Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,186,399	C→G	A234A (GCG→GCC)	U712_10955 ←	3‑phosphoshikimate 1‑carboxyvinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,186,399	0	C	G	100.0%	261.0 / NA	81	A234A (GCG→GCC)	U712_10955	3‑phosphoshikimate 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (49/32);  total (49/32)

GATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATGATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTTAATACAATCTTACTGTTTGGAACCATTGCACCCGCAATAAGGAAAAACGCCGCTGAAGAAATGTCTCCAGGAACAAAAATATCGGCAGCTGTCAATTTCTGGCCGCCTGTAATAGAAACACTCGTTTGATCTTCAGAAAGCTTAACACCAAAAGCAGAAAGCATCCGCTCAGTATGGTCCCGAG  >  CP006881/2186265‑2186532                                                                                                                                       |                                                                                                                                      gATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCATTACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCt                                                                                                                                    >  7:172172/1‑138 (MQ=255)  aTGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTg                                                                                                                                   >  7:226012/1‑138 (MQ=255)  aTGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTg                                                                                                                                   >  5:232666/1‑138 (MQ=255)  aTGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTg                                                                                                                                   >  6:221635/1‑138 (MQ=255)  aTGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTg                                                                                                                                   >  5:81503/1‑138 (MQ=255)       ttGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGAGGCTGAAGaaa                                                                                                                             >  7:115148/1‑139 (MQ=255)         gATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATg                                                                                                                           >  6:389152/1‑139 (MQ=255)                  ttttGCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGa                                                                                                                  <  7:234980/139‑1 (MQ=255)                      gCCCCCATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACa                                                                                                               >  6:398581/1‑138 (MQ=255)                       cccccATGTTTTGAAGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACaaa                                                                                                             <  6:190185/139‑1 (MQ=255)                                    aaGGACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCt                                                                                                >  8:3702/1‑139 (MQ=255)                                      ggACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGt                                                                                              <  6:81503/139‑1 (MQ=255)                                      ggACATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGt                                                                                              <  6:232666/139‑1 (MQ=255)                                         cATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAACCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCag                                                                                           >  6:399693/1‑138 (MQ=255)                                         cATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGTGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCag                                                                                           >  4:252965/1‑138 (MQ=255)                                         cATCAATAATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCag                                                                                           >  2:344634/1‑138 (MQ=255)                                             aataatACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTc                                                                                       <  8:210199/139‑1 (MQ=255)                                                 aTACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAACCGCGTCTGAAGCAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTgtc                                                                                   >  2:407981/1‑137 (MQ=255)                                                  tACCTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGgcc                                                                                  >  2:7931/1‑139 (MQ=255)                                                    ccTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATTAAATTGTGCTGTTTGGAAGCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGgccac                                                                                <  4:48581/139‑3 (MQ=255)                                                    ccTGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGgccac                                                                                >  4:350111/1‑137 (MQ=255)                                                      tGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATcag                                                                                                     >  7:94922/1‑114 (MQ=255)                                                      tGTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATcag                                                                                                     <  8:94922/116‑3 (MQ=255)                                                       gTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAATGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGgccaccag                                                                             >  5:150688/1‑137 (MQ=255)                                                       gTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATcagcag                                                                                                  <  3:366138/118‑1 (MQ=255)                                                       gTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATcagcag                                                                                                  >  4:366138/1‑118 (MQ=255)                                                       gTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGgccaccag                                                                             >  1:233557/1‑137 (MQ=255)                                                       gTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGgccaccag                                                                             >  1:258846/1‑137 (MQ=255)                                                       gTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGgccaccag                                                                             >  2:132540/1‑137 (MQ=255)                                                       gTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGgccaccag                                                                             >  6:373133/1‑137 (MQ=255)                                                       gTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGgccaccag                                                                             >  7:394541/1‑137 (MQ=255)                                                       gTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGgccaccag                                                                             >  7:63393/1‑137 (MQ=255)                                                       gTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGgccaccag                                                                             >  2:364980/1‑137 (MQ=255)                                                       gTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGgccaccag                                                                             >  2:391274/1‑137 (MQ=255)                                                       gTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGgccaccag                                                                             >  3:287467/1‑137 (MQ=255)                                                       gTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGgccaccag                                                                             >  5:76825/1‑137 (MQ=255)                                                       gTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGgccaccag                                                                             >  5:368473/1‑137 (MQ=255)                                                       gTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAACAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAACTATCAGCAGCTGTCAGTTTCTGgccaccag                                                                             >  6:283855/1‑137 (MQ=255)                                                       gTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGAAGCAAGGAAAAACTCGGCTGAAGAAATGTCTCAAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGgccaccag                                                                             >  3:48581/1‑137 (MQ=255)                                                       gTCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACAATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGgccaccag                                                                             >  7:367960/1‑137 (MQ=255)                                                       gTCAGAGTCGGATTTAAACCTACGTTTTTCAATACAAAACTGCTGTTTGGAACAATCGAGCCAGCAGCAAGGAAAAAAGCGGATGAAGAAATGTCTCCATGAACAAAAATATAATCACCTGTCAGAATCTGgccaccag                                                                             >  5:213683/1‑137 (MQ=255)                                                        tCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTcc                                                                                                                      <  3:218827/97‑1 (MQ=255)                                                        tCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTcc                                                                                                                      >  4:218827/1‑97 (MQ=255)                                                        tCCGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGgccaccagc                                                                            <  1:132540/139‑4 (MQ=255)                                                         ccGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGgccaccagc                                                                            >  7:279206/1‑135 (MQ=255)                                                         ccGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGgccaccagc                                                                            >  3:399070/1‑135 (MQ=255)                                                         ccGAGTCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGgccaccagc                                                                            >  6:238443/1‑135 (MQ=255)                                                              tCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGa                                                                      <  6:368473/139‑1 (MQ=255)                                                                  aTTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAaca                                                                  <  6:76825/139‑1 (MQ=255)                                                                    ttAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTTAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACt                                                                >  6:31114/1‑139 (MQ=255)                                                                        aCCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGtt                                                            <  8:367960/139‑1 (MQ=255)                                                                          cTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTg                                                          <  8:394541/139‑1 (MQ=255)                                                                            aCGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGAt                                                        >  1:222138/1‑139 (MQ=255)                                                                            aCGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGAt                                                        >  2:365446/1‑139 (MQ=255)                                                                             cGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGGCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGAt                                                        <  8:115148/138‑1 (MQ=255)                                                                                    caaTACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGa                                                 <  3:87987/137‑1 (MQ=255)                                                                                            ttctgcTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGAt                                                        >  4:325812/6‑123 (MQ=255)                                                                                            ttctgcTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGAt                                                        <  3:325812/118‑1 (MQ=255)                                                                                            ttctgcTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAac                                        >  4:308863/6‑139 (MQ=255)                                                                                            atctgcTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAACCGCGGCTGAAGAAAGGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGACCCCAGCAATCGAAACACTCGTTTGATCTTCAGAAAGCTTAac                                        >  6:52802/6‑139 (MQ=255)                                                                                                 cTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCaa                                   <  8:94155/139‑1 (MQ=255)                                                                                                    tttGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCGAAAg                                >  5:37913/1‑139 (MQ=255)                                                                                                        gAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAg                             <  8:63393/138‑1 (MQ=255)                                                                                                         aaCCATCGCGCCAGCAGCAAGGAAAACCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGaa                           <  4:421283/139‑1 (MQ=255)                                                                                                         aaCCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGaa                           <  2:18040/139‑1 (MQ=255)                                                                                                         aaCCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGaa                           <  5:221635/139‑1 (MQ=255)                                                                                                           ccATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAg                         >  5:344217/1‑139 (MQ=255)                                                                                                           ccATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAg                         >  5:58938/1‑139 (MQ=255)                                                                                                                       cagcaaGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGt             <  5:31114/135‑1 (MQ=255)                                                                                                                        agcaaGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGtg            <  4:65250/136‑2 (MQ=255)                                                                                                                        agcaaGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGtg            <  2:119616/136‑2 (MQ=255)                                                                                                                          caaGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGtgtg          <  3:351206/138‑1 (MQ=255)                                                                                                                               aaaaaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTccc     <  6:58938/139‑1 (MQ=255)                                                                                                                               aaaaaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTccc     <  6:344217/139‑1 (MQ=255)                                                                                                                               aaaaaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTccc     <  3:308073/139‑1 (MQ=255)                                                                                                                               aaaaaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTccc     >  2:168839/1‑139 (MQ=255)                                                                                                                               aaaaaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTccc     <  1:7931/139‑1 (MQ=255)                                                                                                                                aaaaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTccc     <  4:287467/138‑1 (MQ=255)                                                                                                                                  aaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTCACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCgag  <  2:258846/139‑1 (MQ=255)                                                                                                                                  aaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTccc     >  6:292325/1‑136 (MQ=255)                                                                                                                                  aaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCgag  >  1:160888/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                      GATGGTTTGATTTCAAGTTTTGCCCCCATGTTTTGAAGGACATCAATGATACCTGTCCGAGTCGGATTTAAACCTACGTTTTTTAATACAATCTTACTGTTTGGAACCATTGCACCCGCAATAAGGAAAAACGCCGCTGAAGAAATGTCTCCAGGAACAAAAATATCGGCAGCTGTCAATTTCTGGCCGCCTGTAATAGAAACACTCGTTTGATCTTCAGAAAGCTTAACACCAAAAGCAGAAAGCATCCGCTCAGTATGGTCCCGAG  >  CP006881/2186265‑2186532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑