Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,186,636	G→T	G155G (GGC→GGA)	U712_10955 ←	3‑phosphoshikimate 1‑carboxyvinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,186,636	0	G	T	100.0%	171.1 / NA	55	G155G (GGC→GGA)	U712_10955	3‑phosphoshikimate 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (32/23);  total (32/23)

CAGAAAGCATCCGCTCAGTATGGTCCCGAGATTTATGAGGCTCTGTGACGGTAGTTGTGCCCTCAGCCTGTAATCCAGCCAGCAAAACAGCAGATTTAATTTGTGCGCTTGCAACAGGTGATACATAATCAATGCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAATTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTCCTTCAAAGGCTCAGTTACACGTTTCATTGGGCGCTTCGCAATGCTCTCATCTCCGG  >  CP006881/2186503‑2186769                                                                                                                                      |                                                                                                                                      cAGAAAGCATCCGCTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTGGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCttt                                                                                                                                  <  6:260975/139‑1 (MQ=255)              cTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCACCAGGTGATACATAATCAATTCCTTTTAATGAAGcgccg                                                                                                                     >  3:429831/1‑139 (MQ=255)              cTCAGTGTGGTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGcgccg                                                                                                                     <  3:49871/139‑1 (MQ=255)                       gTCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGAc                                                                                                            >  2:42244/1‑139 (MQ=255)                        tCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGa                                                                                                             >  5:363474/1‑137 (MQ=255)                        tCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGa                                                                                                             >  3:359983/1‑137 (MQ=255)                        tCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGa                                                                                                             >  3:222452/1‑137 (MQ=255)                        tCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGa                                                                                                             >  7:227541/1‑137 (MQ=255)                        tCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACa                                                                                                           <  8:227541/139‑1 (MQ=255)                        tCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACa                                                                                                           <  7:332967/139‑1 (MQ=255)                        tCCCGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGAGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAATCGCCGCTCACTGa                                                                                                             >  1:225471/1‑137 (MQ=255)                          ccgctaTTTAGGGGGCGCGGTTACAGTGGTGGGGCCCGCCGCCGGTAAGCCGCCCACAAAAACACCATATTTAATTTGCGCGCTTGAAACAGGTTAAACAAAATAAATTCCTTTAAATCACGCGCCGCTCACTGACAGc                                                                                                         <  2:225471/134‑1 (MQ=255)                          ccGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGc                                                                                                         <  3:435707/139‑1 (MQ=255)                          ccGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGc                                                                                                         <  6:363474/139‑1 (MQ=255)                          ccGAGATTTATGGGGCTCTGTTACAGTTGTTGTGCCCGCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGc                                                                                                         <  7:399144/139‑1 (MQ=255)                             agaTTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGcgccg                                                                                                                     <  3:15099/124‑1 (MQ=255)                             agaTTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGcgccg                                                                                                                     >  4:15099/1‑124 (MQ=255)                             agaTTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGgt                                                                                                      >  3:288169/1‑139 (MQ=255)                             agaTTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGgt                                                                                                      >  8:75341/1‑139 (MQ=255)                               aTTTATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGgtg                                                                                                     >  5:78998/1‑138 (MQ=255)                                tttATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTa                                                                                                   <  1:15773/139‑1 (MQ=255)                                 ttATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTa                                                                                                   >  2:87520/1‑138 (MQ=255)                                  tATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAg                                                                                                          >  1:317153/1‑130 (MQ=255)                                  tATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTaaa                                                                                                 >  8:369624/1‑139 (MQ=255)                                  tATGGGGCTCTGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGAAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCATTTTAATGAAGCGCCGCTCACTGACAg                                                                                                          <  2:317153/130‑1 (MQ=255)                                         ctctGTTACAGTTGTTGTGCCCTCCGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCATCAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTccg                                                                                          >  1:44219/1‑139 (MQ=255)                                         ctctGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTccg                                                                                          >  8:50440/1‑139 (MQ=255)                                         ctctGTTACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTccg                                                                                          >  3:314213/1‑139 (MQ=255)                                            tgttACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAg                                                                                                          <  1:209888/120‑1 (MQ=255)                                            tgttACAGTTGTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAg                                                                                                          >  2:209888/1‑120 (MQ=255)                                                acagttgTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGTct                                                                                    <  4:288169/132‑1 (MQ=255)                                                      gtggtgCCCGCAGCCTGTAATCCGGCCATCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATTCATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGt                                                                             <  6:292804/136‑1 (MQ=255)                                                      gTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCACTTCCTTTTAATGAAGCGCCGCTCTCTGACCGCGGTGTAGACTCTCCGCCGGCTCTGCCGt                                                                             >  2:114363/1‑139 (MQ=255)                                                      gTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGt                                                                             >  4:401969/1‑139 (MQ=255)                                                      gTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGt                                                                             >  5:342279/1‑139 (MQ=255)                                                      gTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGt                                                                             >  7:48613/1‑139 (MQ=255)                                                      gTTGTGCCCTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGt                                                                             >  3:264495/1‑139 (MQ=255)                                                           gCCCCCAGCCTGAAAACCGGCCAGCAAAACAGCAGATTTAATTTCCGCGCTGGCAACAGGGCATACAAAATCCATTCCTATTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGAtt                                                                        <  7:104782/139‑1 (MQ=255)                                                              cTCAGCCTGTAATCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATATTa                                                                     >  1:73258/1‑139 (MQ=255)                                                                         aTCCGGCCAGCAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCAtttt                                                          >  4:190346/1‑138 (MQ=255)                                                                           ccggccagcAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCAtttttt                                                        >  3:362642/4‑136 (MQ=255)                                                                             ggccagcAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCAttttttt                                                       >  2:114788/2‑134 (MQ=255)                                                                             ggccagcAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCAttttttt                                                       >  4:426648/2‑134 (MQ=255)                                                                              gccagcAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCa                                                     <  1:87520/139‑1 (MQ=255)                                                                                cagcAAAACAGCAGATTTAATTTGCGCGCTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCaaa                                                   <  7:75341/139‑1 (MQ=255)                                                                                               tttAATTTGCGCGCTTGCAACAGGTGAAACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACg                                     <  1:371109/138‑1 (MQ=255)                                                                                                        cgcgcTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTggg                           >  8:52876/2‑139 (MQ=255)                                                                                                        cgcgcTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATGTTAGCCCCCATTTTTTTCAAAGGCTCCGTCACCCGGTTCATTggg                           >  8:226707/2‑139 (MQ=255)                                                                                                          cgcTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTGGgcg                         >  8:393454/1‑139 (MQ=255)                                                                                                                cAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTGGGCGTTTCg                    <  4:362642/138‑1 (MQ=255)                                                                                                                 aaCAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTGGGCGTTTCGCa                  <  8:48613/139‑1 (MQ=255)                                                                                                                   cAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGGGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTGGGCGTTTCGCAAt                <  1:42244/139‑1 (MQ=255)                                                                                                                         aTACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTGGGCGTTTCGCAATGctct           <  7:393454/138‑1 (MQ=255)                                                                                                                          tACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTGGGCGTTTCGCAATGCTCTCa         <  6:342279/139‑1 (MQ=255)                                                                                                                                 tCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTGGGCGTTTCGCAATGCTCTCATCTCCgg  >  6:388584/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                      CAGAAAGCATCCGCTCAGTATGGTCCCGAGATTTATGAGGCTCTGTGACGGTAGTTGTGCCCTCAGCCTGTAATCCAGCCAGCAAAACAGCAGATTTAATTTGTGCGCTTGCAACAGGTGATACATAATCAATGCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAATTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTCCTTCAAAGGCTCAGTTACACGTTTCATTGGGCGCTTCGCAATGCTCTCATCTCCGG  >  CP006881/2186503‑2186769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑