Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,186,204	C→T	A299A (GCG→GCA)	U712_10955 ←	3‑phosphoshikimate 1‑carboxyvinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,186,204	0	C	T	100.0%	307.7 / NA	97	A299A (GCG→GCA)	U712_10955	3‑phosphoshikimate 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (42/55);  total (42/55)

TGCGGTTTGTTTCTTTTACTTTAAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGGGTCGCAAGAAGCGCAATGATAGGTATTTCATCAATTAAACGCGGAATGATATCTCCACCAATTTCAACCGCTTTTAGAGATGACGTTTCTATGACCAAATCACCGTAAGGCTCTGCACTGCTGTCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATGATACCTGTCCGAGTCG  >  CP006881/2186071‑2186328                                                                                                                                      |                                                                                                                             tacGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCctt                                                                                                                         <  1:223426/137‑1 (MQ=255)  acGGTTTGTTTCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCctt                                                                                                                         >  4:409039/2‑138 (MQ=255)     gtttgtttCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTTAACTGCCTTTa                                                                                                                       >  1:99920/1‑137 (MQ=255)     gtttgtttCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTa                                                                                                                       >  3:41364/1‑137 (MQ=255)     gtttgtttCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTa                                                                                                                       >  5:25307/1‑137 (MQ=255)     gtttgtttCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTa                                                                                                                       >  7:427272/1‑137 (MQ=255)        tgtttCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGAt                                                                                                                  >  3:20333/1‑139 (MQ=255)         gtttCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGAt                                                                                                                  >  4:375009/1‑138 (MQ=255)         gtttCTTTCACTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGAt                                                                                                                  >  8:375752/1‑138 (MQ=255)                  aCTTTTAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCt                                                                                                         <  7:256604/138‑1 (MQ=255)                    ttttAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTAt                                                                                                       >  1:43648/1‑138 (MQ=255)                         gcgctGCCGCGTCCTTAATAACGGTTGTTCCTTCCTCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATTATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCaa                                                                                                 <  4:102802/136‑1 (MQ=255)                         gCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCaa                                                                                                 <  6:25307/139‑1 (MQ=255)                             tGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAAt                                                                                               <  8:239103/137‑1 (MQ=255)                             tGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAAt                                                                                               <  3:131460/137‑1 (MQ=255)                             tGCCGCGTCCTTAATAACGGTGGTTCCTTCAGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAAt                                                                                               <  3:38438/137‑1 (MQ=255)                             tGCCGCGTCCTTAATAACGGTGGTGCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAAt                                                                                               <  8:99958/137‑1 (MQ=255)                             tGCCGCCTCCTTAATAACGGTGGTTCCTTCCGCCTGAGGCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCGTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAAt                                                                                               <  1:411808/137‑1 (MQ=255)                              gCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACTCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTAtaatc                                                                                                   >  5:360172/1‑128 (MQ=255)                              gCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTAtaatc                                                                                                   <  6:360172/132‑5 (MQ=255)                              gCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAAt                                                                                               <  4:195507/136‑1 (MQ=255)                              gCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAAt                                                                                               >  3:195507/1‑136 (MQ=255)                               ccGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATct                                                                                             <  8:110785/137‑2 (MQ=255)                                  cgTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTccat                                                                                         >  2:80363/1‑136 (MQ=255)                                  cgTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATa                                                                                        <  6:132644/139‑1 (MQ=255)                                  cgTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATa                                                                                        <  7:375752/139‑1 (MQ=255)                                  cgTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATa                                                                                        <  8:432315/139‑1 (MQ=255)                                  cgTCCTTAATAACGGTGGTGCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATa                                                                                        <  8:91860/139‑1 (MQ=255)                                   gTCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATaa                                                                                       <  3:144052/139‑1 (MQ=255)                                    tCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAg                                                                                      >  4:281100/1‑139 (MQ=255)                                    tCCTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAg                                                                                      >  8:116259/1‑139 (MQ=255)                                    tCCTTAATAACGGTGGTTCCTTACGCCTGAGTCGCAAGAAGCGCCAAGATAGGCAACACAACAATTAAACCCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGAAGACGTTTCAATAATCAAATCACCATAAg                                                                                      >  4:277329/1‑139 (MQ=255)                                      cTTAATAACGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAgg                                                                                     <  3:409039/138‑1 (MQ=255)                                         aataaCGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGct                                                                                   <  8:57827/137‑1 (MQ=255)                                             aCGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTTAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCa                                                                              <  2:99920/138‑1 (MQ=255)                                             aCGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCa                                                                              <  3:375009/138‑1 (MQ=255)                                             aCGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCa                                                                              <  3:277329/138‑1 (MQ=255)                                             aCGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCa                                                                              <  5:419589/138‑1 (MQ=255)                                             aCGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCa                                                                              <  7:49750/138‑1 (MQ=255)                                              cGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATa                                                                                        <  4:341653/127‑1 (MQ=255)                                              cGGTGGTTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATa                                                                                        >  3:341653/1‑127 (MQ=255)                                                gtggtTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCAccgc                                                                          >  6:137222/1‑139 (MQ=255)                                                 tggtTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCAccgct                                                                         <  3:407960/139‑1 (MQ=255)                                                 tggtTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCAccgct                                                                         <  7:116259/139‑1 (MQ=255)                                                   gtTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCAccgctat                                                                       >  4:7244/1‑137 (MQ=255)                                                   gtTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCAccgctat                                                                       >  7:74636/1‑137 (MQ=255)                                                   gtTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCAccgctat                                                                       >  7:247706/1‑137 (MQ=255)                                                   gtTCCTTCCGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCAccgctat                                                                       >  3:63361/1‑137 (MQ=255)                                                         tccGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCa                                                                              >  1:8066/1‑126 (MQ=255)                                                         tccGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCa                                                                              <  2:8066/126‑1 (MQ=255)                                                         tccGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATcag                                                                    >  6:48266/1‑136 (MQ=255)                                                          ccGCCTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATcagca                                                                  <  3:206543/137‑1 (MQ=255)                                                             ccTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATa                                                                                        <  6:354100/112‑1 (MQ=255)                                                             ccTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATa                                                                                        >  5:354100/1‑112 (MQ=255)                                                              cTGAGTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGt                                                            <  5:438726/139‑1 (MQ=255)                                                                 agTCGCAAGAAGCGCGATGATAGGGATCTCTTCAATTAAACGCGGTATGATATCTGCTCCGATTTCATCTGCCTTTAGAGATGACGTTTCTATATTCTTTTCTCCATAAGGGTCTGCTCCGCTATCAGCAGATGGTTTg                                                         >  5:359438/2‑139 (MQ=255)                                                                 agTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAAt                                                                                               >  8:238010/2‑101 (MQ=255)                                                                 agTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAAt                                                                                               <  7:238010/100‑1 (MQ=255)                                                                 agTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGttt                                                          >  1:199016/2‑138 (MQ=255)                                                                 agTCGCAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTGAGAGAACACGTTTCTATAATCAAATCTCCATATGGCTCTGCACCGCTATCAGCAGATGGatt                                                          >  5:365445/2‑138 (MQ=255)                                                                 agTCGCAAGAAGCGCGAGGATAGGGCTCTCATCAATTAAACGCGGAATCATATCTCCTCCGATTTCAACTGCCTTTAGAGATCACGTTTCTATAATCAAATCTCCATAATGCTCTGCACCGCTATCACAAGATGGttt                                                          >  2:366445/2‑138 (MQ=255)                                                                      cAAGAAGCGCGATGATAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTc                                                    <  7:57636/139‑1 (MQ=255)                                                                                    aTAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGccccc                                       <  7:405517/138‑1 (MQ=255)                                                                                    aTAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCGCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCa                                      <  2:289312/139‑1 (MQ=255)                                                                                     tAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCAccgctat                                                                       <  3:366628/105‑3 (MQ=255)                                                                                     tAGGGATCTCATCAATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCAccgctat                                                                       >  4:366628/1‑103 (MQ=255)                                                                                            ctcatcaATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTg                               >  3:132650/2‑138 (MQ=255)                                                                                            ctcatcaATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTGTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTg                               >  8:257366/2‑138 (MQ=255)                                                                                              catcaATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATcagca                                                                  >  8:128292/1‑101 (MQ=255)                                                                                              catcaATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATcagca                                                                  <  7:128292/101‑1 (MQ=255)                                                                                              catcaATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAg                            >  1:2527/1‑139 (MQ=255)                                                                                                 caATTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGAc                         <  1:288657/139‑1 (MQ=255)                                                                                                  aaTTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGAc                         <  4:41364/138‑1 (MQ=255)                                                                                                  aaTTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGAc                         <  4:20333/138‑1 (MQ=255)                                                                                                  aaTTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGAc                         <  3:7244/138‑1 (MQ=255)                                                                                                  aaTTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGAc                         <  5:137222/138‑1 (MQ=255)                                                                                                  aaTTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGAc                         <  2:2527/138‑1 (MQ=255)                                                                                                  aaTTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACa                        <  2:199016/139‑1 (MQ=255)                                                                                                  aaTTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACa                        <  3:91410/139‑1 (MQ=255)                                                                                                  aaTTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTAAAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGAc                         <  8:427272/138‑1 (MQ=255)                                                                                                   aTTAAACGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACAt                       >  4:351206/1‑139 (MQ=255)                                                                                                       aaCGCGGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAAt                   <  4:63361/139‑1 (MQ=255)                                                                                                         cgcgGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTg                                            >  3:11333/1‑112 (MQ=255)                                                                                                         cgcgGAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTg                                            <  4:11333/112‑1 (MQ=255)                                                                                                            ggAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGAc                         <  1:183402/128‑1 (MQ=255)                                                                                                            ggAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGAc                         >  2:183402/1‑128 (MQ=255)                                                                                                            ggAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATAc              <  4:132650/139‑1 (MQ=255)                                                                                                                  atatCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTcc        <  1:80363/139‑1 (MQ=255)                                                                                                                      ctcctcCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGt    >  8:234980/1‑139 (MQ=255)                                                                                                                        cctccgaTTTCATCTGCCTTTAGAGATGACGTTACTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGAAGCTTTGATTTCACGTTTTGCCCCCATGTTTTGAGGGCCCTCAATATTACCTGTCCTAGTCg  >  8:104782/7‑139 (MQ=255)                                                                                                                        cctccgaTTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCg  >  1:18040/7‑139 (MQ=255)                                                                                                                         ctgcgaTTTCTACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACAGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGt    <  1:366445/131‑1 (MQ=255)                                                                                                                         ctccgaTTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGt    <  3:281100/131‑1 (MQ=255)                                                                                                                              aTTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCa                                                                              <  3:260694/57‑1 (MQ=255)                                                                                                                              aTTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCa                                                                              >  4:260694/1‑57 (MQ=255)                                                                                                                                   aaCTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGAc                         <  6:300602/105‑1 (MQ=255)                                                                                                                                   aaCTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGAc                         >  5:300602/1‑105 (MQ=255)                                                                                                                                      |                                                                                                                             TGCGGTTTGTTTCTTTTACTTTAAGCTCTGCCGCGTCCTTAATAACGGTGGTTCCTTCCGCCTGGGTCGCAAGAAGCGCAATGATAGGTATTTCATCAATTAAACGCGGAATGATATCTCCACCAATTTCAACCGCTTTTAGAGATGACGTTTCTATGACCAAATCACCGTAAGGCTCTGCACTGCTGTCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATGATACCTGTCCGAGTCG  >  CP006881/2186071‑2186328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑