Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,186,030	C→T	E357E (GAG→GAA)	U712_10955 ←	3‑phosphoshikimate 1‑carboxyvinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,186,030	0	C	T	100.0%	212.1 / NA	67	E357E (GAG→GAA)	U712_10955	3‑phosphoshikimate 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/26);  total (41/26)

GGCTCTTCAGTTATACACGAAGCGATACCAAGCATCATTCCGATTCGATGATCTCCATGGCTGGACACTGTTGCGCCGCCTCTCAGTGTTTGCTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATCTCAGCACCTAGCTTACGAAGCTCAGAAACCACGGTGTCAATGCGGTTTGTTTCTTTTACTTTAAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGGGTCGCAAGAAGCGCAATGATAGGTATTTCA  >  CP006881/2185899‑2186165                                                                                                                                    |                                                                                                                                        ggCTCCTCCGTTATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCAc                                                                                                                                  <  2:153181/139‑1 (MQ=255) ggCTCCTCCGTTATAACGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCAc                                                                                                                                  <  8:392068/139‑1 (MQ=255)           ttATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCAccc                                                                                                                                <  1:370813/131‑2 (MQ=255)           ttATACAGGAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCAccc                                                                                                                                >  2:370813/1‑130 (MQ=255)                   gAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCt                                                                                                                   >  5:1098/1‑136 (MQ=255)                   gAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCt                                                                                                                   >  6:319526/1‑136 (MQ=255)                   gAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCt                                                                                                                   >  6:332528/1‑136 (MQ=255)                   gAAGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGAAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTACATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGca                                                                                                                   >  7:239103/1‑135 (MQ=255)                     aGCAATAGCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTACATCTGCTGTGGGTTCAATTTCAGCACCCAGCATTCGAAGCTCAGa                                                                                                               >  4:69624/1‑138 (MQ=255)                     aGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGa                                                                                                               >  4:131460/1‑138 (MQ=255)                     aGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGa                                                                                                               >  3:116379/1‑138 (MQ=255)                     aGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGa                                                                                                               >  6:185780/1‑138 (MQ=255)                     aGCAATACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGa                                                                                                               >  1:314712/1‑138 (MQ=255)                        aaTACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAAc                                                                                                            >  7:55746/2‑138 (MQ=255)                        aaTACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAAc                                                                                                            >  8:217664/2‑138 (MQ=255)                        aaTACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAAc                                                                                                            >  4:407960/2‑138 (MQ=255)                         aTACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAAcaa                                                                                                          <  7:217664/139‑3 (MQ=255)                         aTACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAAcaa                                                                                                          <  5:185780/139‑3 (MQ=255)                          tACCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAAcaa                                                                                                          >  8:397866/1‑136 (MQ=255)                           aCCAAGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAAcaa                                                                                                          >  8:233294/1‑135 (MQ=255)                               aGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAAcaa                                                                                                          <  2:12034/133‑3 (MQ=255)                               aGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAAcaa                                                                                                          >  1:12034/1‑131 (MQ=255)                               aGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAgtat                                                                                                    >  2:257344/1‑137 (MQ=255)                                    catTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAAta                                                                                               >  8:67996/1‑138 (MQ=255)                                      ttCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAAt                                                                                                >  7:32396/1‑136 (MQ=255)                                      ttCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAAt                                                                                                <  8:32396/136‑1 (MQ=255)                                      ttCCGATACGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTATGTTTGCCATAAACCTTCATTCCATCTGCAGTCGGTTCAATTTAAGCACCCAGCAAGAGAAGCTCATAAACAACAGAATCAAtaac                                                                                             >  8:195839/1‑136 (MQ=255)                                          gATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGgttt                                                                                         <  7:233294/139‑1 (MQ=255)                                                 tgatCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCtt                                                                                  <  5:332528/139‑1 (MQ=255)                                                    tctcCGTGGCTGGACACTGCAGCGCCGCCGTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCTATTTAAGCCCCCCGCTTGCGAAGCTCAGAAACCACAGTATCAAAACGGTTTGTTTCtttca                                                                               >  2:196123/1‑137 (MQ=255)                                                     ctcCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCAc                                                                              >  2:13220/1‑139 (MQ=255)                                                        cgtGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCATCACCCAGCTTGCGAATCTCAGAAACAACAGTAACAAGAAGGGTTGTTTATTTCAAttt                                                                           >  8:279771/3‑139 (MQ=255)                                                        cgtGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCtttc                                                                                <  5:319526/132‑2 (MQ=255)                                                                gACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTg                                                                   >  8:57636/1‑139 (MQ=255)                                                                  cacTGCAGCGCCGCCGTTCAACGTTTGCTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGcc                                                                 <  1:432495/139‑1 (MQ=255)                                                                     tgcagcgcCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCcgcg                                                              >  4:144052/5‑139 (MQ=255)                                                                     tgcagcgcCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCcgcg                                                              >  7:404003/5‑139 (MQ=255)                                                                             cgccTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTtaat                                                      <  3:34871/139‑1 (MQ=255)                                                                             cgccTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTtaat                                                      <  7:397866/139‑1 (MQ=255)                                                                             cgccTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTtaat                                                      <  1:13220/139‑1 (MQ=255)                                                                                   tcaacgtttgtttGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACg                                                  <  4:201102/132‑1 (MQ=255)                                                                                    caacgtttgtttGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGcc                                                                 >  7:406116/5‑121 (MQ=255)                                                                                    caacgtttgtttGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGcc                                                                 <  8:406116/117‑1 (MQ=255)                                                                                     aacgtttgtttGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACggtgg                                              <  7:279771/136‑1 (MQ=255)                                                                                     aacgtttgtttGCCATAAACCTTCATTCCATCTGCTGTCGGGTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACggtgg                                              <  3:245276/136‑1 (MQ=255)                                                                                          ttgtttgcCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCcgc                                                               >  1:104993/1‑117 (MQ=255)                                                                                          ttgtttgcCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCcgc                                                               <  2:104993/117‑1 (MQ=255)                                                                                            gtttgcCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGttccttc                                       >  5:299554/3‑139 (MQ=255)                                                                                                      aaCCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGt                              <  3:94415/138‑1 (MQ=255)                                                                                                            cATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCaag                        >  6:438726/1‑138 (MQ=255)                                                                                                            cATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCaag                        >  2:288657/1‑138 (MQ=255)                                                                                                            cATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGAGGTTCCTTCCGCCTGAGTCGCaag                        >  2:328856/1‑138 (MQ=255)                                                                                                              ttCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCaagaag                     <  7:400632/139‑1 (MQ=255)                                                                                                                 cATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAgcg                   <  1:38194/138‑1 (MQ=255)                                                                                                                   tCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGttcct                                         >  2:371301/1‑114 (MQ=255)                                                                                                                   tCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGttcct                                         <  1:371301/114‑1 (MQ=255)                                                                                                                   tCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAgcgcga                >  7:432315/1‑137 (MQ=255)                                                                                                                   tCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAgcgcga                >  4:206543/1‑137 (MQ=255)                                                                                                                   tCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAgcgc                  >  8:193123/1‑137 (MQ=255)                                                                                                                   tCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGGCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAgcgc                  >  8:405517/1‑137 (MQ=255)                                                                                                                         gTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCt                                  >  1:414080/1‑115 (MQ=255)                                                                                                                         gTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCt                                  <  2:414080/115‑1 (MQ=255)                                                                                                                          tCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATa           <  8:55746/137‑1 (MQ=255)                                                                                                                           cGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAggg        <  3:219273/139‑2 (MQ=255)                                                                                                                            ggTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAggga       >  5:132644/1‑137 (MQ=255)                                                                                                                                 aaTTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTATTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGAtcta   >  7:110785/1‑135 (MQ=255)                                                                                                                                 aaTTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACACTATAAATACCGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAAAAACCGTTTTTCCTTCCGACATAGTCGCAAGAAGCGCCAAGATAGGGAtcaca  >  4:219273/1‑135 (MQ=255)                                                                                                                                    |                                                                                                                                        GGCTCTTCAGTTATACACGAAGCGATACCAAGCATCATTCCGATTCGATGATCTCCATGGCTGGACACTGTTGCGCCGCCTCTCAGTGTTTGCTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATCTCAGCACCTAGCTTACGAAGCTCAGAAACCACGGTGTCAATGCGGTTTGTTTCTTTTACTTTAAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGGGTCGCAAGAAGCGCAATGATAGGTATTTCA  >  CP006881/2185899‑2186165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑