Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,185,190 G→A T92T (ACC→ACT)  U712_10945 ← TPR repeat‑containing protein ypiA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,185,1900GA98.8% 257.1 / NA 82T92T (ACC→ACT) U712_10945TPR repeat‑containing protein ypiA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (36/45);  minor base T (0/1);  total (36/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAATGGGTTCATTGTCCAGAATGGACTTTGCTTGTAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGGTAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTATCCGGAATGGTTTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAAAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAAATCACTGATTAGTGAAATCGCTTTTTCTACAT  >  CP006881/2185057‑2185323
                                                                                                                                     |                                                                                                                                     
taacaggTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGtctc                                                                                                                                  <  2:26162/134‑1 (MQ=255)
      gTTCATTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGca                                                                                                                             >  4:372482/1‑138 (MQ=255)
          aTTGTCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCg                                                                                                                        <  2:141862/139‑1 (MQ=255)
              tCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAggg                                                                                                                    >  3:434345/1‑139 (MQ=255)
              tCCAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTAGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAgg                                                                                                                     <  6:223793/138‑1 (MQ=255)
                cAGAATGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCt                                                                                                                  >  4:394321/1‑139 (MQ=255)
                   aaTGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCtttt                                                                                                               >  7:196873/1‑139 (MQ=255)
                   aaTGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCtttt                                                                                                               >  7:175004/1‑139 (MQ=255)
                   aaTGGACTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCtttt                                                                                                               >  7:174999/1‑139 (MQ=255)
                        aCTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTttcttc                                                                                                          >  4:300003/1‑139 (MQ=255)
                        aCTTTGCCGGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATCAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTttcttc                                                                                                          <  4:267441/139‑1 (MQ=255)
                         cTTTGCCTGGAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTttcttct                                                                                                         <  6:10987/139‑1 (MQ=255)
                             gcctggaaaaGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGt                                                                                                     >  8:69280/7‑139 (MQ=255)
                             gcctggaaaaGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGt                                                                                                     >  6:88523/7‑139 (MQ=255)
                             gcctggaaaaGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGt                                                                                                     >  4:36139/7‑139 (MQ=255)
                             gcctggaaaaGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGt                                                                                                     >  3:434456/7‑139 (MQ=255)
                                  gaaaaGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAata                                                                                                <  8:196873/138‑1 (MQ=255)
                                   aaaaGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAatat                                                                                               <  1:196321/139‑1 (MQ=255)
                                   aaaaGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAatat                                                                                               <  1:74682/139‑1 (MQ=255)
                                   aaaaGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAatat                                                                                               <  3:63904/139‑1 (MQ=255)
                                                cACTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGtt                                                                                   <  5:135783/138‑1 (MQ=255)
                                                cACTGACTTCGAATAACCCCAGCATCTGATCAAGATCCGCCATCAGCAGCAAACTTTCCGGATATGCTGGATCTGTTTCCGTAATAGTCTCCAGCACAGCGAGGTCTTTTTCTTCTTCGTCAATATCTATTAAAAGtt                                                                                   <  3:270210/138‑1 (MQ=255)
                                                  cTGACTTCGAATAACCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAg                                                                                >  3:115172/1‑139 (MQ=255)
                                                               aCCCCTGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGt                                                                   >  7:441220/1‑139 (MQ=255)
                                                                    tGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGtt                                                              >  1:410547/1‑139 (MQ=255)
                                                                    tGCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGtt                                                              >  2:386249/1‑139 (MQ=255)
                                                                     gCATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGAGGGATCTGTTTCCGGAAGAGTCTCCCGCACAGCGAGGGCTTTTTCTTCTTCGTCACTATCTAGTAAAAGTTCAGCGTAGAAATTCGTCTGTTc                                                             >  4:273721/1‑139 (MQ=255)
                                                                      cATCTGATAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCt                                                            >  3:10404/1‑139 (MQ=255)
                                                                          tgatAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGt                                                          <  4:284552/134‑1 (MQ=255)
                                                                           gatAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAat                                                                                                 <  4:228053/95‑1 (MQ=255)
                                                                           gatAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAat                                                                                                 >  3:228053/3‑97 (MQ=255)
                                                                           gatAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGtt                                                                                   <  7:289371/109‑1 (MQ=255)
                                                                           gatAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGtt                                                                                   >  8:289371/3‑111 (MQ=255)
                                                                           gatAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGt                                                                   >  7:411978/3‑127 (MQ=255)
                                                                           gatAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGt                                                                   <  8:411978/125‑1 (MQ=255)
                                                                           gatAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGttt                                                        >  4:382741/3‑138 (MQ=255)
                                                                           gatAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGttt                                                        >  3:298004/3‑138 (MQ=255)
                                                                           gatAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGttt                                                        >  3:219918/3‑138 (MQ=255)
                                                                               aaGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCAttt                                                   <  2:354666/139‑1 (MQ=255)
                                                                               aaGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCAtt                                                    <  5:88523/138‑1 (MQ=255)
                                                                               aaGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCAtt                                                    <  7:2461/138‑1 (MQ=255)
                                                                               aaGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCAtt                                                    <  3:303288/138‑1 (MQ=255)
                                                                               aaGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCAtt                                                    <  3:36139/138‑1 (MQ=255)
                                                                                  aTCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGa                                                <  2:385043/139‑1 (MQ=255)
                                                                                  aTCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTAATAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGa                                                <  4:143910/139‑1 (MQ=255)
                                                                                   tCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGat                                               <  2:125401/139‑1 (MQ=255)
                                                                                    ccGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGata                                              >  7:291935/1‑139 (MQ=255)
                                                                                    ccGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTACGGAATAGTCTCCAGCACAGCGAGGGCTTTttcttct                                                                                                         >  2:234981/1‑80 (MQ=255)
                                                                                    ccGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTACGGAATAGTCTCCAGCACAGCGAGGGCTTTttcttct                                                                                                         <  1:234981/80‑1 (MQ=255)
                                                                                     cGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGACAGTTCTGTTTCATTTGGata                                              >  5:287601/1‑138 (MQ=255)
                                                                                      gCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGatat                                             >  8:232366/1‑138 (MQ=255)
                                                                                       ccATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATaa                                           <  8:291935/139‑1 (MQ=255)
                                                                                        cATAAGCAGCAAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGACAGTTCTGTTTCATTTGGATATaaa                                          <  6:287601/139‑1 (MQ=255)
                                                                                               agcaAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTttcttc                                                                                                          <  5:324208/68‑1 (MQ=255)
                                                                                               agcaAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTttcttc                                                                                                          >  6:324208/1‑68 (MQ=255)
                                                                                               agcaAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTa                                   <  8:441220/139‑1 (MQ=255)
                                                                                               agcaAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTa                                   <  2:426452/139‑1 (MQ=255)
                                                                                               agcaAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTa                                   <  4:314101/139‑1 (MQ=255)
                                                                                                 caAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTagaaa                                                                        >  8:48447/1‑100 (MQ=255)
                                                                                                 caAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTagaaa                                                                        <  7:48447/100‑1 (MQ=255)
                                                                                                 caAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATg                                     >  1:126281/1‑135 (MQ=255)
                                                                                                 caAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATg                                     >  5:322112/1‑135 (MQ=255)
                                                                                                 caAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATg                                     >  5:439850/1‑135 (MQ=255)
                                                                                                 caAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATg                                     >  5:446153/1‑135 (MQ=255)
                                                                                                 caAACTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATg                                     >  1:424378/1‑135 (MQ=255)
                                                                                                  aaaCTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGAt                                <  3:273721/139‑1 (MQ=255)
                                                                                                  aaaCTTTCAGGATATGATTGAGCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTGTTTCTTCTTCGTCAATATCTATTAAAAGTTCATCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGAt                                <  6:65413/139‑1 (MQ=255)
                                                                                                    aCTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCa                              <  4:298004/139‑1 (MQ=255)
                                                                                                    aCTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCa                              <  4:219918/139‑1 (MQ=255)
                                                                                                    aCTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCa                              <  4:115172/139‑1 (MQ=255)
                                                                                                        tCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACta                           <  8:174999/138‑2 (MQ=255)
                                                                                                        tCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACta                           <  1:30840/138‑2 (MQ=255)
                                                                                                        tCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCAGGTAGATCACta                           <  8:175004/138‑2 (MQ=255)
                                                                                                                tgatgGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGa                   >  4:179743/1‑138 (MQ=255)
                                                                                                                   tgGATCTGTTTCCGGAATAGTCACCAGCACAGCGAGGGCCTTTTCTTCTTCCTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTAGGATATAAATCATGTAGATCACTAATCAGTGAAAtg               <  8:168017/139‑2 (MQ=255)
                                                                                                                     gATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGc             <  6:439850/139‑1 (MQ=255)
                                                                                                                     gATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGc             <  6:446153/139‑1 (MQ=255)
                                                                                                                     gATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGc             <  4:434456/139‑1 (MQ=255)
                                                                                                                      aTCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCt            >  1:54844/1‑139 (MQ=255)
                                                                                                                               cGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACa   <  4:211510/139‑1 (MQ=255)
                                                                                                                                ggAATTGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACAt  <  6:422229/139‑1 (MQ=255)
                                                                                                                                ggAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACAt  <  8:175814/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                     
TAATGGGTTCATTGTCCAGAATGGACTTTGCTTGTAAAAGCTTTTGTTCACTGACTTCGAATAACCCCTGCATCTGGTAAAGATCCGCCATAAGCAGCAAACTTTCCGGATATGATGGATCTGTATCCGGAATGGTTTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAAAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAAATCACTGATTAGTGAAATCGCTTTTTCTACAT  >  CP006881/2185057‑2185323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑