Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,186,063	G→A	T346T (ACC→ACT)	U712_10955 ←	3‑phosphoshikimate 1‑carboxyvinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,186,063	0	G	A	100.0%	186.6 / NA	60	T346T (ACC→ACT)	U712_10955	3‑phosphoshikimate 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/30);  total (30/30)

AGCATCATTCCGATTCGATGATCTCCATGGCTGGACACTGTTGCGCCGCCTCTCAGTGTTTGCTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATCTCAGCACCTAGCTTACGAAGCTCAGAAACCACGGTGTCAATGCGGTTTGTTTCTTTTACTTTAAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGGGTCGCAAGAAGCGCAATGATAGGTATTTCATCAATTAAACGCGGAATGATATCTCCACC  >  CP006881/2185929‑2186194                                                                                                                                       |                                                                                                                                    aGCATCATTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAgtat                                                                                                                                 >  2:257344/1‑137 (MQ=255)      catTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAAta                                                                                                                            >  8:67996/1‑138 (MQ=255)        ttCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAAt                                                                                                                             >  7:32396/1‑136 (MQ=255)        ttCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAAt                                                                                                                             <  8:32396/136‑1 (MQ=255)        ttCCGATACGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTATGTTTGCCATAAACCTTCATTCCATCTGCAGTCGGTTCAATTTAAGCACCCAGCAAGAGAAGCTCATAAACAACAGAATCAAtaac                                                                                                                          >  8:195839/1‑136 (MQ=255)            gATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGgttt                                                                                                                      <  7:233294/139‑1 (MQ=255)                   tgatCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCtt                                                                                                               <  5:332528/139‑1 (MQ=255)                      tctcCGTGGCTGGACACTGCAGCGCCGCCGTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCTATTTAAGCCCCCCGCTTGCGAAGCTCAGAAACCACAGTATCAAAACGGTTTGTTTCtttca                                                                                                            >  2:196123/1‑137 (MQ=255)                       ctcCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCAc                                                                                                           >  2:13220/1‑139 (MQ=255)                          cgtGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCATCACCCAGCTTGCGAATCTCAGAAACAACAGTAACAAGAAGGGTTGTTTATTTCAAttt                                                                                                        >  8:279771/3‑139 (MQ=255)                          cgtGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCtttc                                                                                                             <  5:319526/132‑2 (MQ=255)                                  gACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTg                                                                                                >  8:57636/1‑139 (MQ=255)                                    cacTGCAGCGCCGCCGTTCAACGTTTGCTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGcc                                                                                              <  1:432495/139‑1 (MQ=255)                                       tgcagcgcCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCcgcg                                                                                           >  7:404003/5‑139 (MQ=255)                                       tgcagcgcCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCcgcg                                                                                           >  4:144052/5‑139 (MQ=255)                                               cgccTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTtaat                                                                                   <  7:397866/139‑1 (MQ=255)                                               cgccTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTtaat                                                                                   <  1:13220/139‑1 (MQ=255)                                               cgccTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTtaat                                                                                   <  3:34871/139‑1 (MQ=255)                                                     tcaacgtttgtttGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACg                                                                               <  4:201102/132‑1 (MQ=255)                                                      caacgtttgtttGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGcc                                                                                              >  7:406116/5‑121 (MQ=255)                                                      caacgtttgtttGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGcc                                                                                              <  8:406116/117‑1 (MQ=255)                                                       aacgtttgtttGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACggtgg                                                                           <  7:279771/136‑1 (MQ=255)                                                       aacgtttgtttGCCATAAACCTTCATTCCATCTGCTGTCGGGTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACggtgg                                                                           <  3:245276/136‑1 (MQ=255)                                                            ttgtttgcCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCcgc                                                                                            >  1:104993/1‑117 (MQ=255)                                                            ttgtttgcCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCcgc                                                                                            <  2:104993/117‑1 (MQ=255)                                                              gtttgcCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGttccttc                                                                    >  5:299554/3‑139 (MQ=255)                                                                        aaCCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGt                                                           <  3:94415/138‑1 (MQ=255)                                                                              cATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCaag                                                     >  6:438726/1‑138 (MQ=255)                                                                              cATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCaag                                                     >  2:288657/1‑138 (MQ=255)                                                                              cATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGAGGTTCCTTCCGCCTGAGTCGCaag                                                     >  2:328856/1‑138 (MQ=255)                                                                                ttCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCaagaag                                                  <  7:400632/139‑1 (MQ=255)                                                                                   cATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAgcg                                                <  1:38194/138‑1 (MQ=255)                                                                                     tCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGttcct                                                                      <  1:371301/114‑1 (MQ=255)                                                                                     tCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGttcct                                                                      >  2:371301/1‑114 (MQ=255)                                                                                     tCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAgcgcga                                             >  7:432315/1‑137 (MQ=255)                                                                                     tCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAgcgcga                                             >  4:206543/1‑137 (MQ=255)                                                                                     tCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAgcgc                                               >  8:193123/1‑137 (MQ=255)                                                                                     tCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGGCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAgcgc                                               >  8:405517/1‑137 (MQ=255)                                                                                           gTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCt                                                               <  2:414080/115‑1 (MQ=255)                                                                                           gTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCt                                                               >  1:414080/1‑115 (MQ=255)                                                                                            tCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATa                                        <  8:55746/137‑1 (MQ=255)                                                                                             cGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAggg                                     <  3:219273/139‑2 (MQ=255)                                                                                              ggTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAggga                                    >  5:132644/1‑137 (MQ=255)                                                                                                   aaTTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTATTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGAtcta                                >  7:110785/1‑135 (MQ=255)                                                                                                   aaTTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACACTATAAATACCGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAAAAACCGTTTTTCCTTCCGACATAGTCGCAAGAAGCGCCAAGATAGGGAtcaca                               >  4:219273/1‑135 (MQ=255)                                                                                                            aCCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGATTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTaaa                      <  6:314198/139‑1 (MQ=255)                                                                                                              ccaGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAAcg                    <  7:319159/137‑1 (MQ=255)                                                                                                              ccaGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAAcg                    <  7:193123/137‑1 (MQ=255)                                                                                                                aGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGttcct                                                                      >  4:204684/1‑87 (MQ=255)                                                                                                                aGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGttcct                                                                      <  3:204684/87‑1 (MQ=255)                                                                                                                aGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAAcgcg                  <  1:257344/139‑1 (MQ=255)                                                                                                                  cTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGa                <  1:328856/139‑1 (MQ=255)                                                                                                                     gcGAAGCTCAGAAACAACAGTATCAATACGGTTTGTGTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAAt              >  8:256604/2‑138 (MQ=255)                                                                                                                          gCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATAtc        >  3:404684/1‑139 (MQ=255)                                                                                                                            tCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGAt                                   >  2:238503/1‑110 (MQ=255)                                                                                                                            tCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGAt                                   <  1:238503/110‑1 (MQ=255)                                                                                                                            tCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATAtctc      >  3:396979/1‑139 (MQ=255)                                                                                                                              aGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTcct    <  6:1098/139‑2 (MQ=255)                                                                                                                              aGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTcct    <  4:396979/139‑2 (MQ=255)                                                                                                                                aaaCAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGGGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCtcctcc  <  2:314712/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    AGCATCATTCCGATTCGATGATCTCCATGGCTGGACACTGTTGCGCCGCCTCTCAGTGTTTGCTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATCTCAGCACCTAGCTTACGAAGCTCAGAAACCACGGTGTCAATGCGGTTTGTTTCTTTTACTTTAAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGGGTCGCAAGAAGCGCAATGATAGGTATTTCATCAATTAAACGCGGAATGATATCTCCACC  >  CP006881/2185929‑2186194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑