Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,186,456	T→A	T215A (ACA→GCT)	U712_10955 ←	3‑phosphoshikimate 1‑carboxyvinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,186,456	0	T	A	100.0%	207.7 / NA	65	T215A (ACA→GCT)	U712_10955	3‑phosphoshikimate 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (18/47);  total (18/47)

TCGGATTTAAACCTACGTTTTTTAATACAATCTTACTGTTTGGAACCATTGCACCCGCAATAAGGAAAAACGCCGCTGAAGAAATGTCTCCAGGAACAAAAATATCGGCAGCTGTCAATTTCTGGCCGCCTGTAATAGAAACACTCGTTTGATCTTCAGAAAGCTTAACACCAAAAGCAGAAAGCATCCGCTCAGTATGGTCCCGAGATTTATGAGGCTCTGTGACGGTAGTTGTGCCCTCAGCCTGTAATCCAGCCAG  >  CP006881/2186326‑2186584                                                                                                                                   |                                                                                                                                 tCGGATTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGa                                                                                                                          <  6:368473/139‑1 (MQ=255)     aTTTAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAaca                                                                                                                      <  6:76825/139‑1 (MQ=255)       ttAAACCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTTAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACt                                                                                                                    >  6:31114/1‑139 (MQ=255)           aCCTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGtt                                                                                                                <  8:367960/139‑1 (MQ=255)             cTACGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTg                                                                                                              <  8:394541/139‑1 (MQ=255)               aCGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGAt                                                                                                            >  1:222138/1‑139 (MQ=255)               aCGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGAt                                                                                                            >  2:365446/1‑139 (MQ=255)                cGTTTTTCAATACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGGCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGAt                                                                                                            <  8:115148/138‑1 (MQ=255)                       caaTACAATTCTGCTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGa                                                                                                     <  3:87987/137‑1 (MQ=255)                               ttctgcTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGAt                                                                                                            >  4:325812/6‑123 (MQ=255)                               ttctgcTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGAt                                                                                                            <  3:325812/118‑1 (MQ=255)                               ttctgcTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAac                                                                                            >  4:308863/6‑139 (MQ=255)                               atctgcTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAACCGCGGCTGAAGAAAGGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGACCCCAGCAATCGAAACACTCGTTTGATCTTCAGAAAGCTTAac                                                                                            >  6:52802/6‑139 (MQ=255)                                    cTGTTTGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCaa                                                                                       <  8:94155/139‑1 (MQ=255)                                       tttGGAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCGAAAg                                                                                    >  5:37913/1‑139 (MQ=255)                                           gAACCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAg                                                                                 <  8:63393/138‑1 (MQ=255)                                            aaCCATCGCGCCAGCAGCAAGGAAAACCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGaa                                                                               <  4:421283/139‑1 (MQ=255)                                            aaCCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGaa                                                                               <  5:221635/139‑1 (MQ=255)                                            aaCCATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGaa                                                                               <  2:18040/139‑1 (MQ=255)                                              ccATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAg                                                                             >  5:58938/1‑139 (MQ=255)                                              ccATCGCGCCAGCAGCAAGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAg                                                                             >  5:344217/1‑139 (MQ=255)                                                          cagcaaGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGt                                                                 <  5:31114/135‑1 (MQ=255)                                                           agcaaGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGtg                                                                <  4:65250/136‑2 (MQ=255)                                                           agcaaGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGtg                                                                <  2:119616/136‑2 (MQ=255)                                                             caaGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGtgtg                                                              <  3:351206/138‑1 (MQ=255)                                                                  aaaaaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTccc                                                         <  6:344217/139‑1 (MQ=255)                                                                  aaaaaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTccc                                                         <  1:7931/139‑1 (MQ=255)                                                                  aaaaaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTccc                                                         >  2:168839/1‑139 (MQ=255)                                                                  aaaaaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTccc                                                         <  6:58938/139‑1 (MQ=255)                                                                  aaaaaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTccc                                                         <  3:308073/139‑1 (MQ=255)                                                                   aaaaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTccc                                                         <  4:287467/138‑1 (MQ=255)                                                                     aaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTCACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCgag                                                      <  2:258846/139‑1 (MQ=255)                                                                     aaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTccc                                                         >  6:292325/1‑136 (MQ=255)                                                                     aaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCgag                                                      >  1:160888/1‑139 (MQ=255)                                                                               cagaaCTGTCTCGAGTAACAAAAATATCAGCAGCTGGCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTACCGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATgggg                                            <  4:376258/138‑1 (MQ=255)                                                                               aagaaATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATgggg                                            <  4:399070/139‑1 (MQ=255)                                                                               aagaaATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATgggg                                            <  5:238443/139‑1 (MQ=255)                                                                               aagaaATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATgggg                                            <  2:160888/139‑1 (MQ=255)                                                                               aagaaATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATgggg                                            <  3:350111/139‑1 (MQ=255)                                                                               aagaaATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATgggg                                            <  1:391274/139‑1 (MQ=255)                                                                               aagaaATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATgggg                                            <  5:399693/139‑1 (MQ=255)                                                                               aagaaATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATgggg                                            <  3:244137/139‑1 (MQ=255)                                                                               aagaaATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTGCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGGCCCGAGATTTATgggg                                            <  6:221227/139‑1 (MQ=255)                                                                                agaaATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATgggg                                            <  1:365446/138‑1 (MQ=255)                                                                                 gaaATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGct                                          <  3:308863/139‑1 (MQ=255)                                                                                 gaaATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGct                                          <  3:252965/139‑1 (MQ=255)                                                                                    aTGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGtg                                                                >  5:181428/1‑113 (MQ=255)                                                                                    aTGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGtg                                                                <  6:181428/114‑2 (MQ=255)                                                                                     tGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCtgt                                      <  5:292325/139‑1 (MQ=255)                                                                                      gTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCtgtt                                     >  2:219696/1‑138 (MQ=255)                                                                                         tcCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCtgtta                                    >  4:435707/1‑135 (MQ=255)                                                                                           cAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTAcag                                 <  2:233557/138‑3 (MQ=255)                                                                                           cAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTAca                                  <  7:399178/137‑2 (MQ=255)                                                                                           cAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTAc                                   <  3:101526/136‑1 (MQ=255)                                                                                           cAGGAACAAAAATATCAGCAGCTGTCAGGTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTAcag                                 <  1:364980/138‑3 (MQ=255)                                                                                              gAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAATCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGt                             <  6:150688/139‑1 (MQ=255)                                                                                                aCAAAAATATCAGCAGCGGGCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGACAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGCGATTTATGGGGCTCTGTTACAGttgttg                           <  6:240584/139‑1 (MQ=255)                                                                                                 cAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCa                                                                           >  4:345300/1‑90 (MQ=255)                                                                                                 cAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCa                                                                           <  3:345300/90‑1 (MQ=255)                                                                                                  aaaaaTATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTtgtg                         <  1:407981/139‑1 (MQ=255)                                                                                                   aaaaTATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGc                        >  8:399144/1‑139 (MQ=255)                                                                                                      atatCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCt                     <  5:53779/139‑1 (MQ=255)                                                                                                       tatCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTc                    <  6:79564/139‑1 (MQ=255)                                                                                                       tatCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTc                    <  1:344634/139‑1 (MQ=255)                                                                                                                          cTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATccggccag  >  3:428099/1‑138 (MQ=255)                                                                                                                                   |                                                                                                                                 TCGGATTTAAACCTACGTTTTTTAATACAATCTTACTGTTTGGAACCATTGCACCCGCAATAAGGAAAAACGCCGCTGAAGAAATGTCTCCAGGAACAAAAATATCGGCAGCTGTCAATTTCTGGCCGCCTGTAATAGAAACACTCGTTTGATCTTCAGAAAGCTTAACACCAAAAGCAGAAAGCATCCGCTCAGTATGGTCCCGAGATTTATGAGGCTCTGTGACGGTAGTTGTGCCCTCAGCCTGTAATCCAGCCAG  >  CP006881/2186326‑2186584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑