Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,172,959	T→C	T263T (ACA→ACG)	U712_10870 ←	Bifunctional protein BirA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,172,959	0	T	C	100.0%	193.3 / NA	60	T263T (ACA→ACG)	U712_10870	Bifunctional protein BirA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/27);  total (33/27)

AGAAGAACGCCTTCATCATCTATACCTAACGCCTTCCCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCGATTCCCAACGCATAACTTTCCCATAAAAGCTTAATCGGCGTAAAACCGTGTGTCATATAATCCCGGTACCGTTTTTCAAAGCAAAGTAAAATATGCTGGATGACGCCGGCCCGGTCAATTTTTTCTCCGGAAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAGTTCATCTGGAAAATCATCAGAC  >  CP006881/2172828‑2173094                                                                                                                                    |                                                                                                                                        agcagaaCGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCatat                                                                                                                                  <  1:32325/136‑1 (MQ=255) agcagaaCGCCTTCATCATCTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCatat                                                                                                                                  <  2:80615/136‑1 (MQ=255)               atcatcTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCg                                                                                                                     <  8:322700/138‑1 (MQ=255)               atcatcTATACCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCg                                                                                                                     <  2:261282/138‑1 (MQ=255)                     tataCCTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTtctc                                                                                                               >  1:124238/1‑138 (MQ=255)                         ccTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTcaaag                                                                                                           >  4:387886/1‑138 (MQ=255)                         ccTAACGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTcaaag                                                                                                           <  5:199225/138‑1 (MQ=255)                            aaCGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTcaaagcata                                                                                                       >  6:110596/1‑137 (MQ=255)                            aaCGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTcaaagca                                                                                                         >  2:228886/1‑137 (MQ=255)                            aaCGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTcaaag                                                                                                           <  4:354555/135‑1 (MQ=255)                            aaCGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTcaaag                                                                                                           >  3:354555/1‑135 (MQ=255)                            aaCGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACAGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATACCGGTACCGTTTCTcaaagcaa                                                                                                        >  1:417152/1‑138 (MQ=255)                             aCGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGtttct                                                                                                                <  4:136906/129‑3 (MQ=255)                             aCGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGtttct                                                                                                                >  3:136906/1‑127 (MQ=255)                             aCGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTcaaagcata                                                                                                       <  3:152175/138‑3 (MQ=255)                              cGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTCCCGTTTCTcaaagcatag                                                                                                      >  5:134102/1‑138 (MQ=255)                              cGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTcaaagcatag                                                                                                      >  5:201560/1‑138 (MQ=255)                              cGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTcaaagca                                                                                                         >  3:226646/1‑135 (MQ=255)                              cGCCTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTcaaagca                                                                                                         <  4:226646/135‑1 (MQ=255)                                 cTTACCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTaaa                                                                                                  <  5:415100/139‑1 (MQ=255)                                    accGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAata                                                                                               >  2:431417/2‑139 (MQ=255)                                           aaGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGa                                                                                        <  2:124238/139‑1 (MQ=255)                                           aaGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGa                                                                                        <  1:431417/139‑1 (MQ=255)                                                tCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCg                                                                                                                     <  7:5271/105‑1 (MQ=255)                                                tCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCg                                                                                                                     >  8:5271/1‑105 (MQ=255)                                                tCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTaaaa                                                                                                 >  1:192435/1‑125 (MQ=255)                                                tCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTaaaa                                                                                                 <  2:192435/125‑1 (MQ=255)                                                tCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACg                                                                                   >  4:15395/1‑139 (MQ=255)                                                tCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACg                                                                                   >  6:255653/1‑139 (MQ=255)                                                tCCGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTACCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCAGGTACCGTTTGTCAAAGCATAGTAAAATATGCTGGATGACg                                                                                   >  5:171495/1‑139 (MQ=255)                                                  cGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGcc                                                                                 >  8:380489/1‑139 (MQ=255)                                                  cGTTTAACGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGcc                                                                                 >  6:172304/1‑139 (MQ=255)                                                         cGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATg                                                                                             <  8:188008/120‑1 (MQ=255)                                                         cGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATg                                                                                             >  7:188008/1‑120 (MQ=255)                                                         cGTTCTGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGccc                                                                            >  5:286049/1‑137 (MQ=255)                                                              tGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAAt                                                                     >  6:154335/1‑139 (MQ=255)                                                                            gTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTccccagcag                                                       <  5:110596/139‑6 (MQ=255)                                                                                     ccgagcGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCt                                                >  7:317489/6‑137 (MQ=255)                                                                                       gagcGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCgat                                                                         >  6:42979/4‑108 (MQ=255)                                                                                       gagcGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCgat                                                                         <  5:42979/107‑3 (MQ=255)                                                                                       gagcGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAgg                                            >  3:67994/4‑139 (MQ=255)                                                                                       gagcGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAgg                                            >  5:73119/4‑139 (MQ=255)                                                                                       gagcGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAgg                                            >  3:360404/4‑139 (MQ=255)                                                                                                gcTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTcgcg                                   <  2:365564/138‑1 (MQ=255)                                                                                                 cTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGa                                  <  2:116984/139‑1 (MQ=255)                                                                                                 cTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGa                                  <  2:46896/139‑1 (MQ=255)                                                                                                    tCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGCGGCTTGTCGCGAGGt                               >  8:205954/1‑139 (MQ=255)                                                                                                    tCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGAt                                 <  7:380489/137‑1 (MQ=255)                                                                                                    tCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGAt                                 <  5:172304/137‑1 (MQ=255)                                                                                                    tCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGt                               >  2:347003/1‑139 (MQ=255)                                                                                                    tCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGt                               >  7:185751/1‑139 (MQ=255)                                                                                                           aaaGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCaa                        >  1:380279/1‑138 (MQ=255)                                                                                                               cTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTcaaagcata                                                                                                       <  7:35621/56‑3 (MQ=255)                                                                                                               cTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTcaaagcata                                                                                                       >  8:35621/1‑54 (MQ=255)                                                                                                                 tAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCagcagc                                                      >  6:276901/1‑103 (MQ=255)                                                                                                                 tAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCagcagc                                                      <  5:276901/103‑1 (MQ=255)                                                                                                                      gtcGTAAATCCGTGCGTCATATAATGCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGaa             <  2:417152/137‑1 (MQ=255)                                                                                                                      ggCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGaa             <  3:212416/139‑1 (MQ=255)                                                                                                                        cGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCCGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCTTCTGGaaaa           >  7:256821/1‑139 (MQ=255)                                                                                                                            aatccGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAatca        <  6:286049/135‑1 (MQ=255)                                                                                                                                 gtgcgtCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGAGGTCCTTCAATTCATCTGGAAAATCATTAggc  >  1:260607/1‑137 (MQ=255)                                                                                                                                    |                                                                                                                                        AGAAGAACGCCTTCATCATCTATACCTAACGCCTTCCCGTAAAAGGTTCCGTTTAACGTTCTGGCTCTCATATTAGTGCCGATTCCCAACGCATAACTTTCCCATAAAAGCTTAATCGGCGTAAAACCGTGTGTCATATAATCCCGGTACCGTTTTTCAAAGCAAAGTAAAATATGCTGGATGACGCCGGCCCGGTCAATTTTTTCTCCGGAAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAGTTCATCTGGAAAATCATCAGAC  >  CP006881/2172828‑2173094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑