Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,185,027	T→C	T147A (ACT→GCT)	U712_10945 ←	TPR repeat‑containing protein ypiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,185,027	0	T	C	100.0%	268.5 / NA	82	T147A (ACT→GCT)	U712_10945	TPR repeat‑containing protein ypiA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/44);  total (38/44)

GAATGGCATCCTCAAATTCACCTGAGGCACTTAACGATTCGGCCAGTCTTTGATGGACATTAACGCCTCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAAGCTCCCTGAGTAAAGTAAAGTTCCCCCAGTGCAAAATCAATAATGGGTTCATTGTCCAGAATGGACTTTGCTTGTAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGGTAAAGATCCGCCATAAGCAGCAAACT  >  CP006881/2184892‑2185159                                                                                                                                        |                                                                                                                                     gAATGGCATCCTCAAATTCACCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGagcaa                                                                                                                                    >  4:89200/1‑138 (MQ=255)   aTGGCATCCTCAAATTCACCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGagcaaagt                                                                                                                                 <  6:271266/139‑1 (MQ=255)       cATCCTCAAATTCACCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTTAAATATTGAACCGCTTTTGCATAGGCTCCCGGagcaaagtaacg                                                                                                                             >  5:10987/1‑137 (MQ=255)       cATCCTCAAATTCACCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGcaaagtaaag                                                                                                                             >  6:318129/1‑139 (MQ=255)       cATCCTCAAATTCACCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGcaaagtaaag                                                                                                                             >  5:109523/1‑139 (MQ=255)            tCAAATTCACCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTctc                                                                                                                        >  2:53605/1‑137 (MQ=255)            tCAAATTCACCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTctc                                                                                                                        >  1:42406/1‑137 (MQ=255)            tCAAATTCACCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTctc                                                                                                                        >  4:63904/1‑137 (MQ=255)              aaaTTCACCTGAGGAACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAATATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTctcc                                                                                                                       <  3:323868/138‑1 (MQ=255)                aTTCACCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAg                                                                                                                    >  2:426321/1‑139 (MQ=255)                    aCCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCa                                                                                                                <  3:172027/139‑1 (MQ=255)                    aCCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCa                                                                                                                <  3:89200/139‑1 (MQ=255)                    aCCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCa                                                                                                                <  5:306522/139‑1 (MQ=255)                    aCCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCa                                                                                                                <  6:7658/139‑1 (MQ=255)                    aCCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCa                                                                                                                <  7:129497/139‑1 (MQ=255)                    aCCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCa                                                                                                                <  1:426321/139‑1 (MQ=255)                    aCCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCa                                                                                                                <  7:185208/139‑1 (MQ=255)                    aCCTGAGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCa                                                                                                                <  7:283833/139‑1 (MQ=255)                         aGGCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCACAGCCGTTGGTTTAAAATATTGAACAGCATTTGCATATGCTCCCTGAGCAAAGAAAAGTTCTCCCAGTGCAAAAt                                                                                                            >  3:284971/1‑138 (MQ=255)                          ggCACTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCa                                                                                                          <  4:32309/139‑1 (MQ=255)                              cTTAACGTTTCTGACAGTCTTTGATGTACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCACTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCaataa                                                                                                      <  2:370843/139‑1 (MQ=255)                              cTTAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCaataa                                                                                                      >  8:186965/1‑139 (MQ=255)                                tAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCaataaca                                                                                                    >  8:435227/1‑137 (MQ=255)                                tAACGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCaataa                                                                                                      >  3:448800/1‑137 (MQ=255)                                   cGATTCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCaataacaggt                                                                                                 >  6:413042/1‑134 (MQ=255)                                      ttCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCa                                                                                              <  3:147962/139‑1 (MQ=255)                                      ttCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCa                                                                                              <  3:400809/139‑1 (MQ=255)                                      ttCTGCCAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCa                                                                                              <  7:186965/139‑1 (MQ=255)                                           ccAGTCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTc                                                                                         <  5:318129/139‑1 (MQ=255)                                               tCTTTGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGa                                                                                     <  2:42406/139‑1 (MQ=255)                                                   tGATGGACATTAACGCCCCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGaa                                                                                    <  6:109523/136‑1 (MQ=255)                                                                  cccccGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATATGCTCCCTGAGCAAAGTAAAGTTCTCACAGTGCAAAATCAATAAAAGGTTCATTTTCCAGAATGGACTTTGCCTGGaaaa                                                                  >  7:86811/4‑139 (MQ=255)                                                                   ccccGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCaat                                                                                                        >  6:321587/3‑100 (MQ=255)                                                                   ccccGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCaat                                                                                                        <  5:321587/98‑1 (MQ=255)                                                                   ccccGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAg                                                                 >  3:211510/3‑139 (MQ=255)                                                                    cccGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGc                                                                >  2:220820/2‑139 (MQ=255)                                                                             agtctGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGACCAAAGTAAAGTTCTCCCAGTGCAAAATCAATATCAGGTTCATTGTCCAGATTGGACTTTTCCGGTATCAGCTTTAGTTCa                                                       >  4:270210/3‑139 (MQ=255)                                                                             aCTCTGCTCCTCAGCCGTTGTTTTAAAATCTTGAACCGCTTTTGCCTAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCttt                                                             <  2:347689/133‑1 (MQ=255)                                                                             aCTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCtt                                                              <  4:448800/132‑1 (MQ=255)                                                                             aCTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTg                                                           <  6:165222/135‑1 (MQ=255)                                                                             aCTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGttga                                                       >  6:135783/1‑137 (MQ=255)                                                                             aCTCTGCTCCTCAGCCGTTGGTTTAAAATATTGAACCGCTTTTGCAAGGGCTCCCAGTGCAAACTTAAGTTCACCCAGGGCACAATGAATTACAGGTGCATTGGCCAGCTTGGACTTTGCCTGGAATAGGGTTTGttga                                                       >  2:437522/1‑137 (MQ=255)                                                                              ctctGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCAc                                                      <  7:35294/139‑1 (MQ=255)                                                                               gctGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACt                                                     <  2:316495/138‑1 (MQ=255)                                                                                 tGCTCCTCAGCCGTTGGTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACt                                                     <  5:15500/137‑1 (MQ=255)                                                                                  gCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGaaa                                                                   >  5:296349/1‑122 (MQ=255)                                                                                  gCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGaaa                                                                   <  6:296349/122‑1 (MQ=255)                                                                                   ctcctcATCCGGTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGAc                                                  <  5:90855/138‑1 (MQ=255)                                                                                   ctcctcAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACt                                                     >  8:274125/1‑135 (MQ=255)                                                                                   ctcctcAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACt                                                     <  7:274125/135‑1 (MQ=255)                                                                                   ctcctcAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGAc                                                  <  7:435227/138‑1 (MQ=255)                                                                                   ctcctcAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACt                                                 >  4:405193/1‑139 (MQ=255)                                                                                   ctcctcAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGGAAAGTTCTCCCAGTGCAAAATAAATAACAGGTTCATTGTCCAGAATGGCCTTTGCCTGGAAAAGCTTTTGGTCCCTGACt                                                 >  3:267441/1‑139 (MQ=255)                                                                                         aggcGTTGTTTTATAATATTGAACCGCTTTTGCATAGGCTCCCTTATCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCATAATTGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAAt                                           <  1:54389/136‑1 (MQ=255)                                                                                         aGCCGTTTTTTTAAAATATTGAACCGCTTTTCCATAGGCTCCCTGAGCAAAGTAAAGTTCTCACAGTCCAAAATCAATAACAGGTTCATTTTCCAAATTTGCCTTTGCCTTGAAAAGATTTTGTTAACTGACTTAGaa                                            >  6:429250/1‑138 (MQ=255)                                                                                         aGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCa                                                                                              <  4:157074/88‑1 (MQ=255)                                                                                         aGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCa                                                                                              >  3:157074/1‑88 (MQ=255)                                                                                             gttgttTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCaataa                                                                                                      >  5:67206/1‑76 (MQ=255)                                                                                             gttgttTTAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCaataa                                                                                                      <  6:67206/76‑1 (MQ=255)                                                                                                   ttAAAATATTGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGc                                  <  1:53605/138‑1 (MQ=255)                                                                                                      aaaTAGTGAACCGCTTTTGCATAGGCTCCATGAGCAAAGTAAAGTTCACCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATc                               <  2:322315/138‑1 (MQ=255)                                                                                                           ttGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATa                          >  5:422229/1‑138 (MQ=255)                                                                                                           ttGAACCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATa                          >  3:150930/1‑138 (MQ=255)                                                                                                               aCCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGAGTTCGAATAACCCCTGCATCTGATAAAGAt                     <  4:323238/139‑1 (MQ=255)                                                                                                               aCCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGAt                     <  1:220820/139‑1 (MQ=255)                                                                                                               aCCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGAt                     <  4:147891/139‑1 (MQ=255)                                                                                                               aCCGCTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAAAAACAGGTTCATTGTCCAGTATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACATCGAATAACCCCTGCATCTGATAAAGAt                     <  5:429250/139‑1 (MQ=255)                                                                                                                ccGCTTTTGCATATGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATc                    <  5:376796/139‑1 (MQ=255)                                                                                                                   cTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCaataa                                                                                                      >  8:18/1‑54 (MQ=255)                                                                                                                   cTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCaataa                                                                                                      <  7:18/54‑1 (MQ=255)                                                                                                                   cTTTTGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGc                 >  7:284282/1‑139 (MQ=255)                                                                                                                       tGCATAGGCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCAt              <  2:90988/138‑1 (MQ=255)                                                                                                                          aTAGGCTCCCTGAGCAAAGTAAAGTTCTCCCATTGAAAAATCAATAAAATGTTCATTGTCCAGAATGGACTTTGCCTTGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGAATCTGATAAAGATCCGCCATAagc          <  2:14782/139‑1 (MQ=255)                                                                                                                             ggCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGAc                                                  <  6:153931/95‑1 (MQ=255)                                                                                                                             ggCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGAc                                                  >  5:153931/2‑96 (MQ=255)                                                                                                                              gCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCAtt                                                                                            >  5:297340/1‑53 (MQ=255)                                                                                                                              gCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCAtt                                                                                            <  6:297340/53‑1 (MQ=255)                                                                                                                              gCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAagcagc       >  1:354666/1‑138 (MQ=255)                                                                                                                              gCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAagcagc       >  1:385043/1‑138 (MQ=255)                                                                                                                              gCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAagcagc       >  2:196321/1‑138 (MQ=255)                                                                                                                              gCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAagcagc       >  3:143910/1‑138 (MQ=255)                                                                                                                              gCTCCCTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAgcagca      <  1:247270/139‑1 (MQ=255)                                                                                                                                 cccTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTGTGCCTGGAAAAGAGTTTGTTCACTGACTTCGAATACACCCTGCATCTGATAAAGATCAGCCATAAGAAGCAAAc   >  5:65413/1‑139 (MQ=255)                                                                                                                                  ccTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACt  >  5:223793/1‑139 (MQ=255)                                                                                                                                  ccTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACt  >  1:426452/1‑139 (MQ=255)                                                                                                                                  ccTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAATAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACt  >  2:30840/1‑139 (MQ=255)                                                                                                                                  ccTGAGCAAAGTAAAGTTCTCCCAGTGCAAAATCAAAAACAGGTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGATCACTGACTTCGCATAACCCCAGCATCTGATAAAGATCCGCCATAAGCAGCAAACt  >  6:71859/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                     GAATGGCATCCTCAAATTCACCTGAGGCACTTAACGATTCGGCCAGTCTTTGATGGACATTAACGCCTCCGATCTCACTCTGCTCCTCAGCCGTTGTTTTAAAATATTGAACCGCTTTTGCATAAGCTCCCTGAGTAAAGTAAAGTTCCCCCAGTGCAAAATCAATAATGGGTTCATTGTCCAGAATGGACTTTGCTTGTAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGGTAAAGATCCGCCATAAGCAGCAAACT  >  CP006881/2184892‑2185159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑