| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,185,850 | A→G | F417F (TTT→TTC) | U712_10955 ← | 3‑phosphoshikimate 1‑carboxyvinyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,185,850 | 0 | A | G | 94.6% | 105.0 / ‑2.3 | 37 | F417F (TTT→TTC) | U712_10955 | 3‑phosphoshikimate 1‑carboxyvinyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (12/23); total (13/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
AGCGCATCCCCCCTTTTCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCTTCAGTTATACACGAAGCGATACCAAGCATCATTCCGATTCGATGATCTCCATGGCTGGACACTGTTGCGCCGCCTCTC > CP006881/2185700‑2185982 | aGCACATCCCCCCTTTTCG‑TTTTTCTATCCGTTCTATAAGACAAGCTATGTTTTTTCGCCTCGAATTATGA‑‑‑‑‑‑‑‑‑‑‑‑‑TCTGCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGaagaag > 1:135544/1‑139 (MQ=255)aGCACATCCCCCCTTTTCG‑TTTTTCTATCCGTTCTATAAGACAAGCTATGTTTTTTCGCCTCGAATTATGA‑‑‑‑‑‑‑‑‑‑‑‑‑TCTGCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGaagaag > 4:207313/1‑139 (MQ=255) cacATCCCCCCTTTTCG‑TTTTTCTATCCGTTCTATAAGACAAGCTATGTTTTTTCGCCTCGAATTATGA‑‑‑‑‑‑‑‑‑‑‑‑‑TCTGCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGt < 5:73831/137‑1 (MQ=255) cacATCCCCCCTTTTCG‑TTTTTCTATCCGTTCTATAAGACAAGCTATGTTTTTTCGCCTCGAATTATGA‑‑‑‑‑‑‑‑‑‑‑‑‑TCTGCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGt < 4:408029/137‑1 (MQ=255) ccccccTTTTCG‑TTTTTCTATCCGTTCTATAAGACAAGCTATGTTTTTTCGCCTCGAATTATGA‑‑‑‑‑‑‑‑‑‑‑‑‑TCTGCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGAt > 8:80215/1‑139 (MQ=255) gCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGt < 6:35321/111‑1 (MQ=255) gCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGt > 5:35321/1‑111 (MQ=255) atgatCTGCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAg > 3:281509/5‑139 (MQ=255) atgatCTGCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAg > 3:287422/5‑139 (MQ=255) tgatCTGCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAg < 8:250184/135‑1 (MQ=255) tgatCTGCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAg < 7:210510/135‑1 (MQ=255) tgatCTGCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAg < 6:228438/135‑1 (MQ=255) tgatCTGCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAg < 3:315626/135‑1 (MQ=255) tgatCTGCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAg < 4:157767/135‑1 (MQ=255) tGCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGctcct > 4:291376/1‑116 (MQ=255) tGCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGctcct < 3:291376/118‑3 (MQ=255) tGCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTGGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATAc > 3:82397/1‑139 (MQ=255) gCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGtt < 4:291729/122‑1 (MQ=255) gCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGtt > 3:291729/1‑122 (MQ=255) cataaaaatTTCTGAATTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCTAAGAAGGTTGGATATGAATCGTGAATGGCATGCGTGTGCTCGATTTCAATCGGCTGCTCCGTTATACAGGAAGCAATAcc < 1:153485/135‑1 (MQ=255) cataaaaaaTCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATAcc > 5:37440/4‑138 (MQ=255) cataaaaaaTCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATAcc < 7:123030/135‑1 (MQ=255) cataaaaaaTCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATAcc > 1:153181/4‑138 (MQ=255) cataaaaaaTCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATAcc < 3:348155/135‑1 (MQ=255) cataaaaaaTCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCAGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCa < 3:248194/136‑1 (MQ=255) cataaaaaaTCCTGAA‑GTAAATCTTCAGGATTTTTTCGTTAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGTCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATAcc < 2:67735/135‑1 (MQ=255) ccTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCt < 2:329713/88‑1 (MQ=255) ccTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGGGAATGGCATCCGTGTGCt > 1:329713/1‑88 (MQ=255) aagtaaaaCTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCg < 3:207313/133‑1 (MQ=255) agtaaaaCTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGAtt < 4:220739/136‑1 (MQ=255) aaacTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCgat > 6:128842/4‑139 (MQ=255) tCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGAGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGAtct > 7:99958/1‑139 (MQ=255) aGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGAtctc < 6:37440/138‑1 (MQ=255) ggATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTccg < 2:235710/139‑2 (MQ=255) cgAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGa < 7:199228/137‑1 (MQ=255) cgAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGa < 7:428395/137‑1 (MQ=255) cgAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGa < 8:370249/137‑1 (MQ=255) tttAAATGCTCGAAGAAGGTTGGATAAGAAAAGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGAAATAACAAGCATCATTCCGATTCGATTATCTCCGTGGCTGGACACTgcagctc < 3:120465/139‑7 (MQ=255) tCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCtttc > 2:14393/1‑138 (MQ=255) | AGCGCATCCCCCCTTTTCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCTTCAGTTATACACGAAGCGATACCAAGCATCATTCCGATTCGATGATCTCCATGGCTGGACACTGTTGCGCCGCCTCTC > CP006881/2185700‑2185982 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |