| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,185,991 | C→T | K370K (AAG→AAA) | U712_10955 ← | 3‑phosphoshikimate 1‑carboxyvinyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,185,991 | 0 | C | T | 98.2% | 161.3 / ‑5.7 | 54 | K370K (AAG→AAA) | U712_10955 | 3‑phosphoshikimate 1‑carboxyvinyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (31/22); total (31/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCTTCAGTTATACACGAAGCGATACCAAGCATCATTCCGATTCGATGATCTCCATGGCTGGACACTGTTGCGCCGCCTCTCAGTGTTTGCTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATCTCAGCACCTAGCTTACGAAGCTCAGAAACCACGGTGTCAATGCGGTTTGTTTCTTTTACTTTAAGCTCTGCCGCGTCCTTAATAACGGTGG > CP006881/2185859‑2186121 | aTAAGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCa < 2:316471/139‑1 (MQ=255) atataCGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCTCCTTTCAACGTTTGTTTGACATa < 3:343508/134‑1 (MQ=255) aGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATa < 5:128842/138‑1 (MQ=255) aGAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATa < 7:49515/138‑1 (MQ=255) aGAAACGTGAATCGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATa < 4:429507/138‑1 (MQ=255) gAAACGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTGTCAACGTTTGTTTGCCATaaa > 4:94415/1‑139 (MQ=255) aCGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCt < 4:281509/139‑1 (MQ=255) aCGTGAATGGCATCCGTGTGCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCt < 4:287422/139‑1 (MQ=255) tgtgCTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGt < 6:349229/137‑1 (MQ=255) cTCGATTTCAATCGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGt > 5:314198/1‑139 (MQ=255) caatcGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAAttt > 7:319080/1‑136 (MQ=255) caatcGGCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAAttt > 4:34871/1‑136 (MQ=255) ggCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCAc < 2:153181/139‑1 (MQ=255) ggCTCCTCCGTTATAACGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCAc < 8:392068/139‑1 (MQ=255) ttATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCAccc < 1:370813/131‑2 (MQ=255) ttATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCAccc > 2:370813/1‑130 (MQ=255) gAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTc < 2:330341/90‑1 (MQ=255) gAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTc > 1:330341/1‑90 (MQ=255) gAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCt > 5:1098/1‑136 (MQ=255) gAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCt > 6:332528/1‑136 (MQ=255) gAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCt > 6:319526/1‑136 (MQ=255) gAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGAAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTACATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGca > 7:239103/1‑135 (MQ=255) aGCAATAGCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTACATCTGCTGTGGGTTCAATTTCAGCACCCAGCATTCGAAGCTCAGa > 4:69624/1‑138 (MQ=255) aGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGa > 3:116379/1‑138 (MQ=255) aGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGa > 4:131460/1‑138 (MQ=255) aGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGa > 1:314712/1‑138 (MQ=255) aGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGa > 6:185780/1‑138 (MQ=255) aaTACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAAc > 4:407960/2‑138 (MQ=255) aaTACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAAc > 7:55746/2‑138 (MQ=255) aaTACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAAc > 8:217664/2‑138 (MQ=255) aTACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAAcaa < 5:185780/139‑3 (MQ=255) aTACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAAcaa < 7:217664/139‑3 (MQ=255) tACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAAcaa > 8:397866/1‑136 (MQ=255) aCCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAAcaa > 8:233294/1‑135 (MQ=255) aGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAAcaa > 1:12034/1‑131 (MQ=255) aGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAAcaa < 2:12034/133‑3 (MQ=255) aGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAgtat > 2:257344/1‑137 (MQ=255) catTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAAta > 8:67996/1‑138 (MQ=255) ttCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAAt < 8:32396/136‑1 (MQ=255) ttCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAAt > 7:32396/1‑136 (MQ=255) ttCCGATACGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTATGTTTGCCATAAACCTTCATTCCATCTGCAGTCGGTTCAATTTAAGCACCCAGCAAGAGAAGCTCATAAACAACAGAATCAAtaac > 8:195839/1‑136 (MQ=255) gATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGgttt < 7:233294/139‑1 (MQ=255) tgatCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCtt < 5:332528/139‑1 (MQ=255) tctcCGTGGCTGGACACTGCAGCGCCGCCGTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCTATTTAAGCCCCCCGCTTGCGAAGCTCAGAAACCACAGTATCAAAACGGTTTGTTTCtttca > 2:196123/1‑137 (MQ=255) ctcCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCAc > 2:13220/1‑139 (MQ=255) cgtGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCATCACCCAGCTTGCGAATCTCAGAAACAACAGTAACAAGAAGGGTTGTTTATTTCAAttt > 8:279771/3‑139 (MQ=255) cgtGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCtttc < 5:319526/132‑2 (MQ=255) gACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTg > 8:57636/1‑139 (MQ=255) cacTGCAGCGCCGCCGTTCAACGTTTGCTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGcc < 1:432495/139‑1 (MQ=255) tgcagcgcCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCcgcg > 7:404003/5‑139 (MQ=255) tgcagcgcCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCcgcg > 4:144052/5‑139 (MQ=255) cgccTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTtaat < 7:397866/139‑1 (MQ=255) cgccTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTtaat < 3:34871/139‑1 (MQ=255) cgccTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTtaat < 1:13220/139‑1 (MQ=255) tcaacgtttgtttGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACg < 4:201102/132‑1 (MQ=255) caacgtttgtttGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGcc > 7:406116/5‑121 (MQ=255) caacgtttgtttGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGcc < 8:406116/117‑1 (MQ=255) aacgtttgtttGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACggtgg < 7:279771/136‑1 (MQ=255) aacgtttgtttGCCATAAACCTTCATTCCATCTGCTGTCGGGTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACggtgg < 3:245276/136‑1 (MQ=255) ttgtttgcCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCcgc < 2:104993/117‑1 (MQ=255) ttgtttgcCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCcgc > 1:104993/1‑117 (MQ=255) | ATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCTTCAGTTATACACGAAGCGATACCAAGCATCATTCCGATTCGATGATCTCCATGGCTGGACACTGTTGCGCCGCCTCTCAGTGTTTGCTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATCTCAGCACCTAGCTTACGAAGCTCAGAAACCACGGTGTCAATGCGGTTTGTTTCTTTTACTTTAAGCTCTGCCGCGTCCTTAATAACGGTGG > CP006881/2185859‑2186121 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |