)
                      aTTCACCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAg                                                                                                            >  2:426321/1‑139 (MQ=255)
                          aCCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCa                                                                                                        <  3:89200/139‑1 (MQ=255)
                          aCCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCa                                                                                                        <  3:172027/139‑1 (MQ=255)
                          aCCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCa                                                                                                        <  6:7658/139‑1 (MQ=255)
                          aCCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCa                                                                                                        <  5:306522/139‑1 (MQ=255)
                          aCCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCa                                                                                                        <  7:129497/139‑1 (MQ=255)
                          aCCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCa                                                                                                        <  7:185208/139‑1 (MQ=255)
                          aCCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCa                                                                                                        <  7:283833/139‑1 (MQ=255)
                          aCCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCa                                                                                                        <  1:426321/139‑1 (MQ=255)
                               aGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCACAGCCGTTGGTTTAAAATATTGAACAGCATTTGCATATGCTCCCTGAGCAAAGAAAAGTTCTCCCAGTGCAAAAt                                                                                                    >  3:284971/1‑138 (MQ=255)
                                ggCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCa                                                                                                  <  4:32309/139‑1 (MQ=255)
                                    cTTAACGTTTCTGACAGTCTTTGATGTACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCACTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCaataa                                                                                              <  2:370843/139‑1 (MQ=255)
                                    cTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCaataa                                                                                              >  8:186965/1‑139 (MQ=255)
                                      tAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCaataaca                                                                                            >  8:435227/1‑137 (MQ=255)
                                      tAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCaataa                                                                                              >  3:448800/1‑137 (MQ=255)
                                         cGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCaataacaggt                                                                                         >  6:413042/1‑134 (MQ=255)
                                            ttCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCa                                                                                      <  3:147962/139‑1 (MQ=255)
                                            ttCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCa                                                                                      <  3:400809/139‑1 (MQ=255)
                                            ttCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCa                                                                                      <  7:186965/139‑1 (MQ=255)
                                                 ccAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTc                                                                                 <  5:318129/139‑1 (MQ=255)
                                                     tCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGa                                                                             <  2:42406/139‑1 (MQ=255)
                                                         tGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGaa                                                                            <  6:109523/136‑1 (MQ=255)
                                                                        cccccGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATATGCTCCCTGAGCAAAGTAAAGTTCTCACAGTGCAAAATCAATAAAAGGTTCATTTTCCAGAATGGACTTTGCCTGGaaaa                                                          >  7:86811/4‑139 (MQ=255)
                                                                         ccccGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCaat                                                                                                >  6:321587/3‑100 (MQ=255)
                                                                         ccccGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCaat                                                                                                <  5:321587/98‑1 (MQ=255)
                                                                         ccccGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAg                                                         >  3:211510/3‑139 (MQ=255)
                                                                          cccGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGc                                                        >  2:220820/2‑139 (MQ=255)
                                                                                   agtctGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGACCAAAGTAAAGTTCTCCCAGTGCAAAATCAATATCAGGTTCATTGTCCAGATTGGACTTTTCCGGTATCAGCTTTAGTTCa                                               >  4:270210/3‑139 (MQ=255)
                                                                                   aCTCTGCTCCTCAGCCGTTGTTTTAAAATCTTGAACCGCTTTTGCCTAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCttt                                                     <  2:347689/133‑1 (MQ=255)
                                                                                   aCTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCtt                                                      <  4:448800/132‑1 (MQ=255)
                                                                                   aCTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTg                                                   <  6:165222/135‑1 (MQ=255)
                                                                                   aCTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGttga                                               >  6:135783/1‑137 (MQ=255)
                                                                                   aCTCTGCTCCTCAGCCGTTGGTTTAAAATATTGAACCGCTTTTGCAAGGGCTCCCAGTGCAAACTTAAGTTCACCCAGGGCACAATGAATTACAGGTGCATTGGCCAGCTTGGACTTTGCCTGGAATAGGGTTTGttga                                               >  2:437522/1‑137 (MQ=255)
                                                                                    ctctGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCAc                                              <  7:35294/139‑1 (MQ=255)
                                                                                     gctGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACt                                             <  2:316495/138‑1 (MQ=255)
                                                                                       tGCTCCTCAGCCGTTGGTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACt                                             <  5:15500/137‑1 (MQ=255)
                                                                                        gCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGaaa                                                           <  6:296349/122‑1 (MQ=255)
                                                                                        gCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGaaa                                                           >  5:296349/1‑122 (MQ=255)
                                                                                         ctcctcATCCGGTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGAc                                          <  5:90855/138‑1 (MQ=255)
                                                                                         ctcctcAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACt                                             >  8:274125/1‑135 (MQ=255)
                                                                                         ctcctcAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACt                                             <  7:274125/135‑1 (MQ=255)
                                                                                         ctcctcAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGAc                                          <  7:435227/138‑1 (MQ=255)
                                                                                         ctcctcAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACt                                         >  4:405193/1‑139 (MQ=255)
                                                                                         ctcctcAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGGAAAGTTCTCCCAGTGCAAAATAAATAACAGGTTCATTGTCCAGAATGGCCTTTGCCTGGAAAAGCTTTTGGTCCCTGACt                                         >  3:267441/1‑139 (MQ=255)
                                                                                               aggcGTTGTTTTATAATATTGAACCGCTTTTGCATAGGCTCCCTTATCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCATAATTGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAAt                                   <  1:54389/136‑1 (MQ=255)
                                                                                               aGCCGTTTTTTTAAAATATTGAACCGCTTTTCCATAGGCTCCCTGAGCAAAGTAAAGTTCTCACAGTCCAAAATCAATAACAGGTTCATTTTCCAAATTTGCCTTTGCCTTGAAAAGATTTTGTTAACTGACTTAGaa                                    >  6:429250/1‑138 (MQ=255)
                                                                                               aGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCa                                                                                      <  4:157074/88‑1 (MQ=255)
                                                                                               aGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCa                                                                                      >  3:157074/1‑88 (MQ=255)
                                                                                                   gttgttTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCaataa                                                                                              >  5:67206/1‑76 (MQ=255)
                                                                                                   gttgttTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCaataa                                                                                              <  6:67206/76‑1 (MQ=255)
                                                                                                         ttAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGc                          <  1:53605/138‑1 (MQ=255)
                                                                                                            aaaTAGTGAACCGCTTTTGCATAGGCTCCATGAGCAAAGTAAAGTTCACCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATc                       <  2:322315/138‑1 (MQ=255)
                                                                                                                 ttGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATa                  >  3:150930/1‑138 (MQ=255)
                                                                                                                 ttGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATa                  >  5:422229/1‑138 (MQ=255)
                                                                                                                     aCCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGAGTTCGAATAACCCCTGCATCTGATAAAGAt             <  4:323238/139‑1 (MQ=255)
                                                                                                                     aCCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGAt             <  4:147891/139‑1 (MQ=255)
                                                                                                                     aCCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGAt             <  1:220820/139‑1 (MQ=255)
                                                                                                                     aCCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAAAAACAGGTTCATTGTCCAGTATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACATCGAATAACCCCTGCATCTGATAAAGAt             <  5:429250/139‑1 (MQ=255)
                                                                                                                      ccGCTTTTGCATATGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATc            <  5:376796/139‑1 (MQ=255)
                                                                                                                         cTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCaataa                                                                                              <  7:18/54‑1 (MQ=255)
                                                                                                                         cTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCaataa                                                                                              >  8:18/1‑54 (MQ=255)
                                                                                                                         cTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGc         >  7:284282/1‑139 (MQ=255)
                                                                                                                             tGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCAt      <  2:90988/138‑1 (MQ=255)
                                                                                                                                aTAGGCTCCCTGAGCAAAGTAAAGTTCTCCCATTGAAAAATCAATAAAATGTTCATTGTCCAGAATGGACTTTGCCTTGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGAATCTGATAAAGATCCGCCATAagc  <  2:14782/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                       
TACCACGGAATGGCATCCTCAAATTCACCTGAGGCACTTAACGATTCGGCCAGTCTTTGATGGACATTAACGCCTCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAAGCTCCCTGAGTAAAGTAAAGTTCCCCCAGTGCAAAATCAATAATGGGTTCATTGTCCAGAATGGACTTTGCTTGTAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGGTAAAGATCCGCCATAAGC  >  CP006881/2184885‑2185151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑