Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,186,312	G→A	I263I (ATC→ATT)	U712_10955 ←	3‑phosphoshikimate 1‑carboxyvinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,186,312	0	G	A	100.0%	192.7 / NA	62	I263I (ATC→ATT)	U712_10955	3‑phosphoshikimate 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/25);  total (37/25)

GGAATGATATCTCCACCAATTTCAACCGCTTTTAGAGATGACGTTTCTATGACCAAATCACCGTAAGGCTCTGCACTGCTGTCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATGATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTTAATACAATCTTACTGTTTGGAACCATTGCACCCGCAATAAGGAAAAACGCCGCTGAAGAAATGTCTCCAGGAACAAAAATATCGGCAGCTGTCAATTTC  >  CP006881/2186178‑2186447                                                                                                                                       |                                                                                                                                        ggAATGATATCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATAc                                                                                                                                     <  4:132650/139‑1 (MQ=255)       atatCTCCTCCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTcc                                                                                                                               <  1:80363/139‑1 (MQ=255)           ctcctcCGATTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGt                                                                                                                           >  8:234980/1‑139 (MQ=255)             cctccgaTTTCATCTGCCTTTAGAGATGACGTTACTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGAAGCTTTGATTTCACGTTTTGCCCCCATGTTTTGAGGGCCCTCAATATTACCTGTCCTAGTCg                                                                                                                         >  8:104782/7‑139 (MQ=255)             cctccgaTTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCg                                                                                                                         >  1:18040/7‑139 (MQ=255)              ctgcgaTTTCTACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACAGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGt                                                                                                                           <  1:366445/131‑1 (MQ=255)              ctccgaTTTCAACTGCCTTTAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGt                                                                                                                           <  3:281100/131‑1 (MQ=255)                                 tAGAGATGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGttttt                                                                                                      <  2:385549/138‑1 (MQ=255)                                   gagaTGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCaa                                                                                                   <  8:247706/139‑1 (MQ=255)                                   gagaTGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGGTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCaa                                                                                                   <  8:74636/139‑1 (MQ=255)                                     gaTGACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTG‑CCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTATACCTACGTTTTTCAATa                                                                                                 <  3:169360/138‑1 (MQ=255)                                        gACGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACaa                                                                                              >  5:184697/1‑139 (MQ=255)                                         aCGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCATTAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAAt                                                                                             >  8:332967/1‑139 (MQ=255)                                         aCGTTTCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACaa                                                                                              <  6:365445/138‑1 (MQ=255)                                         aCGTTTCAATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTATGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACaa                                                                                              <  6:359438/138‑1 (MQ=255)                                              tCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAAttctg                                                                                         <  7:257366/138‑5 (MQ=255)                                              tCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAAttctg                                                                                         <  2:43648/138‑5 (MQ=255)                                              tCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAAttctg                                                                                         <  6:299554/138‑5 (MQ=255)                                              tCTATAATCAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAAttctg                                                                                         <  5:48266/138‑5 (MQ=255)                                              tCTATAATCAAATCTCCATAAGGCTCTGCACCGCTAGCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAAttctg                                                                                         <  7:67996/138‑5 (MQ=255)                                                     tcAAATCTCCATAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTg                                                                                  >  3:421283/2‑138 (MQ=255)                                                               atAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGttttt                                                                                                      >  8:244458/2‑108 (MQ=255)                                                               atAAGGCTCTGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGttttt                                                                                                      <  7:244458/107‑1 (MQ=255)                                                                        tGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGAtt                                                                                                                     <  5:282275/84‑1 (MQ=255)                                                                        tGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGAtt                                                                                                                     >  6:282275/1‑84 (MQ=255)                                                                        tGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAAc                                                                                                                >  1:46315/1‑89 (MQ=255)                                                                        tGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAAc                                                                                                                <  2:46315/89‑1 (MQ=255)                                                                        tGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACg                                                                                                           >  6:339028/1‑94 (MQ=255)                                                                        tGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACg                                                                                                           <  5:339028/94‑1 (MQ=255)                                                                        tGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCagc                                                               >  1:198164/1‑136 (MQ=255)                                                                        tGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCagc                                                               >  6:53779/1‑136 (MQ=255)                                                                        tGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCagc                                                               >  6:236364/1‑136 (MQ=255)                                                                        tGCACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCagc                                                               >  5:240584/1‑136 (MQ=255)                                                                          cACCGCTATCAGCAGATGGTTTGATTTCAAGTTTTGCCACCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTAATTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCagcaag                                                            >  8:55208/1‑134 (MQ=255)                                                                             cgctaTCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGa                                                          >  5:190185/2‑138 (MQ=255)                                                                               ctatCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGaaaa                                                       >  7:94155/4‑139 (MQ=255)                                                                               ctatCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGaaa                                                        >  4:87987/4‑138 (MQ=255)                                                                               ctatCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTATTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGaaa                                                        >  7:210199/4‑138 (MQ=255)                                                                                tatCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGaaaac                                                      >  8:399178/3‑138 (MQ=255)                                                                                    agcagATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAAcgcg                                                  >  5:221227/1‑138 (MQ=255)                                                                                    agcagATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAAcgcg                                                  >  8:249322/1‑138 (MQ=255)                                                                                        gATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCATTACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCt                                               >  7:172172/1‑138 (MQ=255)                                                                                         aTGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATCCCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTATGGAACCATCGCGCCAGCAGCAAGGAAAAAcgcgcctg                                              >  3:376258/1‑133 (MQ=255)                                                                                         aTGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACg                                                                                                           <  6:253500/77‑1 (MQ=255)                                                                                         aTGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACg                                                                                                           >  5:253500/1‑77 (MQ=255)                                                                                         aTGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTg                                              >  7:226012/1‑138 (MQ=255)                                                                                         aTGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTg                                              >  5:232666/1‑138 (MQ=255)                                                                                         aTGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTg                                              >  6:221635/1‑138 (MQ=255)                                                                                         aTGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTg                                              >  5:81503/1‑138 (MQ=255)                                                                                              ttGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGAGGCTGAAGaaa                                        >  7:115148/1‑139 (MQ=255)                                                                                                gATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATg                                      >  6:389152/1‑139 (MQ=255)                                                                                                         ttttGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGa                             <  7:234980/139‑1 (MQ=255)                                                                                                             gCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACa                          >  6:398581/1‑138 (MQ=255)                                                                                                              cccccATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACaaa                        <  6:190185/139‑1 (MQ=255)                                                                                                                   aTGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACg                                                                                                           >  1:115690/1‑51 (MQ=255)                                                                                                                   aTGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACg                                                                                                           <  2:115690/51‑1 (MQ=255)                                                                                                                           aaGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCt           >  8:3702/1‑139 (MQ=255)                                                                                                                             ggACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGt         <  6:81503/139‑1 (MQ=255)                                                                                                                             ggACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGt         <  6:232666/139‑1 (MQ=255)                                                                                                                                cATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAACCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCag      >  6:399693/1‑138 (MQ=255)                                                                                                                                cATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGTGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCag      >  4:252965/1‑138 (MQ=255)                                                                                                                                cATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCag      >  2:344634/1‑138 (MQ=255)                                                                                                                                    aataatACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTc  <  8:210199/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        GGAATGATATCTCCACCAATTTCAACCGCTTTTAGAGATGACGTTTCTATGACCAAATCACCGTAAGGCTCTGCACTGCTGTCAGCAGATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATGATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTTAATACAATCTTACTGTTTGGAACCATTGCACCCGCAATAAGGAAAAACGCCGCTGAAGAAATGTCTCCAGGAACAAAAATATCGGCAGCTGTCAATTTC  >  CP006881/2186178‑2186447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑