Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,186,066	G→A	D345D (GAC→GAT)	U712_10955 ←	3‑phosphoshikimate 1‑carboxyvinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,186,066	0	G	A	100.0%	183.4 / NA	59	D345D (GAC→GAT)	U712_10955	3‑phosphoshikimate 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/30);  total (29/30)

CATTCCGATTCGATGATCTCCATGGCTGGACACTGTTGCGCCGCCTCTCAGTGTTTGCTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATCTCAGCACCTAGCTTACGAAGCTCAGAAACCACGGTGTCAATGCGGTTTGTTTCTTTTACTTTAAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGGGTCGCAAGAAGCGCAATGATAGGTATTTCATCAATTAAACGCGGAATGATATCTCCACC  >  CP006881/2185934‑2186194                                                                                                                                     |                                                                                                                                 catTCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAAta                                                                                                                            >  8:67996/1‑138 (MQ=255)   ttCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAAt                                                                                                                             <  8:32396/136‑1 (MQ=255)   ttCCGATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAAt                                                                                                                             >  7:32396/1‑136 (MQ=255)   ttCCGATACGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTATGTTTGCCATAAACCTTCATTCCATCTGCAGTCGGTTCAATTTAAGCACCCAGCAAGAGAAGCTCATAAACAACAGAATCAAtaac                                                                                                                          >  8:195839/1‑136 (MQ=255)       gATTCGATGATCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGgttt                                                                                                                      <  7:233294/139‑1 (MQ=255)              tgatCTCCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCtt                                                                                                               <  5:332528/139‑1 (MQ=255)                 tctcCGTGGCTGGACACTGCAGCGCCGCCGTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCTATTTAAGCCCCCCGCTTGCGAAGCTCAGAAACCACAGTATCAAAACGGTTTGTTTCtttca                                                                                                            >  2:196123/1‑137 (MQ=255)                  ctcCGTGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCAc                                                                                                           >  2:13220/1‑139 (MQ=255)                     cgtGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCATCACCCAGCTTGCGAATCTCAGAAACAACAGTAACAAGAAGGGTTGTTTATTTCAAttt                                                                                                        >  8:279771/3‑139 (MQ=255)                     cgtGGCTGGACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCtttc                                                                                                             <  5:319526/132‑2 (MQ=255)                             gACACTGCAGCGCCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTg                                                                                                >  8:57636/1‑139 (MQ=255)                               cacTGCAGCGCCGCCGTTCAACGTTTGCTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGcc                                                                                              <  1:432495/139‑1 (MQ=255)                                  tgcagcgcCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCcgcg                                                                                           >  7:404003/5‑139 (MQ=255)                                  tgcagcgcCGCCTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCcgcg                                                                                           >  4:144052/5‑139 (MQ=255)                                          cgccTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTtaat                                                                                   <  1:13220/139‑1 (MQ=255)                                          cgccTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTtaat                                                                                   <  7:397866/139‑1 (MQ=255)                                          cgccTTTCAACGTTTGTTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTtaat                                                                                   <  3:34871/139‑1 (MQ=255)                                                tcaacgtttgtttGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACg                                                                               <  4:201102/132‑1 (MQ=255)                                                 caacgtttgtttGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGcc                                                                                              >  7:406116/5‑121 (MQ=255)                                                 caacgtttgtttGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGcc                                                                                              <  8:406116/117‑1 (MQ=255)                                                  aacgtttgtttGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACggtgg                                                                           <  7:279771/136‑1 (MQ=255)                                                  aacgtttgtttGCCATAAACCTTCATTCCATCTGCTGTCGGGTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACggtgg                                                                           <  3:245276/136‑1 (MQ=255)                                                       ttgtttgcCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCcgc                                                                                            >  1:104993/1‑117 (MQ=255)                                                       ttgtttgcCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCcgc                                                                                            <  2:104993/117‑1 (MQ=255)                                                         gtttgcCATAAACCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGttccttc                                                                    >  5:299554/3‑139 (MQ=255)                                                                   aaCCTTCATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGt                                                           <  3:94415/138‑1 (MQ=255)                                                                         cATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCaag                                                     >  6:438726/1‑138 (MQ=255)                                                                         cATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCaag                                                     >  2:288657/1‑138 (MQ=255)                                                                         cATTCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGAGGTTCCTTCCGCCTGAGTCGCaag                                                     >  2:328856/1‑138 (MQ=255)                                                                           ttCCATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCaagaag                                                  <  7:400632/139‑1 (MQ=255)                                                                              cATCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAgcg                                                <  1:38194/138‑1 (MQ=255)                                                                                tCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGttcct                                                                      <  1:371301/114‑1 (MQ=255)                                                                                tCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGttcct                                                                      >  2:371301/1‑114 (MQ=255)                                                                                tCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAgcgcga                                             >  7:432315/1‑137 (MQ=255)                                                                                tCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAgcgcga                                             >  4:206543/1‑137 (MQ=255)                                                                                tCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAgcgc                                               >  8:193123/1‑137 (MQ=255)                                                                                tCTGCTGTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGGCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAgcgc                                               >  8:405517/1‑137 (MQ=255)                                                                                      gTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCt                                                               <  2:414080/115‑1 (MQ=255)                                                                                      gTCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCt                                                               >  1:414080/1‑115 (MQ=255)                                                                                       tCGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATa                                        <  8:55746/137‑1 (MQ=255)                                                                                        cGGTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAggg                                     <  3:219273/139‑2 (MQ=255)                                                                                         ggTTCAATTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAggga                                    >  5:132644/1‑137 (MQ=255)                                                                                              aaTTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTATTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGAtcta                                >  7:110785/1‑135 (MQ=255)                                                                                              aaTTTCAGCACCCAGCTTGCGAAGCTCAGAAACAACACTATAAATACCGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAAAAACCGTTTTTCCTTCCGACATAGTCGCAAGAAGCGCCAAGATAGGGAtcaca                               >  4:219273/1‑135 (MQ=255)                                                                                                       aCCCAGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGATTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTaaa                      <  6:314198/139‑1 (MQ=255)                                                                                                         ccaGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAAcg                    <  7:319159/137‑1 (MQ=255)                                                                                                         ccaGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAAcg                    <  7:193123/137‑1 (MQ=255)                                                                                                           aGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGttcct                                                                      >  4:204684/1‑87 (MQ=255)                                                                                                           aGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGttcct                                                                      <  3:204684/87‑1 (MQ=255)                                                                                                           aGCTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAAcgcg                  <  1:257344/139‑1 (MQ=255)                                                                                                             cTTGCGAAGCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGa                <  1:328856/139‑1 (MQ=255)                                                                                                                gcGAAGCTCAGAAACAACAGTATCAATACGGTTTGTGTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAAt              >  8:256604/2‑138 (MQ=255)                                                                                                                     gCTCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATAtc        >  3:404684/1‑139 (MQ=255)                                                                                                                       tCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGAt                                   >  2:238503/1‑110 (MQ=255)                                                                                                                       tCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGAt                                   <  1:238503/110‑1 (MQ=255)                                                                                                                       tCAGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATAtctc      >  3:396979/1‑139 (MQ=255)                                                                                                                         aGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTcct    <  6:1098/139‑2 (MQ=255)                                                                                                                         aGAAACAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTcct    <  4:396979/139‑2 (MQ=255)                                                                                                                           aaaCAACAGTATCAATACGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGGGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCtcctcc  <  2:314712/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                 CATTCCGATTCGATGATCTCCATGGCTGGACACTGTTGCGCCGCCTCTCAGTGTTTGCTTGCCATAAACCTTCATTCCATCTGCTGTCGGTTCAATCTCAGCACCTAGCTTACGAAGCTCAGAAACCACGGTGTCAATGCGGTTTGTTTCTTTTACTTTAAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGGGTCGCAAGAAGCGCAATGATAGGTATTTCATCAATTAAACGCGGAATGATATCTCCACC  >  CP006881/2185934‑2186194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑