Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,186,522	A→G	H193H (CAT→CAC)	U712_10955 ←	3‑phosphoshikimate 1‑carboxyvinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,186,522	0	A	G	100.0%	193.5 / NA	60	H193H (CAT→CAC)	U712_10955	3‑phosphoshikimate 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/43);  total (17/43)

TAAGGAAAAACGCCGCTGAAGAAATGTCTCCAGGAACAAAAATATCGGCAGCTGTCAATTTCTGGCCGCCTGTAATAGAAACACTCGTTTGATCTTCAGAAAGCTTAACACCAAAAGCAGAAAGCATCCGCTCAGTATGGTCCCGAGATTTATGAGGCTCTGTGACGGTAGTTGTGCCCTCAGCCTGTAATCCAGCCAGCAAAACAGCAGATTTAATTTGTGCGCTTGCAACAGGTGATACATAATCAATGCCTTTTAATGAAGCGCCG  >  CP006881/2186386‑2186654                                                                                                                                         |                                                                                                                                     caaGGAAAAACGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGtgtg                                                                                                                                    <  3:351206/138‑1 (MQ=255)      aaaaaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTccc                                                                                                                               <  6:58938/139‑1 (MQ=255)      aaaaaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTccc                                                                                                                               <  3:308073/139‑1 (MQ=255)      aaaaaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTccc                                                                                                                               <  6:344217/139‑1 (MQ=255)      aaaaaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTccc                                                                                                                               <  1:7931/139‑1 (MQ=255)      aaaaaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTccc                                                                                                                               >  2:168839/1‑139 (MQ=255)       aaaaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTccc                                                                                                                               <  4:287467/138‑1 (MQ=255)         aaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTCACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCgag                                                                                                                            <  2:258846/139‑1 (MQ=255)         aaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTccc                                                                                                                               >  6:292325/1‑136 (MQ=255)         aaCGCGGCTGAAGAAATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCgag                                                                                                                            >  1:160888/1‑139 (MQ=255)                   cagaaCTGTCTCGAGTAACAAAAATATCAGCAGCTGGCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTACCGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATgggg                                                                                                                  <  4:376258/138‑1 (MQ=255)                   aagaaATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATgggg                                                                                                                  <  3:244137/139‑1 (MQ=255)                   aagaaATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATgggg                                                                                                                  <  3:350111/139‑1 (MQ=255)                   aagaaATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATgggg                                                                                                                  <  2:160888/139‑1 (MQ=255)                   aagaaATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATgggg                                                                                                                  <  4:399070/139‑1 (MQ=255)                   aagaaATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATgggg                                                                                                                  <  1:391274/139‑1 (MQ=255)                   aagaaATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATgggg                                                                                                                  <  5:238443/139‑1 (MQ=255)                   aagaaATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATgggg                                                                                                                  <  5:399693/139‑1 (MQ=255)                   aagaaATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTGCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGGCCCGAGATTTATgggg                                                                                                                  <  6:221227/139‑1 (MQ=255)                    agaaATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATgggg                                                                                                                  <  1:365446/138‑1 (MQ=255)                     gaaATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGct                                                                                                                <  3:252965/139‑1 (MQ=255)                     gaaATGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGct                                                                                                                <  3:308863/139‑1 (MQ=255)                         tGTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCtgt                                                                                                            <  5:292325/139‑1 (MQ=255)                          gTCTCCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCtgtt                                                                                                           >  2:219696/1‑138 (MQ=255)                             tcCAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCtgtta                                                                                                          >  4:435707/1‑135 (MQ=255)                               cAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTAcag                                                                                                       <  2:233557/138‑3 (MQ=255)                               cAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTAca                                                                                                        <  7:399178/137‑2 (MQ=255)                               cAGGAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTAc                                                                                                         <  3:101526/136‑1 (MQ=255)                               cAGGAACAAAAATATCAGCAGCTGTCAGGTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTAcag                                                                                                       <  1:364980/138‑3 (MQ=255)                                  gAACAAAAATATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAATCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGt                                                                                                   <  6:150688/139‑1 (MQ=255)                                    aCAAAAATATCAGCAGCGGGCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGACAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGCGATTTATGGGGCTCTGTTACAGttgttg                                                                                                 <  6:240584/139‑1 (MQ=255)                                      aaaaaTATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTtgtg                                                                                               <  1:407981/139‑1 (MQ=255)                                       aaaaTATCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGc                                                                                              >  8:399144/1‑139 (MQ=255)                                          atatCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCt                                                                                           <  5:53779/139‑1 (MQ=255)                                           tatCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTc                                                                                          <  6:79564/139‑1 (MQ=255)                                           tatCAGCAGCTGTCAGTTTCTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTc                                                                                          <  1:344634/139‑1 (MQ=255)                                                              cTGGCCACCAGCAATGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATccggccag                                                                        >  3:428099/1‑138 (MQ=255)                                                                      cagcaaTGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAAcag                                                                >  1:45766/5‑138 (MQ=255)                                                                      cagcaaTGGAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAAcag                                                                >  1:52491/5‑138 (MQ=255)                                                                           atggAAACACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGAtt                                                          >  2:15773/4‑139 (MQ=255)                                                                                aaCACTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAAtt                                                     >  4:49871/1‑139 (MQ=255)                                                                                    cTCGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTtgcg                                                 >  4:202960/1‑137 (MQ=255)                                                                                      cGTTTGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGcgcg                                               >  5:260975/1‑139 (MQ=255)                                                                                        tttGATCTTCAGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGCGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCt                                             <  4:428099/139‑1 (MQ=255)                                                                                                 cAGAAAGCTTAACGCCAAAATCAGAAAGCAGCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAAc                                       <  5:52802/136‑1 (MQ=255)                                                                                                  aGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGt                                   <  5:120235/139‑1 (MQ=255)                                                                                                  aGAAAGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGt                                   <  5:283855/139‑1 (MQ=255)                                                                                                      aGCTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATa                               >  5:292804/1‑139 (MQ=255)                                                                                                        cTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACa                             <  1:168839/139‑1 (MQ=255)                                                                                                        cTTAACGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACa                             <  3:202960/139‑1 (MQ=255)                                                                                                           aaCGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCtgtta                                                                                                          >  7:340854/1‑57 (MQ=255)                                                                                                           aaCGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCtgtta                                                                                                          <  8:340854/59‑3 (MQ=255)                                                                                                           aaCGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACa                             >  4:77700/1‑136 (MQ=255)                                                                                                           aaCGCCAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACa                             <  3:77700/136‑1 (MQ=255)                                                                                                               ccAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCaa                      <  2:52491/139‑1 (MQ=255)                                                                                                               ccAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCaa                      <  2:45766/139‑1 (MQ=255)                                                                                                               ccAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCaa                      <  2:222138/139‑1 (MQ=255)                                                                                                                cAAAAGCAGAAAGCATCCGCTCAGTGTGGTCCCGAGATCTATGGGGCGCTGTGACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAAt                     <  8:251271/139‑1 (MQ=255)                                                                                                                      cAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTGGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCttt               <  6:260975/139‑1 (MQ=255)                                                                                                                                   cTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCACCAGGTGATACATAATCAATTCCTTTTAATGAAGcgccg  >  3:429831/1‑139 (MQ=255)                                                                                                                                   cTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGcgccg  <  3:49871/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     TAAGGAAAAACGCCGCTGAAGAAATGTCTCCAGGAACAAAAATATCGGCAGCTGTCAATTTCTGGCCGCCTGTAATAGAAACACTCGTTTGATCTTCAGAAAGCTTAACACCAAAAGCAGAAAGCATCCGCTCAGTATGGTCCCGAGATTTATGAGGCTCTGTGACGGTAGTTGTGCCCTCAGCCTGTAATCCAGCCAGCAAAACAGCAGATTTAATTTGTGCGCTTGCAACAGGTGATACATAATCAATGCCTTTTAATGAAGCGCCG  >  CP006881/2186386‑2186654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑