Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,185,291	A→G	L59L (TTA→CTA)	U712_10945 ←	TPR repeat‑containing protein ypiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,185,291	0	A	G	100.0%	300.5 / NA	91	L59L (TTA→CTA)	U712_10945	TPR repeat‑containing protein ypiA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/56);  total (35/56)

AAACTTTCCGGATATGATGGATCTGTATCCGGAATGGTTTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAAAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAAATCACTGATTAGTGAAATCGCTTTTTCTACATTGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTGTCTTCATCATAGAGCTGATTTTCAGCTTTCGAGAGAGTGTTGAGGCCTTTTTCTGTTTCACCTGC  >  CP006881/2185155‑2185426                                                                                                                                         |                                                                                                                                        aaaCTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGAt                                                                                                                                       <  3:273721/139‑1 (MQ=255) aaaCTTTCAGGATATGATTGAGCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTGTTTCTTCTTCGTCAATATCTATTAAAAGTTCATCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGAt                                                                                                                                       <  6:65413/139‑1 (MQ=255)   aCTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCa                                                                                                                                     <  4:115172/139‑1 (MQ=255)   aCTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCa                                                                                                                                     <  4:219918/139‑1 (MQ=255)   aCTTTCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCa                                                                                                                                     <  4:298004/139‑1 (MQ=255)       tCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACta                                                                                                                                  <  1:30840/138‑2 (MQ=255)       tCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACta                                                                                                                                  <  8:174999/138‑2 (MQ=255)       tCCGGATATGATGGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCAGGTAGATCACta                                                                                                                                  <  8:175004/138‑2 (MQ=255)               tgatgGATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGa                                                                                                                          >  4:179743/1‑138 (MQ=255)                  tgGATCTGTTTCCGGAATAGTCACCAGCACAGCGAGGGCCTTTTCTTCTTCCTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTAGGATATAAATCATGTAGATCACTAATCAGTGAAAtg                                                                                                                      <  8:168017/139‑2 (MQ=255)                    gATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGc                                                                                                                    <  6:439850/139‑1 (MQ=255)                    gATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGc                                                                                                                    <  6:446153/139‑1 (MQ=255)                    gATCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGc                                                                                                                    <  4:434456/139‑1 (MQ=255)                     aTCTGTTTCCGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCt                                                                                                                   >  1:54844/1‑139 (MQ=255)                              cGGAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACa                                                                                                          <  4:211510/139‑1 (MQ=255)                               ggAATTGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACAt                                                                                                         <  6:422229/139‑1 (MQ=255)                               ggAATAGTCTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACAt                                                                                                         <  8:175814/139‑1 (MQ=255)                                    agtctCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGccc                                                                                                    >  4:139910/5‑139 (MQ=255)                                     gtctCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGccc                                                                                                    <  4:10404/135‑1 (MQ=255)                                          cAGGACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCTGTTCTGTTTGATTTGGTTATTAATCATGTAGATCACTAATCACTGAAATGGCTTTATCTACATCGCCCCCCt                                                                                                <  1:437522/137‑1 (MQ=255)                                          cAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACt                                                                                                <  3:382741/137‑1 (MQ=255)                                          cAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACt                                                                                                <  6:322112/137‑1 (MQ=255)                                          cAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACt                                                                                                <  7:69280/137‑1 (MQ=255)                                               caGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGATATGGCTTTTTCTACATCGCCCCACTCGtaat                                                                                          >  1:373559/1‑138 (MQ=255)                                                aGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGTATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGtaata                                                                                         >  8:332189/1‑138 (MQ=255)                                                aGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCa                                                                                                                                     >  5:396347/1‑94 (MQ=255)                                                aGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCa                                                                                                                                     <  6:396347/94‑1 (MQ=255)                                                aGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACa                                                                                                          <  3:357343/121‑1 (MQ=255)                                                aGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACt                                                                                                <  5:245209/131‑1 (MQ=255)                                                aGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACt                                                                                                >  6:245209/1‑131 (MQ=255)                                                aGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGtaata                                                                                         >  7:168063/1‑138 (MQ=255)                                                aGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATAACTAATCAGTGAAATGGCTTTTTCTACa                                                                                                          >  4:357343/1‑121 (MQ=255)                                                aGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTAATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGtaata                                                                                         >  7:158866/1‑138 (MQ=255)                                                  cGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGcc                                                                                                     <  6:220150/124‑1 (MQ=255)                                                  cGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGcc                                                                                                     >  5:220150/1‑124 (MQ=255)                                                  cGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAg                                                                                      >  3:150258/1‑139 (MQ=255)                                                  cGAGGGCTTGTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAg                                                                                      >  7:243480/1‑139 (MQ=255)                                                    aGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCt                                                                                    >  6:380148/1‑139 (MQ=255)                                                    aGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTGTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTGTTCTACCTCGCCCCACTCGTAATAAAGCt                                                                                    >  4:279535/1‑139 (MQ=255)                                                       gCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGGGTAGTAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCTCTCGTAATAAAGCTggg                                                                                 <  5:71859/139‑1 (MQ=255)                                                       gCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTggg                                                                                 >  4:186039/1‑139 (MQ=255)                                                       gCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTggg                                                                                 >  6:406157/1‑139 (MQ=255)                                                       gCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTggg                                                                                 <  6:211733/139‑1 (MQ=255)                                                       gCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTggg                                                                                 <  3:179743/139‑1 (MQ=255)                                                       gCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTggg                                                                                 <  7:232366/139‑1 (MQ=255)                                                       gCTTTTTCTTCTTCGTCAATATCTATTAAAAGGTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTggg                                                                                 <  8:445210/139‑1 (MQ=255)                                                             tcttcttcGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCa                                                                            <  3:300003/138‑1 (MQ=255)                                                             tcttcttcGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCa                                                                            <  1:386249/138‑1 (MQ=255)                                                             tcttcttcGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCa                                                                            <  2:410547/138‑1 (MQ=255)                                                             tcttcttcGTCAATATCTAATAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCa                                                                            <  2:443518/138‑1 (MQ=255)                                                              cttcttcGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCaa                                                                           <  3:372482/138‑1 (MQ=255)                                                                 cttcGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACt                                                                                                <  1:69785/114‑1 (MQ=255)                                                                 cttcGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACt                                                                                                >  2:69785/1‑114 (MQ=255)                                                                 cttcGTCAATATCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGc                                                                       >  2:428535/1‑139 (MQ=255)                                                                    agTCAATATCTATTAAAAGTTCATCGTAGAAATTCTTCAGTTATGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATAGCCACACTCGTAATAAAGCTGGGCTGAAATGGc                                                                       <  4:101338/135‑1 (MQ=255)                                                                            tCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTtca                                                             <  5:380148/138‑1 (MQ=255)                                                                                ttAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTcatcatg                                                        >  8:57451/1‑138 (MQ=255)                                                                                   aaaGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTcatcatgcag                                                     <  3:394321/139‑5 (MQ=255)                                                                                     aGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCt                                                   <  4:150258/139‑1 (MQ=255)                                                                                      gTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTg                                                  >  7:314170/1‑139 (MQ=255)                                                                                        tCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTgtt                                                >  8:46101/1‑137 (MQ=255)                                                                                         cAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGttt                                               >  2:51770/1‑139 (MQ=255)                                                                                          aGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGtttt                                              >  1:80328/1‑139 (MQ=255)                                                                                           gCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTtttt                                             <  5:413042/139‑1 (MQ=255)                                                                                           gCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTtttt                                             <  8:243480/139‑1 (MQ=255)                                                                                           gCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTtttt                                             <  8:168063/139‑1 (MQ=255)                                                                                           gCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTtttt                                             <  7:57451/139‑1 (MQ=255)                                                                                           gCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGACCCACTCTTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTtttt                                             <  1:364426/139‑1 (MQ=255)                                                                                             gTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCa                                           <  7:46101/139‑1 (MQ=255)                                                                                                      cGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCgag                                  >  7:353850/1‑139 (MQ=255)                                                                                                       ggaatttCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCgaga                                 <  2:54844/134‑1 (MQ=255)                                                                                                        tCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCgagag                                <  5:406157/139‑1 (MQ=255)                                                                                                        tCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCgagag                                <  3:186039/139‑1 (MQ=255)                                                                                                            ttCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGATTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCgtg                            >  5:254381/1‑139 (MQ=255)                                                                                                               tGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACTTCGCCCCAGTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTg                         <  7:378193/139‑1 (MQ=255)                                                                                                                 tttCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTg                                                                                   >  7:106473/1‑79 (MQ=255)                                                                                                                 tttCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTg                                                                                   <  8:106473/79‑1 (MQ=255)                                                                                                                 tttCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTg                         <  4:434345/137‑1 (MQ=255)                                                                                                                      tttGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCaa                                                                           >  7:213216/1‑82 (MQ=255)                                                                                                                      tttGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCaa                                                                           <  8:213216/82‑1 (MQ=255)                                                                                                                      tttGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTGTTCAGCCTTCGAGAGCGTGTTGAGGCCtt                  >  3:55546/1‑139 (MQ=255)                                                                                                                                 aTCCTGTAGAGCACTACTCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGGTGAGGCCTTTTTCTGTTTCa       <  3:279535/139‑1 (MQ=255)                                                                                                                                     tGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCaa                                                                           <  3:216334/67‑1 (MQ=255)                                                                                                                                     tGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCaa                                                                           >  4:216334/1‑67 (MQ=255)                                                                                                                                     tGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCaa                                                                           >  8:413625/1‑67 (MQ=255)                                                                                                                                     tGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCaa                                                                           <  7:413625/67‑1 (MQ=255)                                                                                                                                     tGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTg   <  3:220330/139‑1 (MQ=255)                                                                                                                                     tGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTg   >  4:220330/1‑139 (MQ=255)                                                                                                                                     tGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTg   >  1:67285/1‑139 (MQ=255)                                                                                                                                     tGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTg   >  1:62833/1‑139 (MQ=255)                                                                                                                                      gTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGc  >  7:376345/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                        AAACTTTCCGGATATGATGGATCTGTATCCGGAATGGTTTCCAGCACAGCGAGGGCTTTTTCTTCTTCGTCAATATCTATTAAAAGTTCAGCGTAAAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAAATCACTGATTAGTGAAATCGCTTTTTCTACATTGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTGTCTTCATCATAGAGCTGATTTTCAGCTTTCGAGAGAGTGTTGAGGCCTTTTTCTGTTTCACCTGC  >  CP006881/2185155‑2185426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑