Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,185,361	G→A	D35D (GAC→GAT)	U712_10945 ←	TPR repeat‑containing protein ypiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,185,361	0	G	A	100.0%	275.5 / NA	88	D35D (GAC→GAT)	U712_10945	TPR repeat‑containing protein ypiA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (47/41);  total (47/41)

TCTATTAAAAGTTCAGCGTAAAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAAATCACTGATTAGTGAAATCGCTTTTTCTACATTGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTGTCTTCATCATAGAGCTGATTTTCAGCTTTCGAGAGAGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCCTTTCTTTTCCTATA  >  CP006881/2185230‑2185492                                                                                                                                    |                                                                                                                                    tCTATTAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTtca                                                                                                                               <  5:380148/138‑1 (MQ=255)     ttAAAAGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTcatcatg                                                                                                                          >  8:57451/1‑138 (MQ=255)        aaaGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTcatcatgcag                                                                                                                       <  3:394321/139‑5 (MQ=255)          aGTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCt                                                                                                                     <  4:150258/139‑1 (MQ=255)           gTTCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTg                                                                                                                    >  7:314170/1‑139 (MQ=255)             tCAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTgtt                                                                                                                  >  8:46101/1‑137 (MQ=255)              cAGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGttt                                                                                                                 >  2:51770/1‑139 (MQ=255)               aGCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGtttt                                                                                                                >  1:80328/1‑139 (MQ=255)                gCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTtttt                                                                                                               <  5:413042/139‑1 (MQ=255)                gCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTtttt                                                                                                               <  8:243480/139‑1 (MQ=255)                gCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTtttt                                                                                                               <  8:168063/139‑1 (MQ=255)                gCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTtttt                                                                                                               <  7:57451/139‑1 (MQ=255)                gCGTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGACCCACTCTTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTtttt                                                                                                               <  1:364426/139‑1 (MQ=255)                  gTAGAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCa                                                                                                             <  7:46101/139‑1 (MQ=255)                           cGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCgag                                                                                                    >  7:353850/1‑139 (MQ=255)                            ggaatttCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCgaga                                                                                                   <  2:54844/134‑1 (MQ=255)                             tCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCgagag                                                                                                  <  5:406157/139‑1 (MQ=255)                             tCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCgagag                                                                                                  <  3:186039/139‑1 (MQ=255)                                 ttCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGATTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCgtg                                                                                              >  5:254381/1‑139 (MQ=255)                                    tGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACTTCGCCCCAGTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTg                                                                                           <  7:378193/139‑1 (MQ=255)                                      tttCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTg                                                                                           <  4:434345/137‑1 (MQ=255)                                           tttGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTGTTCAGCCTTCGAGAGCGTGTTGAGGCCtt                                                                                    >  3:55546/1‑139 (MQ=255)                                                      aTCCTGTAGAGCACTACTCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGGTGAGGCCTTTTTCTGTTTCa                                                                         <  3:279535/139‑1 (MQ=255)                                                          tGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTg                                                                     >  4:220330/1‑139 (MQ=255)                                                          tGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTg                                                                     >  1:67285/1‑139 (MQ=255)                                                          tGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTg                                                                     >  1:62833/1‑139 (MQ=255)                                                          tGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTg                                                                     <  3:220330/139‑1 (MQ=255)                                                           gTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGc                                                                    >  7:376345/1‑139 (MQ=255)                                                               aTCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTc                                                                 >  8:415598/1‑138 (MQ=255)                                                                   ctaatcaGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACGTGCTTCAACt                                                             >  1:424431/7‑138 (MQ=255)                                                                    taatcaGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAAc                                                              <  5:234688/131‑1 (MQ=255)                                                                    taatcaGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAAc                                                              >  6:234688/6‑136 (MQ=255)                                                                    taatcaGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTATTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTaa                                                           >  7:351041/6‑139 (MQ=255)                                                                      atcaGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAAtt                                                         >  5:283670/4‑139 (MQ=255)                                                                        caGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAAttt                                                        >  3:182214/2‑138 (MQ=255)                                                                         aGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTa                                                      >  6:288715/1‑139 (MQ=255)                                                                           tGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCTATGGCTTTATCTTCATCATGCAGCTGTTTTTCCGCCTTCGAGAGCGTGTTGAGGAGTTTTTCTGTTTCACCTGCTACAACTAATTTTATg                                                    >  5:205525/1‑139 (MQ=255)                                                                           tGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCt                                                                                                                                  >  1:51297/1‑61 (MQ=255)                                                                           tGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCt                                                                                                                                  <  2:51297/61‑1 (MQ=255)                                                                           tGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCa                                                                                                             >  5:25945/1‑82 (MQ=255)                                                                           tGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCa                                                                                                             <  6:19923/82‑1 (MQ=255)                                                                           tGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCa                                                                                                             <  6:25945/82‑1 (MQ=255)                                                                           tGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCa                                                                                                             <  4:87976/82‑1 (MQ=255)                                                                           tGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCa                                                                                                             >  3:87976/1‑82 (MQ=255)                                                                           tGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCa                                                                                                             >  5:19923/1‑82 (MQ=255)                                                                           tGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATg                                                    >  1:391744/1‑139 (MQ=255)                                                                           tGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACATGCTTCAACTAATTTTATg                                                    >  2:201872/1‑139 (MQ=255)                                                                           tGAAATGGCTTTTTCTACATCGACCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATg                                                    >  1:233094/1‑139 (MQ=255)                                                                             aaaTGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATgg                                                   <  6:283670/138‑1 (MQ=255)                                                                                     ttttCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCgaga                                                                                                   <  2:66944/82‑1 (MQ=255)                                                                                     ttttCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCgaga                                                                                                   >  1:66944/1‑82 (MQ=255)                                                                                     ttttCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGaa                                          >  4:180491/1‑139 (MQ=255)                                                                                     ttttCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGaa                                          >  4:104405/1‑139 (MQ=255)                                                                                       ttCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAAt                                         <  2:80328/138‑1 (MQ=255)                                                                                          tACATCGCACCACTCGTAATAAAGCTTGTCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCTAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAAt                                     <  3:345673/139‑1 (MQ=255)                                                                                            cctcgCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAAt                                     <  2:373559/133‑1 (MQ=255)                                                                                            cATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATg                                    >  5:230876/1‑138 (MQ=255)                                                                                            cATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCATTTTTCTGTTTCACCTGCTTCAAGTAATTTTATTGATTCTTGAATCAATGt                                   >  4:345673/1‑139 (MQ=255)                                                                                            cATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTCTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAAt                                     <  4:55546/137‑1 (MQ=255)                                                                                                gCCACACTCGTAATTAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTAtt                                <  7:332189/138‑1 (MQ=255)                                                                                                         gTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCATTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGa                       <  6:254381/138‑1 (MQ=255)                                                                                                           aataaAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCg                    >  6:404054/1‑139 (MQ=255)                                                                                                              aaaGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTc                 >  8:445635/1‑139 (MQ=255)                                                                                                                aGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATg                                                    >  1:203476/1‑102 (MQ=255)                                                                                                                aGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATg                                                    <  2:203476/102‑1 (MQ=255)                                                                                                                aGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTAtt                                >  3:237903/1‑122 (MQ=255)                                                                                                                aGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTAtt                                <  4:237903/122‑1 (MQ=255)                                                                                                                aGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCTAATCGGACCGGTCct               >  1:157621/1‑139 (MQ=255)                                                                                                                aGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCct               >  6:202564/1‑139 (MQ=255)                                                                                                                 gCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCTCCTAATTTTATGGCTGCTTGAATCAATGTATTCAAATCGGACCGGGccgt              >  1:443483/1‑137 (MQ=255)                                                                                                                   tGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAAt                           <  3:406434/124‑1 (MQ=255)                                                                                                                   tGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAAt                           >  4:406434/1‑124 (MQ=255)                                                                                                                    gggCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCctttct           >  1:9433/1‑139 (MQ=255)                                                                                                                     ggCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCt                                                                   <  7:237685/82‑1 (MQ=255)                                                                                                                     ggCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCt                                                                   >  8:237685/1‑82 (MQ=255)                                                                                                                     ggCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGAc                      <  1:204000/127‑1 (MQ=255)                                                                                                                     ggCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGAc                      >  2:204000/1‑127 (MQ=255)                                                                                                                     ggCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCctttctt          >  8:53351/1‑139 (MQ=255)                                                                                                                     ggCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCctttctt          >  4:422668/1‑139 (MQ=255)                                                                                                                     ggCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCctttctt          >  3:370077/1‑139 (MQ=255)                                                                                                                     ggCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCctttctt          >  6:250384/1‑139 (MQ=255)                                                                                                                     ggCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCctttc            <  2:126281/137‑1 (MQ=255)                                                                                                                          cAATGGCTTTATCTTCCTCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCCTTTCTTTTc       <  1:428535/137‑1 (MQ=255)                                                                                                                          cAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCCTTTCTTTTc       <  7:415598/137‑1 (MQ=255)                                                                                                                             tGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCCTTTCTTTTCCtata  <  2:391744/139‑1 (MQ=255)                                                                                                                             tGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCCTTTCTTTTCCtata  <  8:376345/139‑1 (MQ=255)                                                                                                                             tGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCCTTTCTTTTCCtata  <  2:424378/139‑1 (MQ=255)                                                                                                                             tGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCCTTTCTTTTCCtata  <  3:139910/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                    TCTATTAAAAGTTCAGCGTAAAAATTCGTCAGTTCTGTTTCATTTGGATATAAATCATGTAAATCACTGATTAGTGAAATCGCTTTTTCTACATTGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTGTCTTCATCATAGAGCTGATTTTCAGCTTTCGAGAGAGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCCTTTCTTTTCCTATA  >  CP006881/2185230‑2185492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑