Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,185,388	T→C	K26K (AAA→AAG)	U712_10945 ←	TPR repeat‑containing protein ypiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,185,388	0	T	C	100.0%	269.1 / NA	83	K26K (AAA→AAG)	U712_10945	TPR repeat‑containing protein ypiA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (48/35);  total (48/35)

CGTCAGTTCTGTTTCATTTGGATATAAATCATGTAAATCACTGATTAGTGAAATCGCTTTTTCTACATTGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTGTCTTCATCATAGAGCTGATTTTCAGCTTTCGAGAGAGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCCTTTCTTTTCCTATAGAACATCAACCATAC‑AAAAAAGCTTTATTT  >  CP006881/2185256‑2185522                                                                                                                                     |                                                                                                                                        cGTCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCgag                                                                                                                                   >  7:353850/1‑139 (MQ=255)  ggaatttCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCgaga                                                                                                                                  <  2:54844/134‑1 (MQ=255)   tCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCgagag                                                                                                                                 <  3:186039/139‑1 (MQ=255)   tCAGTTCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCgagag                                                                                                                                 <  5:406157/139‑1 (MQ=255)       ttCTGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGATTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCgtg                                                                                                                             >  5:254381/1‑139 (MQ=255)          tGTTTCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACTTCGCCCCAGTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTg                                                                                                                          <  7:378193/139‑1 (MQ=255)            tttCATTTGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTg                                                                                                                          <  4:434345/137‑1 (MQ=255)                 tttGGATATAAATCATGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTGTTCAGCCTTCGAGAGCGTGTTGAGGCCtt                                                                                                                   >  3:55546/1‑139 (MQ=255)                            aTCCTGTAGAGCACTACTCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGGTGAGGCCTTTTTCTGTTTCa                                                                                                        <  3:279535/139‑1 (MQ=255)                                tGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTg                                                                                                    >  4:220330/1‑139 (MQ=255)                                tGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTg                                                                                                    <  3:220330/139‑1 (MQ=255)                                tGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTg                                                                                                    >  1:67285/1‑139 (MQ=255)                                tGTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTg                                                                                                    >  1:62833/1‑139 (MQ=255)                                 gTAGATCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGc                                                                                                   >  7:376345/1‑139 (MQ=255)                                     aTCACTAATCAGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTc                                                                                                >  8:415598/1‑138 (MQ=255)                                         ctaatcaGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACGTGCTTCAACt                                                                                            >  1:424431/7‑138 (MQ=255)                                          taatcaGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAAc                                                                                             >  6:234688/6‑136 (MQ=255)                                          taatcaGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAAc                                                                                             <  5:234688/131‑1 (MQ=255)                                          taatcaGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTATTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTaa                                                                                          >  7:351041/6‑139 (MQ=255)                                            atcaGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAAtt                                                                                        >  5:283670/4‑139 (MQ=255)                                              caGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAAttt                                                                                       >  3:182214/2‑138 (MQ=255)                                               aGTGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTa                                                                                     >  6:288715/1‑139 (MQ=255)                                                 tGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCTATGGCTTTATCTTCATCATGCAGCTGTTTTTCCGCCTTCGAGAGCGTGTTGAGGAGTTTTTCTGTTTCACCTGCTACAACTAATTTTATg                                                                                   >  5:205525/1‑139 (MQ=255)                                                 tGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATg                                                                                   >  1:391744/1‑139 (MQ=255)                                                 tGAAATGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACATGCTTCAACTAATTTTATg                                                                                   >  2:201872/1‑139 (MQ=255)                                                 tGAAATGGCTTTTTCTACATCGACCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATg                                                                                   >  1:233094/1‑139 (MQ=255)                                                   aaaTGGCTTTTTCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATgg                                                                                  <  6:283670/138‑1 (MQ=255)                                                           ttttCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCgaga                                                                                                                                  <  2:66944/82‑1 (MQ=255)                                                           ttttCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCgaga                                                                                                                                  >  1:66944/1‑82 (MQ=255)                                                           ttttCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGaa                                                                         >  4:180491/1‑139 (MQ=255)                                                           ttttCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGaa                                                                         >  4:104405/1‑139 (MQ=255)                                                             ttCTACATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAAt                                                                        <  2:80328/138‑1 (MQ=255)                                                                tACATCGCACCACTCGTAATAAAGCTTGTCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCTAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAAt                                                                    <  3:345673/139‑1 (MQ=255)                                                                  cctcgCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAAt                                                                    <  2:373559/133‑1 (MQ=255)                                                                  cATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATg                                                                   >  5:230876/1‑138 (MQ=255)                                                                  cATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCATTTTTCTGTTTCACCTGCTTCAAGTAATTTTATTGATTCTTGAATCAATGt                                                                  >  4:345673/1‑139 (MQ=255)                                                                  cATCGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTCTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAAt                                                                    <  4:55546/137‑1 (MQ=255)                                                                      gCCACACTCGTAATTAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTAtt                                                               <  7:332189/138‑1 (MQ=255)                                                                               gTAATAAAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCATTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGa                                                      <  6:254381/138‑1 (MQ=255)                                                                                 aataaAGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCg                                                   >  6:404054/1‑139 (MQ=255)                                                                                    aaaGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTc                                                >  8:445635/1‑139 (MQ=255)                                                                                      aGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATg                                                                                   >  1:203476/1‑102 (MQ=255)                                                                                      aGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATg                                                                                   <  2:203476/102‑1 (MQ=255)                                                                                      aGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTAtt                                                               <  4:237903/122‑1 (MQ=255)                                                                                      aGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTAtt                                                               >  3:237903/1‑122 (MQ=255)                                                                                      aGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCTAATCGGACCGGTCct                                              >  1:157621/1‑139 (MQ=255)                                                                                      aGCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCct                                              >  6:202564/1‑139 (MQ=255)                                                                                       gCTGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCTCCTAATTTTATGGCTGCTTGAATCAATGTATTCAAATCGGACCGGGccgt                                             >  1:443483/1‑137 (MQ=255)                                                                                         tGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAAt                                                          <  3:406434/124‑1 (MQ=255)                                                                                         tGGGCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAAt                                                          >  4:406434/1‑124 (MQ=255)                                                                                          gggCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCctttct                                          >  1:9433/1‑139 (MQ=255)                                                                                           ggCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCt                                                                                                  >  8:237685/1‑82 (MQ=255)                                                                                           ggCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCt                                                                                                  <  7:237685/82‑1 (MQ=255)                                                                                           ggCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGAc                                                     <  1:204000/127‑1 (MQ=255)                                                                                           ggCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGAc                                                     >  2:204000/1‑127 (MQ=255)                                                                                           ggCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCctttctt                                         >  4:422668/1‑139 (MQ=255)                                                                                           ggCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCctttctt                                         >  3:370077/1‑139 (MQ=255)                                                                                           ggCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCctttctt                                         >  6:250384/1‑139 (MQ=255)                                                                                           ggCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCctttctt                                         >  8:53351/1‑139 (MQ=255)                                                                                           ggCTGCAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCctttc                                           <  2:126281/137‑1 (MQ=255)                                                                                                cAATGGCTTTATCTTCCTCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCCTTTCTTTTc                                      <  1:428535/137‑1 (MQ=255)                                                                                                cAATGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCCTTTCTTTTc                                      <  7:415598/137‑1 (MQ=255)                                                                                                   tGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCCTTTCTTTTCCtata                                 <  2:391744/139‑1 (MQ=255)                                                                                                   tGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCCTTTCTTTTCCtata                                 <  2:424378/139‑1 (MQ=255)                                                                                                   tGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCCTTTCTTTTCCtata                                 <  3:139910/139‑1 (MQ=255)                                                                                                   tGGCTTTATCTTCATCATGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCCTTTCTTTTCCtata                                 <  8:376345/139‑1 (MQ=255)                                                                                                               catcatGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCCTTTCTTTTCCTATACAACATCAAcc                      >  6:333859/1‑138 (MQ=255)                                                                                                               catcatGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCCTTTCTTTTCCTATACAACATCAAcc                      <  5:404054/138‑1 (MQ=255)                                                                                                               catcatGCAGCAGTTTTTCAGCCTTAGAGATCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGAACCGGACCTTTATTTTCATATACAACATAAAca                      >  7:21701/1‑137 (MQ=255)                                                                                                                atcatGCAGCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCCTTTCTTTTCCTATACAACATCAACCa                     >  4:126338/1‑138 (MQ=255)                                                                                                                     gcagCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCTGCCCGGTCCTTTCTTTTCCTATACAACATCAACCATAC‑aa               >  3:422368/3‑138 (MQ=255)                                                                                                                     gcagCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCCTTTCTTTTCCTATACAACATCAACCATAC‑aa               >  7:419203/3‑138 (MQ=255)                                                                                                                      cagCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCCTGTCTTTTCCTATACAACATCAACCATAC‑aaaa             <  3:126338/138‑1 (MQ=255)                                                                                                                      cagCTGTTTTTCAGCCTTCGAGAGCGTGTGGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCCTTTCTTTTCCTATACAACATCAACCATAC‑aaa              <  8:351041/137‑1 (MQ=255)                                                                                                                       agCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCctttc                                           >  4:20143/1‑109 (MQ=255)                                                                                                                       agCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCctttc                                           <  3:20143/109‑1 (MQ=255)                                                                                                                       agCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGGCCTTTCTTTTCCTATACAACATCAACCATAC‑aaaaa            <  5:202564/139‑1 (MQ=255)                                                                                                                        gCTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCCTTTCTTTTCCTATACAACATCAACCATAC‑aaaaaa           <  2:233094/139‑1 (MQ=255)                                                                                                                         cTGTTTTTCAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCCTTTCTTTTCCTATACAACATCAACCATAC‑aaaaa            >  7:25456/1‑137 (MQ=255)                                                                                                                              tttCAGCCTTCGAGAGCGTGTTGCGGCCTTTTTCTGTTTCACCTGCGTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCCTTTCTTTTCCTATACAACATCAACCATACAAAAAAAGCttt      >  8:7676/1‑139 (MQ=255)                                                                                                                                 cAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAAt                                                                    <  4:272043/74‑1 (MQ=255)                                                                                                                                 cAGCCTTCGAGAGCGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAAt                                                                    >  3:272043/1‑74 (MQ=255)                                                                                                                                  aGCCTTCGAGAGCGTGTTGAGGCCTATTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCCTTTCTTTTCCTATACAACATCAACCATACAAAAAAAGCTTTAttt  >  3:315515/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                        CGTCAGTTCTGTTTCATTTGGATATAAATCATGTAAATCACTGATTAGTGAAATCGCTTTTTCTACATTGCCCCACTCGTAATAAAGCTGGGCTGCAATGGCTTTGTCTTCATCATAGAGCTGATTTTCAGCTTTCGAGAGAGTGTTGAGGCCTTTTTCTGTTTCACCTGCTTCAACTAATTTTATGGCTTCTTGAATCAATGTATTCAAATCGGACCGGTCCTTTCTTTTCCTATAGAACATCAACCATAC‑AAAAAAGCTTTATTT  >  CP006881/2185256‑2185522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑