Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,173,022	G→A	D242D (GAC→GAT)	U712_10870 ←	Bifunctional protein BirA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,173,022	0	G	A	100.0%	254.6 / NA	82	D242D (GAC→GAT)	U712_10870	Bifunctional protein BirA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/38);  total (44/38)

TGGCTCTCATATTAGTGCCGATTCCCAACGCATAACTTTCCCATAAAAGCTTAATCGGCGTAAAACCGTGTGTCATATAATCCCGGTACCGTTTTTCAAAGCAAAGTAAAATATGCTGGATGACGCCGGCCCGGTCAATTTTTTCTCCGGAAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAGTTCATCTGGAAAATCATCAGACTGCTGGTTAACGTTAATGCCGATCCCGATGATAACTGACCGTACGCGGTCTTCCTCGGCCT  >  CP006881/2172889‑2173155                                                                                                                                      |                                                                                                                                      tGGCTCTCATATTAGTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAAt                                                                                                                                  >  6:154335/1‑139 (MQ=255)               gTGCCAATACCGAGCGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTccccagcag                                                                                                                    <  5:110596/139‑6 (MQ=255)                        ccgagcGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCt                                                                                                             >  7:317489/6‑137 (MQ=255)                          gagcGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAgg                                                                                                         >  3:67994/4‑139 (MQ=255)                          gagcGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAgg                                                                                                         >  5:73119/4‑139 (MQ=255)                          gagcGCATAGCTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAgg                                                                                                         >  3:360404/4‑139 (MQ=255)                                   gcTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTcgcg                                                                                                <  2:365564/138‑1 (MQ=255)                                    cTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGa                                                                                               <  2:116984/139‑1 (MQ=255)                                    cTTTCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGa                                                                                               <  2:46896/139‑1 (MQ=255)                                       tCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGCGGCTTGTCGCGAGGt                                                                                            >  8:205954/1‑139 (MQ=255)                                       tCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGAt                                                                                              <  7:380489/137‑1 (MQ=255)                                       tCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGAt                                                                                              <  5:172304/137‑1 (MQ=255)                                       tCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGt                                                                                            >  2:347003/1‑139 (MQ=255)                                       tCCCATAAAAGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGt                                                                                            >  7:185751/1‑139 (MQ=255)                                              aaaGCTTAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCaa                                                                                     >  1:380279/1‑138 (MQ=255)                                                    tAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCagcagc                                                                                                                   <  5:276901/103‑1 (MQ=255)                                                    tAATCGGCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCagcagc                                                                                                                   >  6:276901/1‑103 (MQ=255)                                                         gtcGTAAATCCGTGCGTCATATAATGCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGaa                                                                          <  2:417152/137‑1 (MQ=255)                                                         ggCGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGaa                                                                          <  3:212416/139‑1 (MQ=255)                                                           cGTAAATCCGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCCGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCTTCTGGaaaa                                                                        >  7:256821/1‑139 (MQ=255)                                                               aatccGTGCGTCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAatca                                                                     <  6:286049/135‑1 (MQ=255)                                                                    gtgcgtCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGAGGTCCTTCAATTCATCTGGAAAATCATTAggc                                                               >  1:260607/1‑137 (MQ=255)                                                                     tgcgtCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCagcagc                                                                                                                   <  5:46542/83‑1 (MQ=255)                                                                     tgcgtCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCagcagc                                                                                                                   >  6:46542/4‑86 (MQ=255)                                                                     tgcgtCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATg                                                                                             <  7:146512/105‑1 (MQ=255)                                                                     tgcgtCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATg                                                                                             >  8:146512/4‑108 (MQ=255)                                                                     tgcgtCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGct                                                              >  3:18886/4‑139 (MQ=255)                                                                     tgcgtCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGct                                                              >  6:315200/4‑139 (MQ=255)                                                                     tgcgtCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGct                                                              >  4:322248/4‑139 (MQ=255)                                                                     tgcgtCATATAATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGCATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTCGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGct                                                              >  8:42391/4‑139 (MQ=255)                                                                            tataATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGGTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGtt                                                       <  2:380279/139‑1 (MQ=255)                                                                            tataATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGtt                                                       <  2:332920/139‑1 (MQ=255)                                                                            tataATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGtt                                                       <  5:315200/139‑1 (MQ=255)                                                                            tataATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGtt                                                       <  5:255653/139‑1 (MQ=255)                                                                            tataATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGtt                                                       <  4:67994/139‑1 (MQ=255)                                                                            tataATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGtt                                                       <  3:387886/139‑1 (MQ=255)                                                                            tataATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGtt                                                       <  5:154335/139‑1 (MQ=255)                                                                            tataATCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGAGGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGtt                                                       <  6:201560/139‑1 (MQ=255)                                                                                 tCCCGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGt                                                  >  4:184080/1‑139 (MQ=255)                                                                                   ccGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGtt                                                 >  8:420153/1‑138 (MQ=255)                                                                                    cGGTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTGCTTCAAGTCATCTGGAAAATCAGTAGTCTGCTGGTTAACGTTaa                                               >  6:127712/1‑139 (MQ=255)                                                                                      gTACCGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATg                                             >  7:422467/1‑139 (MQ=255)                                                                                         ccGTTTCTCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGcc                                           <  1:347003/138‑1 (MQ=255)                                                                                            tttctCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGaa                                                                          >  4:285749/1‑104 (MQ=255)                                                                                            tttctCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGaa                                                                          <  3:285749/104‑1 (MQ=255)                                                                                              tctCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGcc                                           <  4:119444/131‑1 (MQ=255)                                                                                              tctCAAAGCATAGTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGcc                                           >  3:119444/3‑133 (MQ=255)                                                                                               ctCAAAGCATAGTAAAATATGCTGGATGACGCCGGCACGATCAATTTTTTCCCCAGCAGCTTGGCGGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATccc                                     <  5:442130/137‑1 (MQ=255)                                                                                                  aaagcatagTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCaat                                  <  4:18886/138‑1 (MQ=255)                                                                                                   aagcatagTAAAATATGCTGGATGACGCCTGCCCGATCTATTTTTTCACCAGCAGATTTGCTGATGATTGTCGCGATGTCCTTCATTTCAAATGGAAAATCATTATGCTGCTGGTTAACGTTAATGCCGATCCCaatga                                <  6:227804/139‑1 (MQ=255)                                                                                                      catagTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCaatgatca                             <  8:185751/136‑3 (MQ=255)                                                                                                         agTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCt                                                                                                             <  1:278090/56‑1 (MQ=255)                                                                                                         agTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCt                                                                                                             >  2:278090/1‑56 (MQ=255)                                                                                                         agTAAAATATGCTGGATGACGCCGGCCCGATCAATTTTTTCCCAAGCAGCTTGGCTGAGTCTTGTAGCGATGTCCTTAAATTAAAATTGAAAATCATTAGGATGCTTGTTAACGTTAATGCAGATCACAATGATCACt                           >  5:227804/1‑138 (MQ=255)                                                                                                                tatGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCt                                                                                                             <  5:295413/49‑1 (MQ=255)                                                                                                                tatGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCt                                                                                                             >  6:295413/1‑49 (MQ=255)                                                                                                                tatGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGtt                                                       >  8:378070/1‑103 (MQ=255)                                                                                                                tatGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGtt                                                       <  7:378070/103‑1 (MQ=255)                                                                                                                tatGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTAc                   >  5:403864/1‑139 (MQ=255)                                                                                                                tatGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTAc                   >  1:54923/1‑139 (MQ=255)                                                                                                                  tGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTAcg                  <  3:82889/138‑1 (MQ=255)                                                                                                                  tGCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTAcg                  <  5:146907/138‑1 (MQ=255)                                                                                                                   gCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTAcgcg                >  5:296281/1‑139 (MQ=255)                                                                                                                   gCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTAcgcg                >  1:36398/1‑139 (MQ=255)                                                                                                                   gCTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTAcgcg                >  3:187159/1‑139 (MQ=255)                                                                                                                   gCTGGATGACGCCGGCCCGATCAATTTTTTCCCCACCAGCTTGGCTGAGGCTTGTCGCGATTTCCTTCAATTCATCTGGAAAAACATTAGGCTGCTGGTTAACGTTAAGGCCGATCCCAATGATCACTTAACGGAcgcg                >  4:82889/1‑139 (MQ=255)                                                                                                                    cTGGATGACGCCGGCCCTATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTAcgcg                <  1:256207/138‑1 (MQ=255)                                                                                                                    cTGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTAcgcg                <  4:360404/138‑1 (MQ=255)                                                                                                                     tGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCt                                                                                                             >  4:444979/1‑44 (MQ=255)                                                                                                                     tGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGt              >  5:81155/1‑139 (MQ=255)                                                                                                                     tGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGt              >  3:217751/1‑139 (MQ=255)                                                                                                                     tGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGt              >  3:262514/1‑139 (MQ=255)                                                                                                                     tGGATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTCAGGCTTGTCGCGATGACCTTCAAATCATCTGGAAAATCATTAGTCTGCTGGTTAACGTTAATGCCGATGCCAATTATCACTGAACGTACGCGGt              >  5:145175/1‑139 (MQ=255)                                                                                                                       gATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGt              <  8:422467/137‑1 (MQ=255)                                                                                                                       gATGACGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCGGGTTACCGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCt            >  6:148001/1‑139 (MQ=255)                                                                                                                           aCGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTctt        <  3:184080/139‑3 (MQ=255)                                                                                                                            cGCCGGCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCttcttc       >  1:4726/1‑139 (MQ=255)                                                                                                                                 gCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGaa                                                                          >  7:105808/1‑67 (MQ=255)                                                                                                                                 gCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGaa                                                                          <  8:105808/67‑1 (MQ=255)                                                                                                                                 gCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGcc                                           >  3:155910/1‑98 (MQ=255)                                                                                                                                 gCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGcc                                           <  4:155910/98‑1 (MQ=255)                                                                                                                                 gCCCGATCAATTTTTTCCCCAGCAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTGCTTCCGCCt  >  4:413945/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                      TGGCTCTCATATTAGTGCCGATTCCCAACGCATAACTTTCCCATAAAAGCTTAATCGGCGTAAAACCGTGTGTCATATAATCCCGGTACCGTTTTTCAAAGCAAAGTAAAATATGCTGGATGACGCCGGCCCGGTCAATTTTTTCTCCGGAAGCTTGGCTGAGGCTTGTCGCGATGTCCTTCAGTTCATCTGGAAAATCATCAGACTGCTGGTTAACGTTAATGCCGATCCCGATGATAACTGACCGTACGCGGTCTTCCTCGGCCT  >  CP006881/2172889‑2173155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑