Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,172,914	C→G	L278L (TTG→CTC)	U712_10870 ←	Bifunctional protein BirA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,172,914	0	C	G	100.0%	210.4 / NA	63	L278L (TTG→CTC)	U712_10870	Bifunctional protein BirA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (30/33);  total (30/33)

GCAGAATAGATTTTTTTAATGCCTTCCTGCGTTTCTAAAAGAAGAACGCCTTCATCATCTATACCTAACGCCTTCCCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCGATTCCCAACGCATAACTTTCCCATAAAAGCTTAATCGGCGTAAAACCGTGTGTCATATAATCCCGGTACCGTTTTTCAAAGCAAAGTAAAATATGCTGGATGACGCCGGCCCGGTCAATTTTTTCTCCGGAAG  >  CP006881/2172789‑2173041                                                                                                                              |                                                                                                                                gCAGAATAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCttt                                                                                                                    >  1:247455/1‑139 (MQ=255)       tAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATa                                                                                                              <  1:133899/139‑1 (MQ=255)       tAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATa                                                                                                              <  8:94125/139‑1 (MQ=255)       tAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATa                                                                                                              <  6:401223/139‑1 (MQ=255)       tAGATTTTTTTAATGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATa                                                                                                              <  5:184384/139‑1 (MQ=255)                  aaTGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAAt                                                                                                    <  1:285347/138‑1 (MQ=255)                  aaTGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAAt                                                                                                    <  4:426717/138‑1 (MQ=255)                  aaTGCCTTCGTTCGTTTCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAAt                                                                                                    <  4:345951/138‑1 (MQ=255)                                tttCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAAAACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCgtg                                                                                     >  5:25980/1‑139 (MQ=255)                                  tCTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCgtgc                                                                                    <  2:247455/138‑2 (MQ=255)                                   cTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCgtgcg                                                                                   <  6:25980/138‑3 (MQ=255)                                   cTAAAAGCAGAACGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCgtgcg                                                                                   <  3:362792/138‑3 (MQ=255)                                        agcagaaCGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCatat                                                                             <  1:32325/136‑1 (MQ=255)                                        agcagaaCGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCatat                                                                             <  2:80615/136‑1 (MQ=255)                                                  cTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCgtgcg                                                                                   >  4:104719/1‑121 (MQ=255)                                                  cTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCgtgcg                                                                                   <  3:104719/123‑3 (MQ=255)                                                      atcatcTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCg                                                                <  8:322700/138‑1 (MQ=255)                                                      atcatcTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCg                                                                <  2:261282/138‑1 (MQ=255)                                                            tataCCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTtctc                                                          >  1:124238/1‑138 (MQ=255)                                                                ccTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTcaaag                                                      <  5:199225/138‑1 (MQ=255)                                                                ccTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTcaaag                                                      >  4:387886/1‑138 (MQ=255)                                                                   aaCGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTcaaagcata                                                  >  6:110596/1‑137 (MQ=255)                                                                   aaCGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTcaaagca                                                    >  2:228886/1‑137 (MQ=255)                                                                   aaCGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTcaaag                                                      >  3:354555/1‑135 (MQ=255)                                                                   aaCGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTcaaag                                                      <  4:354555/135‑1 (MQ=255)                                                                   aaCGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACAGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATACCGGTACCGTTTCTcaaagcaa                                                   >  1:417152/1‑138 (MQ=255)                                                                    aCGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGtttct                                                           >  3:136906/1‑127 (MQ=255)                                                                    aCGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGtttct                                                           <  4:136906/129‑3 (MQ=255)                                                                    aCGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTcaaagcata                                                  <  3:152175/138‑3 (MQ=255)                                                                     cGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAAt                                                                                                    <  7:138166/87‑1 (MQ=255)                                                                     cGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAAt                                                                                                    >  8:138166/1‑87 (MQ=255)                                                                     cGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTCCCGTTTCTcaaagcatag                                                 >  5:134102/1‑138 (MQ=255)                                                                     cGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTcaaagcatag                                                 >  5:201560/1‑138 (MQ=255)                                                                     cGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTcaaagca                                                    <  4:226646/135‑1 (MQ=255)                                                                     cGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTcaaagca                                                    >  3:226646/1‑135 (MQ=255)                                                                        cTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAAt                                                                                                    <  1:369467/84‑1 (MQ=255)                                                                        cTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAAt                                                                                                    >  2:369467/1‑84 (MQ=255)                                                                        cTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTaaa                                             <  5:415100/139‑1 (MQ=255)                                                                          taccGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAAt                                                                                                    <  2:250223/80‑1 (MQ=255)                                                                          taccGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAAt                                                                                                    >  1:250223/3‑82 (MQ=255)                                                                           accGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAata                                          >  2:431417/2‑139 (MQ=255)                                                                                  aaGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGa                                   <  1:431417/139‑1 (MQ=255)                                                                                  aaGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGa                                   <  2:124238/139‑1 (MQ=255)                                                                                       tCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCg                                                                >  8:5271/1‑105 (MQ=255)                                                                                       tCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCg                                                                <  7:5271/105‑1 (MQ=255)                                                                                       tCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTaaaa                                            <  2:192435/125‑1 (MQ=255)                                                                                       tCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTaaaa                                            >  1:192435/1‑125 (MQ=255)                                                                                       tCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACg                              >  4:15395/1‑139 (MQ=255)                                                                                       tCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACg                              >  6:255653/1‑139 (MQ=255)                                                                                       tCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTACCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCAGGTACCGTTTGTCAAAGCATAGTAAAATATGCTGGATGACg                              >  5:171495/1‑139 (MQ=255)                                                                                         cGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAgcgc                                                                                                                            <  6:200309/43‑1 (MQ=255)                                                                                         cGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAgcgc                                                                                                                            >  5:200309/1‑43 (MQ=255)                                                                                         cGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGcc                            >  6:172304/1‑139 (MQ=255)                                                                                         cGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGcc                            >  8:380489/1‑139 (MQ=255)                                                                                               aCGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCg                                                                                                  >  7:248368/1‑63 (MQ=255)                                                                                               aCGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCg                                                                                                  <  8:248368/63‑1 (MQ=255)                                                                                                cGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATg                                        >  7:188008/1‑120 (MQ=255)                                                                                                cGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATg                                        <  8:188008/120‑1 (MQ=255)                                                                                                cGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGccc                       >  5:286049/1‑137 (MQ=255)                                                                                                     tGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAAt                >  6:154335/1‑139 (MQ=255)                                                                                                             atatTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCgtgcg                                                                                   >  6:268629/1‑62 (MQ=255)                                                                                                             atatTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCgtgcg                                                                                   <  5:268629/64‑3 (MQ=255)                                                                                                                   gTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTccccagcag  <  5:110596/139‑6 (MQ=255)                                                                                                                              |                                                                                                                                GCAGAATAGATTTTTTTAATGCCTTCCTGCGTTTCTAAAAGAAGAACGCCTTCATCATCTATACCTAACGCCTTCCCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCGATTCCCAACGCATAACTTTCCCATAAAAGCTTAATCGGCGTAAAACCGTGTGTCATATAATCCCGGTACCGTTTTTCAAAGCAAAGTAAAATATGCTGGATGACGCCGGCCCGGTCAATTTTTTCTCCGGAAG  >  CP006881/2172789‑2173041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑