Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,186,729 T→C V124V (GTA→GTG)  U712_10955 ← 3‑phosphoshikimate 1‑carboxyvinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,186,7290TC100.0% 114.2 / NA 39V124V (GTA→GTG) U712_109553‑phosphoshikimate 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/23);  total (16/23)

TTTAATTTGTGCGCTTGCAACAGGTGATACATAATCAATGCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAATTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTCCTTCAAAGGCTCAGTTACACGTTTCATTGGGCGCTTCGCAATGCTCTCATCTCCGGCTACCGCGCTGTAAAAAGGACGGCCCGCCAAAATGCCGAGCATGAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGG  >  CP006881/2186597‑2186860
                                                                                                                                    |                                                                                                                                   
tttAATTTGCGCGCTTGCAACAGGTGAAACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACg                                                                                                                                <  1:371109/138‑1 (MQ=255)
         cgcgcTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTggg                                                                                                                      >  8:52876/2‑139 (MQ=255)
         cgcgcTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATGTTAGCCCCCATTTTTTTCAAAGGCTCCGTCACCCGGTTCATTggg                                                                                                                      >  8:226707/2‑139 (MQ=255)
           cgcTTGCAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTGGgcg                                                                                                                    >  8:393454/1‑139 (MQ=255)
                 cAACAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTGGGCGTTTCg                                                                                                               <  4:362642/138‑1 (MQ=255)
                  aaCAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTGGGCGTTTCGCa                                                                                                             <  8:48613/139‑1 (MQ=255)
                    cAGGTGATACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGGGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTGGGCGTTTCGCAAt                                                                                                           <  1:42244/139‑1 (MQ=255)
                          aTACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTGGGCGTTTCGCAATGctct                                                                                                      <  7:393454/138‑1 (MQ=255)
                           tACATAATCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTGGGCGTTTCGCAATGCTCTCa                                                                                                    <  6:342279/139‑1 (MQ=255)
                                  tCAATTCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTGGGCGTTTCGCAATGCTCTCATCTCCgg                                                                                             >  6:388584/1‑139 (MQ=255)
                                     attccTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGAGTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACCCGCTTCATTGGGCGTTTCGCAATGCTCTCATCTCCGGCTa                                                                                          >  2:75456/4‑139 (MQ=255)
                                      ttccTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCCCACGCGTCATTGGGCGGTTAGCAATGCTAGAATCTCCGGCTAc                                                                                         >  8:191969/3‑139 (MQ=255)
                                      ttccTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACCCGCTTAATTGGGCCGTTCGCAATGCTCTCATGTCCGGCTAc                                                                                         >  8:348305/3‑139 (MQ=255)
                                      ttccTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTGGGCGTTTCGCAATGCTCTCATCTCCGGCTAc                                                                                         >  5:399061/3‑139 (MQ=255)
                                      ttccTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTAAAAGGCTCAGTCACACGCTTCATTGGGCGTTTCACAATGCTCTCATCTCCGGCTAc                                                                                         >  3:151129/3‑139 (MQ=255)
                                            ttAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTGAAAGGGTCAGTCACACGCGTCATTGGGCGGTTCGCCATGCTGGCATCTCCGGCGACCGCGCt                                                                                   >  4:283721/1‑139 (MQ=255)
                                            ttAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTGGGCGTTTCGCAATGCTCTCATCTCCGGCTACcgcg                                                                                     <  3:190346/137‑1 (MQ=255)
                                            ttAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTGGGCGTTTCGCAATGCTCTCATCTCCGGCTACcgcg                                                                                     <  7:369624/137‑1 (MQ=255)
                                            ttAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTGGGCGTTTCGCAATGCTCTCATCTCCGGCTACcgcg                                                                                     <  4:264495/137‑1 (MQ=255)
                                            ttAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTGGGCGTTTCGCAATGCTCTCATCTCCGGCTACCgcgc                                                                                    <  5:388584/138‑1 (MQ=255)
                                            ttAATGAAGCGCCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCGCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTGGGCGTTTCGCAATGCTCTCATCTCCGGCTACcgcg                                                                                     <  1:114788/137‑1 (MQ=255)
                                                    gcgcCGCTCACTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTTGGCCCCATTTTTTTAAAATGGTCAGTCACACCCTTCATTGGGCGTTTAGCAATTCGGAAATCACCGGCTACCGCGCGGTGAAAAg                                                                           >  3:26976/1‑139 (MQ=255)
                                                             aCTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTGGGCGTTTCGCAATGCTCTCATCTCCGGCTACCGCGCTGTAAAAAGGACGGcc                                                                    <  6:78998/137‑1 (MQ=255)
                                                             aCTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTGGGCGTTTCGCAATGCTCTCATCTCCGGCTACCGCGCTGTAAAAAGGACGGcc                                                                    <  3:401969/137‑1 (MQ=255)
                                                              cTGACAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTGGGCGTTTCGCAATGCTCTCATCTCCGGCTACCGCGCTGTAAAAAGGATGGCCCGc                                                                 <  6:399061/139‑1 (MQ=255)
                                                                 aCAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCAGTTTTTTCAAAGGCGCCGGCACCCGCGTCCTTGGGCGTTTCGCAATGGGCTCCTGTCCGGGTACCCCGCGGTTAAAAGGCCGGCCCGCCaa                                                              >  4:149133/1‑139 (MQ=255)
                                                                  cAGCGGTGTAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTGGGCGTTTCGCAATGCTCTCATCTCCGGCTACCGCGCTGTAAAAAGGACGGCCCGCCaat                                                             <  4:314213/139‑2 (MQ=255)
                                                                       gtgtAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCGGTCACACGCTTCATTGGGCGTTTGGCAATGCTCTCATCTCCGGCTACCGCGCTGTAAAAAGGACGGCCCGCCAATATTcc                                                        >  5:169035/1‑139 (MQ=255)
                                                                        tgtAAACTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTGGGCGTTTCGCAATGCTCTCATCTCCGGCTACCGCGCTGTAAAAAGGACGGCCCGCCAATATTCCg                                                       <  3:426648/139‑1 (MQ=255)
                                                                                  ccgccgGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTAAAAAGCTCCGTCACCCCCATCATTGGGCGTTTCGCAATGCTCTCATATACGGCTACCCCGCAGTAAAAAAGACGGCCCCCCAAAAAACCCAGCATCAGGc                                             >  4:144481/1‑139 (MQ=255)
                                                                                  ccgccgGCTCTGCCGTCGAGTTTAGGCCCCAGTTTTTTAAAAGGATGAGTCACACCCTTAATTTGGCGGTTCGCAAAGCTCACAAATCCGGCAACCCCGCGGTAAAAAAGAAGGCCCGCCAAAATTACGAGCAtcatgc                                             >  8:360130/1‑134 (MQ=255)
                                                                                    gcgggCTGTGCCGGCGATTTTAGCCCCCATTTTTTACAATGGCTCAGTCACACGCTTCATTGGGCGTTTCGAAATGCTCTCATCTCGGGCTACCGCGCTTTAAAACGCACGGCCCGCCAATATTCCGAGCATCAGGCGa                                           <  2:387846/136‑1 (MQ=255)
                                                                                    gccgGCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCATTGGGCGTTTCGCAATGCTCTCATCTCCGGCTACCGCGCTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGa                                           <  4:359983/139‑1 (MQ=255)
                                                                                        gCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCGCAGTCACCCGCGTCATTGGGCGTTTCGCAATGCTCTCCTCTCCGGCTTCCGCGCTGGAAAAAGGCCGGCCCGCCCATATTGCGAGCATCAGGCGAATCg                                       >  3:130236/1‑139 (MQ=255)
                                                                                        gCTCTGCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCGCAGTCACACGCTTCATTGGGCGTTTCACAATGCTCTCATCTCCGGCTACCGCGCTGTAAAACGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCg                                       <  4:151129/139‑1 (MQ=255)
                                                                                         ctaggCCGTCGATTTTAGCCCCCATTTTTTTCAAAGGCTCAGTCACACGCTTCTTTGGGCGTTTCGCAATGCTCTCATCTCCGGCTACCGCGCTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGt                                      <  2:73258/135‑1 (MQ=255)
                                                                                                                   tttttCAAAGGCTCAGTCACACGCTTCATTGGGCGTTTCGCAATGCTCTCATCTCCGGCTACCGCGCTGTAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTaaa             <  7:52876/135‑1 (MQ=255)
                                                                                                                             gCTCCGTCACACGCTTCATTGGGCGTTTCGCATTGCTCTCATCTCCGGCTACCGCGATGTAAAACGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTgg  <  7:360130/139‑1 (MQ=255)
                                                                                                                             gCTCAGTCCCACGCTTCATTGGGGGTTTCGAAATGCTCTCATCTCCGGCTACCGCGCTGAAAAACGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTgg  <  4:26976/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                   
TTTAATTTGTGCGCTTGCAACAGGTGATACATAATCAATGCCTTTTAATGAAGCGCCGCTCACTGACAGCGGTGTAAATTCTCCGCCGGCTCTGCCGTCGATTTTAGCCCCCATTTCCTTCAAAGGCTCAGTTACACGTTTCATTGGGCGCTTCGCAATGCTCTCATCTCCGGCTACCGCGCTGTAAAAAGGACGGCCCGCCAAAATGCCGAGCATGAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGG  >  CP006881/2186597‑2186860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑