Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,186,910	T→C	N64S (AAC→AGC)	U712_10955 ←	3‑phosphoshikimate 1‑carboxyvinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,186,910	0	T	C	100.0%	226.0 / NA	70	N64S (AAC→AGC)	U712_10955	3‑phosphoshikimate 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/31);  total (39/31)

CTGTAAAAAGGACGGCCCGCCAAAATGCCGAGCATGAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCACTCAGGGAATCGATTCCATTTCCGTGAATCACTACATTGCTGCCGTTTTGCTCAATTTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCCGCTCCCGGCAGAAAGTTTTTCACAGTTGTTGTGCCCTTCGCTAGCGCGCCAAACATGACGGAACGGTGAGAAAT  >  CP006881/2186778‑2187040                                                                                                                                     |                                                                                                                                   cTGTACAAAGGACGGCCCGACAATATTCCGAGCATCAGTCGAATCCTTGTACCTGAATTTCCGACATCTAACAGGCGTTCTGGCTCTTTCAGGGCATCGCTTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCt                                                                                                                              <  2:44219/139‑1 (MQ=255)    tAAAACGGCCGGCCCGCCAATAATCCGAGCATCAGGCGAATCGTAGTACCTGAATTTCAGACATCTAAAAGGCTTTCTGGCTCATTCAGGGCCTCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTc                                                                                                                             <  1:392831/137‑1 (MQ=255)    tAAAAAGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCGTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCaa                                                                                                                           <  7:226707/139‑1 (MQ=255)    tAAAAAGGAAGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCTACATCTAAAAGGCTTTCTGGCTCTTTCAGTGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTc                                                                                                                             <  8:7151/137‑1 (MQ=255)         aGGACGGCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAAtgtg                                                                                                                       >  6:336619/1‑138 (MQ=255)          ggACGGCCCGCGAATAGTCCGAGCAGCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGGATCGATTCCTTTTCCGGTAATCACGACATCGCTGCTGCGTTGCTCAATGTGaa                                                                                                                     <  4:52958/139‑1 (MQ=255)              ggCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGCCATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACAcc                                                                                                                 >  6:153261/1‑139 (MQ=255)              ggCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCTATGTGAACAcc                                                                                                                 >  8:445822/1‑139 (MQ=255)              ggCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACAcc                                                                                                                 >  1:122822/1‑139 (MQ=255)              ggCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACAcc                                                                                                                 >  7:182493/1‑139 (MQ=255)              ggCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACAcc                                                                                                                 >  2:5478/1‑139 (MQ=255)              ggCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACAcc                                                                                                                 >  7:305091/1‑139 (MQ=255)              ggCCCGCCAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACAcc                                                                                                                 >  7:259418/1‑139 (MQ=255)              ggCCCGCCAATATTCCGAGCATCAGGCGAATAGTTGTACATGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGGACAcc                                                                                                                 >  5:173554/1‑139 (MQ=255)                     cAATATTCCGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCAtttt                                                                                                           >  2:138213/1‑138 (MQ=255)                        tattccGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTa                                                                                                       <  5:336619/135‑1 (MQ=255)                          ttccGAGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTa                                                                                                       >  8:121982/3‑137 (MQ=255)                               aGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAat                                                                                                <  6:189388/139‑1 (MQ=255)                               aGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAat                                                                                                >  7:108449/1‑139 (MQ=255)                               aGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACAACCATTTTTCTAAAGCAat                                                                                                >  4:295379/1‑139 (MQ=255)                               aGCATCAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTGTCAGGGCATCGATTCGTTTTCCGTGAATCACGACATCGATGATGCTTTGCTCAATGTGAACACCGTTTTTTCTAAAGAAat                                                                                                >  4:293198/1‑139 (MQ=255)                                   tcaGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAatcgat                                                                                            <  4:130236/137‑1 (MQ=255)                                   tcaGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCAGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAatcgat                                                                                            <  6:276678/137‑1 (MQ=255)                                    caGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAatcgatc                                                                                           >  4:20245/2‑139 (MQ=255)                                    caGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAatcgatc                                                                                           >  5:346565/2‑139 (MQ=255)                                          aaTCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTc                                                                                     <  7:121982/139‑1 (MQ=255)                                                tGTACCTGAATTTACGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTAGCTCAATGTGAACACCCATTTATCTAAAGCAAACGATCGTGCTCAGACa                                                                                >  3:157610/1‑138 (MQ=255)                                                        aaTTTCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTc                                                                        <  1:138213/138‑1 (MQ=255)                                                          tttCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCg                                                                     >  7:158916/1‑139 (MQ=255)                                                            tCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTaa                                                                                                      <  3:242509/104‑1 (MQ=255)                                                            tCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTaa                                                                                                      >  4:242509/1‑104 (MQ=255)                                                            tCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCgg                                                                    >  4:112831/1‑138 (MQ=255)                                                            tCCGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCgg                                                                    >  7:211433/1‑138 (MQ=255)                                                              cGACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCgg                                                                    >  3:37058/1‑136 (MQ=255)                                                               gACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGGACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGa                                                                <  6:173554/139‑1 (MQ=255)                                                               gACATCTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGa                                                                <  8:211433/139‑1 (MQ=255)                                                                    cTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTa                                                                                                       <  1:376539/95‑1 (MQ=255)                                                                    cTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTa                                                                                                       >  2:376539/1‑95 (MQ=255)                                                                    cTAAAAGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAATGtt                                                           >  7:47262/1‑139 (MQ=255)                                                                         aGGCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGtttttaa                                                      <  8:158916/139‑3 (MQ=255)                                                                           gCTTTCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTaact                                                    <  1:114363/139‑2 (MQ=255)                                                                              ttCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGa                                                                <  3:100418/124‑1 (MQ=255)                                                                              ttCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTgtt                                                 >  4:379438/1‑139 (MQ=255)                                                                              ttCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCAATTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGa                                                                >  4:100418/1‑124 (MQ=255)                                                                               tCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGtttttaa                                                      <  4:187752/133‑3 (MQ=255)                                                                               tCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGtttttaa                                                      >  3:187752/1‑131 (MQ=255)                                                                               tCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTgttg                                                >  5:178919/1‑139 (MQ=255)                                                                               tCTGGCTCTTTCAGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTgttg                                                >  1:152939/1‑139 (MQ=255)                                                                                   gctctttcaGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTgttgtt                                              <  8:182493/131‑1 (MQ=255)                                                                                      ctttcaGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGcc                                         >  8:337236/4‑139 (MQ=255)                                                                                      ctttcaGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGcc                                         >  4:142062/4‑139 (MQ=255)                                                                                         tcaGGGCATCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGcc                                         <  3:112831/136‑1 (MQ=255)                                                                                                 tCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGa                                                                <  1:88299/105‑1 (MQ=255)                                                                                                 tCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGa                                                                >  2:88299/1‑105 (MQ=255)                                                                                                 tCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAgcg                              <  8:266131/139‑1 (MQ=255)                                                                                                 tCGATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAgcg                              >  2:285922/1‑139 (MQ=255)                                                                                                   gATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAgcgcg                            <  1:389875/139‑1 (MQ=255)                                                                                                   gATTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAgcgcg                            <  1:194218/139‑1 (MQ=255)                                                                                                    aTTCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAgcgcgc                           >  6:428019/1‑139 (MQ=255)                                                                                                     ttCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGcc                          <  6:346565/139‑1 (MQ=255)                                                                                                     ttCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGcc                          <  8:115960/139‑1 (MQ=255)                                                                                                     ttCCTTTTCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGcc                          <  3:144481/139‑1 (MQ=255)                                                                                                         ttttCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATATGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGACTGCAGCTAGCGCGCCaaa                       >  8:406894/2‑138 (MQ=255)                                                                                                          tttCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCTGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCatcc                      >  5:237749/1‑135 (MQ=255)                                                                                                           ttCCGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCAAACa                     >  7:15953/1‑138 (MQ=255)                                                                                                             ccGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTa                                                                                                       >  4:121758/1‑54 (MQ=255)                                                                                                             ccGTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTa                                                                                                       <  3:121758/54‑1 (MQ=255)                                                                                                               gTGAATCACGACATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCAAACAtaa                  >  4:146550/1‑135 (MQ=255)                                                                                                                      acgaCATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCAAACATAACAGAGCGGt         <  5:428019/136‑1 (MQ=255)                                                                                                                      acgaCATCGCTGCTGCTTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGATCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCTCCAAACATAACAGAGCGGt         <  7:406894/136‑1 (MQ=255)                                                                                                                             cgctgctgctTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGAGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTCTTGTTGTGCCTGCCGCTAGCGCGCCAAACATAACATAGCGGTGAGAAAt  >  2:109452/2‑139 (MQ=255)                                                                                                                              gctgctgctTTGCTCAATGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAAt  <  8:259418/138‑1 (MQ=255)                                                                                                                                     |                                                                                                                                   CTGTAAAAAGGACGGCCCGCCAAAATGCCGAGCATGAGGCGAATCGTTGTACCTGAATTTCCGACATCTAAAAGGCTTTCTGGCTCACTCAGGGAATCGATTCCATTTCCGTGAATCACTACATTGCTGCCGTTTTGCTCAATTTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCCGCTCCCGGCAGAAAGTTTTTCACAGTTGTTGTGCCCTTCGCTAGCGCGCCAAACATGACGGAACGGTGAGAAAT  >  CP006881/2186778‑2187040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑