Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,185,907	A→C	T398T (ACT→ACG)	U712_10955 ←	3‑phosphoshikimate 1‑carboxyvinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,185,907	0	A	C	100.0%	161.1 / NA	51	T398T (ACT→ACG)	U712_10955	3‑phosphoshikimate 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (17/34);  total (17/34)

GCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCTTCAGTTATACACGAAGCGATACCAAGCATCATTCCGATTCGATGATCTCCATGGCTGGACACTGTTGCGCCGCCTCTCAGTGTTTGCTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATCTCAGCAC  >  CP006881/2185781‑2186037                                                                                                                               |                                                                                                                                   atgatCTGCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAg                                                                                                                       >  3:287422/5‑139 (MQ=255) atgatCTGCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAg                                                                                                                       >  3:281509/5‑139 (MQ=255)  tgatCTGCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAg                                                                                                                       <  4:157767/135‑1 (MQ=255)  tgatCTGCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAg                                                                                                                       <  7:210510/135‑1 (MQ=255)  tgatCTGCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAg                                                                                                                       <  3:315626/135‑1 (MQ=255)  tgatCTGCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAg                                                                                                                       <  6:228438/135‑1 (MQ=255)  tgatCTGCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAg                                                                                                                       <  8:250184/135‑1 (MQ=255)       tGCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTGGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATAc                                                                                                                 >  3:82397/1‑139 (MQ=255)        gCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGtt                                                                                                                                 <  4:291729/122‑1 (MQ=255)        gCATAAAAAATCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGtt                                                                                                                                 >  3:291729/1‑122 (MQ=255)         cataaaaatTTCTGAATTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCTAAGAAGGTTGGATATGAATCGTGAATGGCATGCGTGTGCTCGATTTCAATCGGCTGCTCCGTTATACAGGAAGCAATAcc                                                                                                                <  1:153485/135‑1 (MQ=255)         cataaaaaaTCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATAcc                                                                                                                >  5:37440/4‑138 (MQ=255)         cataaaaaaTCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATAcc                                                                                                                <  7:123030/135‑1 (MQ=255)         cataaaaaaTCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATAcc                                                                                                                <  3:348155/135‑1 (MQ=255)         cataaaaaaTCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATAcc                                                                                                                >  1:153181/4‑138 (MQ=255)         cataaaaaaTCCTGAAGTAAAA‑CTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCAGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCa                                                                                                               <  3:248194/136‑1 (MQ=255)         cataaaaaaTCCTGAA‑GTAAATCTTCAGGATTTTTTCGTTAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGTCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATAcc                                                                                                                <  2:67735/135‑1 (MQ=255)                        aagtaaaaCTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCg                                                                                                   <  3:207313/133‑1 (MQ=255)                         agtaaaaCTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGAtt                                                                                                <  4:220739/136‑1 (MQ=255)                             aaacTTCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCgat                                                                                            >  6:128842/4‑139 (MQ=255)                                  tCAGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGAGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGAtct                                                                                       >  7:99958/1‑139 (MQ=255)                                    aGGATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGAtctc                                                                                      <  6:37440/138‑1 (MQ=255)                                     ggATTTTTTCGAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTccg                                                                                    <  2:235710/139‑2 (MQ=255)                                              cgAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGa                                                                            <  7:428395/137‑1 (MQ=255)                                              cgAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGa                                                                            <  7:199228/137‑1 (MQ=255)                                              cgAAAGCTTATTTAAATGCTCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGa                                                                            <  8:370249/137‑1 (MQ=255)                                                        tttAAATGCTCGAAGAAGGTTGGATAAGAAAAGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGAAATAACAAGCATCATTCCGATTCGATTATCTCCGTGGCTGGACACTgcagctc                                                                 <  3:120465/139‑7 (MQ=255)                                                                 tCGAAGAAGGTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCtttc                                                         >  2:14393/1‑138 (MQ=255)                                                                          gTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTttgtt                                               <  1:14393/139‑3 (MQ=255)                                                                          gTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTttgtt                                               >  3:201102/1‑137 (MQ=255)                                                                          gTTGGATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTttgtt                                               <  4:82397/139‑3 (MQ=255)                                                                              gATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGAtctc                                                                                      <  5:232981/96‑1 (MQ=255)                                                                              gATAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGAtctc                                                                                      >  6:232981/1‑96 (MQ=255)                                                                               aTAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCa                                          <  2:316471/139‑1 (MQ=255)                                                                                  atataCGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCTCCTTTCAACGTTTGTTTGACATa                                        <  3:343508/134‑1 (MQ=255)                                                                                  aGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATa                                        <  5:128842/138‑1 (MQ=255)                                                                                  aGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATa                                        <  7:49515/138‑1 (MQ=255)                                                                                  aGAAACGTGAATCGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATa                                        <  4:429507/138‑1 (MQ=255)                                                                                   gAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTGTCAACGTTTGTTTGCCATaaa                                      >  4:94415/1‑139 (MQ=255)                                                                                      aCGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCt                                   <  4:287422/139‑1 (MQ=255)                                                                                      aCGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCt                                   <  4:281509/139‑1 (MQ=255)                                                                                           aaTGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCtttc                                                         >  7:70007/1‑112 (MQ=255)                                                                                           aaTGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCtttc                                                         <  8:70007/112‑1 (MQ=255)                                                                                                     gtgtgCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCcttt                                                          >  4:374271/4‑99 (MQ=255)                                                                                                     gtgtgCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCcttt                                                          <  3:374271/98‑3 (MQ=255)                                                                                                      tgtgCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGt                   <  6:349229/137‑1 (MQ=255)                                                                                                          cTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGt               >  5:314198/1‑139 (MQ=255)                                                                                                                  caatcGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAAttt        >  7:319080/1‑136 (MQ=255)                                                                                                                  caatcGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAAttt        >  4:34871/1‑136 (MQ=255)                                                                                                                       ggCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCAc  <  2:153181/139‑1 (MQ=255)                                                                                                                       ggCTCCTCCGTTATAACGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCAc  <  8:392068/139‑1 (MQ=255)                                                                                                                               |                                                                                                                                   GCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCTTCAGTTATACACGAAGCGATACCAAGCATCATTCCGATTCGATGATCTCCATGGCTGGACACTGTTGCGCCGCCTCTCAGTGTTTGCTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATCTCAGCAC  >  CP006881/2185781‑2186037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑